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www.sciencemag.org/cgi/content/full/317/5837/523/DC1<br />
<strong>Supporting</strong> <strong>Online</strong> <strong>Material</strong> <strong>for</strong><br />
Candidatus Chloracidobacterium thermophilum: An Aerobic Phototrophic<br />
Acidobacterium<br />
Donald A. Bryant, * Amaya M. Garcia Costas, Julia A. Maresca, Aline Gomez Maqueo<br />
Chew, Christian G. Klatt, Mary M. Bateson, Luke J. Tallon, Jessica Hostetler, William C.<br />
Nelson, John F. Heidelberg, David M. Ward<br />
*To whom correspondence should be addressed. E-mail: dab14@psu.edu<br />
This PDF file includes:<br />
<strong>Material</strong>s and Methods<br />
SOM Text<br />
Figs. S1 to S13<br />
Table S1<br />
References<br />
Published 27 July 2007, <strong>Science</strong> 317, 523 (2007)<br />
DOI: 10.1126/science.1143236
<strong>Supporting</strong> <strong>Online</strong> <strong>Material</strong> <strong>for</strong><br />
A phototrophic acidobacterium discovered by metagenomics<br />
D. A. Bryant*, A. M. Garcia Costas, J. A. Maresca, A. Gomez Maqueo Chew, C. Klatt, M. M.<br />
Bateson, L. J. Tallon, J. Hostetler, W. C. Nelson, J. F. Heidelberg, and D. M. Ward<br />
*To whom correspondence should be addressed. E-mail: dab14@psu.edu<br />
This supplement contains:<br />
<strong>Material</strong>s and Methods<br />
SOM text<br />
Figs. S1 to S13<br />
Table S1<br />
References<br />
1
<strong>Supporting</strong> <strong>Online</strong> <strong>Material</strong><br />
MATERIALS AND METHODS<br />
Library construction, DNA sequencing, and sequence analysis<br />
The sequence of a representative Roseiflexus sp. isolate, strain RS1 (1-3) was determined<br />
as part of a Joint Genome Institute-Department of Energy project to determine genome<br />
sequences of filamentous anoxygenic phototrophs (http://genome.jgi-psf.org/; GenBank<br />
accession AAQU00000000). The Synechococcus sp. OS-A and OS-B' genome sequences (4)<br />
(GenBank accessions CP000239 and CP000240) and metagenomic clone libraries and databases<br />
(4-6) used in this study were developed <strong>for</strong> an NSF Frontiers in Integrative Biology Research<br />
project (7). Amplicon Express (Pullman, WA) constructed the bacterial artificial chromosome<br />
(BAC) clone library. Sequence data derived from BAC clone M60-018 J19 (see Fig. 6S) have<br />
been deposited in GenBank under accession number EF531339.<br />
Sequencing and assembly of the metagenome were per<strong>for</strong>med as described <strong>for</strong> genomes<br />
sequenced by TIGR and the J. Craig Venter Institute (8). BAC closure was done by mechanical<br />
shearing of the entire BAC followed by random shotgun sequencing on small insert (2-3 kbp)<br />
clones. The plasmid sequences were jointly assembled using the TIGR Assembler (9). The<br />
coverage criteria were that every position required at least double-clone coverage (or sequence<br />
from a PCR product amplified from genomic DNA), and either sequence from both strands or<br />
with two different sequencing chemistries. The sequence was edited manually, and additional<br />
PCR (10) and sequencing reactions were per<strong>for</strong>med to close gaps, improve coverage, and resolve<br />
sequence ambiguities.<br />
2
An initial set of open reading frames likely to encode proteins (coding sequence, CDS)<br />
were predicted <strong>for</strong> the BAC insert by Glimmer (11). All predicted proteins larger than 30 amino<br />
acids were searched against a non-redundant protein database as previously described (12-14).<br />
TopPred was used to predict the membrane-spanning domains of proteins (15). The 5' regions of<br />
each CDS were inspected to define initiation codons using homologies, and position of<br />
ribosomal binding sites and transcriptional terminators. Two sets of hidden Markov models were<br />
used to determine CDS membership in families and superfamilies: pfam v11.0 (16) and<br />
TIGRFAMs 3.0 (17). Pfam v11.0 hidden Markov models were also used with a constraint of a<br />
minimum of two hits to find and mask repeated domains within proteins.<br />
Database searches were per<strong>for</strong>med using the various blast algorithms to search the<br />
GenBank non-redundant databases (18, 19). For construction of phylogenetic trees, protein<br />
sequences were aligned using the ClustalW tool in MacVector versions 7.2.3 or 9.0.2 (Accelrys,<br />
San Diego, CA). Neighbor-joining trees <strong>for</strong> type-1 reaction centers and BchU were generated<br />
from the alignments using PAUP 4.0 (Sinauer Associates, Inc., Sunderland, MA). The neighbor-<br />
joining tree <strong>for</strong> RecA was generated using MacVector version 9.0.2 (Accelrys, San Diego, CA).<br />
Enrichment culture methods and isolation of Anoxybacillus sp.<br />
Enrichment cultures containing Synechococcus sp. strain JA-2-3B'a (2-13),<br />
Chloracidobacterium (Cab.) thermophilum, and Anoxybacillus sp. (20) were grown in B-HEPES<br />
medium at pH 8.0 (21) at 45-53°C. In cultures to maintain and enrich <strong>for</strong> Cab. thermophilum, the<br />
B-HEPES medium was supplemented with 1 mM ammonium chloride and a mixture of carbon<br />
sources: lactate, succinate, glycolate, butyrate, propionate, and acetate (1 mM each, added in the<br />
<strong>for</strong>m of sodium salts). Nitrate was omitted from the growth medium of cultures that did not<br />
contain cyanobacteria. The medium was also supplemented with filter-sterilized vitamins at the<br />
3
following final concentrations: (10 µg L -1 ) riboflavin, vitamin B12 (100 µg L -1 ), thiamine-HCl<br />
(100 µg L -1 ), L-ascorbic acid (100 µg L -1 ), D-Ca-panthothenate (100 µg L -1 ), folic acid (100 µg<br />
L -1 ), nicotinic acid (100 µg L -1 ), 4-aminobenzoic acid (100 µg L -1 ), pyridoxine-HCl (100 µg L -1 ),<br />
and lipoic acid (100 µg L -1 ). To inhibit the growth of the cyanobacterial cells in the original<br />
enrichment, atrazine was added to a final concentration of 10 µM.<br />
Unless otherwise specified, all cultures were incubated under oxic conditions with or<br />
without shaking in constant light provided by a 60 W tungsten and a 60 W fluorescent bulb (total<br />
visible light intensity of 67 µmol photons m -2 s -1 ). Synechococcus sp. strain JA-2-3B'a (2-13) was<br />
eliminated from an enrichment that also contained Anoxybacillus sp. and Cab. thermophilum by<br />
serial subculturing three times in the light in the presence of 10 µM atrazine. Anoxybacillus sp.<br />
was isolated in axenic culture by plating cells from the enrichment cultures on Luria-Bertani<br />
medium solidified with 1.5% (w/v) Difco Bacto-Agar (Becton-Dickinson, Franklin Lakes, NJ).<br />
Anoxybacillus sp. was identified by amplifying and DNA sequencing a region of its 16S rRNA<br />
by using universal bacterial primers (Table 1S).<br />
Light microscopy<br />
Cab. thermophilum cells were incubated in 1-hexanol-saturated phosphate-buffered<br />
saline to disrupt bacteriochlorophyll (BChl) c aggregates (22) and visualized in an Olympus BX-<br />
60 epifluorescence upright microscope equipped with an UplandFL 100× oil objective, a<br />
mercury vapor lamp and Olympus filter cubes 41001 (Olympus, Center Valley, PA).<br />
Absorption and fluorescence emission spectra<br />
4
Absorption spectra were either recorded with a Cary 14 UV-Vis-NIR spectrophotometer<br />
modified <strong>for</strong> computerized data acquistion by On-Line Systems, Inc. (Bogart, GA) or with a<br />
GENESYS 10 spectrophotometer (Thermo Electron Corp., Rochester, NY). Data were analyzed<br />
using Igor-Pro software (Wavemetrics, Inc. Lake Oswego, OR).<br />
Chlorosome isolation and electron microscopy<br />
Chlorosomes were isolated from fresh cells (2 L) as described by Vassilieva et al. (23).<br />
Isolated chlorosomes were negatively stained with 1% (w/v) uranyl acetate and visualized in a<br />
JEOL 1200 EXII Transmission electron microscope (Peabody , MA).<br />
Pigment extraction and analysis<br />
Pigments were extracted from 1-ml aliquots of cell suspension from late-exponential<br />
phase enrichment cultures by sonication in acetone-methanol (7:2, v/v). Cell debris was removed<br />
by centrifugation. The absorption spectrum of the pigment mixture was recorded after diluting<br />
the sample at least 1:10 in methanol, and the absorption spectrum of the extracts was recorded<br />
from 350 to 900 nm. Samples <strong>for</strong> HPLC analysis were filtered and 0.1 volume of 1 M<br />
ammonium acetate was added to the filtrate immediately be<strong>for</strong>e injection into the HPLC column.<br />
The pigments were separated on a 25 cm by 4.6 mm Discovery 5 µm C18 column (Supelco,<br />
Bellefonte, PA) attached to a 1,024-element diode array detector (model G1315B, 1100 series;<br />
Agilent Technologies, Palo Alto, CA) controlled with Agilent ChemStation software <strong>for</strong> HPLC.<br />
The solvent system was previously described (24). BChl c and BChl a extracted from Chl.<br />
tepidum and from Cfx. aurantiacus Y-400-fl served as standards (see Fig. 4). Elution of the<br />
pigments was monitored at 667 and 770 nm, the absorbance maxima <strong>for</strong> BChl c and BChl a<br />
5
espectively; the absorption spectrum of the column eluate was recorded from 200 to 900 nm at<br />
two-second intervals.<br />
Polymerase chain reaction and agarose gel electrophoresis<br />
In general, the polymerase chain reaction conditions consisted of 5 minutes at 95 o C, 35<br />
cycles of 45 seconds at 95 o C, 45 seconds at 57 o C and 1 minute at 72 o C, followed by a final<br />
elongation step of 10 minutes at 72 o C. About 10-100 ng of DNA were included in each reaction<br />
and primers (see Table 1S) were supplied at a final concentration of 1 µM. PCR products were<br />
electrophoresed in a 0.8% (w/v) agarose gel and stained with ethidium bromide prior to<br />
visualization.<br />
SUPPORTING ON-LINE MATERIAL<br />
History of discovery of photosynthetic bacteria and their differentiating characteristics<br />
Five bacterial phyla (Cyanobacteria, Chlorobi, Proteobacteria, Chloroflexi, and<br />
Firmicutes) contain members, chlorophototrophs, capable of phototrophy based upon BChl or<br />
chlorophyll (Chl) (25). (Throughout this article, we have used the terms phylum and phyla as<br />
employed in the Second Edition of Bergey’s Manual of Systematic Bacteriology (26), although<br />
there is debate as to whether the major sublineages of Domains should be considered kingdoms,<br />
as in the case of plants, animals, and fungi.). Studies of Cyanobacteria and photosynthetic<br />
Proteobacteria (i.e. the purple bacteria) date to the first half of the 19 th century, and green sulfur<br />
bacteria (Chlorobi) were first described in 1906 (26, 27). Cyanobacteria per<strong>for</strong>m oxygenic<br />
photosynthesis by virtue of their Chl a-containing, type-1 (photosystem I) and type-2<br />
(photosystem II) reaction centers, and they fix carbon by the reductive pentose-phosphate<br />
6
(Calvin-Benson-Bassham) cycle. Endosymbiosis of a cyanobacterium led to the acquisition of<br />
oxygenic photosynthesis by eukaryotic algae and plants. The purple chlorophototrophic bacteria,<br />
all members of the alpha, beta, and gamma subdivisions of the Proteobacteria, have relatively<br />
simple, type-2 reaction centers with either BChl a or BChl b, do not evolve oxygen, and also fix<br />
carbon via the reductive pentose phosphate pathway, although most can grow mixotrophically<br />
when presented with light and a usable organic carbon source. Many purple bacteria are aerobic<br />
chemoheterotrophs under oxic conditions, but are only facultatively phototrophic under anoxic<br />
conditions. Chlorobi are strictly anaerobic, photoautotrophic organisms that have type-1 reaction<br />
centers with chlorosomes as antennae; any given strain can produce three types of Chl (Chl a,<br />
BChl a, and either BChl c, d, or e) and fix CO2 by the reverse TCA cycle.<br />
In the 100 years since the initial description of the Chlorobi (27), only two additional<br />
bacterial phyla have been shown to contain members that can produce BChls and reaction<br />
centers. Pierson and Castenholz (28) isolated the filamentous anoxygenic phototroph,<br />
Chloroflexus aurantiacus, from the same microbial mats of Octopus Spring and other thermal<br />
features around the world. This moderate thermophile synthesizes BChls a and c, produces<br />
chlorosomes as light-harvesting antennae, has type-2 reaction centers similar to those of purple<br />
bacteria, and fixes CO2 by the 3-hydroxypropionate cycle. Cfx. aurantiacus is the type species of<br />
the Chloroflexi, an early-diverging phylum of the Bacteria. Strains of both major subdivisions of<br />
the Chloroflexi contain the genes required <strong>for</strong> carbon fixation by the 3-hydroxypropionate cycle<br />
(2), although it should be noted that some chlorophototrophic Chloroflexi possess Rubisco and<br />
fix carbon by the reductive pentose-phosphate pathway (29). The most recently discovered<br />
reaction center-containing bacteria are the heliobacteria, which were originally described by Gest<br />
and Favinger in 1983 (30). Heliobacteria are Gram-positive bacteria, i.e., Firmicutes, which <strong>for</strong>m<br />
7
heat-resistant endospores and that are closely related to clostridia (see Fig. 8S). These strict<br />
anaerobes produce type-1 reaction centers containing BChl g, but no known isolate can grow<br />
photoautotrophically.<br />
Sequence analysis of a pscAB-fmoA operon recovered from the metagenome of the<br />
phototrophic mat community of alkaline siliceous hot springs<br />
Inserts encoding the highly divergent pscA gene of an organism from the phototrophic<br />
layer of the microbial mats of Octopus and Mushroom Springs in Yellowstone NP were<br />
completely sequenced from four independent plasmids and one BAC insert (see below; GenBank<br />
accession EF531339). The overlapping data revealed a probable operon, 5’pscAB-fmoA,<br />
encoding the type-1 reaction center (PscAB) and the FmoA apoprotein of the BChl a-binding<br />
Fenna-Matthews-Olson protein (Fig. 1B, Fig. 6S). Preliminary phylogenetic analyses of the<br />
flanking genes suggested that these DNA sequences were derived from an organism related to<br />
Acidobacterium sp. Ellin345 or Solibacter usitatus Ellin6076, two soil bacteria belonging to the<br />
poorly characterized phylum Acidobacteria (26), whose genomes have recently been sequenced<br />
(3).<br />
The three sequences deduced from the pscAB-fmoA gene cluster were divergent from<br />
known homologs in other anoxygenic chlorophototrophs. PscA was predicted to be a polypeptide<br />
of 865 amino acids (aa), which was ~135 aa larger than the PscA subunits of green sulfur<br />
bacterial type-1 reaction centers, to which it was most similar (31) (Fig. 1A, 1S). This reaction<br />
center apoprotein was more distantly related to PshA (~610 aa) of heliobacteria and the PsaA<br />
and PsaB subunits (730-750 aa) of cyanobacterial photosystem I (Figs. 1, 1S-3S). Like the<br />
apoproteins of all other type-1 reaction centers, the mat PscA was predicted to contain eleven,<br />
8
transmembrane α-helices. An insertion of ~165 aa between predicted transmembrane α-helices<br />
VII and VIII accounted <strong>for</strong> most of the size difference (Fig. 1B, 1S). This insertion corresponded<br />
to a large extrinsic loop, which might <strong>for</strong>m a docking site <strong>for</strong> a periplasmic electron donor to the<br />
oxidized special pair. The C-terminal electron transfer domain of PscA, comprising the last four<br />
transmembrane α-helices of the protein, was most similar to the same domain of green sulfur<br />
bacterial PscA sequences (Figs. 1, 3S), but this domain was nevertheless quite divergent (48%<br />
identical, 69% similar). However, the highly conserved motif, FPCIGPVKGGTCGVS,<br />
containing two cysteine residues in the cytoplasmic loop between transmembrane helices VIII<br />
and IX (Fig. 3S), implied that this reaction center contains an intersubunit [4Fe-4S] cluster<br />
similar to FX of PS I (31). The N-terminal, antenna domain of this PscA-like protein is not well<br />
conserved and was difficult to align to other reaction center sequences (Fig. 2S). It exhibits only<br />
~29% identity and ~43% similarity to the N-terminal antenna domain of PscA from green sulfur<br />
bacteria.<br />
Similar to PscB of green sulfur bacterial Type-1 reaction centers (31) and the PsaC<br />
protein of photosystem I (32, 33), the mat-derived PscB was predicted to be a small protein with<br />
eight conserved cysteines, which should ligate two terminal, electron-accepting, [4Fe-4S]<br />
clusters (see Fig. 4S). The region encompassing these cysteines was well conserved, but the<br />
remainder of the protein was much less similar to PscB sequences of green sulfur bacteria. The<br />
fmoA gene encodes the Fenna-Matthews-Olson protein, a BChl a-binding protein that occurs in<br />
all green sulfur bacteria (34). The fmoA gene is only known to occur in green sulfur bacteria, but<br />
in 12 sequenced green sulfur bacterial strains this gene is unlinked to pscAB operons. Although<br />
the FmoA proteins of green sulfur bacteria are highly similar in sequence (~70% identity, ~85%<br />
similarity), the pscAB-linked fmoA gene encoded a protein that is only ~40% identical and 58%<br />
9
similar to FmoA sequences of green sulfur bacteria (Fig. 5S). Based upon these data, we<br />
hypothesized that a thermophilic acidobacterium with a novel type-1 reaction center and FmoA<br />
occurs in the mats.<br />
Identification and sequencing of a bacterial artificial chromosome insert encoding pscAB-<br />
fmoA, recA, and the rDNA operon of an acidobacterium<br />
To compile more in<strong>for</strong>mation about the putative acidobacterium with type-1 reaction<br />
centers, metagenomic reads from ~60°C and ~65°C sites of the Octopus and Mushroom Spring<br />
microbial mats were assembled to produce consensus contigs. Most of the biomass in these mats<br />
belongs to organisms closely related to Synechococcus sp. OS-A and OS-B' and Roseiflexus sp.<br />
RS1 (35, 36), whose genomes have been sequenced (2-4, 37). Sequences showing >92% identity<br />
over 80% of the read to these three organisms were first removed. Contigs with G+C contents of<br />
~56 mol% and with multiple gene products showing best hits from blastx analyses to<br />
acidobacteria and/or δ-proteobacteria were then binned as possibly being derived from a single<br />
type of mat bacterium. This bin contained two scaffolds that encoded well-established<br />
phylogenetic markers: a complete recA gene and a partial 16S rRNA gene. Bacterial artificial<br />
chromosome (BAC) clones, constructed from DNA isolated from the photic-zone mat layer of<br />
the ~60°C Mushroom Spring site, were end-sequenced and mapped onto the binned scaffolds<br />
(Fig. 6S). These analyses predicted that the binned pscAB-fmoA, recA, and the rRNA genes were<br />
encoded on a single BAC insert. Sequencing of this 271,846 bp BAC insert (Fig. 6S) confirmed<br />
that the pscAB-fmoA and rRNA operons were separated by ~183 kbp with the recA gene between<br />
them. The complete 16S rRNA sequence derived from the BAC clone is identical to the partial<br />
sequence assembled from the metagenome and is nearly identical (differences at 2 of 1348<br />
10
shared positions) to that from an acidobacterium, denoted GFP1, the 16S rRNA sequence of<br />
which was recovered from Green Finger Pool in the Lower Geyser Basin of Yellowstone NP (a<br />
site ~5 km from Octopus Spring) (see Fig. 1 of 38) (Fig. 7S). These data unequivocally establish<br />
that the highly divergent pscAB-fmoA genes are derived from a GFP1-like organism belonging to<br />
the phylum Acidobacteria. Phylogenetic analyses of RecA are consistent with this conclusion<br />
(Fig. 8S, 9S).<br />
Analyses of sequences tentatively assigned to the GFP1-like acidobacterium.<br />
The binned sequence assemblies putatively derived from the GFP1-like acidobacterium<br />
were annotated by computer and analyzed. The acidobacterial sequence bin contained several<br />
genes <strong>for</strong> BChl and carotenoid biosynthesis as well as genes associated with chlorosomes. For<br />
example, bchU (Fig. 10S, 11S), whose product is a C-20 methyltransferase required <strong>for</strong> the<br />
synthesis of BChl c of green bacteria (39) and bchK, whose product is the BChl c synthase (40),<br />
were found. A radical-SAM-type methyltransferase similar to BchR, which methylates C-12 1 of<br />
BChl c (41), was also identified. These data predicted that the acidobacterium should produce<br />
methylated Bchl c homologs and assemble chlorosomes like green sulfur bacteria. Consistent<br />
with this inference, a csmA gene, whose product is a BChl a-binding protein of the chlorosome<br />
baseplate (42), was also found in a putative operon with bchG, encoding a BChl a synthase (see<br />
Fig. 6S). Overlapping metagenome scaffolds and BAC clones physically linked these genes and<br />
bchY, a subunit of chlorophyllide a reductase, to the genes on the sequenced BAC insert (Fig.<br />
6S). No oxygen-independent magnesium-protoporphyrin IX monomethyl ester oxidative cyclase<br />
(bchE) was identified. However, the presence of acsF, encoding an oxygen-dependent<br />
magnesium-protoporphyrin IX monomethyl ester oxidative cyclase (41, 43, 44), implied that the<br />
11
mat acidobacterium should synthesize BChl under oxic conditions (Fig. 12S). Genes with strong<br />
sequence similarity to crtB, crtP, and crtH further imply that the GFP1-like organism synthesizes<br />
carotenoids by a pathway similar to that in Chlorobi and Cyanobacteria (45). Although an mcr<br />
gene encoding malonyl-CoA reductase, an enzyme required <strong>for</strong> CO2 fixation by the 3-<br />
hydroxypropionate pathway (2), was identified, no other genes specifically attributable to CO2<br />
fixation were identified. No genes encoding hydrogenases or enzymes <strong>for</strong> sulfide/sulfur<br />
oxidation were detected.<br />
12
Supplemental Figure Legends<br />
Figure 1S. Diagram comparing the relative positions of transmembrane α-helices and loops of<br />
various Type-1 reaction center polypeptides. Bars connecting the tops of the rectangles represent<br />
loops in the cytoplasm and bars connecting the bottoms of the rectangles represent loops in the<br />
periplasm or thylakoid lumen. Note the very large periplasmic loop between helices VII and VIII<br />
of the Cab. thermophilum. The positions of the FX Cys ligands and the conserved histidine ligand<br />
