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SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY, BIOSYNTHESIS, FEED AND FOOD SAFETY P17 - Semiochemical interactions between toxigenic Fusarium fungi and insects T. Y. Gagkaeva, O. P. Gavrilova, I. V. Shamshev, O. G. Selitskaya, E. I. Savelieva All-Russian Institute of Plant Protection (VIZR), Sh. Podbelskogo 3, 196608, St.-Petersburg – Pushkin, Russia E-mail: t.gagkaeva@yahoo.com The aim of the study was to evaluate chemical nature and functional abilities of semiochemical interactions between toxigenic Fusarium fungi and insects. An analysis of variations of Fusarium fungi (F. graminearum, F. culmorum, F. cerealis, F. poae, F. sporotrichioides, F. langsethiae) in formation of olfactory responses of insects (rice weevil Sitophilus oryzae) was performed. The evaluation of olfactory stimulation with Fusarium fungi on test-insects was carried out after growing of the strains on two substrates: potato sucrose agar and autoclaved wheat grains. In the laboratory experiments F. langsethiae and F. poae strains have shown the clear attractive effect to the tested insects. The pathogenic to plants F. graminearum and F. culmorum strains have demonstrated the repellent effect. Generally beetles of rice weevil responded similar reaction to the cultures of fungal strains growing on both substrates. The method of solid-phase microextraction was used to clarify the spectra of volatile organic compounds above the surface of living fungal cultures. Screeningportraits of metabolomic profiles of Fusarium strains after 7 days of cultivation were obtained. A characteristic profile of sesquiterpenes, that are isomeric carbohydrates with molecular mass 204 and are genetically connected with trichodien, is richest in F. langsethiae in compare with another Fusarium spp. The culture of F. langsethiae produced maximal amount of trichodien also actively emitted volatile compounds. Under equilibrium condition of steam above surface of F. langsethiae, F. sporotrichioides и F. poae cultures were identified ethylacetate, isobutyl, isoamyl and amyl alcohols. Amylacetate and isoamylacetate, ethers of acetic acid and corresponding amyl alcohols were found only in the culture of F. poae. Most likely just these chemicals responsible for a sweet fruit smell that is typical for this fungus. The investigation was supported by the project No. 12-04-00927-а of the Russian Foundation for Basic Research. Keywords: Fusarium, insects, sesquiterpenes, olfactory stimulation 109
SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY, BIOSYNTHESIS, FEED AND FOOD SAFETY P18 - Effect of cry 1Ab toxins on FUM gene cluster expression and on fumonisin production by Fusarium verticillioides L. O. Rocha 1 , G. M. Reis 1 , V. M. Barroso 1 , L. J. Andrade 1 , B. Corrêa 1 1 University of São Paulo, Biomedical Sciences Institute, Avenue Professor Lineu Prestes, 1374, São Paulo-Brazil E-mail: lilianarocha@usp.br F. verticillioides is most commonly associated with maize worldwide and produces high levels of fumonisins, one of the most important mycotoxins. Previous studies have demonstrated that cry 1Ab protein from Bacillus thuringiensis sbsp. kurstaki could be suitable for Fusarium toxins management due to the control of crop insect injuries. The aim of this study was to assess the impact of cry 1Ab protein expressed in the 30F35YG (YieldGard) corn hybrid on FUM gene expression and on fumonisin production by F. verticillioides ICB 13BA (GU 989110/NCBI accession number). We also evaluated the influence of B. thuringiensis sbsp. kurstaki spores and cry 1Ab crystals on the F. verticillioides growth. In order to eliminate the original microbiota, Bt corn samples and its respective non-Bt isogenic were previously irradiated with 20 kGy. Plates were inoculated with 5 x 10 8 F. verticillioides spores/mL and incubated at 25 o C and relative humidity of 97.5%. After 10, 20 and 30 days, samples were analysed regarding FB1 contamination and expression of 14 FUM genes. The statistical analyses were performed using the softwares R 2.9, Gamlss package and SAS 9.1. An initial fungal inoculum of 5 x 10 8 spores/mL was cultured with 10 μg/mL of bacterial spores and crystals. F. verticillioides biomass was estimated by dry weight of mycelia during 1, 3 and 5 days . The results showed reduced fumonisin B1 contamination on 30F35 YG in comparison to its isogenic non-Bt corn during the periods of the analyses (p= 0.0095, Weibull distribution). There was no correlation between FUM gene cluster and fumonisin production according to Pearson’s correlation test (except for FUM19, p
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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