Contents - Faperta

Genetic Engineering of Entomopathogenic Microbes for Pest Management 279
However, biosafety concerns have been raised regarding the deployment of the genetically
engineered biocontrol agents for pest management. There is a need for a thorough investigation
regarding the fate of genetically modifi ed organisms in the environment, and their
interaction with wild relatives and nontarget organisms. Products based on Bt at times
may not be compatible with some pesticides. For example, some insecticides have a signifi -
cant antifeeding effect, while a Bt preparation has to be ingested for a desired effect.
Genetically engineered microbial pesticides can play an important role in pest management
and reduce the amounts of pesticides applied for pest control, leading to reduction of
pesticide residues in food and food products, and resulting in conservation of the environment.
A fast-track process can be adopted for testing and release of genetically engineered
microbes, which are known to give satisfactory results and have a relatively safer track
record in different environments.
References
Abbaiah, K., Satyanarayanan, A., Rao, K.T. and Rao, N.V. (1988). Incidence of fungal disease on
Heliothis armigera larvae in Andhra Pradesh, India. International Pigeonpea Newsletter 8: 11.
Adams, L.F., Mathewes, S., O’Hara, P., Petersen, A. and Gürtler, H. (1994). Elucidation of the mechanism
of CryIIIA overproduction in a mutagenized strain of Bacillus thuringiensis var. tenebrionis.
Molecular Microbiology 14: 381–389.
Agaisse, H. and Lereclus, D. (1994). Expression in Bacillus subtilis of the Bacillus thuringiensis cryIIIA
toxin gene is not dependent on a sporulation-specifi c sigma factor and is increased in a spo0A
mutant. Journal of Bacteriology 176: 4734–4741.
Agaisse, H. and Lereclus, D. (1995). How does Bacillus thuringiensis produce so much insecticidal
crystal protein? Journal of Bacteriology 177: 6027–6032.
Aima, P.J. (1975). Infection of pupae of Heliothis armigera by Paecilomyces farinosus. New Zealand Journal
of Forestry Science 5: 42–44.
Anderson, C. (1992). Researchers ask for help to save key pesticide. Nature 355: 661.
Anderson, L. and Lewis, L.C. (1991). Suppression of Ostrinia nubilalis (Hubner) (Lepidoptera:
Pyralidae) by endophytic Beauveria bassiana (Balsamo) Vuillemin. Environmental Entomology 20:
1207–1211.
Aronson, A.I., Han, E.S., McGaughey, W. and Johnson. D. (1991). The solubility of inclusion proteins
from Bacillus thuringiensis is dependent upon protoxin composition and is a factor in toxicity to
insects. Applied Environmental Microbiology 57: 981–986.
Atkinson, H. (1993). Opportunities for improved control of plant parasitic nematodes via plant biotechnology.
In Beadle, D.J., Copping, D.H.L., Dixon, G.K. and Holloman, D.W. (Eds.),
Opportunities for Molecular Biology in Crop Production. Farnham, UK: British Crop Protection
Conference, 257–266.
Bar, E., Lieman Hurwitz, J., Rahamim, E., Keynan, A. and Sandler, N. (1991). Cloning and expression
of Bacillus thuringiensis israelensis d-endotoxin in B. sphaericus. Journal of Invertebrate Pathology 57:
149–158.
Bateman, R., Carey, M., Moore, D. and Prior, C. (1993). The enhanced infectivity of Metarhizium
fl avoviride in oil formulations to desert locusts at low humidities. Annals of Applied Biology 122:
145–152.
Baum, J.A. (1994). Tn5401, a new class II transposable element from Bacillus thuringiensis. Journal of
Bacteriology 176: 2835–2845.
Baum, J.A., Kakefuda, M. and Gawron Burke, C. (1996). Engineering Bacillus thuringiensis bioinsecticides
with an indigenous site-specifi c recombination system. Applied Environmental Microbiology
62: 4367–4373.