to the reaction center special pair (P700, P840, P780) are indicated. The antenna (helices I to VI)<br />
and electron-transfer domains (helices VII to XI) of the polypeptides are indicated.<br />
Abbreviations and sequence alignments of selected Type-1 reaction center proteins can be found<br />
in Figures 2S and 3S.<br />
Figure 2S. Sequence alignment of the N-terminal region of PscA of Cab. thermophilum and the<br />
homologous PscA, PshA, PsaA, and PsaB proteins of the Type-1 reaction centers and other<br />
photosynthetic bacteria. The region shown corresponds to that including transmembrane α–<br />
helices I through VII, including all of the antenna domain and the first transmembrane helix of<br />
the electron transfer domain; the positions of these helices <strong>for</strong> Thermosynechococcus elongatus,<br />
as derived from the X-ray structure <strong>for</strong> photosystem I (47), are indicated. Note that the long<br />
inserted loop sequence that follows helix VII of Cab. thermophilum has been truncated, since it<br />
is not related in sequence to any portion of any other reaction center protein. Hyphens indicate<br />
insertions/deletions included to optimize the alignment. Abbreviations: Cab.,<br />
Chloracidobacterium; Chl., Chlorobium; Hbc., Heliobacillus; Hbt., Heliobacterium; Pld.,<br />
Pelodictyon; Ptc., Prosthecochloris; T., Thermosynechococcus.<br />
13
Figure 3S. Sequence alignment of the C-terminal region of PscA and the homologous PshA,<br />
PsaA, and PsaB proteins of the reaction centers of Cab. thermophilum and other photosynthetic<br />
bacteria. The region shown corresponds to transmembrane α–helices VIII through XI, including<br />
most of the electron-transfer cofactor binding domain; the positions of these helices <strong>for</strong><br />
Thermosynechococcus elongatus as derived from the X-ray structure <strong>for</strong> photosystem I (47) are<br />
shown. Hyphens indicate insertions/deletions included to optimize the alignment. The position of<br />
the conserved, cysteine-containing loop (red boxes enclose the conserved cysteine residues) that<br />
is predicted to provide the ligands to an intersubunit FX-like, [4Fe-4S] cluster is indicated by the<br />
bar labeled FX. The conserved His in Helix X that is the putative ligand to one BChl/Chl of the<br />
reaction center special pair is also indicated by a red box. Abbreviations are the same as in<br />
Figure 3S.<br />
Figure 4S. Sequence alignment of PscB sequences from various organisms. Hyphens indicate<br />
insertions/deletions included to optimize the alignment. Abbreviations: Cab.,<br />
Chloracidobacterium; Chl., Chlorobium; Ptc., Prosthecochloris. The eight conserved cysteines<br />
that are likely to be the ligands to two [4Fe-4S] clusters are indicated in red boxes and in the<br />
consensus sequence.<br />
Figure 5S. Sequence alignment of selected FmoA proteins, the apoprotein of the BChl a-binding<br />
Fenna-Matthews-Olson protein. Hyphens indicate insertions/deletions included to optimize the<br />
alignment. Abbreviations: Cab., Chloracidobacterium; Chl., Chlorobium; and Ptc.,<br />
Prosthecochloris.<br />
14
Figure 6S. Physical map of a 950-kbp region of the consensus Cab. thermophilum genome,<br />
including BAC clone M60-018 J19 and showing the relative positions of the rRNA operon, recA,<br />
pscA-pscB-fmoA, bchG-orf-csmA, and bchY genes. A. Physical map of assembled scaffolds and<br />
contigs from the Mushroom Spring metagenome (dark blue bars) mapped onto the sequence of<br />
BAC clone M60-018 J19 (blue-green bar). This BAC clone is completely sequenced and<br />
comprises 271,846 bp (GenBank accession EF531339). The colored boxes indicate the positions<br />
of the 16S rRNA (purple), recA (red), and pscAB-fmoA (green) genes. B. Extension of the map in<br />
Panel A that shows the physical map of sequenced BAC clone M60-018 J19 (blue-green bar) and<br />
other BAC clones (gray bars) whose end-sequences (pink boxes) were mapped onto the scaffolds<br />
and contigs (dark blue bars) assembled from the Mushroom and Octopus Springs metagenome.<br />
The positions of the bchG-orf-csmA (yellow-green) and bchY (orange) genes are indicated,<br />
establishing that these genes are also physically linked to the 16S rRNA gene in BAC clone<br />
M60-018-J19.<br />
Figure 7S. Comparison of the complete sequence of the 16S rRNA of Cab. thermophilum<br />
derived from BAC clone M60-018 J19 with the nearly complete sequence from Acidobacterium<br />
GFP1 (37). The red ellipses indicate the differences between the two sequences.<br />
Figure 8S. Rooted neighbor-joining phylogram based upon the sequence alignment shown in<br />
Figure 9S, which shows the relationships of RecA sequences of Cab. thermophilum and various<br />
other organisms. The RadA-like protein (DR1105) of Deinococcus radiodurans was used as the<br />
outgroup sequence. Some of the major eubacterial phyla are listed at the right.<br />
Chlorophototrophs and the phyla that contain them are shown in colors, and all other phyla and<br />
15
organisms are indicated in black. Bootstrap values were determined on the basis of 2000<br />
resamplings.<br />
Figure 9S. Alignment of the RecA sequences from the indicated organisms that was used to<br />
generate the tree shown in Figure 8S. Hypens indicate insertions/delections included to optimize<br />
the alignment. Abbreviations: Cab., Chloracidobacterium; Car., Carboxydothermus; Cfx.,<br />
Chloroflexus; Hb., Heliobacterium; Pel., Pelotomaculum; Pld., Pelodictyon; The.,<br />
Thermoanaerobacter.<br />
Figure 10S. Rooted neighbor-joining phylogram derived from the sequence alignment shown in<br />
Figure 11S showing the relationship of the BchU sequence of Cab. thermophilum to the BchU<br />
and CrtF sequences of various organisms. The ComT methyltransferase of Ara. thaliana was<br />
used as the outgroup. Abbreviations: A., Arabidopsis; Cab., Chloracidobacterium; Cfx.,<br />
Chloroflexus; Chl., Chlorobium; Pld., Pelodictyon; Ptc., Prosthecochloris; Rhb., Rhodobacter;<br />
Rps., Rhodopseudomonas; Rvx., Rubrivivax. The bootstrap values shown are based on 1000<br />
resamplings.<br />
Figure 11S. Alignment of BchU, CrtF, and ComT sequences from the indicated organisms that<br />
was used to generate the tree shown in Figure 10S. Hyphens indicate insertions/deletions<br />
included to optimize the alignment. Abbreviations: A., Arabidopsis; Cab., Chloroacidobacterium;<br />
Cfx., Chloroflexus; Chl., Chlorobium; Pld., Pelodictyon; Ptc., Prosthecochloris; Rhb.,<br />
Rhodobacter; Rps., Rhodopseudomonas; Rvx., Rubrivivax.<br />
16
Figure 12S. Alignment of sequences <strong>for</strong> AcsF, the oxygen-dependent oxidative ring cyclase <strong>for</strong><br />
Mg-protoporphyrin IX monomethyl ester in BChl and Chl biosynthesis (41, 43, 44). Hyphens<br />
indicate insertions/deletions included to optimize the alignment. Abbreviations: A., Arabidopsis;<br />
Cab., Chloracidobacterium; Cfx., Chloroflexus; G., Gloeobacter; Por., Porphyra; Rb.,<br />
Rhodobacter; Rfx., Roseiflexus; Rps., Rhodopseudomonas.<br />
Figure 13S. Complete HPLC elution profile monitored at 667 nm <strong>for</strong> pigments extracted from<br />
an enrichment culture containing Synechococcus sp. strain JA-2-3B'a (2-13), Cab. thermophilum,<br />
and Anoxybacillus sp. The blue-colored block indicates the elution positions of BChl c homologs<br />
esterified with farnesyl (indicated by the subscript “F”). The yellow colored block indicates the<br />
elution position of BChl c homologs esterified with geranylgeraniol (indicated by the subscript<br />
“GG”). Most of the BChl c is esterified with alcohols more hydrophobic than geranylgeraniol<br />
(aqua colored block), and at least two distinct alcohol groups appear to be present because of the<br />
complexity of the pattern observed. The peaks numbered 1 to 4 indicate the typical pattern of<br />
[ethyl, methyl], [ethyl, ethyl], [propyl, ethyl] and [i-butyl, ethyl] BChl c homologs, and the<br />
letters “a” and “b” indicate peaks probably derived from two different esterifying alcohol groups.<br />
Peaks from Chl a of Synechococcus sp. strain JA-2-3B'a (2-13) are indicated by the two arrows.<br />
The inset shows the spectrum of peak 3b, which is characteristic of BChl c under these solvent<br />
conditions.<br />
17
Figure 1S<br />
Antenna domain<br />
Synechococcus sp. 7002 PsaA<br />
Synechococcus sp. 7002 PsaB<br />
Chl. tepidum PscA<br />
Hbt. modesticaldum PshA<br />
Cab. thermophilum PscA<br />
Electron transfer domain<br />
F X<br />
F X<br />
F X<br />
F X<br />
F X<br />
P840<br />
= Transmembrane alpha-helix<br />
P700<br />
P780<br />
P700<br />
P840<br />
739 aa<br />
733 aa<br />
731 aa<br />
608 aa<br />
865 aa
PscA/PshA/PsaA/PsaB/ N-terminal Alignments<br />
T. elongatus PsaB<br />
Synechococcus 7002 PsaB<br />
T. elongatus PsaA<br />
Synechococcus 7002 PsaA<br />
Hbc. mobilis PshA<br />
Hbt. gestii PshA<br />
Hbt. modesticaldum PshA<br />
Cab. thermophilum PscA<br />
Ptc. aestuarii PscA<br />
Pld. luteolum PscA<br />
Chl. tepidum PscA<br />
Chl. phaeobacteroides 266 PscA<br />
Chl. phaeo. PscA<br />
Chl. limicola PscA<br />
Chl. chlorochromatii PscA<br />
Chl. vibrio<strong>for</strong>me PscA<br />
Chl. phaeo BS1 PscA<br />
Chl. limicola thio. PscA<br />
10 20 30 40 50 60 70 80 90 100 110 120<br />
M A T K F P K F S Q D L A Q D P - T T R R I W Y A I A M A H D F E S H D G M T E E N L Y Q K I F A S H F G H L A I I F L W V S G S L F H V A W Q G N F E Q W V Q<br />
M A T K F P K F S Q D L A Q D P - T T R R I W Y G I A T A H D F E T H D G M T E E N L Y Q K I F A S H F G H L A I I F L W T S G T L F H V A W Q G N F E Q W I K<br />
M P S F L P C L R E R R D S M T I S P P E R E P K V R V V V D N D P V P T S F E K W A K P G H F D R T L A R G P Q T T T W I W N L H A L A H D F D T H T - S D L E D I S R K I F S A H F G H L A V V F I W L S G M Y F H G A K F S N Y E A W L A<br />
M A V K V D K D P V P T S F E K W G K P G H F D R T L A R G P K T T T W I W N L H A D A H D F D S Q T - S D L E D I S R K I F S A H F G H L A V V F V W L S G M Y F H G A K F S N Y E A W L N<br />
M A T A D A A F N P - - - - - - - - - - - R A Q V F E W F K - D K V P A T R G A V L K A H I N H L G M V A G F V S F V L V H - - - - - - - - - - - -<br />
M A T A E M A F N P - - - - - - - - - - - R A Q V F E Y F K - D K V P A T R G A V L K A H I N H L G N I S A M V S F I L V H - - - - - - - - - - - -<br />
M A T A E M A F N P - - - - - - - - - - - R A Q V F E Y F K - D K V P A T R G A V L K A H I N H L G N V A A M V S F I L V H - - - - - - - - - - - -<br />
M A S F S S - - - - - - - - - - Y A N G V K R W Y Q K L E L P M P P E R I F G A H M M L I G G L A C L I G T Y F F A S M T M W N - D G Y V N<br />
M A D Q A N P T G V M P K G K V P P P K G K V P P P K G N - G S P G S A S V V Q E K D A A K M K R F L F Q R T E T R S T K W Y Q I F D T D K L<br />
M A E Q V N P A G V K P K G T V P P P K G S A P S P K A S - G A S G G P S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q V F D T D K I<br />
M A E Q V K P A G V K P K G T V P P P K G N A P A P K A N - G A P G G A S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T E K L<br />
M A E Q V N P A G V K P K G S V P P P K G N A P S P K G N - G A S G G P S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T E K L<br />
M A D Q A N P T G V K P K G Q V P P P K G K V P P P K G N G A P A G G A S I I K E K D A A K M R R F L F Q R T E T R S T K W Y Q V F D T D K V<br />
M A E Q V N P A G V K P K G S V P P P K G N A P S P K G N - G A P G G P S V I K E Q D A A K M R R F L F Q R T E T R T T K W Y Q V F D T D K L<br />
M A E Q V N P A G V K P K G T V P P P K G N A P S P K G N - G A A G A P S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T D R V<br />
M A E Q V N P A G A V P K A K V P P P K G N A P S P K G D - G T S G A P S V I K E Q D A A K M R R F L F Q R T E T R T T K W Y Q I F D T D K I<br />
M A D Q A N P T G V K P K G Q V P P P K G K V P P P K G N G A P A G G A S I I K E K D A A K M R R F L F Q R T E T R S T K W Y Q V F D T D K V<br />
M A E Q V K P A G V K P K G T V P P P K G N A P A P K A N - G A P G G A S V I K E Q D A A K M R R F L F Q R T E T R S T K W Y Q I F D T E K I<br />
M P S F L P C L R E R R D S M T I S P P E R E P K V V D D P V P T S F E K F M A . Q . N P A G V K P K G V P P P K G . P P K G . G S V . K E . D A A K M . R F L F Q R T E T R . T K W Y Q . F D T . K .<br />
T. elongatus PsaB<br />
Synechococcus 7002 PsaB<br />
T. elongatus PsaA<br />
Synechococcus 7002 PsaA<br />
Hbc. mobilis PshA<br />
Hbt. gestii PshA<br />
Hbt. modesticaldum PshA<br />
Cab. thermophilum PscA<br />
Ptc. aestuarii PscA<br />
Pld. luteolum PscA<br />
Chl. tepidum PscA<br />
Chl. phaeobacteroides 266 PscA<br />
Chl. phaeo. PscA<br />
Chl. limicola PscA<br />
Chl. chlorochromatii PscA<br />
Chl. vibrio<strong>for</strong>me PscA<br />
Chl. phaeo BS1 PscA<br />
Chl. limicola thio. PscA<br />
130 140 150 160 170 180 190 200 210 220 230 240<br />
D P V N T R P I A H A I W D P Q F G K A A V D A F T Q A G A S N P V D I - - - - - A Y S G V Y H W W Y T I G M R T N G D L Y Q G A I F L L I L A S L A L F A G W L H L Q P K F R P S L S W F K N A E S R L N H H L A G - - L F G V S S L A W A G<br />
D P L N V R P I A H A I W D P H F G Q G A I D A F T Q A G A S N P V N I - - - - - A Y S G V Y H W F Y T I G M T T N A D L Y Q G A V F L L I L S S L F L F A G W L H L Q P K F R P S L A W F K D A E S R L N H H L A G - - L F G V S S L A W T G<br />
D P T G I K P S A Q V V W P I V G - - - Q G I L N G D V G G G F H G I Q - - - - - I T S G L F Q L W R A S G I T N E F Q L Y C T A I G G L V M A G L M L F A G W F H Y H - K R A P K L E W F Q N V E S M L N H H L A G - - L L G L G S L A W A G<br />
N P T V I K P S A Q V V W P V V G - - - Q G I L N G D V G G G F S G I Q - - - - - I T S G L F Y L W R A A G F T N S Y Q L Y V T A I G G L V M A A L M V F A G W F H Y H - K A A P K L E W F Q N A E A M M N H H L S V - - L L G C G S L G W T G<br />
- - - - - - - - - H L S W L S - - - - - - D Q V L F A P T P - - - - - - - - - - - - - - I F Y A R L Y Q L G L D A S - A R S A D A L M V A R L H L L A A I I F W I I G H - I K T P R E D E F L K N V S F G K T L V A Q F H F L A L V A T L W G M<br />
- - - - - - - - - H L S W D P A T - - - - Q G V L W A P A T - - - - - - - - - - - - - - M F Y A R L Y Q L G M D A T - A L S P D A L F V A R M H L L A A I I L W G V G H - I K S P A E E K F L E K V T M G K S L V A Q F H F F A L I A T L W G L<br />
- - - - - - - - - H L S W D P A T - - - - Q G V L W A P A T - - - - - - - - - - - - - - M F Y A R L Y Q L G L D A T - A L S P D A L F V A R M H L L A A I I L W G F G H - V K S P A E E K F L E K V T M G K A L V A Q F H F F A L I A T L W G L<br />
L T L R P R L I A L G I Y D P Y D T E Q I Q R V W L P L I G E F S T S - - - - - - - K L P F F G Q Y P L T M T D F R L F G W G C F H I G L G L W L V Y A G A A H Y Y G A R G G A T I G E I F W L L P Y V P G L K G L C Q I K W F T P D G P W Y K<br />
D D E Q V V G - A H L A L L G V L G F V M G I Y Y L S G M Q V M P W G - - - A P G F H D N W F Y L T I K P R M V S L G I D T Y S P K T E D L M A A S T K L M G W A I L H - F V S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F R<br />
D D E Q V V G - G H L A L L G V L G F L M A I Y Y F S G I Q V F P A W L G G M P G F H D N W F Y L T I K P R M V S L G I D T Y S T Q T A D L Q Q A A T N L I G W A A F H - F L S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F R<br />
D D E Q V V G - G H L A L L G V L G F I M G I Y Y I S G I Q V F P W G A P G - - - F H D N W F Y L T I K P R M V S L G I D T Y S T K T A D L E A A G A R L L G W A A F H - F L V G S V L I F G - G W R H W T H N L T N P F T G R C G N F R D F R<br />
D D E Q V V G - G H L A L L G V M G F L M A I Y Y I S G I Q V F P W G A P G - - - F H D N W F Y L T I K P R M V S M G I D T Y S T R S A D L E Q A A T N L L G W A L L H - V I S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F R<br />
D D E Q V V G - G H L A L L G V L G F I M G I Y Y L S G M Q V L P W N G - - A P A F H D N W F Y L T I K P R M V S L G I D T Y S P R T E D L I A A S W K L M G W A I L H - F V S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R E F R<br />
D D E Q V V G - G H L A L L G V M G F L M A I Y Y I S G I Q V F P G G Y A G - - - F H D N W F Y L T I K P R M V S L G I D T Y S T K T A D L E Q A A I N L L G W A L L H - V I S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F R<br />
D D E Q V V G - A H L A L L G F L G Y L M A I Y Y I S G V Q V F P W G A P G - - - F Q D N W F Y L T I K P R M V S L G I D T Y S T K T E D L Q W A A S N L L G W A L F H - I I S G S I L I F G - G W R H W T H N L T N P F T G R A G N F R D F R<br />
D D E Q V V G - G H L A L L G V L G F L M A I Y Y I S G I Q V F P A F L G G G P G F H D N W F Y L T I K P R M V S L G I D T Y S T N T A D L Q Q A A T N L L G W A A F H - F L S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R D F R<br />
D D E Q V V G - G H L A L L G V L G F I M G I Y Y L S G M Q V L P W N G - - A P A F H D N W F Y L T I K P R M V S L G I D T Y S P R T E D L I A A S W K L M G W A I L H - F V S G S I L L F G - G W R H W T H N L T N P F T G R A G N F R E F R<br />
D D E Q V V G - G H L A L L G V L G F I M A I Y Y I S G I Q V F P W G A P G - - - F H D N W F Y L T I K P R M V S L G I D T Y S T K T A D L E W A G T K L L L W A G Y H - F I A G S V L I F G - G W R H W T H N L T N P F T G R C G N F R D F R<br />
D D E Q V V G I G H L A L L G V . G F . M . I Y Y . S G . Q V F P . P G A P G F H D N W F Y L T I K P R M V S G I D T Y S . . D L A . L . G W A . . H P . S G S . L . F G G W R H W T H N L T N P F T G R . G N F R . F R<br />
T. elongatus PsaB<br />
Synechococcus 7002 PsaB<br />
T. elongatus PsaA<br />
Synechococcus 7002 PsaA<br />
Hbc. mobilis PshA<br />
Hbt. gestii PshA<br />
Hbt. modesticaldum PshA<br />
Cab. thermophilum PscA<br />
Ptc. aestuarii PscA<br />
Pld. luteolum PscA<br />
Chl. tepidum PscA<br />
Chl. phaeobacteroides 266 PscA<br />
Chl. phaeo. PscA<br />
Chl. limicola PscA<br />
Chl. chlorochromatii PscA<br />
Chl. vibrio<strong>for</strong>me PscA<br />
Chl. phaeo BS1 PscA<br />
Chl. limicola thio. PscA<br />
250 260 270 280 290 300 310 320 330 340 350 360<br />
H L I H V A I P E S R G - Q H V G W D N F L S T M P H P A G L A P F F T G N W G V Y A Q - - N P D T A S H V F G T A Q G A G T A I L T F L G G F H P Q T E S L W L T - D M A H H H L A I A V L F I V A G H M Y R T Q F G I G H S I K E M M D A K<br />
H L V H V A I P E S R G - V H V G W D N F L S V K P H P A G L M P F F T G N W G V Y A Q - - N P D T A G H V F G T S Q G A G T A I L T F L G G F H P Q T E S L W L T - D I A H H H L A I A V L F I I A G H M Y R T N V R I G H S I K E I L N A H<br />
H Q I H V S L P I N K L - L D A G V A A K D I P L P H E F I L N P S L M A E L Y P K V D W G F F S G V I P F F T F N W A A Y S D F L T F N G G L N P V T G G L W L S - D T A H H H L A I A V L F I I A G H M Y R T N W G I G H S L K E I L E A H<br />
H L I H V S L P V N K L - L D S G V A A K D I P L P H E F F D - A S V M A E L Y P - - - - S F A Q G L K P F F T L D W A A Y S D F L T F K G G L N P T T G S L W L S - D V A H H H L A I A V L F I I A G H M Y R T G F G I G H S M K E I L E A H<br />
H M A Y I G V R G A N G G I V P T G L S F D M F G P I T G A T L A G N H V A F G A L L - - - F L G G V F H H F A - - - G F N T K R F A F F E K D W E A V L S V S A Q - V L A F H F A T V V F A M I I W N R P D Q P I L S F Y F M Q D Y A L S N Y<br />
H M A F Y G I L G P E G K L A P T G L S F D M F G P I T P A S M A G N H V A F G A V F - - - F L G G I F H Y F A - - - G F N T K K F A F F E K D W E A V L S V S C Q - V L A F H F A T V V F A M I I W Q H P - - - Q L G F G F M R E Y A V S Q Y<br />
H M A F Y G I L G P S G K L E P T G L S F D M F G P I T P A T M A G N H V A F G A V F - - - F L G G I F H Y F A - - - G F N T K R F A F F E K D W E A V L S V S C Q - I L A F H F A T V V F A M I I W Q H P - - - Q L G F G F M R E Y A V S Q Y<br />
V G L P W G S F A N T P W R I L R R T Y A D A L G A H T I Y I G L L F - F I W G F V L W F V L D K P P V P L Q P - - - A Q V M T P N G P M P I E Q A P F P Y G W F D P Y L N E V M H P M N T I N G E T T M S F V W G V L F V V L G A Y W W Y R P<br />
F L G K F G D A V F Q G - - T S A K T Y K D A L G P H T V Y M A L L F - L G W G F M L W G V L G I A P I P D F Q T S N S E T F M S F V W F V V F F A L G L Y W W K N P P N A A T H L N D D M K A A F S V H L T A I G Y V N I A L G I I A F V A F<br />
F L G K F G D M V F N G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L L M W F V L G F A P V P D F Q T I N S E T F M S F V W F V A F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A L G V I A F V A F<br />
F L G K F G D V V F N G - - T S A K S Y K E A L G P H A V Y M S L L F - L G W G I V M W A I L G F A P I P D F Q T I N S E T F M S F V F A V I F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A L G C I A F V A F<br />
F L G K F G D V V F S G - - T S A K S Y K D A L G P H A V Y M S L L F - L G W G L I M W L A L G W A P V P D F Q T I N S E T F M S F V W F V V F F A L G I Y W W N N P P N A A T H L N D D M K A A F S V H L T A I G Y I N I A L G V I A F V A F<br />
F L G K F G D A V F Q G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L M L W I V L G F A P I P D F Q T I N S E V F M S F V W F L V F F A L G L Y W W K N P P N A A I H L N D D M K A A F S V H L T A I G Y V N I A L G I I A F V A F<br />
F L G K F G D M A F N G - - T S A K T Y K D A L G P H T V Y M S L L F - L G W G L V M W F A L G I P P V P D F Q T I N S E P F M S F V F A V L F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A L G V I A F V A F<br />
F L G K F G D V V F S G - - T S A K S Y K D A L G P H T V Y M S L L F - L G W G F V M W F V L G F A P I P D F Q T I N S E T F M S F I F A V I F F A A G I Y W W N N P P N A A T H L N D D M K A A F S V H L T A I G Y I N I A L G C I A F V A F<br />
F L G K F G D A V F N G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L L M W L V L G Y A P V P D F Q T I N S E T F M S F V W F A V F F A L G L Y W W N N P P N A A I H L N D D M K A A F S V H L T A I G Y I N I A I G V I A F V A F<br />
F L G K F G D A V F Q G - - T S A K T Y K D A L G P H A V Y M S L L F - L G W G L M L W I V L G F A P I P D F Q T I N S E V F M S F V W F L V F F A L G L Y W W K N P P N A A I H L N D D M K A A F S V H L T A I G Y V N I A L G I I A F V A F<br />
F L G K F G D M V F S G - - T S A K S Y K E A L G P H A V Y M S L L F - L G W G L V M W L I L G F A P I P D F Q T I N S E T F M S F V F A V I F F A L G I Y W W N N P P N A A I H L N D D M K A A F S V H L T H R - L H Q H R A W V I A F V A F<br />
F L G K F G D V F G T S A K . Y K D A L G P H V Y M S L L F L G W G . . W V L G . P . P D F Q T I N S E F M S F . F . . F F A . G . Y W W N P P N A A H L N D D M K A A F S V H L T A I G . . . . . . G I A F V A F<br />
T. elongatus PsaB<br />
Synechococcus 7002 PsaB<br />
T. elongatus PsaA<br />
Synechococcus 7002 PsaA<br />
Hbc. mobilis PshA<br />
Hbt. gestii PshA<br />
