Cover Page - AquaFish CRSP

AQUAFISH INNOVATION LAB
PROPOSAL SUMMARY PAGE FORM
Title of Proposal:
Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and Tanzania
Development Theme (select only one)
X
A Improved Health and Nutrition, Food
Quality, and Food Safety
C Environmental Management for
Sustainable Aquatic Resources Use
B Income Generation for Small-Scale Fish D Enhanced Trade and Investment for
Farmers
Global Fishery Markets
Names and institutional affiliations of Principal Investigator(s) and Co-PIs submitting this proposal:
US co-PI: Emmanuel A. Frimpong, Virginia Polytechnic Institute & State University, Blacksburg,
VA USA
US co-PI: Rebecca Lochmann, University of Arkansas at Pine Bluff, USA
US co-PI: Maria Haws, University of Hawaii at Hilo, USA
HC PI: Stephen Amisah, Kwame Nkrumah University of Science & Technology, Kumasi, Ghana
HC co-PI: Nelson W. Agbo, Kwame Nkrumah University of Science & Technology, Kumasi, Ghana
HC co-PI: Regina Edziyie, Kwame Nkrumah University of Science & Technology, Kumasi, Ghana
HC co-PI: Gifty Anane-Taabeah, Kwame Nkrumah University of Science & Technology, Kumasi,
Ghana
HC PI: Sebastian Chenyambuga– Sokoine University of Agriculture, Tanzania
HC collaborator: Nazael Madalla – Sokoine University of Agriculture, Tanzania
HC collaborator: Hieromin A. Lamtane – Sokoine University of Agriculture, Tanzania
HC collaborator: Fulgence J. Mishili – Sokoine University of Agriculture, Tanzania
HC PI: Narriman Jiddawi, Institute of Marine Sciences (IMS), University of Dar es Salaam,
Zanzibar, Tanzania
HC co-PI: Julius Francis, Western Indian Ocean Marine Sciences Association (WIOMSA), Zanzibar,
Tanzania
Seven investigations per proposal is recommended. Maximum of 10 investigations per proposal.
Proposals must include at least one experiment or study. Proposals must also include at least one
outreach activity that focuses on women and/or girls. See the RFP for details.
1.
2.
3.
4.
Title of
Investigation
Assessing the Nutritional
Impact of Aquaculture Policy
in Fish Farming Districts in
Tanzania
Development of a Cell-Phone
Based Seafood Market
Information System (SMIS) in
Ghana: Application to Tilapia
Value Chain Analysis of
Farmed Nile Tilapia
(Oreochromis niloticus) and
African Catfish (Clarias
gariepinus) in Tanzania
Spat Collection and Nursery
Methods for Shellfish Culture
by Women
Topic Area
(RFP p. 3-4)
(one per
investigation)
3
TYPE OF
INVESTIGATION
EXPERIMENT/
STUDY/ ACTIVITY
Total
Cost
(US $)
Host
Country
Institutions
Involved
PDV Study 96,000 Tanzania
MER Study/Activity 114,000 Ghana
MER Study/Activity 69,000 Tanzania
HHI Experiment 91,000 Tanzania

5.
6.
7.
8.
Coastal Women’s Shellfish
Aquaculture Development
Workshop
Identifying Local Strains of
Oreochromis niloticus that are
Adapted to Future Climate
Conditions
Assessing Ghana’s National
Aquaculture Training Needs
and Capabilities and Climate
Change Preparedness in the
Light of the National
Aquaculture Development
Plan
Enhancing the Nutritional
Value of Tilapia for Human
Health
HHI Activity 46,000 Tanzania
IND Experiment 115,000 Ghana
PDV Study 98,000 Ghana
STF Study 121,000 Ghana
4
Sum: $750,000
1 The sum of the costs of all investigations should equal the total cost of federal funds requested on the cover sheet.
032613

