EFS12- Book of abstracts - Contact

SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,
BIOSYNTHESIS, FEED AND FOOD SAFETY
P27 - Fumonisins occurrence in maize cobs infected
with opposite mating type strains of F. verticillioides
M. Wit 1 , P. Ochodzki 2 , A. Waskiewicz 2 , R. Warzecha 2 , P. Goliński 3 , E.
Jablonska 1 , W. Wakulinski 1
1 Department of Plant Pathology, Warsaw University of Life Sciences SGGW, Nowoursynowska 159,
02-766 Warsaw, Poland; 2 Plant Breeding and Acclimatization Institute, 05-870 Blonie, Poland;
3 Department of Chemisty, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznan,
Poland
E-mail: wojciech_wakulinski@sggw.pl
Fusarium verticillioides is along with F. subglutinans and F. proliferatum the
principal causative factors of pink ear rot. Occurrence of the species is variable
and strongly related to high temperature and limited precipitation. F. verticillioides
is hetherothalic fungus with dimictic mating system governed by two idiomorphs
MAT1-1 and MAT1-2. The strains of opposite mating types are widespread in
Poland and frequency of their occurrence is statistically equivalent. Diverse
phenotypic traits of opposite mating strains of some fungi inclined us to verify
fumonisins accumulation in maize cobs inoculated with MAT1-1 and MAT1-2 F.
verticillioides strains.
Material and methods: Ten genotypes of four maize varieties (Zea mays var.
indentata, Zea mays var. indurata, Zea mays var. saccharata and Zea mays var.
everta) were used in these studies. Cobs were inoculated 7 days after silk
emergence with 2 ml conidial suspension of opposite mating type of the fungus.
Cobs Infection degree was evaluated according to 6 degree scale while ergosterol
and fumonisins content were determined by HPLC method detailed described
elsewhere.
Results: During four years of field studies, it was found that neither infection
degree of particular Zea mays varieties determined phenotypically and by
ergosterol content nor Fumonisins level were not dependent on MAT type. All the
mentioned factors were significantly influenced by environmental condition of
cropping season, maize variety and kernel composition (especially water,
amylose, amylopectin content).
Acknowledgment: The work was financed by the Ministry of Science and Higher
Education, project No. N N310 376933 and participation in 12 th European
Fusarium Seminar is supported through 7FP-Regpot-2011-1-286093
Keywords: fumonisin, mating types
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