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46 Biotechnological Approaches for Pest Management and Ecological Sustainability<br />

differences in the fl owering times of different genotypes within a crop. Because of fl uctuations<br />

in insect populations over the crop-growing season, it becomes diffi cult to obtain<br />

uniform insect pressure on genotypes fl owering at different times. Genotypes fl owering at<br />

the beginning and end of the cropping season escape insect damage, while those fl owering<br />

in the mid-season are exposed to heavy insect damage. As a result, it becomes diffi cult to<br />

make meaningful comparisons between the resistant and susceptible genotypes. To overcome<br />

this problem, it is important to group the test material according to maturity and<br />

height. It is equally important to include resistant and susceptible checks of appropriate<br />

maturity in different trials for proper comparison (Sharma et al., 1999, 2005a; B. Singh and<br />

Yadav, 1999a, 1999b).<br />

Sequential Plantings<br />

Even with the best of insect forecasting models, it is not possible to pinpoint the periods<br />

of maximum abundance of an insect under fi eld conditions. At the same time, it is not<br />

possible to take up timely planting of the test material because of the variation in the onset<br />

of rainfall in different seasons and locations. As a result, it becomes diffi cult to coincide<br />

the susceptible stage of the crop with maximum insect abundance because of changes<br />

in weather conditions, and the effect of environment on both the insect and the crop.<br />

Therefore, the test material should be planted two to three times at an interval of 15 to<br />

20 days so that one of the plantings is exposed to adequate insect damage. Such an approach<br />

also helps to reduce the chances of escape from insect damage as the late-fl owering genotypes<br />

in the fi rst planting and the early-fl owering genotypes in the second planting are most<br />

likely to fl ower during the mid-season, and are exposed to maximum insect abundance<br />

(Sharma, Singh, and Nwanze, 1997).<br />

Selective Control of Nontarget Insects<br />

Resistance screening trials are quite often rendered useless because of interference by the<br />

nontarget insect pests. Selective insecticides can be used either to control other insects infesting<br />

the crop at the same or at different times or to kill the natural enemies of the target insect<br />

species to allow population build up of the target insect (Sharma, Vidyasagar, and Leuschner,<br />

1988a). It is important to protect the cotton crop from jassid damage during the seedling<br />

stage to evaluate the test material for resistance to bollworms. Sorghum at the seedling stage<br />

(5 to 10 days old) can be sprayed with insecticides to control the shoot fl y to avoid interference<br />

with screening for resistance to spotted stem borer. Similarly, sorghum and pigeonpea<br />

can be sprayed at the milk stage to control the sucking pests to avoid interference in screening<br />

for resistance to sorghum midge and pod borer, respectively. Insecticide application at<br />

times also leads to resurgence of some insect species, for example, brown planthopper,<br />

Nilaparvata lugens (Stal), in rice (Chelliah and Heinrichs, 1980; Heinrichs et al., 1982). Pesticide<br />

application kills the natural enemies and the insects then multiply at a faster rate in the<br />

absence of natural enemies. Selective insecticides can also be used as powerful tool in conserving<br />

and enhancing insect populations (Shepard, Carner, and Turnipseed, 1977).<br />

Augmentation of Insect Populations<br />

Insect infestation in the screening nursery can be increased by collecting indigenous insect<br />

populations from the surrounding areas and releasing them in the test plots. Insect abundance<br />

in the screening nursery can also be augmented by placing nondestructive light,

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