EFS12- Book of abstracts - Contact

SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,
BIOSYNTHESIS, FEED AND FOOD SAFETY
P21 - Correlation between Fusarium DNA and
mycotoxin levels in Finnish oats samples
T. Yli-Mattila 1 , T. Hussien 1,3 , A. L. Carlobos-Lopez 1,4 , S. Rämö 2 , V.
Hietaniemi 2
1 Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku,
20014, Turku, Finland; 2 MTT Agrifood Research Finland, Laboratories, 31600 Jokioinen, Finland;
3 Mycotoxins Lab, Department of Food Toxicology and Contaminant, National Research Center, Dokki,
Cairo, Egypt; 4 University of the Philippines Los Baños, Laguna, Philippines
E-mail: tymat@utu.fi
The highest levels of deoxynivalenol (DON) were found in Finnish oats in 2012,
when about 12-24 % of oats samples contained >1750 ppb of DON. In our
samples of the years 2011 and 2012 DON and T-2/HT-2 levels varied from 0-
10000 ppb of DON and 0-3000 ppb of T-2/HT-2 toxins. The DNA levels were also
compared to DON values obtained by using the RIDA®QUICK SCAN kit. In these
samples DON levels were between 530->5500 ppb.
The coefficient of determination (R 2 ) between F. graminearum DNA and DON
levels was 0.95*** in the 25 oats samples of the year 2011 and 0.73*** in the 38
oats samples of the year 2012 obtained from MTT, while the R 2 value from the 20
oats samples from a food company was only 0.22*. In the latter case the DON
level was estimated by using the RIDA®QUICK SCAN, while in MTT the mycotoxin
levels were measured by using ROMER MycoSep-GC-MS and the grain flour
was sieved through the 1 mm sieve in order to homogenize it before the analysis.
The sieved flour was also used for DNA measurements, while for the samples
from the food company the flour was not homogenized by sieving. No correlation
was found between F. culmorum DNA and DON levels. F. graminearum DNA
levels were in all cases in agreement with DON levels, when DON was measured
by GC-MS. When compared to RIDA®QUICK SCAN kit results (DON) the variation
in DNA levels was much higher. There was also a significant correlation between
the combined T-2 and HT-2 and combined F. langsethiae and F. sporotrichioides
DNA levels (0.33 * in 2011 and 0.89*** in 2012). According to our results F.
graminearum is clearly the main DON producer in Finnish oats, while F.
langsethiae is probably the main T-2/HT-2 producer.
Keywords: F. graminearum, F. langsethiae, TaqMan qPCR, DON
113