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70 Biotechnological Approaches for Pest Management and Ecological Sustainability<br />

The responses of insects to volatile stimuli have been studied for several insects. Several<br />

designs of olfactometers have been used to observe insect behavior (Traynier, 1967; Sharma<br />

and Franzmann, 2001). Olfactory responses are also studied physiologically by electroantenograms<br />

(Visser, Van Straten, and Maarse, 1979; Guerin and Visser, 1980; Guerin,<br />

Stadler, and Buser, 1983; Saxena and Okech, 1985; Khan, Ciepiela, and Norris, 1987), electroretinograms,<br />

and by electronic feeding monitors (McLean and Weigt, 1968).<br />

Selection Indices<br />

In addition to low insect damage and/or low insect numbers on the resistant genotypes,<br />

several crops possess the ability to recover from insect damage and produce the same or<br />

more yield under insect infestation, for example, in the case of H. armigera damage in<br />

chickpea, pigeonpea, and cotton, certain genotypes have the ability to produce a second<br />

fl ush when the fi rst fl ush is heavily damaged. In the case of damage by shoot fl y, A. soccata<br />

and spotted stem borer, C. partellus in sorghum, the plants have the ability to produce axial<br />

tillers when the main plant is damaged, depending on the availability of nutrients and<br />

water in the soil. In some cases, the resistant genotypes have the ability to withstand higher<br />

insect numbers or damage. Several indices can be used to measure plant resistance or<br />

tolerance to insects based on insect numbers or damage and yield under infested and<br />

uninfested conditions. Some of the indices that can be used to select genotypes based on<br />

one or more damage parameters, and yield under infested and uninfested conditions are<br />

discussed below.<br />

Tolerance Index<br />

Tolerance to insect damage can be measured in terms of plant damage per unit number<br />

of insects.<br />

No. of insects per plant<br />

Tolerance rating _____________________<br />

Damage rating .<br />

This system identifi es the cultivars that suffer relatively low insect damage despite having<br />

a high insect population. N. Panda and Heinrichs (1983) calculated tolerance and<br />

antibiosis indices based on insect dry weight.<br />

Insect dry weight on the test cultivar<br />

Tolerance ________________________________________<br />

Insect dry weight on the susceptible cultivar .<br />

Loss in Grain Yield<br />

Tolerance to insect damage can also be measured in terms of loss in grain yield. Loss in<br />

grain yield of a test genotype under insect infestation relative to that under uninfested<br />

conditions is measured as:<br />

Loss in grain yield (%) [GYI _______________<br />

UN – GYIIN] 100<br />

GYIUN

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