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SESSION 1: FUSARIUM – GENETICS, GENOMICS AND SYSTEMS BIOLOGY P11 - Identifying indicators of soil suppressiveness to fungal diseases K. Siegel 1 , S. Aimé 1 , E. Chapelle 2 , V. Edel-Hermann 1 , J. Raaijmaakers 2 , P. Lemanceau 1* , C. Steinberg 1 1 INRA, UMR1347 Agroécologie, 17 rue Sully, BP 86510, 21065 Dijon, France; 2 Wageningen University; Bld 107, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands; * Coordinator of the EU project Ecofinders FP7-ENV-2010-264465 E-mail: christian.steinberg@dijon.inra.fr Soils suppressive to soil-borne diseases are defined by a low disease incidence in spite of the presence of a virulent pathogen and a susceptible plant. In many cases, the inhibition of the disease development relies on the activity of the resident soil microbiome. To identify taxonomic microbial indicators linked to the suppressiveness phenotype of soils, culture independent-based methods have been employed to analyse and compare microbial dynamics in two different soils suppressive to either Rhizoctonia solani damping-off disease of sugar beet or Fusarium wilt disease on flax. Fungal and bacterial taxonomic biodiversity were estimated from ITS and 16S genes by amplicon pyrosequencing. To that end, metagenomic DNA was extracted from the rhizosphere of plants grown in soils with different level of suppressiveness. We obtained 218650 reads in total (125602 for fungi and 93048 for bacteria). At this moment, the analyses of fungal communities are in progress. 114641 reads was kept after filtering by bioinformatic pipeline, distributed into 2303 clusters and 3379 singletons. Although, the bioinformatic and statistical analysis are not finished yet, we have already noticed a difference in the taxonomic diversity composition between suppressive and conducive soils which could explain the suppressive/conducive character of given soil. The next step is to achieve the bacterial communities in Fusarium wilt suppressive/conducive soils and to assess the microbial diversity of other soilborne diseases suppressive soils. Once the analyses of sequencing data are finished and the taxonomic assignments done, the comparison of microbial diversity of all studied soils will be performed in order to find out the similarities or/and differences in these soils which will provide the suppressiveness indicators. Keywords: Châteaurenard, Ecofinders, Fusarium oxysporum, Soil-borne disease 103
SESSION 1: FUSARIUM – GENETICS, GENOMICS AND SYSTEMS BIOLOGY P12 - Disentangling mycotoxin regulatory pathways in Fusarium graminearum by quantitative genetics B. Laurent, N. Ponts, V. Atanasova-Penichon, C. Barreau, M. Foulongne- Oriol INRA UR1264 MycSA, 71 Avenue Edouard Bourlaux, CS20032, 33882 Villenave d'Ornon, France E-mail: mfoulong@bordeaux.inra.fr Fusarium graminearum is a major causal agent of Fusarium Head Blight, and Maize Ear Rot. During infection, this fungus produces extremely stable trichothecene mycotoxins that accumulate in grains, such as deoxynivalenol, or DON. This toxin represents a threat for human and animal consumers, and maximum levels of contamination of cereals commercialized in Europe are now strictly regulated. The biosynthetic pathway leading to trichothecene accumulation has been well described but the genetic determinism behind the regulation of toxin production remains, however, largely unknown. To begin to answer this question, we have initiated an original approach of quantitative genetic: how many quantitative trait loci (QTL) are involved in the regulation of toxin production, what are their effects, where are they located on the genome, do they interact with each other? So far, there is no published data suitable to perform QTL analyses for toxin production in F. graminearum. We started our search for QTLs influencing DON accumulation using the intraspecific progeny that has been previously generated for creating the reference linkage map (Gale et al. 2005, Genetics), and in which toxin levels segregated as a polygenic trait. Alignment of the map with the genome will allow the identification of candidate genes that may underlie these QTLs. In addition, we have initiated the creation of a new segregating progeny adapted for the quantitative analyses of several traits related to toxin production. Upon completion, this work will provide sound basis to understand the biology and the genetic of trichothecene production in Fusarium, and open new possibilities to elaborate innovative strategies to combat this pathogen. Keywords: Fusarium, mycotoxin, biosynthesis, QTL 104
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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SESSION 6: FUTURE CHALLENGE FOR EUR
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de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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