2.1.8.2. Absorbency Under Load (AUL) - BADA

More documents

Recommendations

Info

Figure 17. Schematic of device used for the <strong>AUL</strong> measurement 2.1.9. Instrumental Analysis Infrared Analysis (FT- IR) IR spectrophotometery is a principal instrument for identification and characterization of chemical materials, especially polymeric materials [24] . One of the important characteristic of IR spectrophotometery is the study of molecular structure or functional groups that can be determined based on the vibrational motions of atoms within the molecule after interaction with electro magnetic radiation [18] , which is called the absorption spectrum of the materials. Infrared spectroscopy is a simple and rapid instrumental technique which is normally used for the identification of raw materials, intermediates and final products in analytical and quality control laboratories. In the present study, the infrared spectroscopy (FT-IR) analysis was investigated to confirm the various functional groups and chemical structures of unmodified, EDTAD-modified proteins, as well as EDTAD-AP and IZP hydrogel. 41
3 RESULT AND DISCUSSION The important part of present study is that through chemical modification of lysyl residues with EDTAD by introducing a number of carboxyl groups into protein molecules. It should be mentioned that EDTAD is a bifunctional reagent which is able to acylate of polypeptides either inter or intramolecularly. Theoretically, one molecule of EDTAD reacts with one lysyl residue which is shown in Figure 18. In this reaction three carboxyl groups can be incorporated for each lysyl residue modified, into the protein molecule [15] . This completely improves the net anionic charge of the protein with various sites for water binding, which causes in unfolding the protein structure. Crosslinking of EDTAD-modified protein with cross-linking agents such as glutaraldehyde should be produced the chemically-crosslinked protein hydrogel with superabsorbent properties. Moreover, crosslinking is rather performed in aqueous solution in order to develop both intera and inter molecular bonding for immobilizing the modified protein in the aqueous solutions. The ideal reaction pathway for the reaction of protein with EDTAD and GLA is shown in Figure 18. In the present work, after crosslinking with GLA and before drying, the protein superabsorbent hydrogels were treated with polar organic solvents. Organic solvents are able to remove any residual GLA and breakdown undesirable bonds between GLA and hydroxyl groups of amino acid chains, so the hydrophilic hydroxyl groups attend to absorb water molecules. In detail, the effect of ethanol, isopropanol and acetone for increasing the swelling capacity of protein hydrogel was investigated. 42
Page 2 and 3: BIOSUPERABSORBENT FROM PROTEINS Sar
Page 4 and 5: ACKNOWLEDGMENTS We would like to ap
Page 6 and 7: CONTENTS ACKNOWLEDGMENTS ………
Page 8 and 9: 3.4.Ⅰ. Kinetic of Swelling……
Page 10 and 11: 22- Effect of Glutaraldehyde concen
Page 12 and 13: 1 INTRODUCTION Superabsorbent hydro
Page 14 and 15: 1.1. Definition of Superabsorbency
Page 16 and 17: 1.3.1. Protein Structure Protein fo
Page 18 and 19: 2- Side chains of polar residues wi
Page 20 and 21: 1.3.3. Ovalbumin , Good Source for
Page 22 and 23: One of the paper preparation proces
Page 24 and 25: ……………………………
Page 26 and 27: 1.5. The Thermodynamic of Protein F
Page 28 and 29: In order to chemical modification o
Page 30 and 31: The swollen crosslinked polymer may
Page 32 and 33: 1.7.1.3. Crosslinking by UV-Irradia
Page 34 and 35: 1.7.2.1. Non-specific Crosslinking
Page 36 and 37: 1.8. Determination the Degree of cr
Page 38 and 39: These preparations contain consider
Page 40 and 41: MATERIALS AND METHODS 2.1. Method o
Page 42 and 43: ased on the solubility of the prote
Page 44 and 45: 2.1.2.2. Determination of Zygomycet
Page 46 and 47: 2.1.3. Modification of proteins Alb
Page 48 and 49: The first step was weighted a 30 mg
Page 50 and 51: 2.1.8.1. Centrifuge Retention Capac
Page 54 and 55: Results showed the effect of ethano
Page 56 and 57: Preparation of protein solution (1%
Page 58 and 59: PART Ⅰ ALBUMIN SUPERABSORBENT HYD
Page 60 and 61: 3.3.Ⅰ. Effect of Buffer solution
Page 62 and 63: Swelling (g/g) 300 200 100 0 1 0.01
Page 64 and 65: Swelling (g /g ) 350 300 250 200 15
Page 66 and 67: 3.8.Ⅰ. Effect of Temperature Prot
Page 68 and 69: In this rearrangement the R─ grou
Page 70 and 71: Calculations As a result by solving
Page 72 and 73: In the present work, increasing the
Page 74 and 75: In detail, Figure 36 shows the comp
Page 76 and 77: Sample Table 5. Comparison of swell
Page 78 and 79: The IZP-hydrogel compared with unmo
Page 80 and 81: According to our results, the swell
Page 82 and 83: REFERENCES 1- Fredric L.Buchholz &
Page 84 and 85: 32- Alexander,. J, Colloid chemistr

2.1.8.2. Absorbency Under Load (AUL) - BADA

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?