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Evaluation of Transgenic Plants and Mapping Populations for Resistance to Insect Pests 45<br />

Midge damage (%)<br />

25<br />

20<br />

15<br />

10<br />

5<br />

0<br />

IR+IP IR IP Control<br />

MD CS<br />

FIGURE 3.1 Effect of planting infester rows (IR) inoculated with sorghum panicles having diapausing larvae<br />

(IP) on sorghum midge, Stenodiplosis sorghicola damage under fi eld conditions. MD, Spikelets with midge<br />

larvae (%); CS, midge-damaged chaffy spikelets (%).<br />

should be removed after infestation of the test material has taken place so that it does not<br />

compete with the test material.<br />

Trap crops that are attractive to the target insect species can also be used to increase insect<br />

infestation in the test material (Stern, 1969). The H. armigera infestations can be increased by<br />

planting Solanum vairum Dunal in tomato (Talekar, Hau, and Chang, 1999), and sesame and<br />

pigeonpea in cotton (Sharma et al., 2005a). Mustard planted in cabbage and caulifl ower can be<br />

used to increase diamondback moth, Plutella xylostella (L.), infestation. Brassica juncea (L.) Czern.<br />

can be planted in cotton to increase the infestation by Lygus lineolaris (Palisat de Beauvois)<br />

(Laster and Meredith, 1974). When the main crops reach the susceptible stage, the trap crops<br />

are either allowed to senesce or are chopped off so that the insects migrate to the test material.<br />

Grouping the Material According to Maturity and Height<br />

To screen for resistance to insect species that feed on reproductive structures, it is<br />

important to group the test material according to maturity and height as there are large<br />

FIGURE 3.2 Infester row fi shmeal technique to screen sorghum for resistance to shoot fl y, Atherigona soccata.

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