Hbt. modesticaldum PshA<br />
Cab. thermophilum PscA<br />
Ptc. aestuarii PscA<br />
Pld. luteolum PscA<br />
Chl. tepidum PscA<br />
Chl. phaeobacteroides 266 PscA<br />
Chl. phaeo. PscA<br />
Chl. limicola PscA<br />
Chl. chlorochromatii PscA<br />
Chl. vibrio<strong>for</strong>me PscA<br />
Chl. phaeo BS1 PscA<br />
Chl. limicola thio. PscA<br />
370 380 390 400 410 420 430 440 450 460 470 480<br />
D F F G T K V E G P F N M P H Q G I Y E T Y N N S L H F Q L G W H L A C L G V I T - - - S L V A Q H M Y S - L P P - - Y A F I A Q D H T T M A A L Y T H H Q Y I A G F L M V G A F A H G A I F L V R D Y D P A Q N K G N V L D R V L Q H K E A I<br />
Q P P - - - - S G K L G A G H K G L Y D T V N N S L H F Q L G L A L A S L G V I T - - - S L V A Q H M Y S - L P S - - Y A F I A K D Y T T Q A A L Y T H H Q Y I A G F L M V G A F A H G A I F F V R D Y D P E A N K D N V L Y R M L E H K E A L<br />
K G P - - - - - - F T G A G H K G L Y E V L T T S W H A Q L A I N L A M M G S L S - - - I I V A Q H M Y A - M P P - - Y P Y L A T D Y P T Q L S L F T H H M W I G G F L V V G G A A H G A I F M V R D Y D P A M N Q N N V L D R V L R H R D A I<br />
K G P - - - - - - F T G E G H K G L Y E I L T T S W H A Q L A V N L A L L G S L T - - - I V I A H H M Y S - M P P - - Y P Y M A T D Y G T Q L S L F T H H T W I G G F L I V G A G A H G A I F M V R D Y D P A K N V N N L L D R V I R H R D A I<br />
A A P - - - E I R E I A S Q N P G F L I K Q V I L G H L V F G V M F W I G G V F H G - - A H F M L R A T N - D P K - - L A E A L K D F K M L K R C Y D H D F Q K K F L A L I M F G A F L P I F V S Y G - - - I A T H N T I A D L H H L A K A G M<br />
A G P - - - E L K M I A Q G N P G L L V K Q A I L G H L V M G I M F W I G G V F H G - - A H F M L R V L N - D P K - - M A E E M K D F K F I K R C Y D H E F Q K K F L A L I M F G A F L P I F V A Y G - - - I A T H N T I A D I H A A A K T G I<br />
A G P - - - E L K M I A Q S N P G L L V K Q A I L G H L V M G I M F W I G G V F H G - - A H F M L R V L N - D P K - - L A E E M K D F K F I K R C Y D H E F Q K K F L A L I M F G A F L P I F V S Y G - - - I A T H N T I A D I H A A S K T G L<br />
P R S - - I N I T H L E D T K A V F H V H L T S I G Y V S F A L A I V G F L A L R N H P S Y L I L N D M N V I I Y G K K I V N P G R M I H N M I T F N H V Q V G L L Y V A A G V F H G G Q Y L H G L N I S G A Y K Q A R S K F I T W F Q N P D L<br />
Q Q D - S F A P Y Y A E L D K L V F Y I Y G E P F N R V S F D F V Q E G G K V I A G S K E F E E F A A Y A I L P K N G E S F G M A R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q I N G L Y S Q I K S I W V A K G R D Q D L<br />
Q Q P - S F A P Y Y K E L D K L V F Y L Y G E P F N R V S Y D F V Q E G G R I V S G S K E F S E F A P F A I L P K N G A S F G M S R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R N Q E V<br />
Q Q P - S F A P Y Y K E L D K L V F Y L Y G E P F N R V S F N F V E Q G G K V I S G A K E F A D F P A Y A I L P K S G E A F G M A R V V T N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G M Y N Q I K S I W I T K G R D Q E V<br />
Q Q P - S F A P Y Y K E L D N L V F Y L Y G E P F N R V S Y D F V Q E G G Q I V S G S K E F T E F A P Y A I L P K N G E A F G M S R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R D Q E V<br />
Q Q D - S F A P Y Y A E L D K L V F Y I Y G E P F N R V S F D F V E Q G G K V L S G S K E F A E F P A F A I L P K N G E S F G M A R V V I N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K V Q I N G L Y S Q I K S I W V A K G R D Q E L<br />
Q Q P - S F H A Y Y K E L D N L V F Y L Y G E P F N R V S Y D F V Q E G G R I I S G K K E F V E F A P Y A I L P K N G Q A F G M S R V V I N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R D Q E V<br />
Q Q P - S F A D Y Y K A L D S L V F Y I Y G E P F N R V S Y D Y V E A G G R I V S G S K E F A D F P A Y A I L P K D G A A F G M S R T V I N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y S Q I K S V F I T K G R D Q E V<br />
Q Q P - S F A P Y Y Q E L D N L V F Y L Y G E P F N R V S Y D F V Q E G G R I V S G S K E F A E F A P F A I L P K N G Q S F G M S R V V I N L I S F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G L Y N Q I K S I W I T K G R D Q E V<br />
Q Q D - S F A P Y Y A E L D K L V F Y I Y G E P F N R V S F D F V E Q G G K V L S G S K E F A E F P A F A I L P K N G E S F G M A R V V I N L I V F N H I I C G V L Y V F A G V Y H G G Q Y L L K V Q I N G L Y S Q I K S I W V A K G R D Q E L<br />
Q Q P - S F A P Y Y K E L D K L V F Y I Y G E P F N R V S F N F V E Q G G K V I S G A K E F A E F P A Y A I L P R N G E S F G M A R V V T N L I T F N H I I C G V L Y V F A G V Y H G G Q Y L L K I Q L N G I Y N Q I K S I W I T K G R D Q E V<br />
Q Q P G S F A Y Y E L D . L V F Y . Y G E P F N R V S . . V G G V . S G S K E F . . F Y A I L P K N G . F G M R V N L I F N H I I C G V L Y V F A G V Y H G G Q Y L L K . Q . N G . Y N Q I K S . . I K G R Q . .<br />
T. elongatus PsaB<br />
Synechococcus 7002 PsaB<br />
T. elongatus PsaA<br />
Synechococcus 7002 PsaA<br />
Hbc. mobilis PshA<br />
Hbt. gestii PshA<br />
Hbt. modesticaldum PshA<br />
Cab. thermophilum PscA<br />
Ptc. aestuarii PscA<br />
Pld. luteolum PscA<br />
Chl. tepidum PscA<br />
Chl. phaeobacteroides 266 PscA<br />
Chl. phaeo. PscA<br />
Chl. limicola PscA<br />
Chl. chlorochromatii PscA<br />
Chl. vibrio<strong>for</strong>me PscA<br />
Chl. phaeo BS1 PscA<br />
Chl. limicola thio. PscA<br />
490 500 510 520 530 540 550 560 570 580 590 600<br />
I S H L S W V S L F L G F H T L G L Y V H N D V V V A F G T P E K Q I L I E P V F A Q F I Q A A H G K L L Y G F D T L L S N P D S I A S T A W P N Y G N V W L P G W L D A I N S G T N S L F L T<br />
I S H L S W V S L F L G F H T L G L Y V H N D V V V A F G T P E K Q I L I E P V F A Q F V Q A A S G K A L Y G F D V L L S N A D S I A S N A S A A Y - - - - L P G W L D A I N S G S N S L F L N<br />
I S H L N W V C I F L G F H S F G L Y V H N D T M R A F G R P Q D M F S D T G I Q L Q P V F A Q W V Q N L H T L A P G G T A P N A A A T A S V A F G G D V V A V G G K V A M M P I V<br />
I S H L N W V C I F L G F H S F G L Y I H N D T M R A L G R P Q D M F S D S A I Q L Q P I F A Q W I Q N I H T L A P G G T A P N A I A S A S Q V F G G D V V A I G N K V A L M P I T<br />
F A N M T Y I N I G T P L H D A I F G S H G<br />
F A H M T Y I N I G T P L H D A V F G S K G<br />
F A H M T Y I N I G T P L H D A I F G S K G<br />
Q T K I V G T T M F V S F V T V V F - G Y G M I C W N T G A E L D L N F G I Y Q F R S F R A I Q M D G E A G N I G Y R V F R P N N P W D P T A G G D W V K N P D G T A K L V K A R N L Q V G D R I L N E E L G I G S S P T Y S F T T<br />
Q V K V L G T I M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P F L H W M F E D P<br />
Q V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P M F Q W M F A D P<br />
Q V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I T M S F Y W L K P L P I F Q W M F A D P<br />
Q V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P M F Q W M F A D P<br />
Q V K I L G T I M A L C F A T M L S - V Y A V I V W N T I C E L N F F G T N - - - - I M M S F Y W L K P L P F L H W M F E D P<br />
Q V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P I F Q W M F T D P<br />
Q V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N L F G T N - - - - I M M S F Y W L K P L P M F Q W M F N D P<br />
Q V K I L G T V M A L C F A T M L S - V Y A V I V W N T I C E L N I F G T N - - - - I M M S F Y W L K P L P M F Q W M F A D P<br />
Q V K I L G T I M A L C F A T M L S - V Y A V I V W N T I C E L N F F G T N - - - - I M M S F Y W L K P L P F L H W M F E D P<br />
Q V K I L G T V M A L C F S T M L S - V Y A V I V W N T I C E L N L F G T N - - - - I T M S F Y W L K P L P I F Q W M F A D P<br />
Q V K I L G T . M A L C F . T M L S Y V Y A V I V W N T I C E L N F G T N F I M M S F Y W L K P L P F W M F D P N I A S G V G A N S L F L I L N E E L G I G S S P T Y S F T T<br />
I<br />
II<br />
III IV<br />
V VI<br />
VII<br />
Figure 2S
Figure 3S<br />
PscA/PshA/PsaA/PsaB C-terminal Alignments<br />
T. elongatus PsaB<br />
Synechococcus 7002 PsaB<br />
T. elongatus PsaA<br />
Synechococcus 7002 PsaA<br />
Hbc. mobilis PshA<br />
Hbt. gestii PshA<br />
Hbt. modesticaldum PshA<br />
Cab. thermophilum PscA<br />
Ptc. aestuarii PscA<br />
Pld. luteolum PscA<br />
Chl. tepidum PscA<br />
Chl. phaeobacteroides 266 PscA<br />
Pld. phaeo. PscA<br />
Chl. limicola PscA<br />
Chl. chlorochromatii PscA<br />
Chl. vibrio<strong>for</strong>me PscA<br />
Chl. phaeobacteroides BS1 PscA<br />
Chl. limicola thio. PscA<br />
10 20 30 40 50 60 70 80 90 100<br />
I G P G D F L V H H A I A L G L H T T T L I L V K G A L D A R G S K L M P D - - - K K D F G Y A F P C D G P G R G G T C D I S A W D A F Y L A M F W M L N T I G W V T F Y W H W K H L G V W E G N V A Q<br />
I G P G D F L V H H A I A L G L H T T T L I L V K G A L D A R G S K L M P D - - - K K D F G Y S F P C D G P G R G G T C D I S A W D A F Y L A M F W M L N T L G W L T F Y W H W K H L G I W Q G N V A Q<br />
L G T A D F M V H H I H A F T I H V T V L I L L K G V L F A R S S R L I P D - - - K A N L G F R F P C D G P G R G G T C Q V S G W D H V F L G L F W M Y N C I S V V I F H F S W K M Q S D V W G T V A P<br />
L G T A D F M V H H I H A F T I H V T V L I L L K G L L Y S R S S R L V P D - - - K G Q L G F R F P C D G P G R G G T C Q V S G W D H V F L G L F W M Y N S L S I V I F H F S W K M Q S D V W G T I L P<br />
T V S D F V A A H A I A G G L H F T M V P L W R M V F F S K V S P W T T K V G M K A K R D G E F P C L G P A Y G G T C S I S L V D Q F Y L A I F F S L Q V I A P A W F Y L D G C W M G S F V A T S S E<br />
S I S E F V A A H A I A G G L H F T M V P M W R M V F F S K V S P W T T K V G M K A K R D G E F P C L G P A Y G G T C S I S L V D Q F Y L A I F F S L Q V I A P A W F Y I D G C W M G S F V A V A Q P<br />
S I S E F V A A H A I A G G L H F T M V P M W R M V F F S K V S P W T T K V G M K A K R D G E F P C L G P A Y G G T C S I S L V D Q F Y L A I F F S L Q V I A P A W F Y I D G C W M G S F V A V A A P<br />
E I N D A I F F W L M I G G W L F G F I P L L R I A F H N Y Q S P W Y R D F E W R K Q S P - D F P C I G P V K G G T C G V S I Q D Q M W F C I L F S I K P L S A I A W Y L D G G W I A T M M A R G - -<br />
S I N D W V M A H V I V A G S L F S L I A L A R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -<br />
S I N D W V M V H V I V A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -<br />
S I N D W V M A H V I T A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P -<br />
S I N D W V M A H V I T A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -<br />
S I N D W V M V H V I V A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P -<br />
S I N D W V M A H V I T A G S L F S L I A L V R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -<br />
S I N D W V M V H V I T A G S L F S L I A L V R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P -<br />
S I N D W V M V H V I V A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -<br />
S I N D W V M A H V I V A G S L F S L I A L A R I A F F S H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G V K G L S A V C W Y I D G A W I A S M M Y G V P -<br />
S I N D W V M A H V I T A G S L F S L I A L V R I A F F A H T S P L W D D L G L K K N S Y - S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G I K G L S A V C W Y I D G A W I A S M M Y G V P -<br />
S I N D W V M . H V I . A G S L F S L I A L V R I A F F H T S P L W D D L G L K K N S Y S F P C L G P V Y G G T C G V S I Q D Q L W F A M L W G . K G L S A V C W Y I D G A W I A S M M Y G V P P<br />
T. elongatus PsaB<br />
Synechococcus 7002 PsaB<br />
T. elongatus PsaA<br />
Synechococcus 7002 PsaA<br />
Hbc. mobilis PshA<br />
Hbt. gestii PshA<br />
Hbt. modesticaldum PshA<br />
Cab. thermophilum PscA<br />
Ptc. aestuarii PscA<br />
Pld. luteolum PscA<br />
Chl. tepidum PscA<br />
Chl. phaeobacteroides 266 PscA<br />
Pld. phaeo. PscA<br />
Chl. limicola PscA<br />
Chl. chlorochromatii PscA<br />
Chl. vibrio<strong>for</strong>me PscA<br />
Chl. phaeobacteroides BS1 PscA<br />
Chl. limicola thio. PscA<br />
110 120 130 140 150 160 170 180 190 200<br />
F N - - - - - - - - - - - - - - E S S T Y L M G W L R D Y L W L N S S Q L I N G Y N P F G T N N L S V W A W M F L F G H L V W A T G F M F L I S W R G Y W Q E L I E T - - - - L V W A H E R T P L A N L<br />
F N - - - - - - - - - - - - - - E K S T Y L M G W F R D Y L W A N S A Q L I N G Y N P Y G V N N L S V W A W M F L F G H L V W A T G F M F L I S W R G Y W Q E L I E T - - - - I V W A H E R T P L A N I<br />
D G T V - - - S H I T G G N F A Q S A I T I N G W L R D F L W A Q A S Q V I G S Y G - - - - S A L S A Y G L L F L G A H F I W A F S L M F L F S G R G Y W Q E L I E S - - - - I V W A H N K L K V A P A<br />
D G S V - - - S H V T G G N F A T S A I T I N G W L R D F L W A Q S S Q V I N S Y G - - - - S A L S A Y G I M F L A G H F V F A F S L M F L F S G R G Y W Q E L I E S - - - - I V W A H N K L K L A P A<br />
V Y K Q A A E L F K A N P T W F S L H A V S N F T S E G Y F S Y I I S Q T T R M F K Y Y - - - - D G H L V Q A L L G A H F I W A F T F S M L F Q Y R G S R D E G A - - - - M V L K W A H E Q V G L G F A<br />
N N E I Y Q A A L A A Y N A H N P L H Q L S P L T N M G Y F S Y I I Q Q T T A M F S R Y - - - - D G H M I Q A L L G A H F I W A F T F S M L F Q Y R G S R D E G S - - - - M V L K W A H E Q V G V G F A<br />
Y N D I Y Q A A L A T F N S H N P L H Q L S P L T N M G Y F S Y I I Q Q T T A M F S R Y - - - - D G H M I Q A L L G A H F I W A F T F S M L F Q Y R G S R D E G A - - - - M V L K W A H E Q V G V G F A<br />
- - - - - - - - - - - - - N E A Y Y L T H N I S H T G G V F L Y M W N E T T W I W T - - - - - - D N H L T A M L L L G H L I W F V S F A L W F K D R G S R A E G G D I Q S R W V R L M G K R L G I K T L<br />
- - - - - - - - A A D A K S W D A V A G L S H H Y S A G I F Y Y F W T E T V T I F S - - - - - - S S H L S V I L M I G H L I W F I S F A V W F E D R G S R L E G A D I Q T R T V R W L G K K F L N R D V<br />
- - - - - - - - A A D A K A W D S V A G L A H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L G R D V<br />
- - - - - - - - A A D A K A W D S I A H L H H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V<br />
- - - - - - - - V A D A K A W D S V A G L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L I W F I S F A V W F E D R G S R L E G A D I Q T R T V R W L G K K F L N R D V<br />
- - - - - - - - A A D A K A W D S V A G L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V<br />
- - - - - - - - V A D A K A W D S V A G L A H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S V I L M I G H L I W F I S F A V W F E D R G S R L E G A D I Q T R T I R W F G K K F L N R D V<br />
- - - - - - - - A A D A K A W D S V A G L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S P H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V<br />
- - - - - - - - A A D A K A W D S V A G L A H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L G R D V<br />
- - - - - - - - A A D A K A W D A V A G L S H H Y S A G I F Y Y F W T E T V T I F S - - - - - - S S H L S V I L M I G H L V W F I S F A V W F E D R G S R L E G A D V Q T R T I R W L G K K F L N R D V<br />
- - - - - - - - A A D A K A W D S I A H L Q H H Y T S G I F Y Y F W T E T V T I F S - - - - - - S S H L S T I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W L G K K F L N R D V<br />
N Y Q A A A D A K A W D . . A . L H H Y . G I F Y Y F W T E T V T I F S Y G S S H L S I L M I G H L V W F I S F A V W F E D R G S R L E G A D I Q T R T I R W . G K K F L . R D V<br />
T. elongatus PsaB<br />
Synechococcus 7002 PsaB<br />
T. elongatus PsaA<br />
Synechococcus 7002 PsaA<br />
Hbc. mobilis PshA<br />
Hbt. gestii PshA<br />
Hbt. modesticaldum PshA<br />
Cab. thermophilum PscA<br />
Ptc. aestuarii PscA<br />
Pld. luteolum PscA<br />
Chl. tepidum PscA<br />
Chl. phaeobacteroides 266 PscA<br />
Pld. phaeo. PscA<br />
Chl. limicola PscA<br />
Chl. chlorochromatii PscA<br />
Chl. vibrio<strong>for</strong>me PscA<br />
Chl. phaeobacteroides BS1 PscA<br />
Chl. limicola thio. PscA<br />
210 220 230 240 250 260 270 280 290 300<br />
V R W K D K P V A L S I V Q A R L V G L A H F S V G Y I L T Y A A F L I A S T A A K F G<br />
V R W K D K P V A L S I V Q A R L V G L A H F T V G Y V L T Y A A F L I A S T S G K F G<br />
I Q P R - - - - A L S I I Q G R A V G V A H Y L L G G I A T T W A F F L A R I I S V G<br />
I Q P R - - - - A L S I V Q G R A V G V A H Y L L G G I V T T W A F F L A R S L S I G<br />
G K V Y - - N R A L S L K E G K A I G T F L F F K M T V L C M W C L A M V<br />
G K V Y - - N R A L S L K E G K A I G T F L F F K M T I V C M W C L A M V<br />
G K M Y - - N R A L S L K E G K A I G C F L F F K M T I V C M W A L A M V<br />
Q E V R - - F P V S N L A T A K L W G T V F F Y T G T F V L V F L Y F A D G F F Q N R<br />
T F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N T P A L P P V G D A A I S G Q Q L L T Q V V D F I V K L I A<br />
N F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F M A N G F Y Q T D S P L P P T V S D A A V S G Q V L L T Q V V D Y L M K L I A<br />
N F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F L A N G F Y Q T N S P L P P P V S H A A V S G Q Q M L A Q L V D T L M K M I A<br />
T F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N S P L P P T V G D A S L S G Q Q M L T Q V V D Y L M K L I A<br />
T F R - - - F P V L T I S D S K M A G T F L Y F S G V F M L V F L F I G N G F Y Q T D S P L P P Q V G D A S V S G Q V M L T Q V V D Y L M K L I A<br />
T F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I G N G F Y Q T D S P L P P P V A E A A V S G Q Q M L T Q V V D F L I K L V A<br />
T F R - - - F P V L T I S D S K M A G T F L Y F G G V F M L V F L F I G N G F Y Q T D S P L P P Q V G D A S V S G Q M M L T Q V V D Y L L K L I A<br />
Q F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F L A N G F Y Q T D S P L P P T V S D A A V S G Q A L L T Q V V D Y L M K L I A<br />
Q F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N T P A L P P V G D A A V S G Q Q L L T Q V V D F I M K L I A<br />
N F R - - - F P V L T I S D S K L A G T F L Y F G G T F M L V F L F I A N G F Y Q T N S P L P P P V S H A A V S G Q Q M L A Q L V D T L M K M I A<br />
F R D K F P V L T I S D S K L A G T F L Y F . G T F M L V F L F . . N G F Y Q T S P L P P V D A A V S G Q Q M L T Q V V D L M K L I A<br />
VIII<br />
IX<br />
X<br />
XI<br />
F X<br />
P840<br />
P780<br />
P700
Figure 4S<br />
Cab. thermophilum PscB<br />
Chl. tepidum PscB<br />
Chl. limicola 245 PscB<br />
Chl. phaeobacteroides PscB<br />
Ptc. aestuarii PscB<br />
Cab. thermophilum PscB<br />
Chl. tepidum PscB<br />
Chl. limicola 245 PscB<br />
Chl. phaeobacteroides PscB<br />
Ptc. aestuarii PscB<br />
Cab. thermophilum PscB<br />
Chl. tepidum PscB<br />
Chl. limicola 245 PscB<br />
Chl. phaeobacteroides PscB<br />
Ptc. aestuarii PscB<br />
Cab. thermophilum PscB<br />
Chl. tepidum PscB<br />
Chl. limicola 245 PscB<br />
Chl. phaeobacteroides PscB<br />
Ptc. aestuarii PscB<br />
Cab. thermophilum PscB<br />
Chl. tepidum PscB<br />
Chl. limicola 245 PscB<br />
Chl. phaeobacteroides PscB<br />
Ptc. aestuarii PscB<br />
PscB Alignments<br />
10 20 30 40 50 60<br />
M A A E E K A E M A K D A S A E A P A E K A A T K A A A K T A A K S - - - - - - - - - -<br />
M A E P V E N K N Q A P A P G A K V P P K G A P A A P K A G A P A A P K G P V A P K A G A P A A K T G A S A A K Q A G K<br />
M A E P V K Q P D I S P K P A S K A A P A A P K A S V A P K A A T P A A K Q D Q A K P K A S S P K P - - - - - - S S G K<br />
M A E P V K Q P D V P P A P V S K A A P A A P K A A P K A A S P A A K Q G E - V K P K I S A A P K Q - - - - - - T S G K<br />
M A D P V K K Q E - - - - - T A S G P A K A K P A A P K A K A D A P K G K P A P K A G G A S S L V K G - - - - - - - - -<br />
M A E P V K . . P A P . K A A P A A A P K A A A P K . K . . A . A . K G A S A A K S G K<br />
70 80 90 100 110 120<br />
- - - - - - - - - - - - - - - - - A A A K A P A K A A A K A A P P P I P G - - - - - - - - - - - - P P S R - - - - - - -<br />
P R L A S L G V T L G R S G V R Q E S A L P Y V K P K A V P P P K P A A P - A A K G A P A P K G A P A A P - - - - - - -<br />
L R L E A L N V N L G R T G I R Q E S A L P V K K A A P K A A P K P A P G - A K P A A P A A K A A P A V G - - - - - - -<br />
I R L E A L N V N L G R T G I R Q E S A L P V K K A P P K A A P K P A P G - G A K P A P G A K P A P K T A G A P A G T P<br />
- R L D S L D V N L G R T G R R M E S A L P V V R P K P K A A P K L A P K G A P K G A P A G K A V P K G A P A A K G - -<br />
R L . L V N L G R T G . R Q E S A L P V . K A P K A A P K P A P G G A K . A P A . K . A P . . A G T P<br />
130 140 150 160 170 180<br />
- - - - - L S R P K T A A P K K K R E R Q I Y T I I E E L C I G C G F C T D E C P P K V N A I L P R D V E A V L D G G E<br />
- - A A K A A P G A P V A K A A P K A K K H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F<br />
- - A A P A P K G A P V A K A A P R A K K H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F<br />
V A K S A P A A G T P L A K A A P R A K S H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F<br />
- A P A A K P A A K P I A K K A P R P K K H Y F I L E N L C V G C G L C L D T C P P K V N A I G Y K F Y G D V Q E G G F<br />
V A A . G P . A K A A P R A K K H Y F I I E N L C V G C G L C L D K C P P K V N A I G Y K F Y G D V Q E G G F<br />
190 200 210 220 230 240<br />
T Y W I D Q T R C I S C S L C F V A G T C P T D A V V F T E G G V S R T Q Y M E D Y M H I E L V D E P Y W R T R D E L S<br />
R C Y I D Q A A C I S C S A C F S G D E C P S G A L I E V L P D G E V L D F S Y T P P E R L D F D L R F L H R F H R E A<br />
R C Y V D Q D A C I S C S A C F S G D E C P S G A L I E V L P D G Q I L D F S Y T P P E R L D F D L R F L H R F H R E A<br />
R C Y I D Q D A C I S C S A C F S T D E C P S G A L I E V L P D G Q V L D F S F T P P E R L D F D L R F L H R F H R E V<br />
R C Y I D Q D A C I S C A A C F S S D E C P S G A L I E I L P T G E V L D F T Y T P P E R L D F D L R F L H R F H K E S<br />
R C Y I D Q D A C I S C S A C F S . D E C P S G A L I E V L P D G V L D F S Y T P P E R L D F D L R F L H R F H R E .<br />
R L G S I L *<br />
R<br />
R<br />
R<br />
R<br />
R L G S I L<br />
250 260 270 280 290 300
Figure 5S<br />
FmoA Alignments<br />
10 20 30 40 50 60<br />
Cab. thermophilum FmoA<br />
M G S K L T T A Y Y D L D L D A T G - - - - W G T L R A E A R I S N V P S A S P L L P I D G E L K L E A K - - K<br />
Chl. tepidum FmoA M A L F G S N D V T T A H S D Y E I V L E G G S S S W G K V K A R A K V N - A P P A S P L L P A D C D V K L N V K P L D<br />
Chl. limicola FmoA<br />
M A L F G T K D T T T A H S D Y E I I L E G G A S S W G K V K G R A K V N - V P P A S P L L P A D C N V K I N V K P L D<br />
Chl. phaeobacteroides FmoA M A L F G T K D T T T A H S D Y E I I L E G G S S S W G K V K G R A K V N - V P P A S P L L P A D C N V K I N V K P L D<br />
Ptc. aestuarii FmoA M A L F G T K D T T T A H S D Y E I I L E G G S S S W G Q V K G R A K V N - V P A A L P L L P A D C N I K I E A K P L D<br />
