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Density (g/mL)<br />
1.9<br />
1.8<br />
1.7<br />
1.6<br />
1.5<br />
1.4<br />
1.3<br />
1.2<br />
1.1<br />
1.0<br />
75.3<br />
r min<br />
15 000 rpm<br />
15 000 rpm<br />
20 000 rpm<br />
20 000 rpm<br />
25 000 rpm<br />
25 000 rpm<br />
<strong>28</strong> 000 rpm<br />
<strong>28</strong> 000 rpm<br />
3/4 filled tube<br />
1/2 filled tube<br />
96.7 118.2<br />
Distance from Axis of Rotation (mm)<br />
139.6<br />
Figure 3. CsCl Gradients at Equilibrium for the <strong>SW</strong> <strong>28</strong> Rotor.<br />
Centrifugation of homogeneous CsCl solutions at maximum allowable speeds<br />
(from Figure 2) results in gradients presented here.<br />
1/4 filled tube<br />
<strong>SW</strong> <strong>28</strong> ROTOR<br />
Each square on the grid<br />
represents 1.26 mm by<br />
0.01 g/mL.<br />
<strong>SW</strong> <strong>28</strong> <strong>and</strong> <strong>SW</strong> <strong>28</strong>.1 Rotors<br />
= 20°C<br />
= 4°C<br />
161.0<br />
r max<br />
17
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