2.1.8.2. Absorbency Under Load (AUL) - BADA
2.1.8.2. Absorbency Under Load (AUL) - BADA
2.1.8.2. Absorbency Under Load (AUL) - BADA
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Preparation of<br />
protein solution (1%)<br />
Adjust the pH to 12, heat for<br />
60 min at 60 O C<br />
Add calculated amount of EDTAD, stir<br />
for 3h at PH 12<br />
Adjust the pH to 7, Dialyze<br />
modified protein against<br />
deionized water<br />
Lyophilization<br />
Add calculated amount of GLA 25% to<br />
the acylated protein, cure overnight<br />
Dry the gel at 40 O C<br />
Dried Protein Hydrogel<br />
Figure 19. Flow chart of albumin superabsorbent hydrogel production<br />
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