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<strong>SW</strong> <strong>28</strong> <strong>and</strong> <strong>SW</strong> <strong>28</strong>.1 Rotors<br />
18<br />
Homogeneous CsCl Solution (g/mL)<br />
1.90<br />
1.80<br />
1.70<br />
1.60<br />
1.50<br />
1.40<br />
1.30<br />
1.20<br />
1.10<br />
0<br />
1/4<br />
1/4<br />
1/2<br />
1/2<br />
3/4<br />
3/4<br />
full<br />
<strong>SW</strong> <strong>28</strong>.1 ROTOR<br />
= 20°C<br />
= 4°C<br />
full<br />
5 10 15 20 25 <strong>28</strong><br />
Rotor Speed (K rpm)<br />
Figure 4. Precipitation Curves for the <strong>SW</strong> <strong>28</strong>.1 Rotor.<br />
Using speed <strong>and</strong> density combinations that intersect on or below the solid curves ensures that CsCl will not precipitate<br />
during centrifugation. Tube fill volumes are indicated on the curves. The dashed line are a representation of<br />
equation (6) <strong>and</strong> are shown here to illustrate the inability of that equation to prevent CsCl precipitation.<br />
full<br />
3/4<br />
1/2 1/4
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