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114 Application of Alternative Food-Preservation Technologies to Enhance Food Safety & Stability, 2010, 114-142 Use of High Pressure Processing for Food Preservation Antonio Bevilacqua, Daniela Campaniello and Milena Sinigaglia* Department of Food Science, Faculty of Agricultural Science, University of Foggia, Italy Antonio Bevilacqua, Maria Rosaria Corbo and Milena Sinigaglia (Eds) All rights reserved - © 2010 Bentham Science Publishers Ltd. CHAPTER 8 Abstract: High pressure processing has been proposed since the beginning of the 1900, as a suitable mean for reducing food contamination by pathogens and spoiling microorganisms. It is defined as non-thermal treatment that uses the pressure (300-700 MPa, in some cases up to 1000 MPa) as the main preservation method. Based on the different ways to achieve pressure increase, we can distinguish between High Hydrostatic Pressure (HHP) and High Pressure Homogenization (HPH); HHP attains pressure rise through a fluid, whereas in HPH treatments pressure increases as a consequence of forcing product through a small valve (homogenizing valve). Both these approaches have been proposed for different kinds of foods (HHP, for chopped onions, apple sauce and apple sauce/fruit blends as eat-on-to-the-go single serve tubes; HPH, for milk and juices) and currently used in many industrial applications. The chapter proposes an exhaustive description of both these methods, including the mode of actions against the microorganisms, the modifications on foodstuffs, a possible combination with some other hurdles and some examples of industrial applications. Finally, in the case of HHP there is a report on its safety and implications on health, based on some publications of Public Agencies. Key-Concepts: High Hydrostatic Pressure, Homogenization, Effects of pressure on microorganisms, Equipments. HIGH HYDROSTATIC PRESSURE HIGH HYDROSTATIC PRESSURE: AN INTRODUCTION There are several definitions of the term high pressure processing; hereby, we will use the most simple, i.e. High Pressure Processing (HPP) is a non-thermal food processing, that uses the pressure (300-700 MPa, in some cases up to 1000 MPa) as the main preservation method [1]. Due to the fact that pressure increase is achieved through a fluid (for example water), this process has been also referred to as High Hydrostatic Pressure (HHP) as opposite to the High Pressure of Homogenization (HPH), where the increase of the pressure is obtained forcing the product through a small valve (homogenizing valve). Bert Hite was the first to use this method as an alternative approach for food preservation; he pressurized many kinds of foods and beverages in the late 1890s and at the beginning of the 20 th century [2]. Since these initial efforts, other researchers tried to use this approach, but only in the 1980s the suitability of HHP as a food preservation method was realized [2]. The first HHP-treated products (jams and jellies) appeared in 1991 in Japan; in 2001, guacamole (pressuretreated avocado) entered US marketplace, followed by HHP salsa and in 2004 by chopped onions, apple sauce and apple sauce/fruit blends as eat-on-to-the-go single serve tubes. In the EU a consumer research [3] reported a 67% of acceptability from consumers of three different European countries (France, Germany and United Kingdom), thus opening the way for the marketing of HHP-treated products. An interesting description of HHP treatment can be found in the paper of Riva [4]: We should imagine two elephants (10-12 tons) laid on a penny; the coin is subjected to a pressure of ca. 900 MPa. Now, we should image that the same pressure has been applied on a egg through water: you don’t break the egg, but you cook it *Address correspondence to this author Milena Sinigaglia at: Department of Food Science, Faculty of Agricultural Science, University of Foggia, Italy; E-mail: m.sinigaglia@unifg.it

