2.1.8.2. Absorbency Under Load (AUL) - BADA

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ACKNOWLEDGMENTS We would like to appreciate all the people who made this work possible. First of all, we would like to thank our supervisor, Professor Mohammad Taherzadeh, for the scientific guidance and constructive advices that we received from him. We wish to thank Akram Zamani for friendly help and share equipments at Biotechnology research laboratory. We would also like to thank Jonas Hanson for helping us throughout this experimental work at Chemistry laboratory. We would also like to give special thanks to our parents (Mr. & Mrs. Majd) for kindly support and encouragement during our study from far distance and thanks to Amirabbas Majd for cover design of our thesis work and his deep kindness. Ⅲ
ABSTRACT The present work is synthesizing novel protein-based superabsorbent hydrogels from albumin protein (AP) and isolated zygomycetes protein (IZP) via modification with an acylating reagent and a bifunctional crosslinker, also investigation on their swelling behaviors under some conditions. ………………… ………………………………………………………………….. The hydrophilic acylating reagent was introduced into albumin and zygomycetes proteins and hydrophylicity of albumin and isolated zygomycetes protein were increased by ethylenediaminetetraacetic dianhydride (EDTAD). Provided protein hydrogels through this method include modification of lysyl residues in the unfolded proteins via adding of one or more hydrophilic carboxyl groups. The reaction was developed with a dialdehyde crosslinking reagent (glutaraldehyde) in order to stabilize the modified protein configuration. The maximum capacity of EDTAD-AP hydrogel swelling was observed 296 g water per g of dry gel, and for EDTAD- IZP hydrogel was 87 g water per g of dry gel. In this study, effect of selected physical and chemical parameters such as protein structure, extent of modification, protein concentration, various pH, ionic strength, gel particle size, temperature as important factors affecting the water uptake behavior of superabsorbent hydrogel were investigated. In addition, the effect of some organic solvents particularly absolute ethanol for increasing the swelling properties was studied. KEY WORDS: Superabsorbent, Hydrogel, Albumin protein, Isolated zygomycetes protein, Chemical modification, Crosslinking agent, Swelling behavior, Biodegradability. Ⅳ
Page 2 and 3: BIOSUPERABSORBENT FROM PROTEINS Sar
Page 6 and 7: CONTENTS ACKNOWLEDGMENTS ………
Page 8 and 9: 3.4.Ⅰ. Kinetic of Swelling……
Page 10 and 11: 22- Effect of Glutaraldehyde concen
Page 12 and 13: 1 INTRODUCTION Superabsorbent hydro
Page 14 and 15: 1.1. Definition of Superabsorbency
Page 16 and 17: 1.3.1. Protein Structure Protein fo
Page 18 and 19: 2- Side chains of polar residues wi
Page 20 and 21: 1.3.3. Ovalbumin , Good Source for
Page 22 and 23: One of the paper preparation proces
Page 24 and 25: ……………………………
Page 26 and 27: 1.5. The Thermodynamic of Protein F
Page 28 and 29: In order to chemical modification o
Page 30 and 31: The swollen crosslinked polymer may
Page 32 and 33: 1.7.1.3. Crosslinking by UV-Irradia
Page 34 and 35: 1.7.2.1. Non-specific Crosslinking
Page 36 and 37: 1.8. Determination the Degree of cr
Page 38 and 39: These preparations contain consider
Page 40 and 41: MATERIALS AND METHODS 2.1. Method o
Page 42 and 43: ased on the solubility of the prote
Page 44 and 45: 2.1.2.2. Determination of Zygomycet
Page 46 and 47: 2.1.3. Modification of proteins Alb
Page 48 and 49: The first step was weighted a 30 mg
Page 50 and 51: 2.1.8.1. Centrifuge Retention Capac
Page 52 and 53: Figure 17. Schematic of device used
Page 54 and 55:
Results showed the effect of ethano
Page 56 and 57:
Preparation of protein solution (1%
Page 58 and 59:
PART Ⅰ ALBUMIN SUPERABSORBENT HYD
Page 60 and 61:
3.3.Ⅰ. Effect of Buffer solution
Page 62 and 63:
Swelling (g/g) 300 200 100 0 1 0.01
Page 64 and 65:
Swelling (g /g ) 350 300 250 200 15
Page 66 and 67:
3.8.Ⅰ. Effect of Temperature Prot
Page 68 and 69:
In this rearrangement the R─ grou
Page 70 and 71:
Calculations As a result by solving
Page 72 and 73:
In the present work, increasing the
Page 74 and 75:
In detail, Figure 36 shows the comp
Page 76 and 77:
Sample Table 5. Comparison of swell
Page 78 and 79:
The IZP-hydrogel compared with unmo
Page 80 and 81:
According to our results, the swell
Page 82 and 83:
REFERENCES 1- Fredric L.Buchholz &
Page 84 and 85:
32- Alexander,. J, Colloid chemistr
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2.1.8.2. Absorbency Under Load (AUL) - BADA

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