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308 Biotechnological Approaches for Pest Management and Ecological Sustainability<br />

persist in new geographic areas, indicated that M. occidentalis cannot persist through the<br />

wet season, although it may be able to establish and persist through the autumn, winter,<br />

and spring months.<br />

Conclusions<br />

Molecular approaches will play an important role in exploring insect phylogeny and diversity,<br />

while genetic engineering will enable the production of insects with known functions.<br />

Biotechnological tools offer the potential of genetically modifying the insects for disease<br />

control, production of vaccines, more robust natural enemies with resistance to pesticides,<br />

or better adaptation to extremes of climatic conditions, increased host range, and effi cient<br />

rearing systems. Although considerable progress has been made in the laboratory, these<br />

advances have not been accompanied by product development, delivery, and risk assessment.<br />

Experience with plant biotechnology may serve as a guideline, and also as a warning,<br />

if the public does not understand completely the benefi ts of the development of<br />

genetically modifi ed insects for health, agriculture, and industrial purposes. There is a<br />

need to develop mechanisms to drive the gene of interest through natural populations, testing,<br />

deployment, and risk assessment.<br />

References<br />

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expression vector in mosquito cells. Experimental Parasitology 79: 322–339.<br />

Afanasiev, B.N., Ward, T.W., Beaty, B.J. and Carlson, J.C. (1999). Transduction of Aedes aegypti<br />

mosquitoes with vectors derived from Aedes densovirus. Virology 257: 62–72.<br />

Aksoy, S. (2000). Tsetse: A haven for microorganisms. Parasitology Today 16: 114–118.<br />

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Atkinson, P.W. and O’Brochta, D.A. (1999). Genetic transformation of non-drosophilid insects by<br />

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Atkinson, P.W., Hines, E.R., Beaton, S., Matthaei, K.I., Reed, K.C. and Bradley, M.P. (1991). Association<br />

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Baldarelli, R.M. and Lengyel, J.A. (1990). Transient expression of DNA after ballistic introduction into<br />

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