EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 1: FUSARIUM – GENETICS, GENOMICS AND SYSTEMS BIOLOGY<br />
P5 - Comprehensive inventory on coding and noncoding<br />
features <strong>of</strong> the genome <strong>of</strong> Fusarium fujikuroi.<br />
C. Sieber 1 , M. Münsterkötter 1 , P. Wiemann 2,4 , B. Tudzynski 2 , M. Hippler 3 , S. V.<br />
Bergner 3 , T. Bald 3 , U. Güldener 1<br />
1<br />
Institute <strong>of</strong> Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research<br />
Center for Environmental Health, 85764 Neuherberg, Germany; 2 Institut für Biologie und<br />
Biotechnologie der Pflanzen, Molecular Biology and Biotechnology <strong>of</strong> Fungi, Westfälische Wilhelms-<br />
Universität Münster, Schlossplatz 8, 48143 Münster, Germany;<br />
3 Institut für Biologie und<br />
Biotechnologie der Pflanzen, Plant Biochemistry and Biotechnology, Westfälische Wilhelms-<br />
Universität Münster, Schlossplatz 8, 48143 Münster, Germany; 4 Department <strong>of</strong> Medical Microbiology<br />
and Immunology, University <strong>of</strong> Wisconsin-Madison, WI, United States<br />
E-mail: christian.sieber@helmholtz-muenchen.de<br />
The plant pathogen Fusarium fujikuroi synthesizes a vast array <strong>of</strong> secondary<br />
metabolites and causes „bakanae“ disease on rice. Here we present the fully<br />
annotated genome sequence <strong>of</strong> F. fujikuroi assembled in 12 contigs which<br />
correspond to 12 chromosomes. Compared to closely related fungi <strong>of</strong> the<br />
Gibberella fujikuroi species complex (GFC) considerable differences regarding the<br />
presence <strong>of</strong> secondary metabolism gene clusters are highlighted, and some <strong>of</strong><br />
them might be linked to host specificity <strong>of</strong> the respective species. Utilizing DNA<br />
microarray and proteomics experiments we demonstrated a nitrogen and pH<br />
specific expression and translation.<br />
A deeper analysis <strong>of</strong> the genome sequence revealed a diversity <strong>of</strong> previously<br />
unknown interspersed repeat families which can be found in high frequency all<br />
over the genome. While some <strong>of</strong> the families occur exclusively in F. fujikuroi,<br />
others distribute throughout the GFC. DNA microarray experiments demonstrated<br />
that some elements are part <strong>of</strong> the transcriptome and still seem to propagate<br />
further in the genome. Moreover, some elements seem to have a preference to<br />
appear between collinear blocks.<br />
Main questions are: What influence do interspersed repeats have on genome<br />
structure and the speciation process <strong>of</strong> fungi? Do repeats contribute to hostpathogen<br />
interaction and specification?<br />
Keywords: Fusarium, fujikuroi, noncoding, secondary-metabolism, regulation<br />
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