EFS12- Book of abstracts - Contact

SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,
BIOSYNTHESIS, FEED AND FOOD SAFETY
P16 - Volatile compounds in grain of various wheat
cultivars naturally infected and inoculated with
Fusarium culmorum
M. Buśko 1 , K. Stuper 1 , T. Góral 2 , A. Ostrowska 1 , J. Perkowski 1
1 Poznań University of Life Sciences, Department of Chemistry, Wojska Polskiego str. 75, 60-637
Poznań Poland; 2 Department of Plant Pathology, Plant Breeding and Acclimatization Institute NRI,
Radzików, 05-870 Blonie, Poland;
E-mail: mabu@up.poznan.pl
In the present work 30 winter wheat cultivars were investigated for the volatile
organic compounds (VOCs). The metabolic profile was established for both
healthy and inoculated with Fusarium culmorum grain. VOCs were extracted by
mean of HS-SPME method and further analyzed using GC-MS. In samples of
grain growing in natural conditions and after inoculation with F. culmorum several
dozens of compounds were qualitatively determined. They belong to terpenes,
alcohols, aldehydes and ketones, benzene derivatives and hydrocarbons. On the
base of obtained chromatograms and mass spectra after deconvolution and
estimation of retention indexes 42 compounds were identified.
The concentration sum of VOCs for controlled and inoculated samples was
similar. However, for particular compounds groups it was differentiated. In
controls a higher concentration was stated than in inoculated samples for
benzene derivatives, hydrocarbons and terpenes. On the other hand, alcohols,
aldehydes and ketones prevailed in inoculated samples. In all of groups, except of
terpenes, obtained trends were equal. Above observations for terpenes confirm
their key significance in toxic metabolites biosynthesis by Fusarium. These
observations also confirm the fact, that lack or low concentrations of particular
terpenes in profile of VOCs are related with rich profile of other terpenes.
Among determined terpenes there were 6 the most important for trichothecenes
biosynthesis: α-pinene, 3-carene, indane, β-chamigrene, thujopsene and
trichodiene. For α-pinene, 3-carene, indane a higher concentration was stated in
control samples whilst for β-chamigrene, thujopsene and trichodiene in inoculated
samples.
The most important are results obtained for trichodiene, which is intermediate
metabolite in trichothecenes biosynthesis. It cannot be excluded that other
terpenes are significant for this metabolomic pathway and could be useful for
understanding mechanism of Fusarium toxins biosynthesis.
This work was partially supported by the National Science Center in Poland
project no. 2704/B/P01/2011/40
Keywords: Fusarium, volatiles, wheat, SPME
108