EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,<br />
BIOSYNTHESIS, FEED AND FOOD SAFETY<br />
P29 - Different levels <strong>of</strong> fumonisin production and<br />
FUM gene cluster expression on 2B710Hx corn hybrid<br />
G. M. Reis 1 , L. O. Rocha 1 , V. M. Barroso 1 , L. J. Andrade 1 , B. Corrêa 1<br />
1 University <strong>of</strong> São Paulo, Biomedical Sciences Institute, Avenue Pr<strong>of</strong>essor Lineu Prestes, 1374, São<br />
Paulo-Brazil<br />
E-mail: gabrielamreis@usp.br<br />
Maize is the third most cultivated crop worldwide, and Brazil is currently, the<br />
fourth largest producer in the world. Fusarium verticillioides is commonly<br />
associated with maize and it is capable to produce high levels <strong>of</strong> fumonisins.<br />
Studies regarding the use <strong>of</strong> transgenic corn have shown that fumonisin<br />
contamination could be reduced due to the damage decrease caused by insects<br />
on grains. The aim <strong>of</strong> this study was to evaluate the effect <strong>of</strong> cry 1F toxin<br />
produced by Bacillus thuringiensis subsp. aizawai, expressed in the 2B710Hx<br />
(Herculex) corn hybrid on the fumonisin production and on FUM gene expression<br />
by F. verticillioides ICB 13BA (NCBI accession number; GU 989110). 2B710 Hx<br />
corn samples and its respective isogenic non-Bt were previously irradiated with 20<br />
kGy in order to eliminate fungi contaminants from field. Plates were inoculated<br />
with 5 x 10 8 spores/mL and incubated at 25 o C and Aw <strong>of</strong> 0.99. After 10, 20 and<br />
30 days, samples were analysed regarding FB1 and FB2 contamination and<br />
FUM1, FUM3, FUM6, FUM7, FUM8, FUM10, FUM11, FUM13, FUM14, FUM15,<br />
FUM17, FUM18, FUM19 and FUM21 gene expression. The statistical analyses<br />
were performed using the s<strong>of</strong>twares R 2.9, Gamlss package and SAS 9.1. The<br />
results showed reduced fumonisin B1 contamination on 2B710 Hx during the 30<br />
days <strong>of</strong> analyses (p< 0.005). Regarding FB2, there was no statistical difference<br />
between the isogenic and transgenic hybrids. There was no correlation between<br />
FUM gene cluster and fumonisin production according to Pearson’s correlation<br />
test (except for FUM19, p