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SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,
BIOSYNTHESIS, FEED AND FOOD SAFETY
P20 - Changes in fungal biomass and fumonisin
production by Fusarium proliferatum strains in the
presence of host plant extracts
Ł. Stępień 1* , A. Waśkiewicz 2 , K. Wilman 1
1 Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy
of Sciences, Strzeszyńska 34, 60-479 Poznań, Polan; 2 Department of Chemistry, Poznań University of
Life Sciences, Wojska Polskiego 75, 60-625 Poznań, Poland
E-mail: lste@igr.poznan.pl
The main objective of present study was to examine the impact of the aqueous
extracts from host plant species: asparagus (A), maize (M), garlic (G) and
pineapple (P), on the growth and fumonisin biosynthesis by F. proliferatum
isolates originating from the respective host species.
Strains were grown in 100 ml flasks containing liquid medium. Liquid media were
collected in 2 day intervals and subjected to FBs quantification. Fourteen-day-old
cultures were transferred into the pre-weighted falcons, the mycelia were
centrifuged and freeze-dried. The amount of dry weight was measured and the
contents of FBs were measured in liquid media and in dried mycelia.
Three strains yielded very small amounts of mycelium (below 20 mg of dry weight
after 14 days of culture). As a consequence, the highest inducing effects of four
plant extract tested were observed for those strains with the top values of over 20fold
increase (P extract), 10-fold increase (A extract), 9-fold increase (M extract)
and 30-fold increase (G extract). Likewise, the biomass of the strains of the best
yield in control conditions remained unchanged in the cultures with plant extract
added. For other strains, the dry weight of the mycelium with extracts added on
the 5th day of culturing increased as much as 20-fold (4-fold on average) in the
case of a pineapple extract. Extracts from asparagus and maize induced about
two-fold-increase of fungal biomass and garlic extract about 1.5-fold increase.
Weak negative correlation was found between biomass amount in the presence of
the extract and amount of fumonisins synthesized, especially considering A
extract. For the majority of isolates it caused dramatic inhibition of FBs synthesis.
Maize extract increased the amount of the toxin and garlic extract caused
differential effects depending on the isolate and host origin.
Research was supported by the Polish National Science Centre Project
2011/01/B/NZ8/00162.
Keywords: F. proliferatum, fumonisins, host-pathogen interaction
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