High Pressure Processing Application of Alternative Food-Preservation Technologies 115 without increasing the temperature, thus obtaining a “safe” and “fresh” egg, without off-odours. This is the HHP processing. This simple imagine offers a friendly description of how HHP works: hydrostatic pressure is usually applied to food products through a water bath that surrounds the product; it can be applied both to liquid and packed solid foods. Riva [4] reported that HHP is based on 5 basic principles: 1. Le Chatelier’s principle: pressure enhances reactions leading to a volume decrease (e.g. starch gelatinization, protein denaturation and phase transitions). Le Chatelier’s principle can explain the antimicrobial effectiveness of HHP, as high pressures denatures proteins, solidifies lipids and destabilizes biomembranes [2]. 2. Adiabatic heating: the increase of pressure results in a uniform increase of the temperature. This phenomenon can be described through the following equation: dT dP T C where T is the temperature (K); P, the pressure (Pa); α, the thermal expansion (1/K); ρ, the density (kg/m 3 ); Cp, the heat capacity (J/kg*K). This equation indicates that temperature increase depends on the characteristics of the system and the initial temperature: for example it has been evaluated that water temperature increases of 2.8-4.4°C/100 MPa (2.8, 3.8 and 4.4 at 20, 60 and 80°C respectively); otherwise the temperature of oil increases of 6-8°C/100 MPa. 3. Isostatic rule: HHP processing is not affected neither by the volume nor by the shape of the foods; the pression is uniformly distributed around and throughout the product. 4. Squeezing: pressure enhances ionization phenomena inside the system, thus resulting in little changes of the pH. 5. Energy of compression: energy input required by HHP is lower than that used in the traditional thermal treatments; therefore, at room temperature pressure can affect only hydrogen and ionic bonds. In contrast, covalent bonds remain unchanged. Nowadays, HHP has been proposed and applied for the preservation of different product, as a suitable alternative to the traditional heat processing. In the following paragraphs, the reader will find some details on the antimicrobial effectiveness of HHP, a brief description of the physico-chemical modifications caused by pressure in foods, some examples of the application of this approach for some foods and a safety evaluation of the method. EFFECT OF HHP ON THE MICROORGANISMS OF FOODSTUFFS It is well known that HHP can be used successfully for the inactivation of the pathogens and spoiling microflora of foodstuffs. As regards the kind of resistance, different reports suggest the following hierarchy of resistance: Bacteria (cells)>fungi>protozoa-parasites and amongst the bacteria, the Gram positive are more resistant than Gram negative ones, thus highlighting that pressure resistance could be inversely related to cell dimension, although there are some exceptions to this general statement [2]. The viruses cannot be included in this scale, as they are characterized by a broad range of sensitivity/resistance [2]. Pressure treatments at 400-600 MPa for 5-20 min at various temperatures are able to inactivate the vegetative forms of foodborne pathogens (see Table 1); however, it is important to underline that pressure effectiveness is influenced strongly by the temperature, the kind of treatment (single or multi-step) and food components. A final consideration on the bacteria is the following: amongst Gram positive bacteria, lactic acid bacteria appeared as the most resistant ones. Despite bacteria sensitivity, HHP cannot be used to inactivate spores. In fact, bacterial spores are the most difficult life-forms to eliminate with hydrostatic pressure [2]; for example, Hoover et al. [2] reported that it was possible to detect viable spores of Bacillus spp. after a treatment at 1700 MPa for 45 min at room temperature. This report, along with other data available in the literature [5], suggests that HHP alone cannot be used to inactivate spore-formers; in contrast the use of the hurdle approach (i.e. the combination of two or more preserving elements) is a reliable way [2, 6]. In the case of bacterial spores, it has been suggested the p

114 Application <strong>of</strong> Alternative Food-Preservation Technologies to Enhance Food Safety & Stability, 2010, 114-142<br />

Use <strong>of</strong> High Pressure Processing for Food Preservation<br />

Antonio Bevilacqua, Daniela Campaniello and Milena Sinigaglia*<br />

Department <strong>of</strong> Food <strong>Science</strong>, Faculty <strong>of</strong> Agricultural <strong>Science</strong>, University <strong>of</strong> Foggia, Italy<br />

Antonio Bevilacqua, Maria Rosaria Corbo and Milena Sinigaglia (Eds)<br />

All rights reserved - © 2010 <strong>Bentham</strong> <strong>Science</strong> Publishers Ltd.<br />

