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44 Biotechnological Approaches for Pest Management and Ecological Sustainability<br />

locations. As far as possible, efforts should be made to select locations where appropriate<br />

attention can be given to managing the trials and evaluation of the test material for resistance<br />

at the appropriate stage.<br />

Adjusting Planting Date<br />

The test material should be planted such that the most susceptible stage of the crop is<br />

exposed to maximum or optimum levels of insect infestation. Fortnightly or monthly<br />

plantings of a susceptible cultivar during the cropping season can also be used to determine<br />

the optimum time for planting the test material. Most of the crops planted 20 to<br />

25 days later than the normal planting times are exposed to heavy insect damage. The<br />

periods of maximum insect abundance can also be determined through monitoring of<br />

insect populations through light or pheromone traps. Sorghum crop planted during the<br />

second week of July in southern India is exposed to heavy damage by shoot fl y, A. soccata,<br />

sorghum midge, S. sorghicola, and head bug, Calocoris angustatus (Lethiery) (Sharma et al.,<br />

1992). Most of the crops (cotton, maize, pigeonpea, tomato, sunfl ower, and sorghum)<br />

planted during July in South Asia are exposed to heavy H. armigera infestations during the<br />

rainy season, while October is the optimum time to plant the test material (chickpea,<br />

tomato, and saffl ower) during the post-rainy season for resistance screening (Sharma et al.,<br />

2005a). Similar planting times should be determined for different insect pests and their<br />

crop hosts in each region to maximize the chances for obtaining adequate levels of insect<br />

infestation for resistance screening.<br />

Manipulation of Cultural Practices<br />

Cultural practices can be manipulated to increase the activity of certain insects in the fi eld<br />

(Hollingsworth and Berry, 1982). Closer spacing leads to greater incidence of rice gall<br />

midge, Orseolia oryzae Wood-Mason (Prakasa Rao, 1975). Lower planting densities lead to<br />

greater infestation of sorghum shoot fl y, A. soccata, and sorghum midge, S. sorghicola<br />

(Sharma et al., 1992). A drought-stressed sorghum crop suffers greater severity of spotted<br />

stem borer, Chilo partellus (Swinhoe) damage, while sprinkler irrigation during the fl owering<br />

stage increases the severity of sorghum midge and head bug, C. angustatus (Sharma<br />

et al., 1992). Fertilizer application increases the damage by planthopper, A. biguttula biguttula<br />

and bollworms, E. vittella and H. armigera in cotton.<br />

Planting Infester Rows<br />

Planting susceptible cultivars as infester rows along the fi eld borders or at regular intervals<br />

in the fi eld can be used to increase insect infestation in the test material. The infester<br />

rows may be planted 20 to 25 days earlier, or an early fl owering crop or cultivar can be<br />

used as infester rows so that the fl owering in the infester rows occurs earlier than the test<br />

material in the case of insects feeding on the reproductive parts (Figure 3.1). There should<br />

be suffi cient time for the insect to multiply on the infester rows, and then move to the test<br />

material (Sharma, Vidyasagar, and Leuschner, 1988a; Smith, Khan, and Pathak, 1994).<br />

Insects collected from nearby fi elds can also be released in the infester rows. Insects can<br />

also be attracted to the infester rows by kairomones (e.g., fi shmeal for sorghum shoot fl y)<br />

(Figure 3.2). Plant material with diapausing insects (e.g., chaffy sorghum panicles with<br />

midge larvae or stalks of cereal crops with diapausing larvae of stem borers) can also be<br />

spread in the infester rows to increase the severity of insect damage. The infester rows

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