2.1.8.2. Absorbency Under Load (AUL) - BADA
2.1.8.2. Absorbency Under Load (AUL) - BADA
2.1.8.2. Absorbency Under Load (AUL) - BADA
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In order to preparation of Biuret reagent (C), all C components in above Table were dissolved in<br />
100 ml 0.2 M NaOH then increased volume to 250 ml with deionized water by volumetric flask<br />
and if a black precipitate forms, it should be thrown away.<br />
Pipette (in ml) the following reagents into suitable containers:<br />
Reagent Test STD 1 STD 2 STD 3 STD 4 STD 5 Blank<br />
A (NaCl) ─ 0.96 0.9 0.8 0.4 ─ 1.00<br />
B (WPS) ─ 0.04 0.1 0.2 0.6 1.00 ─<br />
D (Protein) 1.00 ─ ─ ─ ─ ─ ─<br />
Mix by swirling then add (C)<br />
C (Biuret) 4.00 4.00 4.00 4.00 4.00 4.00 4.00<br />
Mix above samples thoroughly by vortexing and incubate for 30 minutes at 25°C. Transfer to<br />
suitable cuvettes and record the absorbance at 540 nm for Test, Standards, and Blank. The standard<br />
curve absorbance measurement of zygomycetes protein is shown in Figure 30 (Part 3.1.Ⅱ.).<br />
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