M A L F G T K D T T T A H S D Y E I I L E G G S S S W G K V K G R A K V N N V P P A S P L L P A D C N V K I N V K P L D<br />
Cab. thermophilum FmoA<br />
Chl. tepidum FmoA<br />
Chl. limicola FmoA<br />
Chl. phaeobacteroides FmoA<br />
Ptc. aestuarii FmoA<br />
Cab. thermophilum FmoA<br />
Chl. tepidum FmoA<br />
Chl. limicola FmoA<br />
Chl. phaeobacteroides FmoA<br />
Ptc. aestuarii FmoA<br />
Cab. thermophilum FmoA<br />
Chl. tepidum FmoA<br />
Chl. limicola FmoA<br />
Chl. phaeobacteroides FmoA<br />
Ptc. aestuarii FmoA<br />
Cab. thermophilum FmoA<br />
Chl. tepidum FmoA<br />
Chl. limicola FmoA<br />
Chl. phaeobacteroides FmoA<br />
Ptc. aestuarii FmoA<br />
Cab. thermophilum FmoA<br />
Chl. tepidum FmoA<br />
Chl. limicola FmoA<br />
Chl. phaeobacteroides FmoA<br />
Ptc. aestuarii FmoA<br />
Cab. thermophilum FmoA<br />
Chl. tepidum FmoA<br />
Chl. limicola FmoA<br />
Chl. phaeobacteroides FmoA<br />
Ptc. aestuarii FmoA<br />
70 80 90 100 110 120<br />
I E D D V V R L T Y F G Q S I V D G I L G R V E G E T D I A N E S P T R R V A A G E G K I T V G K F S H R F E F E G V V<br />
P A K G F V R I S A V F E S I V D S T K N K L T I E A D I A N E T K E R R I S V G E G M V S V G D F S H T F S F E G S V<br />
P A K G F V R F S A V I E S I V D S T K N K L V V E A D I A N E T K E R R I C V G E G S V S V G D F S H S F S F E G S V<br />
P A K G F V R F S A V I E S I V D S T K N K L V V E A D I A N E T K E R R I C V G E G S V T V G D F S H S F S F E G S V<br />
T Q K G V V R F T S T I E S I V D S T K N K L V V E V D I A N E T K D R R V A V G E G E V T V G D F S H K F S F E G S V<br />
P A K G F V R F S A V I E S I V D S T K N K L V V E A D I A N E T K E R R I V G E G V T V G D F S H . F S F E G S V<br />
130 140 150 160 170 180<br />
D C L R Y W R S K A I D D N I V A R K Q R L L C G N L F H D L S V R V P L D S E E V I D T W L E M Q D A F R N - S P N F<br />
V N L F Y Y R S D A V R R N V P N - - P I Y M Q G R Q F H D I L M K V P L D N N D L I D T W E G T V K A I G S - T G A F<br />
V N M Y Y Y R S D A V R R N V P N - - P I Y M Q G R Q F H D I I M K V P L D N P D V I D T W E G T V K A V Q S - T G A F<br />
V N L F Y Y R S D A V R R N V P N - - P I Y M Q G R Q F H D I I M K V P L D N N D V I D T W E G T V K A L Q S - T G S F<br />
V N M Y Y F R S D A V R R N V P N - - P I Y M Q G R Q F H D I M M K V P L D N N D L I D T W E G F Q Q S I S G G G A N F<br />
V N L . Y Y R S D A V R R N V P N R K P I Y M Q G R Q F H D I . M K V P L D N N D V I D T W E G T V K A . S G T G F<br />
190 200 210 220 230 240<br />
G D Y I K D V W L I G P L W N A L E Q T G Q S L D N I D V Y Y F S E V E G - - G E K S R I D F R F A G G G T G I V D S I<br />
N D W I R D F W F I G P A F T A L N E G G Q R I S R I E V N G L N T E S G P K G P V G V S R W R F S H G G S G M V D S I<br />
N D W I R D F W F I G P A F T A L N E G G Q R I S R I E V N S I G T Q G S D K G P V G V S R W R F S H G G S G V V D S I<br />
N D W I R E F W F I G P A F T A L N E G G Q R I S R I E V N S I G T Q S G E K G P V G V S R W R F S H G G S G I V D S I<br />
G D W I R E F W F I G P A F T A I N E G G Q R I S P I Q V N N F G V E S G E K G P V G V S R W K F S H A G S G I V D S I<br />
N D W I R D F W F I G P A F T A L N E G G Q R I S R I E V N . G T S G . K G P V G V S R W R F S H G G S G I V D S I<br />
250 260 270 280 290 300<br />
S R W L E L F P I D G L G K P V N Q G G R V A R L Q G N F N A S V Q G I E V K L F V E L P G F S V P I E G G K R K V L N<br />
S R W A E L F P S D K L N R P A Q - - - - - - - V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L N<br />
S R W M E L F P S D K L N R P A S - - - - - - - V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L N<br />
S R W A E L F P S D K L N K P A S - - - - - - - V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L N<br />
S R W A E L F P V D Q L N K P A S - - - - - - - I E G G F R S D S Q G I E V K V D G N L P G V S R D A G G G L R R I L N<br />
S R W A E L F P S D K L N K P A S Q G G R V A R V E A G F R S D S Q G I E V K V D G E F P G V S V D A G G G L R R I L N<br />
310 320 330 340 350 360<br />
H P L V P L A H H G I A V N S E P A D L S I R F K V S I P K G K K F I E T A N S F E W D R V A E N V R V F T G G R Y K A<br />
H P L I P L V H H G M V G K F N N F N V D A Q L K V V L P K G Y K I R Y A A P Q Y R S Q N L E E Y R - - W S G G A Y A R<br />
H P L I P L V H H G M V G K F N D F T V D T Q L K I V L P K G Y K V R Y A A P Q F R S Q N L E E Y R - - W S G G A Y A R<br />
H P L I P L V H H G M V G K F N D F T V D T Q L K I V L P K G Y K V R Y A A P Q F R S Q N L E E Y R - - W S G G A Y A R<br />
H P L I P L V H H G M V G K F N D F T V D T Q L K V V L P K G Y K I R Y A A P Q F R S Q N L E E Y R - - W S G G A Y A R<br />
H P L I P L V H H G M V G K F N D F T V D T Q L K V V L P K G Y K . R Y A A P Q F R S Q N L E E Y R R V W S G G A Y A R<br />
370 380 390 400 410 420<br />
W A E G I C K G S Y S P F D I F F G<br />
W V E H V C K G G V G Q F E I L Y A Q<br />
W V E H V C K G G T G Q F E V L Y A Q<br />
W V E H V C K G G T G Q F E V L Y A Q<br />
W V E H V C K G G T G Q F E V L Y A Q<br />
W V E H V C K G G T G Q F E V L Y A Q<br />
Ligands to BChl a
Figure 6S<br />
A.<br />
B. bchG-orf-csmA bchY<br />
350,000<br />
0 50000 100000 150000 200000 250000 300000 350000 400000<br />
BAC M60-018 J19<br />
16S rRNA recA pscAB-fmoA<br />
BAC M60-018 J19<br />
450,000 550,000 650,000 750,000 850,000 950,000
Figure 7S<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
GFP1 16S rRNA<br />
Cab. thermophilum 16S rRNA<br />
16S rRNA Alignment<br />
10 20 30 40 50 60 70 80 90 100 110 120<br />
T C G A A C G G G A A A G T C C T T C G G G A C G A G T A C A G T G G C G C A C G G G T G A G T A A C G C G T G G G T T A T C T A<br />
G A G A G T T T G A T C C T G G C T C A G A A T C A A C G C T G G C G G C A T G C C T A A C A C A T G C A A G T C G A A C G G G A A A G T C C T T C G G G A C G A G T A C A G T G G C G C A C G G G T G A G T A A C G C G T G G G T T A T C T A<br />
G A G A G T T T G A T C C T G G C T C A G A A T C A A C G C T G G C G G C A T G C C T A A C A C A T G C A A G T C G A A C G G G A A A G T C C T T C G G G A C G A G T A C A G T G G C G C A C G G G T G A G T A A C G C G T G G G T T A T C T A<br />
130 140 150 160 170 180 190 200 210 220 230 240<br />
C C T C C G G G T G G G G A A T A A C T C G G G G A A A C T C G A G C T A A T A C C G C A T A A T A C G T - C T A C G T T A A A G C A G C A A T G C G C C T G G A G A T G A G C C T G C G T C C G A T T A G C T A G T T G G T G G G G T A A T G<br />
C C T C C G G G T G G G G A A T A A C T C G G G G A A A C T C G A G C T A A T A C C G C A T A A T A C G T T C T A C G T T A A A G C A G C A A T G C G C C T G G A G A T G A G C C T G C G T C C G A T T A G C T A G T T G G T G G G G T A A T G<br />
C C T C C G G G T G G G G A A T A A C T C G G G G A A A C T C G A G C T A A T A C C G C A T A A T A C G T T C T A C G T T A A A G C A G C A A T G C G C C T G G A G A T G A G C C T G C G T C C G A T T A G C T A G T T G G T G G G G T A A T G<br />
250 260 270 280 290 300 310 320 330 340 350 360<br />
G C C C A C C A A G G C G A C G A T C G G T A G C C G G C C T G A G A G G G C G G T C G G C C A C A C T G G C A C T G A A A C A C G G G C C A G A C T C C T A C G G G A G G C A G C A G T G A G G A A T C T T G G G C A A T G G G C G A A A G C<br />
G C C C A C C A A G G C G A C G A T C G G T A G C C G G C C T G A G A G G G C G G T C G G C C A C A C T G G C A C T G A A A C A C G G G C C A G A C T C C T A C G G G A G G C A G C A G T G A G G A A T C T T G G G C A A T G G G C G A A A G C<br />
G C C C A C C A A G G C G A C G A T C G G T A G C C G G C C T G A G A G G G C G G T C G G C C A C A C T G G C A C T G A A A C A C G G G C C A G A C T C C T A C G G G A G G C A G C A G T G A G G A A T C T T G G G C A A T G G G C G A A A G C<br />
370 380 390 400 410 420 430 440 450 460 470 480<br />
C T G A C C C A G C A A T G C C G C G T G A A C G A T G A A G T C C T T C G G G A T G T A A A G T T C A T A A G C A G G A G A A G A A C A C C A T G A C G G T A T C C T G T G T A A G C C C C G G C C A A C T A C G T G C C A G C A G C C G C G<br />
C T G A C C C A G C A A T G C C G C G T G A A C G A T G A A G T C C T T C G G G A T G T A A A G T T C A T A A G C A G G A G A A G A A C A C C A T G A C G G T A T C C T G T G T A A G C C C C G G C C A A C T A C G T G C C A G C A G C C G C G<br />
C T G A C C C A G C A A T G C C G C G T G A A C G A T G A A G T C C T T C G G G A T G T A A A G T T C A T A A G C A G G A G A A G A A C A C C A T G A C G G T A T C C T G T G T A A G C C C C G G C C A A C T A C G T G C C A G C A G C C G C G<br />
490 500 510 520 530 540 550 560 570 580 590 600<br />
G T A A T A C G T A G G G G G C A A G C G T T G T T C G G A T T T A C T G G G C G T A A A G G G C G C G T A G G C G G C G C G T A A A G T C G G A C A T G A A A T C A T T A G G C T C A A C C T A A T G G C T G T G T C C G A T A C T T A C G C<br />
G T A A T A C G T A G G G G G C A A G C G T T G T T C G G A T T T A C T G G G C G T A A A G G G C G C G T A G G C G G C G C G T A A A G T C G G A C A T G A A A T C A T T A G G C T C A A C C T G A T G G C T G T G T C C G A T A C T T A C G T<br />
G T A A T A C G T A G G G G G C A A G C G T T G T T C G G A T T T A C T G G G C G T A A A G G G C G C G T A G G C G G C G C G T A A A G T C G G A C A T G A A A T C A T T A G G C T C A A C C T R A T G G C T G T G T C C G A T A C T T A C G Y<br />
610 620 630 640 650 660 670 680 690 700 710 720<br />
G C T T G G A G T G C G G G A G A G G C A G C C G G A A T T C T C G G T G T A G C G G T G A A A T G C G T A G A T A T C G A G A G G A A C A C C G G T G A C G A A G G T G G G T T G C T G G A C C G A C A C T G A C G C T G A G G C G C G A A A<br />
G C T T G G A G T G C G G G A G A G G C A G C C G G A A T T C T C G G T G T A G C G G T G A A A T G C G T A G A T A T C G A G A G G A A C A C C G G T G A C G A A G G T G G G T T G C T G G A C C G A C A C T G A C G C T G A G G C G C G A A A<br />
G C T T G G A G T G C G G G A G A G G C A G C C G G A A T T C T C G G T G T A G C G G T G A A A T G C G T A G A T A T C G A G A G G A A C A C C G G T G A C G A A G G T G G G T T G C T G G A C C G A C A C T G A C G C T G A G G C G C G A A A<br />
730 740 750 760 770 780 790 800 810 820 830 840<br />
G C T A G G G G A G C A A A C G G G A T T A G A T A C C C C G G T A G T C C T A G C C C T A A A C G A T G A A T A C T T G A T G T G C G A G T T A C T A G G C A C T C G C G T G T C G A A G C T A A C G C G A T A A G T A T T C C G C C T G G G<br />
G C T A G G G G A G C A A A C G G G A T T A G A T A C C C C G G T A G T C C T A G C C C T A A A C G A T G A A T A C T T G A T G T G C G A G T T A C T A G G C A C T C G C G T G T C G A A G C T A A C G C G A T A A G T A T T C C G C C T G G G<br />
G C T A G G G G A G C A A A C G G G A T T A G A T A C C C C G G T A G T C C T A G C C C T A A A C G A T G A A T A C T T G A T G T G C G A G T T A C T A G G C A C T C G C G T G T C G A A G C T A A C G C G A T A A G T A T T C C G C C T G G G<br />
850 860 870 880 890 900 910 920 930 940 950 960<br />
G A G T A T G A C C G C A A G G T T G A A A C T C A A A G G A A T N G A C G G G G A C C C G C A C A A G C G G T G G A G C A T G T G G T T T A A T T C G A C G C A A C G C G A A G A A C C T T A C C T G A G T T C A A G A T A C G A T G A A G C<br />
G A G T A T G A C C G C A A G G T T G A A A C T C A A A G G A A T T G A C G G G G A C C C G C A C A A G C G G T G G A G C A T G T G G T T T A A T T C G A C G C A A C G C G A A G A A C C T T A C C T G A G T T C A A G A T A C G A T G A A G C<br />
G A G T A T G A C C G C A A G G T T G A A A C T C A A A G G A A T T G A C G G G G A C C C G C A C A A G C G G T G G A G C A T G T G G T T T A A T T C G A C G C A A C G C G A A G A A C C T T A C C T G A G T T C A A G A T A C G A T G A A G C<br />
970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080<br />
G C C G G A A T C G G C G T G G C C G G G C A A C C G G A G T C G T A T C A G G T G C T G C A T G G C T G T C G T C A G C T C G T G T C G T G A G A T G T T G G G T T A A G T C C C G C A A C G A G C G C A A C C C C T G T C G C C A G T T G C<br />
G C C G G A A T C G G C G T G G C C G G G C A A C C G G A G T C G T A T C A G G T G C T G C A T G G C T G T C G T C A G C T C G T G T C G T G A G A T G T T G G G T T A A G T C C C G C A A C G A G C G C A A C C C C T G T C G C C A G T T G C<br />
G C C G G A A T C G G C G T G G C C G G G C A A C C G G A G T C G T A T C A G G T G C T G C A T G G C T G T C G T C A G C T C G T G T C G T G A G A T G T T G G G T T A A G T C C C G C A A C G A G C G C A A C C C C T G T C G C C A G T T G C<br />
1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200<br />
C A C C A G T T C G G C T G A G C A C T C T G G C G A G A C C G C T G A T G A T G A A T C A G A G G A A G G T G G G G A T G A C G T C A A G T C C T C A T G G C C C T T A T G C T C A G G G C T A C A C A C G T G C T A C A A T G G T G A A T A<br />
C A C C A G T T C G G C T G A G C A C T C T G G C G A G A C C G C T G A T G A T G A A T C A G A G G A A G G T G G G G A T G A C G T C A A G T C C T C A T G G C C C T T A T G C T C A G G G C T A C A C A C G T G C T A C A A T G G T G A A T A<br />
C A C C A G T T C G G C T G A G C A C T C T G G C G A G A C C G C T G A T G A T G A A T C A G A G G A A G G T G G G G A T G A C G T C A A G T C C T C A T G G C C C T T A T G C T C A G G G C T A C A C A C G T G C T A C A A T G G T G A A T A<br />
1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320<br />
A C A A A G A G A T G C A A A C C C G C G A G G G G G A G C G A A T C T C A A A A A T T T C A C C T C A G T T C G G A T T G C A G T C T G C A A C T C G A C T G C A T G A A G T C G G A A T C G C T A G T A A T C G C A G A T C A G C A C G C T<br />
A C A A A G A G A T G C A A A C C C G C G A G G G G G A G C G A A T C T C A A A A A T T T C A C C T C A G T T C G G A T T G C A G T C T G C A A C T C G A C T G C A T G A A G T C G G A A T C G C T A G T A A T C G C A G A T C A G C A C G C T<br />
A C A A A G A G A T G C A A A C C C G C G A G G G G G A G C G A A T C T C A A A A A T T T C A C C T C A G T T C G G A T T G C A G T C T G C A A C T C G A C T G C A T G A A G T C G G A A T C G C T A G T A A T C G C A G A T C A G C A C G C T<br />
1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440<br />
G C G G T G A A T A C G T T C C C G G G T C T T G T A C A C A C C G C C C G T C A C A T C A C G A A A G T A G G C T G C A C T A G A A G T G G C T G A G C T A A C C C G<br />
G C G G T G A A T A C G T T C C C G G G T C T T G T A C A C A C C G C C C G T C A C A T C A C G A A A G T A G G C T G C A C T A G A A G T G G C T G A G C T A A C C C G C A A G G G A G G C A G G T T A C A A C G G T G T G G T C T A T G A T T<br />
G C G G T G A A T A C G T T C C C G G G T C T T G T A C A C A C C G C C C G T C A C A T C A C G A A A G T A G G C T G C A C T A G A A G T G G C T G A G C T A A C C C G C A A G G G A G G C A G G T T A C A A C G G T G T G G T C T A T G A T T<br />
1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560<br />
G G G G T G A A G T C G T A A C A A G G T A G C T G T A G G A G A A C C T G T G G C T G G A T C A C C T C C T T<br />
G G G G T G A A G T C G T A A C A A G G T A G C T G T A G G A G A A C C T G T G G C T G G A T C A C C T C C T T
Figure 8S<br />
99<br />
94<br />
75<br />
77<br />
95<br />
83<br />
81<br />
70<br />
100<br />
99<br />
73<br />
99<br />
96<br />
92<br />
99<br />
99<br />
79<br />
96 99<br />
99<br />
97<br />
99<br />
92<br />
99<br />
84<br />
91<br />
71<br />
99<br />
70<br />
77<br />
99<br />
99<br />
80<br />
95<br />
99<br />
88<br />
99<br />
70<br />
73<br />
100<br />
96<br />
89<br />
99<br />
99<br />
99<br />
99<br />
100<br />
99<br />
Deinococcus radiodurans DR1105<br />
Dehalococcoides ethenogenes<br />
Herpetosiphon aurantiacus<br />
Chloroflexus aurantiacus J-10-fl<br />
Roseiflexus sp. RS1<br />
Roseiflexus castenholzii<br />
Thermoanaerobacter tengcongensis<br />
Thermoanaerobacter ethanolicus<br />
Clostridium novyi<br />
Clostridium botulinum<br />
Clostridium acetobutylicum<br />
Pelotomaculum thermopropionicum<br />
Carboxydothermus hydrogeno<strong>for</strong>mans<br />
Heliobacterium modesticaldum<br />
Desulfitobacterium hafniense<br />
Streptococcus pyogenes<br />
Staphylococcus aureus<br />
Listeria monocytogenes<br />
Bacillus subtilis<br />
Bacillus cereus<br />
Bacillus anthracis<br />
Aquifex pyrophilus<br />
Aquifex aeolicus<br />
Deinococcus radiodurans<br />
Thermus thermophilus<br />
Thermus aquaticus<br />
Corynebacterium glutamicum<br />
Brevibacterium linens<br />
Arthrobacter aurescens<br />
Streptomyces coelicolor<br />
Nocardia farcinica<br />
Erythrobacter litoralis<br />
Blastochloris viridis<br />
Rhodopseudomonas palustris<br />
Bradyrhizobium japonicum<br />
Roseobacter denitrificans<br />
Rhodobacter sphaeroides<br />
Rhodobacter capsulatus<br />
Shewanella oneidensis<br />
Proteus vulgaris<br />
Shigella flexneri<br />
Escherichia coli<br />
Halorhodospira halophila<br />
Dechloromonas aromatica<br />
Bordetella pertussis<br />
Bordetella parapertussis<br />
Stigmatella aurantiaca<br />
Anaeromyxobacter sp.<br />
Anaeromyxobacter dehalogens<br />
Geobacter lovleyi<br />
Geobacter sulfurreducens<br />
Geobacter metallireducens<br />
Chloracidobacterium thermophilum<br />
Solibacter usitatus Ellin6076<br />
Acidobacteria sp. Ellin345<br />
Campylobacter jejuni<br />
Thiomicrospira denitrificans<br />
Wolinella succinogenes<br />
Helicobacter pylori<br />
Chloroherpeton thalassium<br />
Chlorobium ferooxidans<br />
Pelodictyon luteolum DSM 273<br />
Chlorobium phaeovibriodes DSM 265<br />
Chlorobium chlorochromatii<br />
Pelodictyon phaeoclathrati<strong>for</strong>me<br />
Prosthecochloris aesturarii DSM 271<br />
Chlorobium phaeobacteroides DSM 266<br />
Chlorobium limicola DSM 245<br />
Chlorobium phaeobacteroides BS1<br />
Chlorobium tepidum<br />
Chlorobaculum parvum DSM 263<br />
Cytophaga hutchinsonii<br />
Porphyromonas gingivalis<br />
Bacteriodes thetaiotaomicron<br />
Bacteriodes fragilis<br />
Petrotoga mobilis<br />
Thermotoga maritima<br />
Thermosipho melanesiensis<br />
Fervidobacterium nodosum<br />
Gloeobacter violaceus PCC 7421<br />
Synechococcus sp. 2-3B’a(2-13)<br />
Synechococcus sp. JA-3-3Ab<br />
Synechocystis sp. PCC 6803<br />
Synechococcus sp. PCC 7002<br />
Synechococcus sp. PCC 6301<br />
Nostoc sp. PCC 7120<br />
Nostoc puncti<strong>for</strong>me PCC 73102<br />
Chloroflexi<br />
Firmicutes (“Clostridia”)<br />
Firmicutes (“Bacilli”)<br />
Aquificae<br />
Deinococcus-Thermus<br />
Actinobacteria<br />
α-Proteobacteria<br />
γ-Proteobacteria<br />
β-Proteobacteria<br />
δ-Proteobacteria<br />
Acidobacteria<br />
ε-Proteobacteria<br />
Chlorobi<br />
Bacteriodetes<br />
Thermotogae<br />
Cyanobacteria
Cab. thermophilum RecA<br />
Solibacter usitatus RecA<br />
Acidobacteria sp. Ellin345 RecA<br />
Dehalococcoides ethenogenes RecA<br />
Herpetosiphon aurantiacus RecA<br />
Chloroflexus aggregans RecA<br />
Cfx. aurantiacus J-10-fl RecA<br />
Roseiflexus sp. RS1 RecA<br />
Roseiflexus castenholzii RecA<br />
The. tengcongensis RecA<br />
The. ethanolicus RecA<br />
Clostridium novyi RecA<br />
Clostridium botulinum RecA<br />
Clostridium acetobutylicum RecA<br />
Pel. thermopropionicum RecA<br />
Car. hydrogeno<strong>for</strong>mans RecA<br />
Hb. modesticaldum RecA<br />
Desulfitobacterium hafniense RecA<br />
Streptococcus pyogenes RecA<br />
Staphylococcus aureus RecA<br />
Listeria monocytogenes RecA<br />
Bacillus subtilis RecA<br />
Bacillus cereus RecA<br />
Bacillus anthracis RecA<br />
Aquifex pyrophilus RecA<br />
Aquifex aeolicus RecA<br />
Deinococcus radiodurans RecA<br />
Thermus thermophilus RecA<br />
Thermus aquaticus RecA<br />
Corynebacterium glutamicum RecA<br />
Brevibacterium linens RecA<br />
Arthrobacter aurescens RecA<br />
Streptomyces coelicolor RecA<br />
Nocardia farcinica RecA<br />
Erythrobacter litoralis RecA<br />
Blastochloris viridis RecA<br />
Rhodopseudomonas palustris RecA<br />
Bradyrhizobium japonicum RecA<br />
Roseobacter denitrificans RecA<br />
Rhodobacter sphaeroides RecA<br />
Rhodobacter capsulatus RecA<br />
Shewanella oneidensis RecA<br />
Proteus vulgaris RecA<br />
Shigella flexneri RecA<br />
Escherichia coli RecA<br />
Halorhodospira halophila RecA<br />
Dechloromonas aromatica RecA<br />
Bordetella pertussis RecA<br />
Bordetella parapertussis RecA<br />
Stigmatella aurantiaca RecA<br />
Anaeromyxobacter sp. RecA<br />
Anaeromyxobacter dehalogens RecA<br />
Geobacter lovleyi RecA<br />
Geobacter sulfurreducens RecA<br />
Geobacter metallireducens RecA<br />
Campylobacter jejuni RecA<br />
Thiomicrospira denitrificans RecA<br />
Wolinella succinogenes RecA<br />
Helicobacter pylori RecA<br />
Chloroherpeton thalassium RecA<br />
Chlorobium ferooxidans RecA<br />
Pelodictyon luteolum RecA<br />
Chlorobium vibrio<strong>for</strong>me RecA<br />
Chlorobium chlorochromatii RecA<br />
Pld. .phaeoclathrati<strong>for</strong>me RecA<br />
Prosthecochloris aesturarii RecA<br />
Chlorobium phaeobacteroides RecA<br />
Chlorobium limicola RecA<br />
Chlorobium phaeobacteroides BS1<br />
Chlorobium tepidum RecA<br />
Chlorobaculum parvum RecA<br />
Cytophaga hutchinsonii RecA<br />
Porphyromonas gingivalis RecA<br />
Bacteriodes thetaiotaomicron RecA<br />
Bacteriodes fragilis RecA<br />
Petrotoga mobilis RecA<br />
Thermotoga maritima RecA<br />
Thermosipho melanesiensis RecA<br />
Fervidobacterium nodosum RecA<br />
Gloeobacter violaceus RecA<br />
Synechococcus sp. JA-2-3B'a(2-13)<br />
Synechococcus sp. JA-3-3Ab RecA<br />
Synechocystis sp. PCC 6803 RecA<br />
Synechococcus sp. PCC 7002 RecA<br />
Synechococcus sp. PCC 6301 RecA<br />
Nostoc sp. PCC 7120 RecA<br />
Nostoc puncti<strong>for</strong>me PCC 73102 RecA<br />
Deinococcus radiodurans DR1105<br />
1 60<br />
1 59<br />
1 61<br />
1 58<br />
1 58<br />
1 0<br />
1 60<br />
1 60<br />
1 60<br />
1 57<br />
1 56<br />
1 57<br />
1 61<br />
1 57<br />
1 57<br />
1 57<br />
1 56<br />
1 59<br />
1 71<br />
1 57<br />
1 56<br />
1 56<br />
1 56<br />
1 56<br />
1 63<br />
1 60<br />
1 70<br />
1 57<br />
1 57<br />
1 70<br />
1 68<br />
1 59<br />
1 58<br />
1 60<br />
1 68<br />
1 56<br />
1 70<br />
1 69<br />
1 70<br />
1 70<br />
1 70<br />
1 59<br />
1 59<br />
1 59<br />
1 59<br />
1 59<br />
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1 66<br />
1 66<br />
1 61<br />
1 61<br />
1 61<br />
1 57<br />
1 58<br />
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1 57<br />
1 57<br />
1 59<br />
1 59<br />
1 71<br />
1 71<br />
1 70<br />
1 71<br />
1 72<br />
1 70<br />
1 70<br />
1 70<br />
1 70<br />
1 70<br />
1 71<br />
1 72<br />
1 66<br />
1 66<br />
1 72<br />
1 74<br />
1 64<br />
1 60<br />
1 60<br />
1 59<br />
1 74<br />
1 56<br />
1 56<br />
1 60<br />
1 61<br />
1 63<br />
1 61<br />
1 61<br />
1 149<br />
M T D T R Q E R N K A I D A A I A S L E K Q F G K G V V M R L G E R - - - - - - - - - - - Q A L E V P A I S T T C L S L D A A I G I G G V P R<br />
