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Contents - Faperta

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x <strong>Contents</strong><br />

7. Genetic Transformation of Crops for Resistance to Insect Pests .......................... 209<br />

Introduction ...................................................................................................................... 209<br />

Genetic Transformation: The Protocols ........................................................................ 210<br />

Agrobacterium-Mediated Gene Transfer ................................................................... 210<br />

Microprojectile Bombardment with DNA or Biolistics ......................................... 210<br />

Direct DNA Transfer into Isolated Protoplasts ....................................................... 210<br />

Gene Expression ............................................................................................................... 211<br />

Genetic Transformation of Crop Plants for Resistance to Insects ............................ 214<br />

Toxin Proteins from Bacillus thuringiensis ................................................................ 214<br />

Cotton ....................................................................................................................... 217<br />

Maize ........................................................................................................................ 219<br />

Rice ........................................................................................................................... 219<br />

Sorghum .................................................................................................................. 220<br />

Sugarcane ................................................................................................................ 220<br />

Oilseed Crops ......................................................................................................... 221<br />

Grain Legumes ....................................................................................................... 221<br />

Tobacco ..................................................................................................................... 221<br />

Potato ........................................................................................................................ 222<br />

Vegetables ................................................................................................................ 223<br />

Fruits and Forest Trees .......................................................................................... 223<br />

Ornamentals ........................................................................................................... 223<br />

Vegetative Insecticidal Proteins ................................................................................ 224<br />

Toxin Proteins from Photorhabdus luminescens ........................................................ 224<br />

Secondary Plant Metabolites ..................................................................................... 224<br />

Protease Inhibitors ...................................................................................................... 225<br />

Tobacco ..................................................................................................................... 226<br />

Potato ........................................................................................................................ 228<br />

Cotton ....................................................................................................................... 228<br />

Maize ........................................................................................................................ 228<br />

Rice ........................................................................................................................... 229<br />

Wheat ....................................................................................................................... 229<br />

Sugarcane ................................................................................................................ 229<br />

Vegetables ................................................................................................................ 229<br />

Chrysanthemum .................................................................................................... 230<br />

Alpha Amylase Inhibitors .......................................................................................... 230<br />

Enzymes ....................................................................................................................... 231<br />

Plant Lectins ................................................................................................................ 232<br />

Tobacco ..................................................................................................................... 232<br />

Potato ........................................................................................................................ 233<br />

Cotton ....................................................................................................................... 233<br />

Cereals ...................................................................................................................... 233<br />

Sugarcane ................................................................................................................ 233<br />

Vegetables ................................................................................................................ 234<br />

Viruses, Neurotoxins, and Insect Hormones .......................................................... 234<br />

Neurotoxins ............................................................................................................ 234<br />

Viruses ..................................................................................................................... 234<br />

Neuropeptides and Peptidic Hormones ............................................................. 234<br />

Biotin-Binding Proteins ......................................................................................... 235<br />

Antibodies .................................................................................................................... 236<br />

Inducible Resistance ........................................................................................................ 236

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