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Cleanable Liquids Processing Equipment and Systems 253
FIGURE 17 This tandem sterile filter for media is in the line to the transfer panel upper right. The
U-Bend is installed to the selected bioreactor and the filter housingsand transfer line are steamed-inplace
prior to the transfer and filtration.
housings and the piping from the media vessels, via connection the end of this line
to CIPR.
Consideration of filter housing CIP came late in the subject project, and the
budget provided for only hand valves on the several filter carts. By replacing only
two manual valves with air-operated valves in astrategic manner, and opening all
other manual valves for CIP, fully automated CIP was possible at amodest added
cost (Fig. 18).
The vent and condensate piping was all sized 0.5 (12 mm) to 1.5 in. (38 mm)
header with an elbow to drain for production operations. Aportable trap was
added for SIP. A1.5-in. CIPS header was connected to the fixed sprays by flex-lines
to the two filter inlets for CIP. Following removal of the cartridges, the
reassembled housings were spray cleaned in reverse flow at 45 gpm (22–23 gpm
each) the velocity required to achieve 5ft/sec (1.5 m/sec) in the 2-in. (50 mm)
media supply line. Brief opening of V1 and V2 provided full flow through the filter
outlet header and through seven 0.5 ft (12 mm) paths of nearly equal length in
parallel. When both V1 and V2 were closed full flow was divided between the
two housings.
STEAM-IN-PLACE
The design criteria to develop aCIPable process meet most of the requirements for
SIP. Additional components for SIP might be limited to asource of CS, condensate
traps at the low drain points, and temperature sensors to monitor the SIP procedure.