M D E K E R S R A L E T A L G Q I E K Q F G K G S I L R L G S K D - - - - - - - - - - A I V P V S V I S T G S I S L D A A L G V G G F P R<br />
M S D E R T E R G R S L E L A L S Q I E K Q F G K G S I M K L G A K G - - - - - - - - - - A I V P I S V I S T G A I S F D A A L G T G G F P R<br />
M T T E K D K A L E L T V G I I E K R F G K G S I M K L S D P T F - - - - - - - - - - R Q T V E F I P T S S L A L D I A L G V G G I P R<br />
M A I S P E K E K A L A A A L S Q I D R Q F G K G A I M R M S D Q - - - - - - - - - - - - R L S I E A I P T G S I A L D L A L G V G G I P R<br />
M A I T P E K E K A L A A T M A Q I D R K F G K G S I M K M G E A S S - - - - - - - - - - R V A I E A I P T G S I A L D I A L G I G G V P R<br />
M A L S P E K E K A L A A A M S Q I D R K Y G K G S I M R M G E V S S - - - - - - - - - - K L A I E V I P T G S I A L D I A L G V G G V P R<br />
M A L S P E K E K A L A A A M S Q I D R K Y G K G S I M R M G E A S S - - - - - - - - - - K L A I E V I P T G S I A L D I A L G V G G V P R<br />
M M I E K Q K A L E M A I S Q I E R Q F G K G - A I M R L G D T A - - - - - - - - - - K L N V E V I P T G S L E L D I A L G V G G V P R<br />
M I E K Q K A L E M A I S Q I E R Q F G K G - S I M R L G D T A - - - - - - - - - - K L N V E V I P T G S L E L D I A L G V G G V P R<br />
M N N E K M K A L Q A A I T Q I E K Q F G K G - S V M K L G E E H - - - - - - - - - - V L N V E A I S T G S L S L D I A L G I G G I P R<br />
M A N K V N P E K L K A I E A A M G Q I E K Q F G K G - S I M K L G E D S - - - - - - - - - - I L N V E S I S T G S L D L D I A L G I G G V P R<br />
M D N E K L K A I D A A M S Q I E K Q F G K G - A I M K L G E Q D - - - - - - - - - - I L N I D A I S T G C L S L D I A L G I G G V P R<br />
M S D R Q K A L E L A L M Q I E R Q F G K G S I M K L G E A S A - - - - - - - - - - K L N V E V I P T G A L A L D I A L G V G G V P R<br />
M N D K L K A L E L T I S Q I E K Q F G K G S I M R L G E N T G - - - - - - - - - - K F Q V E V I P T G S L A L D L A L G V G G V P R<br />
D N K L Q A L Q Q A L N S I E K A F G K G S I M R L G E A S A - - - - - - - - - - K L L V E V I P T G S I A L D M A L G I G G V P R<br />
M A G N D K L K A L D M A L S Q I E K Q F G K G S I M R L G E A S A - - - - - - - - - - K M A V E V I P T G C L A I D I A L G V G G V P R<br />
M A K K L K K N E E I T K K F G D E R R K A L D D A L K N I E K D F G K G A V M R L G E R A E - - - - - - - - - - - Q K V Q V M S S G S L A L D I A L G A G G Y P K<br />
M D N D R Q K A L D T V I K N M E K S F G K G A V M K L G D N I G - - - - - - - - - - - R R V S T T S T G S V T L D N A L G V G G Y P K<br />
M N D R Q A A L D Q A L K Q I E K Q F G K G S I M K L G E H S D - - - - - - - - - - - Q N I S T I S S G S L A L D I A L G V G G Y P R<br />
M S D R Q A A L D M A L K Q I E K Q F G K G S I M K L G E K T D - - - - - - - - - - - T R I S T V P S G S L A L D T A L G I G G Y P R<br />
M S D R Q A A L D M A L K Q I E K Q F G K G S I M K L G E Q A E - - - - - - - - - - - R K V S T V S S G S L A L D V A L G V G G Y P R<br />
M S D R Q A A L D M A L K Q I E K Q F G K G S I M K L G E Q A E - - - - - - - - - - - R K V S T V S S G S L A L D V A L G V G G Y P R<br />
M A R V S E N L S E K M K A L E V A L S S I E K R F G K G A V M P L K A V E T - - - - - - - - - - - V E V E T I P T G S I S L D I A T G V G G I P K<br />
M S E N L S E K K K A L E V A L S N I E K R F G K G A V M P L K S V E K - - - - - - - - - - - V Q V E T I P T G S L A L D I A T G V G G I P K<br />
M S K D A T K E I S A P T D A K E R S K A I E T A M S Q I E K A F G K G S I M K L G A E S K - - - - - - - - - - - L D V Q V V S T G S L S L D L A L G V G G I P R<br />
M D E S K R K A L E N A L K A I E K E F G K G A V M R L G E M P K - - - - - - - - - - - Q Q V D V I P T G S L A L D L A L G I G G I P R<br />
M D E S K R K A L E N A L K A I E K E F G K G A V M R L G E M P K - - - - - - - - - - - Q Q V D V I P T G S L A L D L A L G I G G I P R<br />
M A P K K T A T K A T A A K G N D R Q K A L D A A L A L I E K D F G K G A V M R L G - D E N - - - - - - - - - - R P P I Q T I S S G N T A I D I A L G I G G F P R<br />
M A R T P K N L Q I P T G G D K S K A L D A A L G Q I D R N Y G K G A I M R L G - E G V - - - - - - - - - - R E P I A C I P T G S I A L D I A L G I G G L P R<br />
M A A T P D R E K A L E A A L A Q I D K Q F G K G S I M R L G - D D T - - - - - - - - - - R A P I E V I P T G S I A L D V A L G I G G L P R<br />
M A G T D R E K A L D A A L A Q I E R Q F G K G A V M R M G - D R T - - - - - - - - - - N E P I E V I P T G S T A L D V A L G V G G I P R<br />
M A P Q A Y D R D K A L E L A L A Q V E K S F G K G A V M R L G - E E A - - - - - - - - - - R Q P I S V I P T G S I A L D V A L G I G G L P R<br />
M S A N L K L V E K E N N V D R Q K A L D A A L A Q I D R A F G K G S A M K L G S K E - - - - - - - - - - - A M N V E A I S T G S L G L D I A L G I G G L P K<br />
M D K T K A L D A A L T Q I E R A F G K G S I M R L G K S G A - - - - - - - - - - A L E V E T I S T G S L G L D I A L G I G G L P R<br />
M A A P T A L R I V E G S S M D K S K A L S A A L S Q I E R Q F G K G S V M R L G K N D R - - - - - - - - - - S M D I E T I S S G S L G L D I A L G V G G L P K<br />
M S A T A L R I V E G S S M D K S K A L A A A L S Q I E R Q F G K G S V M K L G K N D R - - - - - - - - - - S M D V E A V S S G S L G L D I A L G I G G L P K<br />
M A T A D L L S M N S K K S A D K Q K A L D S A L A Q I E R Q F G K G S I M K L G A E G A - - - - - - - - - - V Q D I Q A S S T G S L G L D I A L G I G G L P M<br />
M A T A N L L D L N G R R E M D K A K A L E S A L A Q I E R Q F G K G S I M K L G A N S P - - - - - - - - - - V M E I E A T S T G S L G L D I A L G I G G L P K<br />
M A T T G L F E M N D K G K A D K Q K A L E C A L A Q I E R Q F G K G S I M K L G G D T P - - - - - - - - - - P P E I E A T S T G S L G L D I A L G I G G L P K<br />
M K V D P N K E K A L A A V L S Q I E K Q F G K G S I M K L G E D - - - - - - - - - - - R S M D V E T I S T G S L S L D V A L G A G G L P M<br />
M A I D E N K Q K A L A A A L G Q I E K Q F G K G S I M R L G E D - - - - - - - - - - - R S M D V E T I S T G S L S L D I A L G A G G L P M<br />
M A I D E N K Q K A L A A A L G Q I E K Q F G K G S I M R L G E D - - - - - - - - - - - R S M D V E T I S T G S L S L D I A L G A G G L P M<br />
M A I D E N K Q K A L A A A L G Q I E K Q F G K G S I M R L G E D - - - - - - - - - - - R S M D V E T I S T G S L S L D I A L G A G G L P M<br />
M D D D R K K A L G A A L G Q I E K Q F G K G A V M R M G D A R - A - - - - - - - - V R G D V K S I S T G S L A L D V A L G I G G L P R<br />
M D D N K A K A L A A A L Q Q I E K Q F G K G S I M K M G D A - - E - - - - - - - - I D E G I Q V V S T G S L G L D I A L G V G G L P R<br />
M D D K T S K A A A A E K A K A L A A A L S Q I E K Q F G K G S I M R Y G D N - - E - - - - - - - - V E H D I Q V V S T G S L G L D I A L G V G G L P R<br />
M D D K T S K A A A A E K A K A L A A A L S Q I E K Q F G K G S I M R Y G D N - - E - - - - - - - - V E H D I Q V V S T G S L G L D I A L G V G G L P R<br />
M A V N S E K E K A I E L A M S A V E R Q F G K G S I M R L G K D E P - - - - - - - - - L T R D I Q A I S T G S I S L D I A L G V G G V P K<br />
M A V A P E K E K A I E L A V S S I E K A F G K G S I M R L G N E E A - - - - - - - - - L V K D V Q A V S T G S V S L D I A L G V G G F P R<br />
M P V G P E K E K A I E L A V A S I E K A F G K G S I M R L G N E D A - - - - - - - - - L V K D V Q A V S T G A I S L D I A L G V G G F P R<br />
M S D K N K A I E L A L S Q I E K Q F G K G A I M R L G N D E - - - - - - - - - - V L P G V E S I S T G A I S L D L A L G V G G V P R<br />
M T Q E R E K A I E L A L S Q I E K Q F G K G A I M R L G A D E - - - - - - - - - - A L P D V A A I P T G S L S L D L A L G V G G V P R<br />
M T Q E R E K A I E L A L S Q I E K Q F G K G A I M R L G A D E - - - - - - - - - - A L P D I D A I P T G A L S L D I A L G V G G V P R<br />
M D D N K R K S L D A A L K S L D K T F G K G T I L R L G - D K E - - - - - - - - - - V E Q I D S I G T G S V G L D L A L G I G G V P K<br />
M D A N K Q K S L D L A M K Q I D K A F G K G A L M R L G - D K E - - - - - - - - - - I M P I E S I S T G S L G L D L A L G I G G I P Q<br />
M A L D E N K R K A I D L A I K Q I D K A F G K G A L V R L G - E K P - - - - - - - - - - V E K I D S I S T G S L G L D I A L G I G G I P Q<br />
M A I D E D K Q K A I S L A I K Q I D K V F G K G A L V R L G - D K Q - - - - - - - - - - V E K I D S I S T G S L G L D L A L G I G G V P K<br />
M A K E K A S S Q E Q E Q K V K D E K L K Q L N L A V E S L E K Q F G K G A I M R L G - D D A - - - - - - - - - - V V Q V P V V S T G S I S L D Y A L G V G G L P K<br />
M D T Q K I E Q S V P P V V D P A K L K Q L N L A V D A L E K Q F G K G A I M R L G D S S A - - - - - - - - - - V M Q V L S I S T G S M T L D F A L G V G G L P R<br />
M A T P R P A E T A P G V D P A K L K Q L N L A V E A L E K Q F G K G A I M R L G D D S A - - - - - - - - - - V M Q V Q S I S T G S M T L D F A L G V G G L P R<br />
M D T T S K K E K K V P G I D P A K L K Q L D L A V D S L E K Q F G K G A I M R L G D D S A - - - - - - - - - - V M Q V Q A I S T G S M T L D F A L G V G G L P R<br />
M T M D N P K V E Q A G H A V D S A K L K Q L N L A V D A L E K Q F G K G T I M R M G D G S A - - - - - - - - - - G L T V Q A I S T G S M A L D F A L G V G G L P R<br />
M D T Q K I E K K T V T V D P A K L K Q L N L A V E A L E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M K V Q S I S T G S M T L D F A L G V G G L P R<br />
M D T Q K I E K K T V T V D P A K L K Q L N L A V E A L E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M K V Q S I S T G S M T L D F A L G V G G L P R<br />
M D N I K T E Q K S D L V D P A K L K Q L N L A V D A I E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M H V Q A I S T G S M T L D F A L G V G G L P R<br />
M D N I K T E Q K S D L V D P A K L K Q L N L A V D A I E K Q F G K G A I M R L G D D S A - - - - - - - - - - G M H V Q A I S T G S M T L D F A L G V G G L P R<br />
M D T K K N V Q E K K T A D P A K L K Q L S L A V E T L E K Q F G K G A I M C L G D Q G A - - - - - - - - - - I I P V Q S V S T G S I A L D F A L G V G G F P R<br />
M E K E A T P Q Q Q P P V V D P A R L K Q L N L A I E T L E K Q F G K G A I M R L G D D S A - - - - - - - - - - V M H V Q V I S T G S M A L D Y A L G V G G L P R<br />
M D K E N S K Q T A Q P P V V D P A R L K Q L N L A I E T L E K Q F G K G S I M R L G D D S A - - - - - - - - - - V M H V K A I S T G S M A L D Y A L G V G G F P R<br />
M S K S T S T P N P Q N E K L K A L Q L T I D K L E K T Y G K G T V M K L S - D E V - - - - - - - - - - V M D V P V I S T G S L G L D I A L G I G G L P K<br />
M A E E K I P T V Q D E K K L Q A L R M A T E K I E K T F G K G A I M N M G - A N T - - - - - - - - - - V E D V S V I P S G S I G L D L A L G V G G Y P R<br />
M A K K D E L N F E T D N - K M A S S E K L K A L Q A T M E K I E K N F G K G S I M K M G - D D S - - - - - - - - - - V E Q V E V I P T G S I A L N V A L G V G G Y P R<br />
M A K K E D E L N F E T D N N K M A S S E K L K A L Q A A M D K I E K S F G K G S I M K M G - E E V - - - - - - - - - - V E Q V E V I P T G S I A L N A A L G V G G Y P R<br />
M A K N D S K D A N K E E L L E K L V K E L E K N H G T G S V M L M G K G L D - - - - - - - - - - N S N L A V V P S G C L S L D I A L G V G G Y P R<br />
M P E E K Q K K S V L E K A L K R I E E N F G K G S I M I L G D E T Q - - - - - - - - - - V Q P V E V I P T G S L A I D I A T G V G G Y P R<br />
M S D N K Q K K E V L K K A I G K I E K T F G K G S I M F L G E E N A - - - - - - - - - - V Q N I E T I S S G S V A I D I A S G V G G Y P R<br />
M S D E M K K D V L K K A I S K I E K T Y G K G S I M V L G E G N L - - - - - - - - - - V Q E I D V I P S G S L A I D I A S G V G G Y P R<br />
M A R T T D D S K K A A P A A G T A D E A Q K Q K A L K M V L T Q I K R N F G E G A I M R L G - E N T - - - - - - - - - - R I R V E T V P S G A I T L D L A L G G G L P - R<br />
M K S E K R K A L E Q V L S Q I E R N F G K G A I M R L G - D A T - - - - - - - - - - Q M R V E T I P S G A I T L D L A L G G G Y P - K<br />
M K S E K R K A L D Q V L G Q I E R N F G K G A I M R L G - D A A - - - - - - - - - - Q M R V E T I P S G A I T L D L A M G G G Y P - K<br />
M A S T N I S D R E K A L N A A L A Q I E R S F G K G A I M R L G - D A T - - - - - - - - - - Q M R V E T I S T G A L T L D L A L G G G L P - K<br />
M S A I S N N P D K E K A L N L V L N Q I E R N F G K G A I M R L G - D A A - - - - - - - - - - Q M K V A T I P S G A L T L D Q A M G G G F P - R<br />
M A S K S D A I A P E K E K A L N L V L S Q I E R N F G K G A I M R L G - D A A - - - - - - - - - - R L R V E T I S T G A L T L D L A L G G G L P - K<br />
M A I N T D T S G K Q K A L T M V L N Q I E R S F G K G A I M R L G - D A T - - - - - - - - - - R M R V E T I S T G A L T L D L A L G G G L P - K<br />
M A I N T D T S G K Q K A L T M V L N Q I E R S F G K G A I M R L G - D A T - - - - - - - - - - R M R V E T I S S G A L T L D L A L G G G L P - K<br />
M E R V A V L F R R T C N S E N C S K G R R P L R E R P L A G L F F C S L P A P L S I P C L S L P G S N P G D F V P K V K T N Y I C N S C G Y Q S A K P L G R C P N C Q A W N S F E E E V P T A S T S G K S G R G G L G G Y G G V K G G K L T P L S T V G R R E E P R T P S G I P E L D R V L G G G L V - A<br />
Cab. thermophilum RecA<br />
Solibacter usitatus RecA<br />
Acidobacteria sp. Ellin345 RecA<br />
Dehalococcoides ethenogenes RecA<br />
Herpetosiphon aurantiacus RecA<br />
Chloroflexus aggregans RecA<br />
Cfx. aurantiacus J-10-fl RecA<br />
Roseiflexus sp. RS1 RecA<br />
Roseiflexus castenholzii RecA<br />
The. tengcongensis RecA<br />
The. ethanolicus RecA<br />
Clostridium novyi RecA<br />
Clostridium botulinum RecA<br />
Clostridium acetobutylicum RecA<br />
Pel. thermopropionicum RecA<br />
Car. hydrogeno<strong>for</strong>mans RecA<br />
Hb. modesticaldum RecA<br />
Desulfitobacterium hafniense RecA<br />
Streptococcus pyogenes RecA<br />
Staphylococcus aureus RecA<br />
Listeria monocytogenes RecA<br />
Bacillus subtilis RecA<br />
Bacillus cereus RecA<br />
Bacillus anthracis RecA<br />
Aquifex pyrophilus RecA<br />
Aquifex aeolicus RecA<br />
Deinococcus radiodurans RecA<br />
Thermus thermophilus RecA<br />
Thermus aquaticus RecA<br />
Corynebacterium glutamicum RecA<br />
Brevibacterium linens RecA<br />
Arthrobacter aurescens RecA<br />
Streptomyces coelicolor RecA<br />
Nocardia farcinica RecA<br />
Erythrobacter litoralis RecA<br />
Blastochloris viridis RecA<br />
Rhodopseudomonas palustris RecA<br />
Bradyrhizobium japonicum RecA<br />
Roseobacter denitrificans RecA<br />
Rhodobacter sphaeroides RecA<br />
Rhodobacter capsulatus RecA<br />
Shewanella oneidensis RecA<br />
Proteus vulgaris RecA<br />
Shigella flexneri RecA<br />
Escherichia coli RecA<br />
Halorhodospira halophila RecA<br />
Dechloromonas aromatica RecA<br />
Bordetella pertussis RecA<br />
Bordetella parapertussis RecA<br />
Stigmatella aurantiaca RecA<br />
Anaeromyxobacter sp. RecA<br />
Anaeromyxobacter dehalogens RecA<br />
Geobacter lovleyi RecA<br />
Geobacter sulfurreducens RecA<br />
Geobacter metallireducens RecA<br />
Campylobacter jejuni RecA<br />
Thiomicrospira denitrificans RecA<br />
Wolinella succinogenes RecA<br />
Helicobacter pylori RecA<br />
Chloroherpeton thalassium RecA<br />
Chlorobium ferooxidans RecA<br />
Pelodictyon luteolum RecA<br />
Chlorobium vibrio<strong>for</strong>me RecA<br />
Chlorobium chlorochromatii RecA<br />
Pld. phaeoclathrati<strong>for</strong>me RecA<br />
Prosthecochloris aesturarii RecA<br />
Chlorobium phaeobacteroides RecA<br />
Chlorobium limicola RecA<br />
Chlorobium phaeobacteroides BS1<br />
Chlorobium tepidum RecA<br />
Chlorobaculum parvum RecA<br />
Cytophaga hutchinsonii RecA<br />
Porphyromonas gingivalis RecA<br />
Bacteriodes thetaiotaomicron RecA<br />
Bacteriodes fragilis RecA<br />
Petrotoga mobilis RecA<br />
Thermotoga maritima RecA<br />
Thermosipho melanesiensis RecA<br />
Fervidobacterium nodosum RecA<br />
Gloeobacter violaceus RecA<br />
Synechococcus sp. JA-2-3B'a(2-13)<br />
Synechococcus sp. JA-3-3Ab RecA<br />
Synechocystis sp. PCC 6803 RecA<br />
Synechococcus sp. PCC 7002 RecA<br />
Synechococcus sp. PCC 6301 RecA<br />
Nostoc sp. PCC 7120 RecA<br />
Nostoc puncti<strong>for</strong>me PCC 73102 RecA<br />
Deinococcus radiodurans DR1105<br />
61 170<br />
60 169<br />
62 171<br />
59 168<br />
59 168<br />
1 108<br />
61 170<br />
61 170<br />
61 170<br />
58 167<br />
57 166<br />
58 167<br />
62 171<br />
58 167<br />
58 167<br />
58 167<br />
57 166<br />
60 169<br />
72 181<br />
58 167<br />
57 166<br />
57 165<br />
57 166<br />
57 166<br />
64 173<br />
61 170<br />
71 180<br />
58 167<br />
58 167<br />
71 180<br />
69 178<br />
60 169<br />
59 168<br />
61 170<br />
69 178<br />
57 166<br />
71 180<br />
70 179<br />
71 180<br />
71 180<br />
71 180<br />
60 169<br />
60 169<br />
60 169<br />
60 169<br />
60 169<br />
59 168<br />
67 176<br />
67 176<br />
62 171<br />
62 171<br />
62 171<br />
58 167<br />
59 168<br />
59 168<br />
58 167<br />
58 167<br />
60 169<br />
60 169<br />
72 181<br />
72 181<br />
71 180<br />
72 181<br />
73 182<br />
71 180<br />
71 180<br />
71 180<br />
71 180<br />
71 180<br />
72 181<br />
73 182<br />
67 176<br />
67 176<br />
73 182<br />
75 184<br />
65 174<br />
61 170<br />
61 170<br />
60 169<br />
75 184<br />
57 166<br />
57 166<br />
61 170<br />
62 171<br />
64 173<br />
62 171<br />
62 171<br />
150 299<br />
G R I V E I Y G P E S G G K T T L A L H V A A E A Q R T G G N V A F I D A E H A L D P G Y A A K L G V D I D N L F V S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S A A F D L V V V D S V A A L V P K A E L D G - - - - - D M G D A L P G L Q A<br />
G R I N E I F G P E S S G K T T I A L Q V V A E A Q K A G G I A A F I D V E H A L D P T Y A R K L G V D V D N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T A H L I A S G Q I D V L V V D S V A A L V P K S E L D G - - - - - E M G D S H M G V Q A<br />
G R V V E I F G P E S S G K T T I A L Q V I A E A Q K A G G M A A F V D A E H A L D P T Y A R K L G V D V E N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T E A L V S S K A I D I L V V D S V A A L V P K A E L D G - - - - - E M G D S H M G L Q A<br />
G R I S E I F G P E G S G K T T L A Q H I I A Q A Q K M G E K A A Y I D V E H A L D P K Y A S T C G V N L D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - E A L G I A E E L V R S T A I G V I V I D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q A<br />
G R V I E I Y G P E S S G K T T L T Q H I V A E T Q K M G G V A A F I D A E H A F D P V Y A A N C G V N V E D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E T L V R S G A I D V I V V D S V A A L V P R A E I E G - - - - - D M G D S H V G L Q A<br />
M I E I F G P E S S G K T T L A Q H I I A E A Q K M G G V G A F I D A E H A F D P V Y A A R C G V N I S D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E M L V R S N A V D V I V I D S V A A L V P R A E I E G - - - - - D M G D S M P G M Q A<br />
G R V V E I F G P E S S G K T T L A Q H I I A E A Q K M G G V C A F I D A E H A F D P V Y A A R C G V N I D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E M L V R S N A I D V I V I D S V A A L V P R A E I E G - - - - - E M G D S M P G M Q A<br />
G R V V E I Y G P E S S G K T T L A Q H I I A E A Q K M G G I A A F I D A E H A F D P V Y A A R C G V D V N N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S N A V D V V V V D S V A A L V P R A E I E G - - - - - D M G D S L P G L Q A<br />
G R V V E I Y G P E S S G K T T L A Q H I I A E A Q K M G G V A A F I D A E H A F D P V Y A K R C G V D V D N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E T L V R S N A V D V V V V D S V A A L V P R A E I E G - - - - - D M G D S L P G L Q A<br />
G R I I E I F G P E S S G K T T L A L H M I A E A Q K I G G T G A F I D A E H A L D P V Y A K N L G V N I D D L L V A Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A<br />
G R I I E I F G P E S S G K T T L A L H M I A E A Q K M G G T G A F I D A E H A L D P V Y A K N L G V N I E D L L V A Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I D G - - - - - E M G D S H V G L Q A<br />
G R I I E I F G P E S S G K T T V A L H I I A E A Q K S G G T A A F I D A E H A L D P V Y A K A L G V D I D N L I V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S G A I D V I V V D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A<br />
G R I I E I F G P E S S G K T T V A L H I L A E A Q K I G G A A A F I D A E H A L D P S Y A R N L G V D I D N L I V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T E A L V R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q A<br />
G R V V E I Y G P E S S G K T T V A L H I L A E A Q K K G G A A A F I D A E H A L D P Q Y A R A L G V D I D N L V V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A I D V I V V D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A<br />
G R V I E I F G P E S S G K T T V A L H V I A E A Q K M G G T A A F V D A E H A L D P V Y A K N L G V D I E N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q A<br />
G R V V E I F G P E S S G K T T V A L H I I A E A Q K M G G I A A F I D A E H A L D P V Y A Q K L G V D I E N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E S L V R S G A I D V I V I D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q A<br />
G R I V E V Y G P E S S G K T T V A L H I I A E A Q K M G G M A A F I D A E H A L D P V Y A R N L G V D I D N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S G A I D V V V I D S V A A L V P K A E I D G - - - - - E M G D A H V G L Q A<br />
G R V I E I Y G P E S S G K T T V A L H I A A E A Q K L G G V A A F I D A E H A L D P V Y A K A L G V N I D N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C E A L V R S G A I D V I I I D S V A A L V P R A E I E G - - - - - E M G D S H V G L H A<br />
G R I I E I Y G P E S S G K T T V A L H A V A Q A Q K E G G I A A F I D A E H A L D P A Y A A A L G V N I D E L L L S Q P D S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A G K L I D S G A V D L V V V D S V A A L V P R A E I D G - - - - - D I G D S H V G L Q A<br />
G R I I E I Y G P E S S G K T T V A L H A I A E V Q S N G G V A A F I D A E H A L D P E Y A Q A L G V D I D N L Y L S Q P D H - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A E A F V R S G A V D I V V V D S V A A L T P K A E I E G - - - - - E M G D T H V G L Q A<br />
G R I I E V Y G P E S S G K T T V A L H A I A E V Q A Q G G T A A F I D A E H A L D P A Y A K N L G V N I D E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D M L V I D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q A<br />
G R I I E V Y G P E S S G K T T V A L H A I A E V Q Q Q R - T S A F I D A E H A L D P V Y A Q K L G V N I E E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E A L V R S G A V D I V V V D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q A<br />
