EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 1: FUSARIUM – GENETICS, GENOMICS AND SYSTEMS BIOLOGY<br />
P10 - Interspecific Hybrids between Fusarium fujikuroi<br />
and Fusarium proliferatum<br />
N. M. I. Mohamed Nor¹ , ², B. Salleh¹, C. P. Toomajian², J. P. Stack², J. F.<br />
Leslie²<br />
¹School <strong>of</strong> Biological Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia; ²Department<br />
<strong>of</strong> Plant Pathology, Kansas State University, Manhattan, Kansas 66506-5502, USA<br />
E-mail: jfl@ksu.edu<br />
Interspecific hybrids <strong>of</strong>fer unusual opportunities to study speciation and the<br />
segregation <strong>of</strong> traits that differ between species but that are <strong>of</strong>ten fixed within a<br />
species. We have recovered strains that appear to be hybrids between F.<br />
fujikuroi and F. proliferatum from rice fields in Southeast Asia and from a native<br />
tallgrass prairie in the United States. These hybrids can cross with standard<br />
mating type testers <strong>of</strong> both species and have DNA sequence pr<strong>of</strong>iles that are<br />
consistent with their putative hybrid conditions. The existence <strong>of</strong> these hybrids<br />
may indicate that reticulate evolution is occurring or that these two species have<br />
yet to completely finish the speciation process. We also have created such<br />
hybrids under laboratory conditions by crossing strains <strong>of</strong> opposite mating type on<br />
carrot agar. Based on the segregation <strong>of</strong> AFLPs, there are some fingerprint<br />
patterns that reoccur independently multiple times amongst the progeny,<br />
suggesting non-random segregation <strong>of</strong> at least portions <strong>of</strong> the genome. The<br />
parental strains differ in numerous traits, including secondary metabolite<br />
production and pathogenicity towards apples, onions and rice. Evaluation <strong>of</strong><br />
recombinant progeny is providing the opportunity to identify portions <strong>of</strong> the<br />
genome involved in the speciation process and to identify regulatory and<br />
structural genes <strong>of</strong> importance in pathogenicity and secondary metabolite<br />
production.<br />
Keywords: speciation, hybridization, secondary metabolites, recombination<br />
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