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SW 28 and SW 28.1 ROTORS

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<strong>SW</strong> <strong>28</strong> <strong>and</strong> <strong>SW</strong> <strong>28</strong>.1 Rotors<br />

16<br />

Homogeneous CsCl Solution (g/mL)<br />

1.90<br />

1.80<br />

1.70<br />

1.60<br />

1.50<br />

1.40<br />

1.30<br />

1.20<br />

1.10<br />

0<br />

1/4<br />

1/2<br />

<strong>SW</strong> <strong>28</strong> ROTOR<br />

= 20°C<br />

= 4°C<br />

1/4<br />

3/4<br />

1/2<br />

full<br />

5 10 15 20 25 <strong>28</strong><br />

Rotor Speed (K rpm)<br />

Figure 2. Precipitation Curves for the <strong>SW</strong> <strong>28</strong> Rotor.<br />

Using speed <strong>and</strong> density combinations that intersect on or below the solid curves ensures that CsCl will not precipitate<br />

during centrifugation. Tube fill volumes are indicated on the curves. The dashed lines are a representation of<br />

equation (6) <strong>and</strong> are shown here to illustrate the inability of that equation to prevent CsCl precipitation.<br />

3/4<br />

full<br />

full<br />

3/4<br />

1/2 1/4

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