G R I I E I Y G P E S S G K T T V S L H A I A E V Q R Q G G Q A A F I D A E H A M D P V Y A Q K L G V N I D E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q A<br />
G R I I E I Y G P E S S G K T T V S L H A I A E V Q R Q G G Q A A F I D A E H A M D P V Y A Q K L G V N I D E L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q G L E I A E A L V R S G A V D I I V I D S V A A L V P K A E I E G - - - - - D M G D S H V G L Q A<br />
G R I T E I F G V E S S G K T T L A L H V I A E A Q K R G G V A V F I D A E H A L D P K Y A K K L G V D V D N L Y I S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E S L I N S G A V D V I V V D S V A A L V P K D E L E G - - - - - E M G E A Q V G K Q A<br />
G R I T E I F G V E S S G K T T L A L H V I A E A Q K R G G V A V F I D A E H A L D P K Y A K K L G V D V E N L Y I S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E S L I N S G A V D V I V V D S V A A L V P K D E L E G - - - - - E M G E A Q V G K Q A<br />
G R I T E I Y G P E S G G K T T L A L A I V A Q A Q K A G G T C A F I D A E H A L D P V Y A R A L G V N T D E L L V S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I M E L L V R S G A I D V V V V D S V A A L T P R A E I E G - - - - - D M G D S L P G L Q A<br />
G R I V E I Y G P E S G G K T T L G L T I I A Q A Q R R G G V A A F V D A E H A L D P L Y A Q R L G V Q V E D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E L L A R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D Q H V G L Q A<br />
G R I V E I Y G P E S G G K T T L A L T I I A Q A Q R R G G V A A F V D A E H A L D P L Y A Q R L G V Q V E D L L V S Q P E T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E L L A R S G A V D V I V V D S V A A L V P R A E I E G - - - - - E M G D Q H V G L Q A<br />
G R I V E V Y G P E S S G K T T V A L H A I A Q A Q K A G G I A A F I D A E H A L D P D Y A R K L G V D T D A L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G A I D I I V I D S V A A L T P K A E I E G - - - - - E M G D S H V G L Q A<br />
G R V V E I Y G P E S S G K T T V A L H A V A N A Q K T G G I A A F I D A E H A L D P E Y A K K L G V D T D Q L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L I R S G A L D V I V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A<br />
G R V V E I Y G P E S S G K T T V A L H A V A N A Q R A G G I A A F I D A E H A L D P D Y A A K L G V D T D A L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I M D M L V G S G S L D I V V I D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q A<br />
G R V V E V Y G P E S S G K T T L T L H A V A N A Q K A G G Q V A F V D A E H A L D P E Y A K K L G V D I D N L I L S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D M L V R S G A L D L I V I D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q A<br />
G R I V E I Y G P E S S G K T T V A L H A V A N A Q A A G G V A A F I D A E H A L D P D Y A R K L G V D T D A L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G A I D I I V I D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q A<br />
G R V I E V Y G P E S S G K T T L A L H A I A E A Q K A G G T A A F V D A E H A L D P V Y A K A L G V D I D E L I V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D T L V R S N A V D I L V V D S V A A L V P R A E I E G - - - - - E M G D S H V G L Q A<br />
G R V V E I F G P E S S G K T T L A L H T V A E A Q K K G G V C A F I D A E H A L D P L Y A R K L G V N L D D L L I S Q P D A - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D T L V R S G A I D V L V V D S V A A L V P R A E L E G - - - - - E M G D S Q P G M Q A<br />
G R I V E I Y G P E S S G K T T L A L H C V A E A Q K K G G I C A F V D A E H A L D P V Y A R K L G V N V D D L L I S Q P D H - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D T L V R S G A I D V L I V D S V A A L V P R A E L E G - - - - - E M G D A L P G L Q A<br />
G R V V E I Y G P E S S G K T T L A L H T V A E A Q K K G G I C A F I D A E H A L D P V Y A R K L G V N I D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D T L V R S G A V D V L V V D S V A A L V P K A E L E G - - - - - E M G D A L P G L Q A<br />
G R I V E I Y G P E S S G K T T L T L H C V A E Q Q K A G G V C A F V D A E H A L D P T Y A K K L G V D L D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D T L V R S G A V N M V I V D S V A A L T P K S E L E G - - - - - D M G D S S V G V Q A<br />
G R I I E I Y G P E S S G K T T L T L H V V A E E Q K K G G V C A F V D A E H A L D P Q Y A K K L G V N L D E L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D T L V R S G A V N L I V V D S V A A L T P K S E I E G - - - - - D M G D M Q M G S Q A<br />
G R I V E I Y G P E S S G K T T L T L H C I A E E Q K K G G V C A F V D A E H A L D P Y Y A K K L G V S L E D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D T L V R S G A V S L V V V D S V A A L T P K A E I E G - - - - - D M G D A T V G A Q A<br />
G R I V E I Y G P E S S G K T T L T L E V I A A A Q R E G K T C A F I D A E H A L D P I Y A K K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L T R S G A V D V I I V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A A<br />
G R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A K K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L T R S G A V D V I I V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A A<br />
G R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A R K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L A R S G A V D V I V V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A A<br />
G R I V E I Y G P E S S G K T T L T L Q V I A A A Q R E G K T C A F I D A E H A L D P I Y A R K L G V D I D N L L C S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I C D A L A R S G A V D V I V V D S V A A L T P K A E I E G - - - - - E I G D S H M G L A A<br />
G R V V E I Y G P E S S G K T T L T L H I I A E A Q K A G G A A A F V D A E H A L D P D Y A E A V G V D I N D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G G L D V V V V D S V A A L T P K A E I E G - - - - - E M G D S H V G L Q A<br />
G R V V E I Y G P E S S G K T T L C L Q V V A E M Q K L G G V A A F I D A E H A L D P Q Y A Q K L G V N V G D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A D M L V R S G S V D I I V V D S V A A L T P R A E I E G - - - - - E M G D Q M V G L H A<br />
G R V I E V Y G P E S S G K T T L T L Q V I A E M Q K L G G T C A F V D A E H A L D V Q Y A S K L G V N L T D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D A L V R S G S V D L I V I D S V A A L V P K A E I E G - - - - - E M G D S L P G L Q A<br />
G R V I E V Y G P E S S G K T T L T L Q V I A E M Q K L G G T C A F V D A E H A L D V Q Y A S K L G V N L T D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I T D A L V R S G S V D L I V I D S V A A L V P K A E I E G - - - - - E M G D S L P G L Q A<br />
G R I V E I F G P E S S G K T T L C L H I V A E A Q K R G G I C G Y I D A E H A L D V G Y A R K L G V R T D D L L L S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E M L V R S G A I D V L V V D S V A A L V P K A E L E G - - - - - E M G D A H M G V Q A<br />
G R I I E I Y G P E S S G K T T L A L H A V A E A Q K K G G I A A F V D A E H A L D V G Y A R K L G V R T D D L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E T L V R S G A I D V L V V D S V A A L V P K A E L E G - - - - - E M G D A H M G V Q A<br />
G R I I E I Y G P E S S G K T T L A L H A I A E A Q R R G G I A A F V D A E H A L D V G Y A R K L G V R T D D L L I S Q P D S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E T L V R S G A I D V L V V D S V A A L V P K A E L E G - - - - - E M G D A H M G V Q A<br />
G R V I E V Y G P E S S G K T T L A L H I V A E A Q K S G G I A A F V D A E H A L D I N Y A R K L G V K T D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E T L V R S G A I D V L V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A<br />
G R I I E I Y G P E S S G K T T L A L H I A A E A Q K M G G I A A F V D A E H A L D I G Y A R K L G V K T D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E M L V R S G A V D V L V I D S V A A L V P K A E I E G - - - - - E M G D S H M G L Q A<br />
G R V I E I Y G P E S S G K T T L A L H I A A E A Q K L G G I A A F V D A E H A L D I G Y A R K L G V R T D D L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E M L V R S G A V D V L V I D S V A A L V P K A E I E G - - - - - E M G D S H V G L Q A<br />
G R I I E I Y G P E S S G K T T L T L H I I A E C Q K A G G V C A F I D A E H A L D V K Y A K N L G V N T D D L Y V S Q P D F - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V E T I A R S G A V D L I V V D S V A A L T P K A E I E G - - - - - D M G D Q H V G L Q A<br />
G R V V E I Y G P E S S G K T T L A L Q I T A E C Q K A G G V C A F I D A E H A L D V G Y A K N L G V D V D N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L D I V E T I A R S G A I D L I V I D S V A A L T P K S E I E G - - - - - E M S D Q N V G V Q A<br />
G R I I E I Y G P E S S G K T T L A L Q V I A E C Q K K G G I C A F I D A E H A L D V S Y A K R L G V D A E N L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I L E T L T R S G A V D L I V V D S V A A L T P K S E I D G - - - - - D M G D Q H V G L Q A<br />
G R I I E I Y G P E S S G K T T L S L H I I A E C Q K N G G V C A F I D A E H A L D V H Y A K R L G V D T E N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I L E T I T R S G G I D L V V V D S V A A L T P K A E I D G - - - - - D M G D Q H V G L Q A<br />
G R I V E I Y G P E S S G K T T L A L H A I A E A Q K A G G I A A F V D A E H A F D Q S Y A R K L G I D I K S L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I T E T L V R S G A V D I I V V D S V A A L V P Q A E L E G - - - - - E M G D S Q M G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A L V D A E H A F D P S Y A R R L G V D I N S M L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H V I A E A Q K A G G I A A L V D A E H A F D P S Y A R K L G V D I N A M L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H V I A E A Q K A G G I A A I V D A E H A F D P S Y A R R L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V A V D S V A A L V P Q A E L E G - - - - - E M G D T V V G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I T A I V D A E H A F D P S Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S S M G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S N A I D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S N A I D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L T I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H V I A E A Q K E G G I A A I V D A E H A F D P T Y A R K L G V D I N A L L I S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L T I V E T L V R S G A V D V V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H A I A E A Q K G G G I A A M V D A E H A F D Q T Y A K K L G L D I G S L L V S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D I V V V D S V A A L V P Q A E L E G - - - - - E M G D S V M G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H A I A E A Q K N G G I A A L V D A E H A F D P T Y A R K L G V D I N A L L V S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D I I V I D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q A<br />
G R V T E I Y G P E S S G K T T L A L H A I A E A Q K E G G I A A L V D A E H A F D P T Y A R K L G V D I N S L L V S Q P E S - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L S I V E T L V R S G A V D I V V V D S V A A L V P Q A E L E G - - - - - E M G D S V V G L Q A<br />
G R I V E I Y G P E S S G K T T L S M H C I A E A Q K A G G I A A F I D A E H A F D K T Y A E K L G I D T T N L L I S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E H L I R S G A I D I I V I D S V A A L V P K A E I E G - - - - - E M G D S K M G L Q A<br />
G R I I E I Y G P E S S G K T T L A I H A I A E A Q K A G G L A A I I D A E H A F D R T Y A E K L G V N V D N L W I A Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E Q L I R S S A V D I I V I D S V A A L T P K A E I E G - - - - - E M G D N K V G L H A<br />
G R I I E I Y G P E S S G K T T L A I H A I A E A Q K A G G I A A F I D A E H A F D R F Y A A K L G V D V D N L W I S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E Q L I R S S A I D I I V I D S V A A L T P K A E I E G - - - - - D M G D N K V G L Q A<br />
G R I I E I Y G P E S S G K T T L A I H A I A E A Q K A G G I A A F I D A E H A F D R F Y A A K L G V D V D N L F I S Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I A E Q L I R S S A I D I I V V D S V A A L T P K A E I E G - - - - - D M G D N K V G L Q A<br />
G R I I E I Y G N E S S G K T T L A L H S L A E V Q K L N G I V A F V D A E H A L D V E Y A R K L G V D V D K L I V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D S L V R S N I V D L I V V D S V A A L V P K A E I E G - - - - - A M G D S H M G L Q A<br />
G R I V E I F G Q E S S G K T T L A L H A I A E A Q K M G G V A A F I D A E H A L D P V Y A K N L G V D L K S L L I S Q P D H - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D E L V R S G V V D L I V V D S V A A L V P R A E I E G - - - - - A M G D M Q V G L Q A<br />
G R I V E I Y G P E S S G K T T I A L H A I A S A Q K N G G I A A F V D A E H A L D P V Y A K N L G V D V E N L L I A Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L E I V D E L V R S N A V D L V V I D S V A A L V P R A E I E G - - - - - S M G D V Q V G L Q A<br />
G R I I E I Y G P E S S G K T T I A L H A I A S V Q K L G G I A A F V D A E H A L D L N Y A K N L G I D L S E L L V S Q P D Y - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - Q A L D I V D E L V R S N A V D L I V V D S V A A L V P R A E I E G - - - - - A M G D A Q I G L Q A<br />
G R V I E I Y G P E S S G K T T L A L H A I A E I Q K T G G V A A F V D A E H A L D P A Y A K V L G V N V D D L I I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - M A M E I V D Q L V R S A A I D V I V I D S V A A L V P R A E I E G - - - - - E M G D A H V G L Q A<br />
G R I I E V F G P E S S G K T S I A L H A I A E V Q K Q G G T A A F V D A E H A L D P T Y A A A L G V D I Q N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - A A L E I V D Q L V R S A A V D I V V V D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q A<br />
G R I I E V F G P E S S G K T S V A L H A I A E V Q K R G G T A A F V D A E H A L D P A Y A A A L G V D I Q N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - A A L E I V D Q L V R S A A V D I V V V D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q A<br />
G R I V E I Y G P E S S G K T T L A L H A V A A T Q Q A G G V A A F V D A E H A L D P V Y S K A L G V D I D N L L V A Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I V D Q L V R S T A V D I I V V D S V A A L V P R A E I E G - - - - - E M G D T S V G S Q A<br />
G R I V E I Y G P E S S G K T T V A L H A I A E V Q K A G G V A A F I D A E H A L D P T Y S A A L G V D I E N L L V A Q P D N - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I A D Q L V R S A A V D L I V I D S V A A L V P R A E I E G - - - - - E M G D V Q V G L Q A<br />
G R I I E V Y G P E S S G K T T L T L H A I A E V Q K Q G G I A A F V D A E H A L D P V Y A T S V G V D I D N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - M A L E I V D Q L V R S A A V D I V V I D S V A A L V P R A E I E G - - - - - E M G D A Q V G L Q A<br />
G R V I E I Y G P E S S G K T T V A L H A I A E V Q K E G G I A A F V D A E H A L D P T Y A S A L G V D I Q N L L V S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I V D Q L V R S A A V D I V V I D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q A<br />
G R V I E I Y G P E S S G K T T V A L H A L A E V Q R N G G I A A F V D A E H A L D P T Y A A A L G V D I D N L L I S Q P D T - - - - - - - - - - - - - - - - - - - - - - - - - - - - G E - - - - - - - S A L E I V D Q L V R S A A V D I V V I D S V A A L V P R A E I E G - - - - - D M G D A H V G L Q A<br />
G G V T L I G G E P G I G K S T L L L Q V A D K V A S R G G T V L Y V A G E E S L E Q I R L R A D R L G V A A D L Q M T R D T R A E H I A A L L E E H K P A L C I V D S I Q T V T V E G E G A P G G V A Q V R D G T A M L T R A A K E T G T A T V L V G H V T K D G T V A G P K V M E H I V D T T V F L E T<br />
Cab. thermophilum RecA<br />
Solibacter usitatus RecA<br />
Acidobacteria sp. Ellin345 RecA<br />
Dehalococcoides ethenogenes RecA<br />
Herpetosiphon aurantiacus RecA<br />
Chloroflexus aggregans RecA<br />
Cfx. aurantiacus J-10-fl RecA<br />
Roseiflexus sp. RS1 RecA<br />
Roseiflexus castenholzii RecA<br />
The. tengcongensis RecA<br />
The. ethanolicus RecA<br />
Clostridium novyi RecA<br />
Clostridium botulinum RecA<br />
Clostridium acetobutylicum RecA<br />
Pel. thermopropionicum RecA<br />
Car. hydrogeno<strong>for</strong>mans RecA<br />
Hb. modesticaldum RecA<br />
Desulfitobacterium hafniense RecA<br />
Streptococcus pyogenes RecA<br />
Staphylococcus aureus RecA<br />
Listeria monocytogenes RecA<br />
Bacillus subtilis RecA<br />
Bacillus cereus RecA<br />
Bacillus anthracis RecA<br />
Aquifex pyrophilus RecA<br />
Aquifex aeolicus RecA<br />
Deinococcus radiodurans RecA<br />
Thermus thermophilus RecA<br />
Thermus aquaticus RecA<br />
Corynebacterium glutamicum RecA<br />
Brevibacterium linens RecA<br />
Arthrobacter aurescens RecA<br />
Streptomyces coelicolor RecA<br />
Nocardia farcinica RecA<br />
Erythrobacter litoralis RecA<br />
Blastochloris viridis RecA<br />
Rhodopseudomonas palustris RecA<br />
Bradyrhizobium japonicum RecA<br />
Roseobacter denitrificans RecA<br />
Rhodobacter sphaeroides RecA<br />
Rhodobacter capsulatus RecA<br />
Shewanella oneidensis RecA<br />
Proteus vulgaris RecA<br />
Shigella flexneri RecA<br />
Escherichia coli RecA<br />
Halorhodospira halophila RecA<br />
Dechloromonas aromatica RecA<br />
Bordetella pertussis RecA<br />
Bordetella parapertussis RecA<br />
Stigmatella aurantiaca RecA<br />
Anaeromyxobacter sp. RecA<br />
Anaeromyxobacter dehalogens RecA<br />
Geobacter lovleyi RecA<br />
Geobacter sulfurreducens RecA<br />
Geobacter metallireducens RecA<br />
Campylobacter jejuni RecA<br />
Thiomicrospira denitrificans RecA<br />
Wolinella succinogenes RecA<br />
Helicobacter pylori RecA<br />
Chloroherpeton thalassium RecA<br />
Chlorobium ferooxidans RecA<br />
Pelodictyon luteolum RecA<br />
Chlorobium vibrio<strong>for</strong>me RecA<br />
Chlorobium chlorochromatii RecA<br />
Pld. phaeoclathrati<strong>for</strong>me RecA<br />
Prosthecochloris aesturarii RecA<br />
Chlorobium phaeobacteroides RecA<br />
Chlorobium limicola RecA<br />
Chlorobium phaeobacteroides BS1<br />
Chlorobium tepidum RecA<br />
Chlorobaculum parvum RecA<br />
Cytophaga hutchinsonii RecA<br />
Porphyromonas gingivalis RecA<br />
Bacteriodes thetaiotaomicron RecA<br />
Bacteriodes fragilis RecA<br />
Petrotoga mobilis RecA<br />
Thermotoga maritima RecA<br />
Thermosipho melanesiensis RecA<br />
Fervidobacterium nodosum RecA<br />
Gloeobacter violaceus RecA<br />
Synechococcus sp. JA-2-3B'a(2-13)<br />
Synechococcus sp. JA-3-3Ab RecA<br />
Synechocystis sp. PCC 6803 RecA<br />
Synechococcus sp. PCC 7002 RecA<br />
Synechococcus sp. PCC 6301 RecA<br />
Nostoc sp. PCC 7120 RecA<br />
Nostoc puncti<strong>for</strong>me PCC 73102 RecA<br />
Deinococcus radiodurans DR1105<br />
171 305<br />
170 303<br />
172 305<br />
169 302<br />
169 302<br />
109 242<br />
171 304<br />
171 304<br />
171 304<br />
168 301<br />
167 300<br />
168 301<br />
172 305<br />
168 301<br />
168 301<br />
168 301<br />
167 300<br />
170 303<br />
182 319<br />
168 301<br />
167 300<br />
166 280<br />
167 300<br />
167 300<br />
174 307<br />
171 304<br />
181 315<br />
168 302<br />
168 302<br />
181 314<br />
179 312<br />
170 303<br />
169 302<br />
171 304<br />
179 312<br />
167 300<br />
181 314<br />
180 313<br />
181 314<br />
181 314<br />
181 314<br />
170 303<br />
170 303<br />
170 303<br />
170 303<br />
170 303<br />
169 302<br />
177 310<br />
177 310<br />
172 305<br />
172 305<br />
172 305<br />
168 301<br />
169 302<br />
169 302<br />
168 302<br />
168 302<br />
170 304<br />
170 304<br />
182 315<br />
182 315<br />
181 314<br />
182 315<br />
183 316<br />
181 314<br />
181 314<br />
181 314<br />
181 314<br />
181 314<br />
182 315<br />
183 316<br />
177 311<br />
177 310<br />
183 316<br />
185 318<br />
175 313<br />
171 309<br />
171 309<br />
170 308<br />
185 318<br />
167 301<br />
167 301<br />
171 305<br />
172 306<br />
174 307<br />
172 305<br />
172 305<br />
300 446<br />
R L M S Q A L R K L T A V A N R T N T C L I F I N Q I R E K I G - - - - - - - V T W G S P E T T T G G R A L K F Y A S V R I D I R R T A S I K D G - E E - - - V I G S R T K A K I A K N K L A P P F K E V E F D I I Y G K G I C R E G D L L D L G V A H K L I E K S G T W F S L K S - - - - G E R L G Q G R<br />
R L M S Q A L R K L T G S V N K S R T C L I F I N Q I R E K I G - - - - - - - V M F G N P E T T T G G R A L K F Y S T V R L D I R R I G A I K D G - D A - - - V V G N R T K V K V V K N K V A S P F R E A E F D I I Y G E G V S K E G D L L D L G V A Q N L V E K S G S W F S Y K - - - - - G D R I G Q G R<br />
R L M S Q A L R K L T G T V S K S G T C L I F I N Q I R E K I G - - - - - - - V M F G N P E T T T G G R A L K F Y S S V R V D I R R I A A I K D G - D T - - - V I G S R T K V K V V K N K T A A P F R E A E F D I V Y G E G I S K E G D M L D L A V E A N I V E K S G S W F S Y K - - - - - T E R I G Q G R<br />
R L M S Q A L R K L T A S I G Q T R T A V I F I N Q L R E K V G - - - - - - - V M F G N P E V T P G G R A L K F Y S S V R I D L R R I E T I K Q G - T V - - - A I G T R V R A K V V K N K V A P P F R T A E F D I M F D S G I S R E G N L I D L G V T S E V I R K A G A F F S Y G - - - - - D I R L G Q G R<br />
R L M S Q A L R K L S G A V S K S R T A L I F I N Q L R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R K V E S I K Q G - Q D - - - V T G A R A R V K V V K N K V A P P F R Q A E F D I M Y N E G I S R E G N I V D V G V T M E I L R K S G A W F Y L G - - - - - D D R L G Q G R<br />
R L M S Q A L R K L S G A I S K S R A V V I F I N Q L R M K I G - - - - - - - V M F G S P E T T T G G Q A L K F Y A S V R L D I R R I E T L K Q G - Q E - - - A I G S R V R V K V I K N K V A P P F R Q A E F D I L A N E G I S R E G N I I D I G T E L G I I R K S G A W F Y L G - - - - - E D R L G Q G R<br />
R L M S Q A L R K L S G A I S K S R T V V I F I N Q L R M K I G - - - - - - - V M L G T P E T T T G G Q A L K F Y T S V R L D I R R V E T L K Q G - Q E - - - A I G S R V R V K V I K N K V A P P Y R Q A E F D I L A N E G I S R E G N I I D I G T E L G I I R K S G A W F Y L G - - - - - E D R L G Q G R<br />
R L M S Q A L R K L S G A I S K S R A V V I F L N Q L R L K I G - - - - - - - V M F G S P E T T T G G Q A L K F Y A S V R M D I R R I E T L K N G - Q E - - - T I G S R T R V K V V K N K V A P P F R Q A E F D I M H N E G I S R A G N I L D V G V E L D I I R K S G A W F Y L G - - - - - D D R L G Q G R<br />
R L M S Q A L R K L S G A I S K S R V V V I F L N Q L R L K I G - - - - - - - V M F G S P E T T T G G Q A L K F Y A S V R M D I R R I E T L K N G - Q E - - - T I G S R T R V K V V K N K V A P P F R Q A E F D I M H N E G I S R A G N I L D V G V E L D I I R K S G A W F Y L G - - - - - E D R L G Q G R<br />
