2.1.8.2. Absorbency Under Load (AUL) - BADA

3.3.Ⅱ. Extent of Chemical Modification
Figure 32 shows the percentage of chemical modification of lysyl residues in isolated zygomycetes
protein (IZP) with EDTAD. The experimental work was shown, the extent of modification
increase with rising amount of EDTAD to protein ratio (w/w %). For IZP, the protein to EDTAD
ratio of 1: 0.4 was sufficient to modify 90% of the lysyl residues.
Extent of modification (%)
100
80
60
40
20
0
0 0.1 0.2 0.3 0.4 0.5
EDTAD / Protein (g/g)
Figure 32. Diagram of modification percentage of lysyl residue of IZP with EDTAD
3.4.Ⅱ. Effect of Glutaraldehyde
Figure. 33 shows the effect of glutaraldehyde (GLA) concentration as a crosslinker on the swelling
behavior of the IZP- superabsorbent hydrogel. In this experiment, increasing the concentration of
GLA from 0.01 to 0.08 M when preserving the acylated zygomycetes protein concentration at 1%
(w/w) was studied.
60