2.1.8.2. Absorbency Under Load (AUL) - BADA
2.1.8.2. Absorbency Under Load (AUL) - BADA
2.1.8.2. Absorbency Under Load (AUL) - BADA
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Preparation of<br />
protein solution (1%)<br />
Adjust the pH to 12, heat for<br />
60 min at 60 O C<br />
Add calculated amount of EDTAD, stir<br />
for 3h at PH 12<br />
Adjust the pH to 7, Dialyze<br />
modified protein against<br />
deionized water<br />
Lyophilization<br />
Add calculated amount of GLA 25% to the<br />
acylated protein and cure overnight<br />
Dry the gel at 40 O C<br />
Dried Protein Hydrogel<br />
Remove of bulk water<br />
and drying<br />
Precipitate with<br />
Ammonium Sulfate<br />
Cool at RT,<br />
neutralize with HCl<br />
Clarification and<br />
Concentration<br />
Alkaline treatment of RMP<br />
by heating the solution for<br />
60 minutes at 65 O C<br />
Refinery Mechanical<br />
Pulping (RMP) of<br />
Zygomycetes<br />
Grow zygomycetes on<br />
suitable medium<br />
Figure 20. Flow chart of zygomycetes protein superabsorbent hydrogel production<br />
46
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