R L M S Q A L R K L A G V T S K S K S I V V F I N Q L R E K V G - - - - - - - V M F G N P E T T P G G R A L K F Y A T I R L D V R K V D N I K Q G - N E - - - I V G S R T R V K V V K N K I A P P F K Q A E F D I M Y G E G I S R E G S I L D L G T A L D I I E K S G S W Y S Y K - - - - - D I K L G Q E E<br />
R L M S Q A L R K L A G V T S K S K T I V V F I N Q L R E K V G - - - - - - - V L F G N P E T T P G G R A L K F Y A T I R L D V R K V D A I K Q G - N E - - - I I G N R T R V K V V K N K I A P P F K Q A E F D I M Y G E G I S R E G S I L D I G T S I D I I E K S G A W Y S Y G - - - - - D I R L G Q G R<br />
R L M S Q A L R K L T G S I N K S K C A T I F I N Q L R E K V G - - - - - - - I M F G N P E T T P G G R A L K F Y S S V R L D V R K I E T I K Q G - D E - - - F L G S R T R V K V V K N K V A P P F K Q A E F D I M Y G T G I S F E G N V L D V G V D N E I I Q K S G S W F S Y N - - - - - D T R L G Q G R<br />
R L M S Q A L R K L A G S I N K S K C V A I F I N Q L R E K V G - - - - - - - I M F G N P E T T P G G R A L K F Y S S V R L D V R R I D S I K Q G - D Q - - - I L G N R T R V K I N K N K V A P P F K M A E F D I M Y N E G I S K V G N I L D V G V R E E L V D K S G S W F S Y K - - - - - D T R L G Q G R<br />
R L M S Q A L R K L T G S I N K S K C I A I F I N Q L R E K V G - - - - - - - I M F G N P E T T P G G R A L K F Y A S V R M D I R K I D T I K Q G - E E - - - M I G N R V R V K V V K N K V A P P F K Q A E F D I M Y N E G I S R E G N I V D V G V K E N I I Q K S G A W F S Y G - - - - - D I R L G Q G R<br />
R L M S Q A L R K L T G A I S K S R T T V I F I N Q I R E K V G - - - - - - - V V Y G S P E T T T G G R A L K F Y A S I R L E V R K Q E N I K Q G - T D - - - I I G S R T R V K V V K N K V A P P F K Q A D F D I M Y G T G I S K E G S I L D M A T E L N I V E K S G A W Y S F G - - - - - E E R L G Q G R<br />
R L M S Q A L R K L T G A I S K S K T V A I F I N Q M R E K V G - - - - - - - V M F G N P E T T P G G R A L K F Y A S I R M D V R K I D V I K Q G - N D - - - I I G S R T R V K I V K N K V A P P F K Q A D F D I M Y G E G I S K E G S L L D V A V E L K I V Q K V G S W Y S Y N - - - - - D E R L G Q G R<br />
R L M S Q A L R K L T G V V S K S K T C C L F I N Q I R E K V G - - - - - - - V M F G N P E T T P G G R A L K F Y S S V R L E V R K V D T L K Q G - S D - - - M I G S R T R V K V V K N K V A P P F K V A D F D I M Y G E G I S R E G S L V D L A V D M G I V E K S G A W Y S Y K - - - - - G D R L G Q G R<br />
R L M S Q A L R K L T G A I S K S N T C V I F I N Q I R E K V G - - - - - - - I M F G N P E T T T G G R A L K F Y A S V R L E V R R A D T L K Q G - Q D - - - M I G N R T K V K V V K N K V A P P F K Q A E F D I M Y G E G I S R E G S M V D V G A E L D I I N K S G A W Y S Y K - - - - - G E R L G Q G R<br />
R M M S Q A M R K L S A S I N K T K T I A I F I N Q L R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y A S V R L D V R G T T Q I K G T G D Q K D S S I G K E T K I K V V K N K V A P P F K V A E V E I M Y G E G I S R T G E L V K I A S D L D I I Q K A G A W F S Y N - - - - - G E K I G Q G S<br />
R L M S Q A L R K L S G A I S K S N T T A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S S V R L E V R R A E Q L K Q G Q E - - - - I V G N R T K I K V V K N K V A P P F R V A E V D I M Y G Q G I S K E G E L I D L G V E N D I V D K S G A W Y S Y N - - - - - G E R M G Q G K<br />
R L M S Q A L R K L S G V I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E I T P G G R A L K F Y S T V R L E V R R A E Q L K Q G T D - - - - V M G N K T K I K V V K N K V A P P F R I A E V D I M Y G E G I S R E G E L V D M A A E V D V I N K S G S W Y S Y K - - - - - E E R I G Q G R<br />
R L M S Q A L R K L S G A I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S S V R L E V R R A E Q L K Q G N D - - - - V M G N K T K I K V V K N K V A P P F R T A E V D I M Y G E G I S K E G E I I D L G T E L D I<br />
R L M S Q A L R K L S G A I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S T V R L E V R R A E Q L K Q G N D - - - - I V G N K T K V K V V K N K V A P P F R V A E V D I M Y G E G I S R E G E I L D M A S E L D I V Q K S G A W Y S Y N - - - - - E E R L G Q G R<br />
R L M S Q A L R K L S G A I N K S K T I A I F I N Q I R E K V G V - - - - - - - M F G N P E T T P G G R A L K F Y S T V R L E V R R A E Q L K Q G N D - - - - I V G N K T K V K V V K N K V A P P F R V A E V D I M Y G E G I S R E G E I L D M A S E L D I V Q K S G A W Y S Y N - - - - - E E R L G Q G R<br />
R L M S Q A L R K L K G A V H R S N T A L I F I N Q I R E K I G - - - - - - - V M F G N P E T T P G G R A L K F F S D M R L E V R R L G - D V K E G G E K K - - G - Y R V K V R V V K N K L A P P F Q E A E F D V I Y G E G I C R I C D I I D T A A N L G V I T K S G S W Y S Y G - - - - - E K R L G Q G R<br />
R L M S Q A L R K L K G A V H K S N T A L I F I N Q I R E K I G - - - - - - - V M F G N P E T T P G G R A L K F F S D M R L E V R R L G - D V K E G G E K K - - G - Y R V K V R V V K N K L A P P F Q E A E F D I I Y G E G I C K L C D L I E V A T N L G I F T K S G S W Y S Y G - - - - - D K R L G Q G K<br />
R L M S Q A L R K L T A I L S K T G T A A I F I N Q V R E K I G - - - - - - - V M Y G N P E T T T G G R A L K F Y A S V R L D V R K I G Q P T K V G N D A V - - A - N T V K I K T V K N K V A A P F K E V E L A L V Y G K G F D Q L S D L V G L A A D M D I I K K A G S F Y S Y G - - - - - D E R I G Q G K<br />
R L M S Q A L R K L T A V L A K S N T A A I F I N Q V R E K V G - - - - - - - V T Y G N P E T T P G G R A L K F Y A S V R L D V R K S G Q P I K V G N E A V - - A - V K V R V K V V K N K L A P P F R E A E L E I Y F G R G L D P V A D L V N V A V A A G V I E K A G S W F S Y G - - - - - E L R L G Q G K<br />
R L M S Q A L R K L T A V L A K S N T A A I F I N Q V R E K V G - - - - - - - V T Y G N P E T T P G G R A L K F Y A S V R L D V R K S G Q P I K V G N E A V - - G - V K V R V K V V K N K L A P P F R E A E L E I Y F G R G L D P V A D L V N V A V A A G V I E K A G S W F S Y G - - - - - E L R L G Q G K<br />
R L M S Q A L R K M T G A L Y N S G T T A I F I N Q L R E K I G - - - - - - - V M F G S P E T T T G G K A L K F Y A S V R C D I R R I Q T L K D G - Q D - - - A I G N R T R L K V V K N K V S P P F K I A E F D I M Y G E G I S R E S S V I D L A V D N G I V K K S G S W F T Y E - - - - - G E Q L G Q G K<br />
R L M S Q A L R K I A G A L S Q S K T T A I F I N Q L R E K V G - - - - - - - V F F G S P E T T S G G K A L K F Y A S V R I D V R R I E T L K E G - Q D - - - A V G N R T R A K I V K N K I A P P F K Q A E F D I I Y G Q G I S R E G S L I D M G V D N G I I R K S G S W F T Y D - - - - - G D Q L G Q G K<br />
R L M S Q A L R K I T G R L S Q T K T T A I F I N Q L R E K I G - - - - - - - V F F G S P E T T T G G K A L K F Y A S V R I D V R R I Q T L K E G - A D - - - S V G N R T K A K I V K N K M A P P F K I A E F D I I Y G Q G I S R E G G I I D M G V E H G I I K K S G S W F T Y D - - - - - G D Q L G Q G M<br />
R L M S Q A L R K I T S A L N Q S K T T A I F I N Q L R E K I G - - - - - - - V M F G S P E T T T G G R A L K F Y A S V R L D I R R I E T L K D G - T D - - - A V G N R T R V K V V K N K V A P P F K Q A E F D I L Y G Q G I S R E G G L I D M G V E N G F V R K A G A W Y T Y E - - - - - G D Q L G Q G K<br />
R L M S Q A L R K M T S A L N N S G T T A I F I N Q L R E K I G - - - - - - - V M F G S P E T T T G G K A L K F Y A S V R L D V R R I E T L K D G - S D - - - A V G N R T R V K V V K N K V S P P F K Q A E F D I L Y G Q G I S K E G S L I D M G V E Q G F I R K S G S W Y T Y E - - - - - G D Q L G Q G K<br />
R L M S Q S L R K L T G S I N R S K C M V I F I N Q L R M K I G - - - - - - - V M Y G N P E T T T G G N A L K F Y A S V R L D I R R T G Q I K D R - D E - - - V V G N S T R V K V V K N K V A P P F K Q V E F D I M Y G E G I S K I G E I L D L G V K A G I V E K S G S W F S Y D - - - - - S I R I G Q G R<br />
R L M S Q A L R K L T A S I S R S N C M V I F I N Q I R M K I G - - - - - - - V M Y G S P E T T T G G N A L K F Y A S V R L D I R R I G A I K E R - D E - - - V V G N Q T R V K V V K N K L A P P F K Q V E F D I M Y G E G I S K A G E L I D L G V K A G V V D K S G S W F S H D - - - - - S Q R I G Q G R<br />
R L M S Q A L R K L T A S I N K S N T M V I F I N Q I R M K I G - - - - - - - V M Y G S P E T T T G G N A L K F Y A S V R L D I R R I G Q I K E R - D E - - - V V G N Q T R V K V V K N K L A P P F K Q V E F D I M Y G E G V S K M G E I L D L G V K A G I V E K S G A W F S H D - - - - - S Q R L G Q G R<br />
R L M S Q A L R K L T A S I N K S H T M V I F I N Q I R M K I G - - - - - - - V M Y G S P E T T T G G N A L K F Y A S V R L D I R R I G A I K E R - D E - - - V V G N T T R V K V V K N K L A P P F K Q V E F D I M Y G E G V S K M G E I L D L G V K A G I V E K S G A W F S Y D - - - - - S Q R L G Q G R<br />
R L M S Q A M R K L T G S I S R S N C M V I F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y S S V R L D I R R I G A L K D R - D E - - - V V G N H T R V K V V K N K V A A P F K Q V E F D I M Y G E G I S K M G E L L D L G V A A G V V D K S G S W F S Y G - - - - - D E R I G Q G R<br />
R L M S Q A M R K L T A S I G R S N C M V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R T G A I K D R - D N - - - V I G N T T K V K V V K N K V A P P F R E V E F D I M Y G E G I S K T G E L V D L G V K A G V V E K S G S W Y S Y G - - - - - D E R I G Q G R<br />
R L M S Q A M R K L T A S I G R S N C M V I F I N Q I R M K I G - - - - - - - V M F G S P E T T S G G N A L K F Y A S V R L D I R R T G A I K D R - D E - - - V I G N Q T R V K V V K N K V A P P F R E V E F D I L Y G E G I S K V G E L V D L G V K A G V V A K S G A W Y S Y G - - - - - D E R I G Q G R<br />
R M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R T G A I K E G - D E - - - V V G N E T R V K V V K N K V A A P F K Q A E F Q I L Y G Q G I N R T G E L V D L G V A H K L I E K A G A W Y S Y K - - - - - G D K I G Q G R<br />
R M M S Q A M R K L A G N L K Q A N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R T G A I K D G - E E - - - V V G S E T R V K V V K N K V A A P F K Q A E F Q I L Y G E G I N I H G E L V D L G V K H K L I E K A G A W Y S Y N - - - - - G D K I G Q G K<br />
R M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R I G A V K E G - E N - - - V V G S E T R V K V V K N K I A A P F K Q A E F Q I L Y G E G I N F Y G E L V D L G V K E K L I E K A G A W Y S Y K - - - - - G E K I G Q G K<br />
R M M S Q A M R K L A G N L K Q S N T L L I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R R I G A V K E G - E N - - - V V G S E T R V K V V K N K I A A P F K Q A E F Q I L Y G E G I N F Y G E L V D L G V K E K L I E K A G A W Y S Y K - - - - - G E K I G Q G K<br />
R L M S Q A L R K L T G S I Q R S N T L V V F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y S S V R M D I R R L G A I K K G - D E - - - V L G N E T R V K V V K N K M A P P F K Q A E F E I L Y G E G I S R E G E I I D M G V A Q G F V D K S G A W Y S Y N - - - - - G D R I G Q G K<br />
R L M S Q A L R K L T A N I K K T N T L V I F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y A S V R L D I R R I G A I K K G - D E - - - V I G S E T K V K V V K N K V A P P F R E A I F D I L Y G E G I S R Q G E I V E M G V A H K L V D K S G A W Y A Y K - - - - - G E K I G Q G K<br />
R L M S Q A L R K L T A T I K R T N C M V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y S S V R L D I R R I G A I K K G - D E - - - V V G N E T R V K V V K N K V A P P F K Q A E F D I M Y G S G I S R E G E I I D L G V Q A N V V D K S G A W Y S Y S - - - - - G N R I G Q G K<br />
R L M S Q A L R K L T A T I K R T N C M V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y S S V R L D I R R I G A I K K G - D E - - - V V G N E T R V K V V K N K V A P P F K Q A E F D I M Y G S G I S R E G E I I D L G V Q A N V V D K S G A W Y S Y S - - - - - G N R I G Q G K<br />
R L M S Q A L R K L T G T I S K S Q T C V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S Q R L D I R R I G A I K N G - E N - - - V V G S R T R V K V V K N K V A P P F K E V E F D I M Y G T G I S R E G D L I D L A S N E N I V E K S G S W F A F K - - - - - G E R I G Q G R<br />
R L M S Q A L R K L T G T I S K S S T I V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A T Q R L D I R R I G A I K D G - D Q - - - V I G N R T R V K V V K N K V A P P F K E V E F D I M Y G H G I S R E G D L L D L G S N E N I V E K S G T W F S F G - - - - - G E R I G Q G R<br />
R L M S Q A L R K L T G T I S K S S T I V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S Q R L D I R R I G A I K D G - D S - - - V I G N R T R V K V V K N K V A P P F K E V E F D I M Y G H G I S R E G D V L D L A S N E G I V E K S G T W F S F G - - - - - G E R I G Q G R<br />
R L M S Q A L R K L T G I I S K S N C C V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R K I A T L K Q G - D Q - - - V I G S R T R V K V V K N K V A P P F K E A E F D I L Y G E G I S R T G D V L D L A V D R N I I D K S G A W F S Y N - - - - - K E R I G Q G R<br />
R L M S Q A L R K L T G I I S K S N C C V I F I N Q I R M K I G - - - - - - - V M F G N P E T T T G G N A L K F Y A S V R L D I R K I A S L K Q G - Q D - - - V I G S R T K V K V V K N K V A P P F K E V E F D I Y Y G E G I S R E G D I L D L A V E K G I V D K S G A W F S Y G - - - - - G D R I G Q G R<br />
R L M S Q A L R K L T G I I S K S N C C V I F I N Q I R M K I G - - - - - - - V M F G S P E T T T G G N A L K F Y A S V R L D I R K I A T L K Q G - D A - - - V I G S R T K V K V V K N K V A P P F R E V E F D I Y Y G E G I S R L G D L L D L A V D R K I I D K S G A W F S Y G - - - - - S D R I G Q G R<br />
R L M S Q A L R K L T G I V H K M N T T V I F I N Q I R M K I G - - - - - - A M G Y G T P E T T T G G N A L K F Y A S V R L D V R K V A T L K Q N - E E - - - P I G N R V K V K V V K N K V A P P F R Q A E F D V M F G E G L S R E G E L I D Y G V K L D I V D K S G A W F S Y K - - - - - D K K L G Q G R<br />
R L M S K A L R K L T G I L H K M N C T V I F I N Q I R M K I G - - - - - - M M G Y G S P E T T T G G N A L K F Y A S V R I D V R R I A S L K Q G - E S - - - Q I G N R V K A K V I K N K V A P P F R Q A E F D I M F G E G I S K E G E L V D Y G V K L D I I D K S G A W F S Y G - - - - - E T K L G Q G R<br />
R L M S Q A L R K V T G V L H K M N T T V I F I N Q I R M K I
Figure 10S<br />
100<br />
100<br />
63<br />
62<br />
99<br />
76<br />
100<br />
53<br />
100<br />
93<br />
Chl. vibrio<strong>for</strong>me<br />
Pld. luteolum<br />
Chl. phaeobacteroides BS1<br />
Ptc. aestuarii<br />
Chl. chlorochromatii<br />
Pld. phaeoclathrati<strong>for</strong>me<br />
Chl. limicola<br />
Chl. phaeobacteroides<br />
Chl. tepidum<br />
Cab. thermophilum<br />
Cfx. aurantiacus<br />
Cfx. aggregans<br />
Rhb. sphaeroides CrtF<br />
Rps. palustris CrtF<br />
Rvx. gelatinosus CrtF<br />
A. thaliana COMT
Cab. thermophilum BchU<br />
Cfx. aurantiacus BchU<br />
Cfx. aggregans BchU<br />
Chl. vibrio<strong>for</strong>me BchU<br />
Pld. luteolum BchU<br />
Chl. phaeobacteroides BS1 BchU<br />
Ptc. aestuarii BchU<br />
Chl. chlorochromatii BchU<br />
Pld. phaeoclathrati<strong>for</strong>me BchU<br />
Chl. limicola BchU<br />
Chl. phaeobacteroides BchU<br />
Chl. tepidum BchU<br />
Rps. sphaeroides CrtF<br />
Rps. palustris CrtF<br />
Rvx. gelatinosus CrtF<br />
A. thaliana ComT<br />
1 42<br />
1 44<br />
1 44<br />
1 51<br />
1 28<br />
1 28<br />
1 28<br />
1 29<br />
1 28<br />
1 28<br />
1 28<br />
1 28<br />
1 62<br />
1 58<br />
1 70<br />
1 51<br />
M S N P V S T A A E A L S T V R E E V L Y E Q Y L R - - - - A C D M T Y R G T V D M F V L K<br />
M T T V P S V D A T K T N G T D L T Q H D L F A A T N R - - - - A Y D M V F K G T V D F F V I K<br />
M T T A P S I D A T T T A G T A L E Q Q E L F A A T N R - - - - A Y D M V F K G T V D F F V I K<br />
M L P E P A A C E R L F L S I N C S Q Y M T M M N D I Q L L Q Q N E R - - - - A N E L I F K G L V E F G C F K<br />
M N E I A L L K Q N H R - - - - A N E L I F K G L V E F G C F K<br />
M N D N E L L K R N H R - - - - A N E I L F K G L V E F G C F K<br />
M N D N E L L K Q N H R - - - - A N E I V F K G L V E F G C F K<br />
M M N T N E L L N Y N H R - - - - A N E L V F K G L V E F G C I K<br />
M D A N E L L K Q N H R - - - - A N E L I F K G L V E F G C F K<br />
M N D L E L L K Q N H R - - - - A N E L I F K G L V E F G C F K<br />
M N D I E L L Q Q N H R - - - - A N E L C F K G L V E F G C F K<br />
M S N N D L L N Y Y H R - - - - A N E L V F K G L I E F S C M K<br />
M S A L R P P A R G G L G L S R L G V R L A G S R R F Q L I A E R V P F L R R I G R R E G E E L F D I V A G F V H S Q V L Y<br />
M V S L S L R D R L L G W R D S I L S N P R F Q R F A A V F P L M R P V A R R R A V A M F D L V A G F V Y S Q I L L<br />
M D R A E T R P E A G P R R S P T L A E R F R D W K H R T I A R P G F Q K W A A A F P L T R P V A R K H A R E L F D L L A G F V Y S Q I L L<br />
M I S L Q T S G G S S E E E D M L L A I Q L G G L N F V P Y I V K T A R - - - - - E L D L F E I M A K A R P L G<br />
Cab. thermophilum BchU<br />
Cfx. aurantiacus BchU<br />
Cfx. aggregans BchU<br />
Chl. vibrio<strong>for</strong>me BchU<br />
Pld. luteolum BchU<br />
Chl. phaeobacteroides BS1 BchU<br />
Ptc. aestuarii BchU<br />
Chl. chlorochromatii BchU<br />
Pld. phaeoclathrati<strong>for</strong>me BchU<br />
Chl. limicola BchU<br />
Chl. phaeobacteroides BchU<br />
Chl. tepidum BchU<br />
Rps. sphaeroides CrtF<br />
Rps. palustris CrtF<br />
Rvx. gelatinosus CrtF<br />
A. thaliana ComT<br />
43 108<br />
45 108<br />
45 108<br />
52 115<br />
29 92<br />
29 92<br />
29 92<br />
30 93<br />
29 92<br />
29 92<br />
29 92<br />
29 92<br />
63 132<br />
59 128<br />
71 140<br />
52 120<br />
A A Y E L G L F R H L A D Q P Q S V E A L A A A T G S R L N R L V K F T D M L V E M E L L A K D A E G R L T L T P F A R Q F F L P P - - - -<br />
A A K D L G L F D L L A S A P R T L A D L A T L T E T V P P R L E K F L I T M E Q I G L V T R Q G D - A W Q L T P F A D Q F F A D - - - - -<br />
A A K D L G L F D L L A A E P R T L A D L A T L T E T V P P R L E K F L I T L E Q V G L V A R D A D - R W K L T P F A E Q F F A D - - - - -<br />
A A L E L D L F T H M A G S P K E A E T L A D D C Q A I P S R F V M L L E A L R Q I G I T L E S D G - K W S L T P L A R A M F V P - - - - -<br />
A A L E L D L F A L L A D G A K D T A T L A R E A G A V P P R L G M L L E A L R Q I G V T T E N A G - Q W D L T P M A R T M F V P - - - - -<br />
A A L E L D L F T E L A E E A K D A E T L S S A L E A V P Q R L E M L L E A L R Q I G I V R I E N G - K W A L T D F A R S M F V P - - - - -<br />
A A L E L D L F K H L A G E A K D V E S L A T S I E A V P Q R L A M L L E A L R Q I G I T R Q E D G - K W S L T P F A S N M F V P - - - - -<br />
A S L E L D L F T H L A G E A K D T E T I A A N V G A I P Q R L V I L L E T L A Q I G V L A K N D G - K W S L T P F A A T M F L P - - - - -<br />
A A L E L D L F T L L A D E A K N V E T L A A S A G A I P Q R L I M L L E A L R Q I G V T A E T G G - K W S L T P F A Q K M F L P - - - - -<br />
A A L E L D L F S Q L A D E A K D T G T L A A A T G S V P P R L G M L L E A L R Q M G V T N E D A G - K W S L T P S A K A M F V P - - - - -<br />
A A V E L D L F S L L A D E P K D T E D L A S S V G A V P M R L G M L L E A L R Q I G I T E E R D G - K W A L T S F S K A M F V P - - - - -<br />
A A I E L D L F S H M A E G P K D L A T L A A D T G S V P P R L E M L L E T L R Q M R V I N L E D G - K W S L T E F A D Y M F S P - - - - -<br />
A L V E L R V L H L V A E G P Q T V Q A L A A A T G L A P E R M Q F L L Q G G A A L K L L K P R R Q R Q F H L G G P G A A F L A V P G V Q A<br />
A C V Q L R L F D I V A E R P A T A E D L A A Q L S L P V D S L R V L L D G A V A L R L L Q M R S G H R Y G L G Q L G A E L R G N P G V L A<br />
A C V R L G V F K M L A D G P L P L A T V A E R C G L S L E A A R R L V A A A D S L E L L D L Q S G D L V T L G R L G A P L V A N E A I T A<br />
S Y L S P V D L A S M A - A P K N P H A P M M I D R L L R F L V A Y S V C T C K L V K D E E G R E S R A Y G L G K V G K K L I K D E D G F S<br />
Cab. thermophilum BchU<br />
Cfx. aurantiacus BchU<br />
Cfx. aggregans BchU<br />
Chl. vibrio<strong>for</strong>me BchU<br />
Pld. luteolum BchU<br />
Chl. phaeobacteroides BS1 BchU<br />
Ptc. aestuarii BchU<br />
Chl. chlorochromatii BchU<br />
Pld. phaeoclathrati<strong>for</strong>me BchU<br />
Chl. limicola BchU<br />
Chl. phaeobacteroides BchU<br />
Chl. tepidum BchU<br />
Rps. sphaeroides CrtF<br />
Rps. palustris CrtF<br />
Rvx. gelatinosus CrtF<br />
A. thaliana ComT<br />
109 176<br />
109 176<br />
109 176<br />
116 183<br />
93 160<br />
93 160<br />
93 160<br />
94 161<br />
93 160<br />
93 160<br />
93 160<br />
93 160<br />
133 195<br />
129 193<br />
141 209<br />
121 188<br />
N P N N E N L T M E P F V R Y A I T I M E R Y H L H L A K V I R G E M D F T A L T P W P P - - R T R E D S A F Y E E I H R S N N H F V R N L<br />
P E R H R N M T M V P F V D Y I A D L I E N Y Y L R L A D V V R G K V D F T S I V P H P P - - R T R E D S L F Y E T L H R S N I Q L F T E L<br />
P D R H R N M T M V P F V D Y I S D L I E N Y Y L R L A D V V R G K V D F T S I V P H P P - - R T R E D S L F Y E T L H R S N I Q L F V E L<br />
Q A D Q P N M Y M V P V G K A M A H L S E N Y Y L K I A D A V R G K H E F R A E V P Y P P - - I S R E D N L Y F E E I H R S N A Y F A I K L<br />
A A D Q P N M F M V P V A K A M A H L A D S F Y L K M A D A V K G N L N F K G E V P Y P P - - V T R E D N W Y F E E I H R S N A H F A I K L<br />
S D E H P N L Y M T P V A K A M A Y T A E N F Y M H M A E A V R G K L D Y K G E T S Y P P - - K T R E E N L Y F E D I H R R N A H F S I K L<br />
N D E H P N L Y M S P V A E A M A Y T A E N F Y M H M A Q A V K G K L D Y K G E T S Y P P - - K T K E E N L Y F E E I H R R N A H F S I K L<br />
N N E L P N L Y M M P V T K A M A H L S E N F Y L K I A D A V R G N H I F K A E V P Y P P - - M T R E D N W Y F E E I H R S N A H F S I L L<br />
N H E N Q N L Y M I P V A K A M A N L S E N F Y L Q I A D A V R G N M N F K G E V A Y P P - - V T R E D N L Y F E E I H R S N A Y F A I M L<br />
D A E H P N L Y M I P V A K A M A H L S D N F Y L K L A D A V K G Q L N F K G E V P Y P P - - V T R E D N W Y F E E I H R S N A H F A I K L<br />
N S E H P N M Y M T P V A R A M V H L S D N F Y M D L A S A V K G T M N F K G E V P Y P P - - V T R E D N W Y F E E I H R S N A H F A I K L<br />
T P K E P N L H Q T P V A K A M A F L A D D F Y M G L S Q A V R G Q K N F K G Q V P Y P P - - V T R E D N L Y F E E I H R S N A K F A I Q L<br />
M W G H H H V L Y R D L A D P V A F L K G E T E P E L A R F W P Y V F G A G G A T D P - - - - - - - E V T A K Y S R L M T E S Q G L V A E D<br />
L V A H H A M L Y R D L A D P V A L L R A P R G G G E L A A Y W A Y V R G E R P G E - - - - - L P A D A I A S Y T A L M A A S Q P M I A R E<br />
M V E H H A T L Y G D L T D P V A L L R G - Q G R P A M A G Y W P Y A A A A E A G P G Q P A T L A S E K V A E Y S K L M T A S Q P L V A R E<br />
