Contents - Faperta

480 Biotechnological Approaches for Pest Management and Ecological Sustainability
TABLE 17.1
PCR Primers Used for Detecting Parasitoids
Species Family Region References
Ageniaspis citricola Encyrtidae Actin genes Hoy et al. (2000)
Aphelinus abdominalis (Dalman) Aphelinidae Microsatellite Vanlerberghe-Masutti and
Chavigny (1997)
Aphelinus hordei ITS-2 Zhu et al. (2000)
ITS-2, 16s
rDNA
Prinsloo et al. (2002)
Aphidius ervi CO I-II, CO B Daza-Bustamante et al. (2002)
Microsatellite Hufbauer, Bogdanowicz, and
Perez (2001)
Cotesia congregata (Say) Braconidae Microsatellite Jensen et al. (2002)
Diaeretiella rapae Microsatellite Loxdale and MacDonald (2004)
Lydella thompsoni Tachinidae CO I Agusti et al. (2005)
Lysiphlebus testaceipes Aphidiidae ITS-2 Persad, Jayaprakash, and Hoy (2004)
Nasonia vitripennis (Walker) Pteromalidae ITS-1 Ratcliffe et al. (2002)
Peristenus digoneutis Loan Pteromalidae ITS-1, ITS-2 Erlandson et al. (2003)
Pseudoperichaeta nigrolineata Tachinidae CO I Agusti et al. (2005)
Stomodys calcitrans (L.) Pteromalidae ITS-1 Ratcliffe et al. (2002)
Trichomalopsis sarcophagae (Gahan) Pteromalidae ITS-1 Ratcliffe et al. (2002)
Trichogramma australicum (Girault) Trichogrammatidae ITS-2 Amornsak, Gordh, and Graham
(1998)
ITS-1, ITS-2 Sappal et al. (1995)
Trichogramma deion Pinto and
Oatman
ITS-2 Stouthamer et al. (2000)
Trichogramma dendrolimi
Matsumura
ITS-2 Li and Shen (2002)
Trichogramma minutum Riley ITS-1, ITS-2 Sappal et al. (1995)
Trichogramma ostriniae Pang
et Chen
ITS-1 Chang et al. (2001)
Trichogramma pretiosum Riley ITS-2 Stouthamer et al. (2000)
Trichogramma turkestanica Meyer ITS-2 Silva et al. (1999)
McIntosh and Lysiphlebius testaceips (Cresson), within the bodies of their aphid hosts.
Tilmon et al. (2000) used PCR-RFLP of mitochondrial COI gene to detect parasitism of
mirid bugs, Lygus lineolaris (Palisot de Beauvois) by Peristenus spp. Species-specifi c ITS2
and 16S primers have been used to determine the establishment of the parasitoid, Aphelinus
hordei Kurd. for the control of Russian wheat aphid, Diuraphis noxia (Mord.) (Prinsloo et al.,
2002), and of the eggs of L. lineolaris by Anaphes iole Girault (Zhu and Williams, 2002).
Detection of Insect Biotypes
Molecular tools have been used for solving routine taxonomic and ecological problems
regarding biotype or cryptic status of insect pests. Sequences of ITS2 region have been
used to separate the Australian and Taiwanese Ageniaspis fuscicollis (Dalman) populations
(Alvarez and Hoy, 2002). Molecular markers have been found to be useful for characterizing
strains to determine genetic origin and identity of the parasitoids for use in pest management.