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OPERATION<br />
<strong>SW</strong> <strong>28</strong> <strong>and</strong> <strong>SW</strong> <strong>28</strong>.1 Rotors<br />
Figure 1. Arranging Tubes Symmetrically in the Rotor Buckets. Two, three,<br />
four, or six tubes can be centrifuged per run if they are arranged in the rotor<br />
as shown. All buckets must be attached to the rotor, whether loaded or empty.<br />
For low-temperature runs, precool the rotor in the centrifuge or in a<br />
refrigerator before use—especially before short runs—to ensure that<br />
the rotor reaches the set temperature. A suggested precooling cycle is<br />
a minimum of 30 minutes at 2000 rpm at the required temperature.<br />
1. To install the rotor, carefully lift it up off the rotor st<strong>and</strong> with both<br />
h<strong>and</strong>s—do not lift the rotor by the adapter—<strong>and</strong> place it on the<br />
drive hub. Slowly turn the rotor to the right (clockwise) to make<br />
sure that the rotor is seated properly on the hub.<br />
! CAUTION<br />
Remove the zonal support b<strong>and</strong> from the ultracentrifuges<br />
so equipped before operating these<br />
rotors.<br />
2. Refer to the centrifuge instruction manual for additional operating<br />
instructions.<br />
➠ NOTE<br />
Some gradients may degrade when run time<br />
exceeds 8 hours.<br />
7
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