I A P Y V L A G C T K A K G G V W S Y L T E A I Q E G G A S A W E R A N E A L I F E Y M K - - K N E N L K K I F N E S M T N H T S I V M K K<br />
Cab. thermophilum BchU<br />
Cfx. aurantiacus BchU<br />
Cfx. aggregans BchU<br />
Chl. vibrio<strong>for</strong>me BchU<br />
Pld. luteolum BchU<br />
Chl. phaeobacteroides BS1 BchU<br />
Ptc. aestuarii BchU<br />
Chl. chlorochromatii BchU<br />
Pld. phaeoclathrati<strong>for</strong>me BchU<br />
Chl. limicola BchU<br />
Chl. phaeobacteroides BchU<br />
Chl. tepidum BchU<br />
Rps. sphaeroides CrtF<br />
Rps. palustris CrtF<br />
Rvx. gelatinosus CrtF<br />
A. thaliana ComT<br />
177 245<br />
177 244<br />
177 244<br />
184 251<br />
161 228<br />
161 228<br />
161 228<br />
162 229<br />
161 228<br />
161 228<br />
161 228<br />
161 228<br />
196 263<br />
194 261<br />
210 277<br />
189 250<br />
L V E E A D - L E G V T H L L D V G G G N G D I A I A L A K R F P S I E R I T L I N L P S A L D I V R D N V A A H G L S D R I V P V A L D M<br />
L I K R A R - L A D V Q T L I D V G G G I G D I A A A L C Q A F P Q L K - V T L I N L P S A L D L V R E N V A A K G L A D R I T P V A I D M<br />
L V K R A R - L A D V Q T L V D V G G G I G D I A A A L C Q A F P N L K - V T L I N L P S A L D L V R E N V A A K G L S D R I T P V A I D M<br />
L L E A A E - L S G T K N L V D V G G G I G D I S A A L L K K Y P E L N - S T I L N L P G A I G L V D E N A A A Q G V G G R L K G A A V D I<br />
L L E E A R - L E G T K T L V D V G G G I G D I S A A L L K K H P Q L D - S T I L N L P G A I G L V D E N A V A Q G V G G R L R G A A V D I<br />
L L E E A D - L Q N N K K M V D V G G G I G D I S A A L C N K F S Q L D - T T I L N L P G A I E L V N E N A A E K G L A D R L R G A A V D I<br />
L L E E A R - L D D T K K M I D V G G G I G D I A A A L C K H Y P N L D - T T I L N L P G A I E L V D E N A A E K G L G D R L R G A A V D I<br />
L L E E A N - L S N V K T L V D V G G G I G D I S A A L L Q K Y P Q M D - S T I L N L P G A V E L V N E N A T E K G L A D R L R G S V V D I<br />
L L E E A D - L E T A K K L V D V G G G I G D I S A A L L K K F P Q L H - S T I L N L P G A V E L V N E N A V E K G V D N R L K G A A V D I<br />
L L E E A D - L S A A K T M V D V G G G I G D I S A A L L Q K F T N L Q - S T I L N L P G A I E L V N E N A I E K G V G D R L K G V A V D I<br />
L L E E A D - L S A A K T L V D V G G G I G D I S A A L L K K Y T G L Q - S T I L N L P G A V P L V N D N A A E K G V A D R L R G A V V D I<br />
L L E E A K - L D G V K K M I D V G G G I G D I S A A M L K H F P E L D - S T I L N L P G A I D L V N E N A A E K G V A D R M R G I A V D I<br />
A L R L V D - L M G V R R L M D V G G G T G A F L A A V G R A Y P L M E - L M L F D L P V V A E A A P Q R L T E A G L A G R F T V H G G S F<br />
V L H V Y S - F R D H R C L L D V G G G D G S F L S A V A A Q A P Q L R - C M L F D L P A V A E K A A A R F E A N G L K G R A T A V G G S F<br />
V I A A Y R - F G R H K V L M D V G G G E G A F V R C V A A E V P G V E - L R V F D L P A V A E R A N E N F Q R W G L A G R A Q A F G G D F<br />
I L E N Y I G F E G V S D F V D V G G S L G S N L A Q I L S K Y P H I K - G I N F D L P H I V K E A P Q I H G V E H I G G - - - - - - - D M<br />
Cab. thermophilum BchU<br />
Cfx. aurantiacus BchU<br />
Cfx. aggregans BchU<br />
Chl. vibrio<strong>for</strong>me BchU<br />
Pld. luteolum BchU<br />
Chl. phaeobacteroides BS1 BchU<br />
Ptc. aestuarii BchU<br />
Chl. chlorochromatii BchU<br />
Pld. phaeoclathrati<strong>for</strong>me BchU<br />
Chl. limicola BchU<br />
Chl. phaeobacteroides BchU<br />
Chl. tepidum BchU<br />
Rps. sphaeroides CrtF<br />
Rps. palustris CrtF<br />
Rvx. gelatinosus CrtF<br />
A. thaliana ComT<br />
246 314<br />
245 312<br />
245 312<br />
252 319<br />
229 296<br />
229 296<br />
229 296<br />
230 297<br />
229 296<br />
229 296<br />
229 296<br />
229 296<br />
264 333<br />
262 331<br />
278 347<br />
251 318<br />
Y R D P Y P A - C D A V L F A R I L Y P F N A Q L C A M L L G K A H A A L P P G G R V V V A D M L L R D E R G L A N Y D Y L A H F L T S I G<br />
Y R E P Y P P - G D A V L F S R I L Y P M N A Q F C T M L L R K A Y D A L P S G G R I L I L D M V I S D P N - H P N Y D Y L T H Y L C A I G<br />
Y R D P Y P P - G D A V L F S R I L Y P M N A Q F C S M L L K K A Y D A L P S G G R V L I L D M V I S D P Q - H P N Y D Y L T H Y L C A I G<br />
Y K E A Y P S - A D A V M F C R I L Y S A N E Q L T G M M C R K A Y D A L P A G G R I L I L D M I V D N P E - E P N F D Y L S H Y I L G A G<br />
Y K D A Y P A - A D A V M F C R I L Y S A N E Q L T E M M C R K A Y D A V P A G G K V L I L D M I V D D R E - N P N F D Y L S H Y I L G A G<br />
Y R D E Y P A - A D A V M F C R I L Y T A N E Q L T E M M C T K A F N A L E A G G K L L V L D M I V D E Q D - N P N Y D Y L S H Y I M G I G<br />
Y R D E Y P A - T D A V M F C R I L Y S A N E Q L T E M M C T K A F N A L S P G G K V M V L D M I V D D P S - N P N Y D Y L S H Y I M G I G<br />
Y K E E Y P K - A D A V M F C R I L Y S A N E Q I T S M M C K K A L D A L Q E G G K V L I L D M I I D E P E - D P N F D Y L S H Y I L G A G<br />
Y K D A Y P E - A D A I M F C R I L Y S A N E Q L T T M L C K K A Y D A L T P G G K V L I L D M I I D D P E - N P N F D Y L S H Y I L G A G<br />
Y K D V Y P K - A D A V M F C R I L Y S A N E Q L S E M M C R K A F E A L P S G G K V L I L D M I I D D P E - S P N F D Y L S H Y I L G A G<br />
Y K E A Y P Q - A D A V M F C R I L Y S A N E Q L T D M M C R K A F E A L S A G G K L I I L D M V I D D P E - T P N F D Y L S H Y I L G A G<br />
Y K E S Y P E - A D A V L F C R I L Y S A N E Q L S T I M C K K A F D A M R S G G R L L I L D M V I D D P E - N P N F D Y L S H Y I L G A G<br />
R D D P L P L G A D A I S L V R V L F D H S D E T V K L L L H R V R E A L P A G G R V I V A E A M S G G A R P H R E T D T Y M A F Y T A A M<br />
T T D P L P E G A D I V S L V R V I H D H D D E V V E A L L R A V H K A L P D N G K L L I A E P I A G L P G T A A I S D A Y F A F Y L Y A M<br />
F A D E L P R G A D V I S L V R V A F D H P D E R V L T L F K N V R R A L P D N G T L V L A E A M A E V P G V E P I G D A Y F G F Y L L A M<br />
F - D E I P R - G E V I L M K W I L H D W N D E K C V E I L K N C K K A L P E T G R I I V I E M I V P R E V S E T D L A T K N S L S A D L T<br />
Cab. thermophilum BchU<br />
Cfx. aurantiacus BchU<br />
Cfx. aggregans BchU<br />
Chl. vibrio<strong>for</strong>me BchU<br />
Pld. luteolum BchU<br />
Chl. phaeobacteroides BS1 BchU<br />
Ptc. aestuarii BchU<br />
Chl. chlorochromatii BchU<br />
Pld. phaeoclathrati<strong>for</strong>me BchU<br />
Chl. limicola BchU<br />
Chl. phaeobacteroides BchU<br />
Chl. tepidum BchU<br />
Rps. sphaeroides CrtF<br />
Rps. palustris CrtF<br />
Rvx. gelatinosus CrtF<br />
A. thaliana ComT<br />
315 356<br />
313 354<br />
313 354<br />
320 362<br />
297 339<br />
297 339<br />
297 339<br />
298 338<br />
297 339<br />
297 339<br />
297 339<br />
297 338<br />
334 379<br />
332 373<br />
348 390<br />
319 363<br />
V D P A I M T F K R Q - - D E Y F D V F K Q L G F I D A R K V E R H G H V L Y Q A R K A<br />
M G F S V L E F K D H - - A V Y P D L L R Q V G F T D V T L D E G Y E H V L Y Q A V K P<br />
M S F S V L E F K D H - - A I Y P D L L R Q I G F T D V T F D E G Y D H V L Y Q A V K P<br />
L P F S V L G F K S Q - - E S Y R G I L E E I G F S D I S I T R R Y N H L L C Q A V K K G<br />
M P F S V L G F K S Q - - E A Y R Q I L E S I G F T D V R M V R K Y D H L L C E A V K P A<br />
M P F S V L G F K E Q - - S N Y K N I L E K I G F S D V R M V R R Y D H L Y V E A V K P S<br />
M P F S V L G Y K D Q - - D N Y K H I L E K I G F T D V R M V R R Y D H L F V E A V K P A<br />
M P F S V L G F K Q Q - - E R Y K E L L E A I G F R D V R I V R K Y G H L L C E A V K<br />
M P F S V L G F K Q Q - - S A Y K E I L E S L G F R D I R T V R K Y G H L L C E A V K A L<br />
M P F S V L G F K P Q - - N A Y K E I L E K I G F T G V R I V R R Y D H L L C E A V K P A<br />
M P F S V L G F K P Q - - S A Y K E I L E A I G F T D V R M V R K Y D Q L L C E A V K P A<br />
M P F S V L G F K E Q - - A R Y K E I L E S L G Y K D V T M V R K Y D H L L V Q A V K P<br />
R T G R V R S A A E I - - A E L L T G Q G F S E I K I F P G L R P Y V A S A V T A V R P S D A P<br />
G S G K A R S F P R - - - - - L Q A M L E N A G F S Q V T L H P V P M P L V A S V I T A S K R<br />
G R G R P R S A A R - - - - - L T Q M L H E A G F A S V R P L S T H M P L Q A G V L V A R C T A<br />
M M S L T S G G K E R T K K E F E D L A K E A G F K L P K I I Y G A Y S Y W I I E L Y P N<br />
Figure 11S
Figure 12S<br />
AcsF Alignments<br />
Cab. thermophilum AcsF<br />
Cfx. aggregans AcsF<br />
Cfx. aurantiacus AcsF<br />
Rfx. sp. RS1<br />
Rfx. castenholzii AcsF<br />
Rps. palustris AcsF<br />
Rb. sphaeroides AcsF<br />
Rb. sphaeroides 2.4.1 AcsF<br />
Synechocystis sp. 6803 AcsF<br />
G. violaceus AcsF<br />
Nostoc sp. 7120 AcsF<br />
Por. yezoensis AcsF<br />
A. thaliana AcsF<br />
10 20 30 40 50 60 70 80 90 100 110<br />
M I A T E S V I R E T K A A A A D K S L I S P R F Y V T D Y K K I N A L R F D H - - - - - M A E A F K A M H K R F A D D P N K H F<br />
M F W I N P L M M E M P G E F S R T T R H A L K E A I L S P R F Y T T D F K A I D K L E I D R N G - - - L R D E F D W I R D E F A Y D Y N R T H<br />
M F W I N P L M M E M P G E F S R T T R H A L K E A I L S P R F Y T T D F K A I D R L E I D R N G - - - L R D E F D W I R D E F A Y D Y N R T H<br />
M M N I P G E Y T P T T R H A L R E A I L N P R F Y T T D F R A I D R L N V A H - - - - - M R D E F D W I R N E F E V D Y N R K H<br />
M I N M P G E Y S P T T R H A L R E A I L N P R F Y T T D F R A I D R L N V A H - - - - - M R D E F D W I R N E F E F D Y N K K H<br />
M I P M E G G A Q G A L R T N S R P A I K G S V D S L N I A K E D T I L T P R F Y T T D Y A A M D K L D V S L - - - - - V R S E W N A M M N E M R A D Y N K A H<br />
M N A S P G D L S P A L D P E A V A D T T A M A T E T H I L N P R F Y T T D F D E L D R V D V T P - - - - - I R A Q W D E L I A E M K A D P N K G H<br />
M N A P A G G L S P A L T P E A V A D T T A M A T E T H I L N P R F Y T T D F D E L D R V D V T P - - - - - V R K D W D A L I A E M K A D P N K A H<br />
M V N T L E K P G F D E I R P G V K T P A K E T I L T P R F Y T T D F D E M A K M D I S P N - - - - - E D E L R A I L E E F R V D Y N R H H<br />
M V D A L R E A N T A S Q S E R R E T L L T P R F Y T T D F E A L A K L D I S A M - - - - - E A D F E A L L A E F Q A D Y N R E H<br />
M V D S L K K P G F E E I R P G V K V P A K E T L L T P R F Y T T D F D E M A R M D I S V N - - - - - E D E L L A I L E E F R T D Y N R H H<br />
M Q T T I N N G Q T S S K E T L L T P R F Y T T D F E E M A N M D I S G N - - - - - Q E D F L A I L E E F R A D Y N S E H<br />
M A A E M A L V K P I S K F S S P K L S N P S K F L S G R R F S T V I R M S A S S S P P P P T T A T S K S K K G T K K E I Q E S L L T P R F Y T T D F E E M E Q L F N T E I N K N L N E A E F E A L L Q E F K T D Y N Q T H<br />
M A A E M A L V K P I S K F S S P K L S N P S K F L S G R R M . E T T . A . . E . I L . P R F Y T T D F . . D . L D . . N G K N L R . E F . A I . E F . . D Y N . H<br />
Cab. thermophilum AcsF<br />
Cfx. aggregans AcsF<br />
Cfx. aurantiacus AcsF<br />
Rfx. sp. RS1<br />
Rfx. castenholzii AcsF<br />
Rps. palustris AcsF<br />
Rb. sphaeroides AcsF<br />
Rb. sphaeroides 2.4.1 AcsF<br />
Synechocystis sp. 6803 AcsF<br />
G. violaceus AcsF<br />
Nostoc sp. 7120 AcsF<br />
Por. yezoensis AcsF<br />
A. thaliana AcsF<br />
120 130 140 150 160 170 180 190 200 210 220<br />
F R R D D - - - D P D F D Q D F S H L P P S F K D F L I R G C I G E F S G A L L Y K E I A D R I E D - - P I I S D T Y R L M A R D E G R H S G F I R L A M R D I N T D L D L Q M L Q K E K E F V S M H P K I I L Y T T Y L S<br />
F R R T D E F L Q N F D D M P E - - - R D A F I D F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L R A I F K Y M S R D E G R H A G F L N R T M A D L N V A L D L T V L Q K R K K Y T Y F Q P K F I F Y S V Y L S<br />
F R R T D E F L Q D F D D M P E - - - R D S F I D F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L R A I F K Y M S R D E G R H A G F L N R T M A D L N V A L D L T V L Q K R K K Y T Y F Q P K F I F Y S V Y L S<br />
F V R N D E F L A N F D D M P A - - - R D L F I E F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L K A I F R C M S R D E G R H A G F L N K T M A D L G V E M N L Q V L H T R K K Y T Y F Q P K F I F Y S V Y L S<br />
F V R N E E F L A N F D D M P A - - - R D L F I E F L E R S C T A E F S G C L L Y A E M V K H L H - - D P T L R A I F R C M S R D E G R H A G F L N K T M A D L G V E M N L Q V L H T R K K Y T Y F Q P K F I F Y S V Y L S<br />
F K K T D E F L E S D L D K L P P E L L I E F K D F L V S S L T A E F S G C V L Y S E I K K R I K - - N P E I R E L F G L L S R D E A R H A G F I N E I L K D H G I G V D L S F L T K V K K Y T Y F R P K F I F Y A T Y L S<br />
F K K T D A W D R I D W E A M D P A L R V E F I D F L V S S C T A E F S G C V L Y K E M K R R G S - - N P D I C E L F S Y M A R D E A R H A G F I N D A L R E A G V A V N L G F L T K A K K Y T Y F R P K F I Y Y A T Y L S<br />
F K K T E A W D R I D W D G M D P A L R V E F I D F L V S S C T A E F S G C V L Y K E M K R R G S - - N P D I R E L F N Y M A R D E A R H A G F I N D A L R E A G V A V N L G F L T K A K K Y T Y F R P K F I Y Y A T Y L S<br />
F V R N E S F N K S W D H I D G E - K R Q L F V E F L E R S C T A E F S G F L L Y K E L G R R L K N K N P L L A E C F N L M S R D E A R H A G F L N K A M S D F N L S L D L G F L T K S R K Y T F F K P K F I F Y A T Y L S<br />
F Q R D A G F V E Q A H Q I D P Q - W R A L M L E F L E R S C T S E F S G F L L Y K E I S R R L K A A N P L L A E C F K A M S R D E A R H A G F L N K A M G D Y D L G L D L S N L A K Q K K Y T F F K P K F I F Y A T Y L S<br />
F V R D A E F E K S W D H I D G D - T R R L F V E F L E R S C T A E F S G F L L Y K E L G R R L K D K S P V L A E C F N L M S R D E A R H A G F L N K A M S D F N L S L D L G F L T K S R N Y T F F K P K F I F Y A T Y L S<br />
F I R D E E F N Q S W S N L E H K - T K S L F I E F L E R S C T A E F S G F L L Y K E L S R K L K D R N P V I A E C F L L M S R D E A R H A G F L N K A I G D F N L S L D L G F L T K S R K Y T F F S P K F I F Y A T Y L S<br />
F V R N K E F K E A A D K L Q G P - L R Q I F V E F L E R S C T A E F S G F L L Y K E L G R R L K K T N P V V A E I F S L M S R D E A R H A G F L N K G L S D F N L A L D L G F L T K A R K Y T F F K P K F I F Y A T Y L S<br />
F R . E F . D D . L R . F I E F L E R S C T A E F S G C L L Y K E . . R R L . N P L E . F . M S R D E A R H A G F L N K A M D . N . . L D L F L T K K K Y T Y F . P K F I F Y A T Y L S<br />
Cab. thermophilum AcsF<br />
Cfx. aggregans AcsF<br />
Cfx. aurantiacus AcsF<br />
Rfx. sp. RS1<br />
Rfx. castenholzii AcsF<br />
Rps. palustris AcsF<br />
Rb. sphaeroides AcsF<br />
Rb. sphaeroides 2.4.1 AcsF<br />
Synechocystis sp. 6803 AcsF<br />
G. violaceus AcsF<br />
Nostoc sp. 7120 AcsF<br />
Por. yezoensis AcsF<br />
A. thaliana AcsF<br />
230 240 250 260 270 280 290 300 310 320 330<br />
E V I G Y F R Y I N I Y S H L E A H P E Y R Y H P I F K Y F G G W C Q D E L K H G D F I G L Q L E A Q K H L Y L S G G I N R L L I R F F S L A V Y V T M W L R D L K A Q E F Y A S I G L N W R E Y D L K V I R E T N A Y A Q<br />
E K I G Y S R Y I N I Y R H L E Q N P Q Y C I H P I F K W F E Q W C N D E Y R H G E F F A L L M R S Q P E M L E G - - A N R R W I R F F L L A V Y A T M Y L N D A R R A N F Y A A L G L N W R D Y D Q K V I R L C N D I A T<br />
E K I G Y S R Y I T I Y R H L E R N P Q Y C I H P I F K W F E Q W C N D E Y R H G E F F A L L M R S Q P E M L E G - - V N R R W I R F F L L A V Y A T M Y L N D A R R S N F Y A A L G L D W R D Y D Q T V I R L C N D I A T<br />
E K I G Y A R Y I T I Y R H L Q K Y P Q G M I H P I F K W F E K W C N D E Y R H G E F F S L L M R S Q P D L L R G - - G N L R W I R F F L L A V Y A T M Y L N D A R R A G F Y Q A L G L D W R D Y D Q R V I R L T N H I A T<br />
E K I G Y A R Y I T I Y R H L Q H H P Q G M I H P I F K W F E K W C N D E Y R H G E F F S L L M R S Q P D L L R G - - G N L R W I R F F L L A V Y A T M Y L N D A R R A G F Y E A L G L N W R D Y D Q R V I R L T N H I A T<br />
E K I G Y A R Y I T I Y R Q M E R H P E R R F H P I F K W F E R W C N D E F R H G E A F A L L M R A D P S L L R G - - V N K L W I R F F L L A V F A T M Y V R D H M R P A F Y D A L G V D A T D Y D M Q V F R V T T E I S K<br />
E K I G Y A R Y I T I Y R H L E A N P E H R F H P I F K W F K E W C N D E F R H G E A F A L L M K T D P K L T D S T - V N R L W I R F F L T A V Y S T M W V R D H A R P E F H K A L G V D I D W Y D Q E V F R K T S E I A R<br />
E K I G Y A R Y I T I Y R H L E A N P E H R F H P I F K W F K E W C N D E F R H G E A F A L L M K T D P K L T D T T - V N R L W I R F F L T A V Y S T M W V R D H A R P E F H K A L G V D I D W Y D Q E V Y R K T S E I A R<br />
E K I G Y W R Y I T I Y R H L E K N P N D C I Y P I F E F F E N W C Q D E N R H G D F F D A I M R A Q P H T L N D W - K A K L W C R F F L L S V F A T M Y L N D T Q R A D F Y A C L G L E A R S Y D K E V I E K T N E T A G<br />
E K I G Y W R Y I A I F R H L E R H P E N R I H P L F N Y F K N W C Q D E N R H G D F F G L I L K S Q P Q L L K G P - I N R L W V R F F L L S F C L T M Y L N D R K P H N L Y S A L G L D A S H Y A M E V L R Q T N L D S G<br />
E K I G Y W R Y I T I Y R H L E A H P E D R I Y P I F R F F E N W C Q D E N R H G D F F D A I M K S Q P Q M L N D W - K A K L W S R F F L L S V F A T M Y L N D I Q R K D F Y A T I G L D A R D Y D I Y V I K K T N E T A G<br />
E K I G Y W R Y I T I Y R H L E Q H P E H R I Y P I F R F F E N W C Q D E N R H G D F F A A L L K S Q P H F L N D W - K A K M W C R F F L L S V F A T M Y L N D F Q R I D F Y N A I G L D S R Q Y D M Q V I R K T N E S A A<br />
E K I G Y W R Y I T I Y R H L K E N P E F Q C Y P I F K Y F E N W C Q D E N R H G D F F S A L M K A Q P Q F L N D W - Q A K L W S R F F C L S V Y V T M Y L N D C Q R T N F Y E G I G L N T K E F D M H V I I E T N R T T A<br />
E K I G Y R Y I T I Y R H L E P E R I H P I F K W F E . W C N D E . R H G E F F . L L M . S Q P L L G . N . L W I R F F L L A V Y A T M Y L N D R F Y A L G L D R . Y D V I R T N . I A<br />
Cab. thermophilum AcsF<br />
Cfx. aggregans AcsF<br />
Cfx. aurantiacus AcsF<br />
Rfx. sp. RS1<br />
Rfx. castenholzii AcsF<br />
Rps. palustris AcsF<br />
Rb. sphaeroides AcsF<br />
Rb. sphaeroides 2.4.1 AcsF<br />
Synechocystis sp. 6803 AcsF<br />
G. violaceus AcsF<br />
Nostoc sp. 7120 AcsF<br />
Por. yezoensis AcsF<br />
A. thaliana AcsF<br />
340 350 360 370 380 390 400 410 420 430 440<br />
R V F G L R L D V D N P K F I R I L D H M A A C Y A K M L K R E A D G A S K L A L M P L Q V A M A V D Y L R L L A M K V R T D A P P P I P E Y P V A P V I K G F A T K Y K G Q Y K G Q P A M A *<br />
Q V Y P E T M N L D D P R F F G Y M D K L V E Y D R K L R E L E G R K D P V A L A Q S T R L K A A I A L R L L A I Y R L P T R K T T E A I R W K G L P G F P N Y P G P N R P Q R V A A A D<br />
Q V Y P E T M N L D D P R F F A Y M D K L V E Y D R A L R A L E S R K D P I A M A H S T R L K A A I A L R L L A I Y R L P T R K T T E A I R W K G L P G F P N Y P G P N R P Q R V V A A D<br />
Q V F P V T L P V D D P R F F R H L D A C V T Y D A Q I R A L E G R S D P I A T A Q R A R L G A A I A A R L L A T Y R L P V V P T T D A N R W Q G L E G F P N Y P G P G W K Q D A T L N Q R V I S A<br />
Q V F P V T L P V D D P R F F R H L D A C V R Y D A Q I R A L E G R N D P I A Q V Q R A R L G A G I A A R L L A T Y R L P P A P T T D A N R W K G L E G F P N Y P G P G W K Q D A S L N E R V I S A<br />
Q V F P V M I N L D D P R F L A G L E R L R K V S E K I A E - S R S Q G F V G K L K R P L Y A A S A A I A F G R L F L L P A K R N E L P R V I G L R P A W<br />
Q I F P V E L D I D H P R W K P A L N R M N A A F L R M D R G N R R G G L A G R L D K V L G G A Q A L A A F T A L Y T I P V R T N A L P A N V R L E P S Y<br />
Q I F P V E L D I D H P R W K P A L R R M N E A F L R I D R G T R R G G I A G R L E K A L G G A Q A L A A F V S L Y T I P V R T H T L P E N V R L E P S Y<br />
R V F P I I L D V N N P E F Y N R L E T C V S N N E Q L R A I D A S G A P G V I K A L R K L P I F A S N G W Q F I K L Y L M K P I A V D Q L A G A V R<br />
K V F P L I L D L D N P R F Q R H L D R C A D L N L Q L E A L S G K S P - - - - - - L A R L P V I A A L A G T I A Q L F L L K P V A P P A P E M V R<br />
R V F P I I L D V D S P E F Y E R L D I C V E N S E K L S A I A S S N T P K F L Q F F Q K L P V L A S T G W Q L L R L Y L M K P I D A V S A Q G A A R<br />
R V F P V A L D V D N P K F F K Y L D T C A C D N R A L I D I D N N N S P L F I K S I V K I P L Y F S L F A N L L K I Y L I K P I D S K T V W N T V R<br />
R I F P A V L D V E N P E F K R K L D R M V V S Y E K L L A I G E T D D A S F I K T L K R I P L V T S L A S E I L A A Y L M P P V E S G S V D F A E F E P N L V Y<br />
Q V F P . L D V D P R F . L D . . V . L A . . . P . . . . . L A . . . . . . . . . . P . P . . . . P N Y P G P Q R V I S A
Figure 13S<br />
absorbance at 667 nm<br />
absorbance<br />
14<br />
12<br />
10<br />
8<br />
6<br />
4<br />
2<br />
0<br />
-2<br />
Absorbance spectrum<br />
of peak 3b<br />
436<br />
400 600 800<br />
Bchl c F<br />
wavelength (nm)<br />
667<br />
Bchl c GG<br />
1 2 3 4 1 2 3 4<br />
15 20 25 30 35 40 45 50<br />
elution time (min)<br />
1a<br />
1b<br />
2b<br />
2a<br />
3a<br />
3b<br />
4b<br />
Chl a
Table 1S. Oligonucleotide primers used in this study.<br />
Gene Description Forward (5’ to 3’) Reverse (5’ to 3’)<br />
Cab. thermophilum 16S rRNA Ribosomal RNA used<br />
as phylogenetic<br />
marker<br />
Anoxybacillus sp. 16S rRNA Ribosomal RNA used<br />
as phylogenetic<br />
marker<br />
Bacterial 16S rRNA Ribosomal RNA used<br />
as phylogenetic<br />
marker<br />
Cab. thermophilum recA DNA repair protein<br />
used as phylogenetic<br />
marker<br />
Cab. thermophilum pscA Type 1 reaction center<br />
protein<br />
Cab. thermophilum fmoA BChl a-binding<br />
protein<br />
Cab. thermophilum csmA BChl a-binding<br />
protein located in the<br />
baseplate of<br />
chlorosomes<br />
Cab. thermophilum bchU C-20<br />
methyltransferase <strong>for</strong><br />
BChl c biosynthesis<br />
Cab. thermophilum acsF Oxidative cyclase <strong>for</strong><br />
BChl a,c biosynthesis<br />
under oxic conditions<br />
GAT CCT GGC TCA<br />
GAA TC (38)<br />
CGA AAG TCT GAC<br />
GGA GCA AC<br />
AGA GTT TGA TCA<br />
TGG CTC AG (46)<br />
GCA TGG AAG GCG<br />
CTG TAG CC<br />
GCT TTT CGT CCT<br />
ATG CCA ACG<br />
GGC AAG ATT ACG<br />
GTC GGA AAG<br />
GTC GTG GAC TCC<br />
ATC GTC<br />
TTC TCT ACG AGC<br />
AGT ATT TGC GTG<br />
CAG GAT TTC TCC<br />
CAT CTC CCA C<br />
GGC TTA CAC AGG<br />
ATA CC<br />
TCA TCG TTT ACG<br />
GCG TGG AC<br />
GGT TAC CTT GTT<br />
ACG ACT T (46)<br />
CAG ACA GCC TGC<br />
TTC CTA CC<br />
AGG GGT CAA ACC<br />
AAC CGT AAG G<br />
CCC TCA ATA GGA<br />
ACA CTA AAC CCC<br />
TCC TTC GGC TGT<br />
GAA ACG<br />
CGG TGG ATT TCC<br />
TCA TAA AAC G<br />
CCA ATA AAG TCG<br />
CCG TGT TTG AT
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