Executive Summary
Introduction:
African governments acknowledge in National Development Plans that urgent poverty reduction
measures are needed to achieve the UN Millennium Development Goals, with governments most
focusing on national poverty eradication strategies and improvements in food nutrition and
security. Fish has always been an important part of the diet of the people of the continent but
until recently fish has been largely harvested from the wild. Total fish output in some African
nations such as Nigeria and Egypt continue to grow at accelerating rates and fish cultivation has
become part of many rural agricultural enterprises. This has been encouraged by expansion of
NGO developmental activities on aquaculture, improved aquaculture production technologies,
recognition of over exploitation of natural fisheries, and increased nutritional requirement of a
rapidly growing population. These factors combine to make aquaculture an economically
attractive agricultural production alternative in sub-Saharan Africa.
Previous AquaFish CRSP work has focused on a broad range of issues targeting poverty
reduction and increased productivity. In Tanzania, for example, lower costs feed alternatives
were identified from leguminous tree species to replace the more costly soybean meal used in
tilapia diets. Through training in hatchery techniques and management, Kenya farmers now have
an additional aquaculture enterprise of raising catfish fingerlings and selling as bait to the
longline fishing industry on Lake Victoria. Traders in baitfish from natural catches are now
engaged in fish farming that has enabled year-round supply of baitfish. Training in supply chain
management has enabled the development of new market opportunities for foodfish producers in
Kenya and Ghana as well as new markets for baitfish producers in Kenya. Farmers have acquired
knowledge about the environmental effects of their activities and are therefore implementing
broadly focused environmental BMPs on their farms, especially in Ghana and Kenya. Studies
that analyzed tilapia value chain provided valuable information needed for the improvement of
tilapia trade through market intelligence. Similarly, consumer preference studies for farmed fish
provided information for the development of consumer-driven aquaculture production in Ghana
and Kenya.
The vision of this project is to build on previous work to enhance the profitability of the
aquaculture industry in sub-Saharan Africa through physical and human capacity development;
enhanced market information sharing and trading; improved nutritional qualities of fish and
consequently human nutrition; growth of a whole chain of activities from farm to the consumer;
better management of native fish and shellfish species. Results from the various investigations
will help to achieve the goals of improving human nutrition, efficiency in the value chain,
increased incomes for producers and traders of aquaculture products, diversified production
systems, enhanced nutrient, and reduction in postharvest losses through efficient market
information sharing mechanisms.
This project involves knowledge generation and physical and human capacity
development. Student participation in research activities is designed to create a framework such
that all additional materials and investment by this project are viewed as part of quality
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improvement of the Aquaculture Sciences program. Involvement of students means involving a
set of other faculties such as graduate committee members. This will facilitate joint planning and
management of the additional equipment and investments and also create continuity, as other
students outside the program will continue to use the additional facilities.
2

