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SESSION 1: FUSARIUM – GENETICS, GENOMICS AND SYSTEMS BIOLOGY
P5 - Comprehensive inventory on coding and noncoding
features of the genome of Fusarium fujikuroi.
C. Sieber 1 , M. Münsterkötter 1 , P. Wiemann 2,4 , B. Tudzynski 2 , M. Hippler 3 , S. V.
Bergner 3 , T. Bald 3 , U. Güldener 1
1
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research
Center for Environmental Health, 85764 Neuherberg, Germany; 2 Institut für Biologie und
Biotechnologie der Pflanzen, Molecular Biology and Biotechnology of Fungi, Westfälische Wilhelms-
Universität Münster, Schlossplatz 8, 48143 Münster, Germany;
3 Institut für Biologie und
Biotechnologie der Pflanzen, Plant Biochemistry and Biotechnology, Westfälische Wilhelms-
Universität Münster, Schlossplatz 8, 48143 Münster, Germany; 4 Department of Medical Microbiology
and Immunology, University of Wisconsin-Madison, WI, United States
E-mail: christian.sieber@helmholtz-muenchen.de
The plant pathogen Fusarium fujikuroi synthesizes a vast array of secondary
metabolites and causes „bakanae“ disease on rice. Here we present the fully
annotated genome sequence of F. fujikuroi assembled in 12 contigs which
correspond to 12 chromosomes. Compared to closely related fungi of the
Gibberella fujikuroi species complex (GFC) considerable differences regarding the
presence of secondary metabolism gene clusters are highlighted, and some of
them might be linked to host specificity of the respective species. Utilizing DNA
microarray and proteomics experiments we demonstrated a nitrogen and pH
specific expression and translation.
A deeper analysis of the genome sequence revealed a diversity of previously
unknown interspersed repeat families which can be found in high frequency all
over the genome. While some of the families occur exclusively in F. fujikuroi,
others distribute throughout the GFC. DNA microarray experiments demonstrated
that some elements are part of the transcriptome and still seem to propagate
further in the genome. Moreover, some elements seem to have a preference to
appear between collinear blocks.
Main questions are: What influence do interspersed repeats have on genome
structure and the speciation process of fungi? Do repeats contribute to hostpathogen
interaction and specification?
Keywords: Fusarium, fujikuroi, noncoding, secondary-metabolism, regulation
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