2.1.8.2. Absorbency Under Load (AUL) - BADA

Calculations
As a result by solving the following equation that was achieved from standard curve of protein
concentration, the percentage of zygomycetes protein concentration was calculated.
Y = 1.8569 X ─ 0.0133
1.27 = 1.8569 X ─ 0.0133 ⇒ X = mg of protein from the standard curve = 0.69
mg Protein = mg of protein from the standard curve = 0.69
(mg Protein)
% Protein = ───────────────── (100)
(mg solid ⁄ ml Reagent D)
0.69 mg
% Protein of zygomycetes = ───────────── (100) = % 47.7
1.446 mg ⁄ ml
3.2.Ⅱ..Determination.of Zygomycetes.Lipid.content …….
………………………….. ……………………………………..
Zygomycetes biomass lipid includes fatty acids ester. The amount of lipid present in zygomycetes
biomass was determined by solvent extraction method. To 50 ml of 5% w/v extracted protein
solution in water, the mixed solvents which contain: absolute ethanol (20 ml), diethyl ether (50ml),
petroleum ether (50ml) was added in decanter and roughly agitated. The extraction process was
repeated three times and the solvent phase was evaporated in weighed aluminum pans, next dried
in oven at 100 o C until a constant weight was achieved. According to our experimental work the
lipid content of zygomycetes fungi was 13.08 g lipid per 100 g dried biomass.
59