CHAPTER 8<br />

Abstract: High pressure processing has been proposed since the beginning <strong>of</strong> the 1900, as a suitable mean<br />

for reducing <strong>food</strong> contamination by pathogens and spoiling microorganisms. It is defined as non-thermal<br />

treatment that uses the pressure (300-700 MPa, in some cases up to 1000 MPa) as the main <strong>preservation</strong><br />

method.<br />

Based on the different ways to achieve pressure increase, we can distinguish between High Hydrostatic<br />

Pressure (HHP) and High Pressure Homogenization (HPH); HHP attains pressure rise through a fluid,<br />

whereas in HPH treatments pressure increases as a consequence <strong>of</strong> forcing product through a small valve<br />

(homogenizing valve).<br />

Both these approaches have been proposed for different kinds <strong>of</strong> <strong>food</strong>s (HHP, for chopped onions, apple<br />

sauce and apple sauce/fruit blends as eat-on-to-the-go single serve tubes; HPH, for milk and juices) and<br />

currently used in many industrial <strong>application</strong>s.<br />

The chapter proposes an exhaustive description <strong>of</strong> both these methods, including the mode <strong>of</strong> actions against<br />

the microorganisms, the modifications on <strong>food</strong>stuffs, a possible combination with some other hurdles and<br />

some examples <strong>of</strong> industrial <strong>application</strong>s.<br />

Finally, in the case <strong>of</strong> HHP there is a report on its safety and implications on health, based on some<br />

publications <strong>of</strong> Public Agencies.<br />

Key-Concepts: High Hydrostatic Pressure, Homogenization, Effects <strong>of</strong> pressure on microorganisms,<br />

Equipments.<br />

HIGH HYDROSTATIC PRESSURE<br />

HIGH HYDROSTATIC PRESSURE: AN INTRODUCTION<br />

There are several definitions <strong>of</strong> the term high pressure processing; hereby, we will use the most simple, i.e. High<br />

Pressure Processing (HPP) is a non-thermal <strong>food</strong> processing, that uses the pressure (300-700 MPa, in some cases<br />

up to 1000 MPa) as the main <strong>preservation</strong> method [1]. Due to the fact that pressure increase is achieved through<br />

a fluid (for example water), this process has been also referred to as High Hydrostatic Pressure (HHP) as<br />

opposite to the High Pressure <strong>of</strong> Homogenization (HPH), where the increase <strong>of</strong> the pressure is obtained forcing<br />

the product through a small valve (homogenizing valve).<br />

Bert Hite was the first to use this method as an <strong>alternative</strong> approach for <strong>food</strong> <strong>preservation</strong>; he pressurized many<br />

kinds <strong>of</strong> <strong>food</strong>s and beverages in the late 1890s and at the beginning <strong>of</strong> the 20 th century [2]. Since these initial<br />

efforts, other researchers tried to use this approach, but only in the 1980s the suitability <strong>of</strong> HHP as a <strong>food</strong><br />

<strong>preservation</strong> method was realized [2].<br />

The first HHP-treated products (jams and jellies) appeared in 1991 in Japan; in 2001, guacamole (pressuretreated<br />

avocado) entered US marketplace, followed by HHP salsa and in 2004 by chopped onions, apple sauce<br />

and apple sauce/fruit blends as eat-on-to-the-go single serve tubes.<br />

In the EU a consumer research [3] reported a 67% <strong>of</strong> acceptability from consumers <strong>of</strong> three different European<br />

countries (France, Germany and United Kingdom), thus opening the way for the marketing <strong>of</strong> HHP-treated<br />

products.<br />

An interesting description <strong>of</strong> HHP treatment can be found in the paper <strong>of</strong> Riva [4]: We should imagine two<br />

elephants (10-12 tons) laid on a penny; the coin is subjected to a pressure <strong>of</strong> ca. 900 MPa. Now, we should<br />

image that the same pressure has been applied on a egg through water: you don’t break the egg, but you cook it<br />

*Address correspondence to this author Milena Sinigaglia at: Department <strong>of</strong> Food <strong>Science</strong>, Faculty <strong>of</strong> Agricultural <strong>Science</strong>, University<br />

<strong>of</strong> Foggia, Italy; E-mail: m.sinigaglia@unifg.it

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