Investigation #1
Title: Assessing the Nutritional Impact of Aquaculture Policy in Fish Farming Districts in
Tanzania
Topic Area: Policy Development (PDV)
Investigation: Study/Activity
Lead PI: Kwamena K. Quagrainie, Purdue University, West Lafayette, IN, USA
HC PI: Elibariki Emmanuel Msuya – Sokoine University of Agriculture
Objectives:
1. Assess improvements in household food security in selected fish farming communities.
2. Measure the effect of aquaculture as an input and technology transfer program on
household food security using nutritional indicators.
Significance:
Aquaculture is one of the world’s fastest growing food production sectors with great potential for
food supply, poverty alleviation and enhanced trade and economic benefits. Fish farming has
contributed towards poverty alleviation in poor societies in various areas of the world where it is
traditionally practiced, e.g., Bangladesh (Jahan et al., 2010) and Nepal (Bhujel et al., 2008).
Aquaculture development in Sub-Saharan Africa aims at improving food security and human
nutrition; increasing domestic fish production; creating employment; promoting diversification
and reducing risk; promoting economic development; and improving efficiency in the use of
resources, especially water (Béné and Heck, 2005; Satia, 2011).
Majority of Tanzania’s population live in rural areas; approximately 80% live below the
national poverty level (less than US$1.00 per day) and therefore suffer from under nutrition. In
recent years food security has emerged as the main challenge for the Tanzanian government to
achieve the target of the United Nations Millennium Development Goals, (MDGs). Aquaculture
is considered as an option for rural development because it provides an important opportunity to
help solve problems of poverty, and protein malnutrition of the rural poor. Aquaculture
expansion in Asian countries like Bangladesh and Thailand has led to enhanced food security
among adopters and the population at large (De Silva and Davy, 2010; Jahan et al., 2010; Lazard
et al., 2010). Bueno (2009) reported that the fish farmer’s goal is often to produce the family
animal protein food supply and sell part of the harvest for additional family income.
Tanzania’s national development objectives for aquaculture include generating income
for rural communities, increasing employment opportunities, improving rural quality of life,
specifically through raising the living standards by improving food and nutritional security, and
minimizing threats to food insecurity (URT, 1997). The focus of this study is to look beyond the
direct production outcomes from fish farming in rural communities and consider the additional
benefits that manifest themselves in nutritional outcomes. Accounting for changes in nutritional
outcomes is particularly relevant in Tanzania given the efforts of the Tanzania government to
improve nutritional security.
Methodology:
3

The anthropometric literature abounds with various indicators that measure nutrition-based food
security. Anthropometrics is a means to measure human welfare in relation to changes in food
supply and other development processes. Thus individual characteristics as well as household
and community variables help to determine nutrition status. National policies on social and
economic issues also contribute to the nutritional outcomes of various populations (Madise et al.
1999). Individual level characteristics is reported to affect nutritional status as it is a reflection of
dietary intake, illness, age, gender, size of baby at birth and whether the child is breastfed or not
(Sahn and Alderman 1997; Madise et al. 1999; Chirwa and Ngalawa 2008). Also, household
level characteristics such as the level of consumption and income; the gender and age of the
household head; the age, educational and nutritional status of the mother; land size and the
production of own food are reported to influence anthropometric outcomes (Quinn et al. 1990;
Madise et al. 1999; Tharakan and Suchindran 1999; Chirwa and Ngalawa 2008).
Data & Analysis
We follow the anthropometric literature to assessing the nutritional impact of aquaculture policy
in selected fish farming districts in Tanzania. Data for the study will be obtained from Tanzania’s
Household Budget Survey (HBS) and supplemented with household cross-sectional data from
Sokoine University of Agriculture. The data set is a cross-sectional data that comprises
characteristics of communities and household level data. The HBS collects information on a
wide range of households and individual characteristics such as household members’ education,
economic activities, health status, household expenditure, consumption and income, ownership
of assets and consumer goods, housing structure and building materials, distance to services and
facilities and food security. Data is collected on 216 food item categories, which includes fresh
fish, shellfish, fresh dried fish, dried or salted fish, and canned fish.
Households will be selected from the southern highlands and northern highlands in
districts where fish farming is predominantly practiced. Villages will be selected based on the
intensity of aquaculture activities. Since HBS data includes community characteristics, their
effects will be controlled by complementing it with the information at the household level.
The assessment will measure nutritional impact through a series of regression analysis
which would include whether or not households have participated in aquaculture and other
aquaculture-related programs. The comparison of participation in aquaculture will be
accomplished under the assumption of random placement to minimize selection bias. However,
where specific villages or communities were targeted for aquaculture programs and activities,
non-random treatments will be adopted in the regression analysis.
Deliverables:
Ø Information on any relationships between household nutrition factors and participation in
aquaculture activities.
Ø Potential nutritional payoff or gains from national policy that encourages aquaculture
technology transfer and adoption.
Ø Indicator measures that capture aspects of aquaculture’s impact on human nutrition and food
security.
Quantifiable Benefits:
Ø Results will inform important policy decisions and implications on developing aquaculture in
Tanzania.
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Ø Documentation of government expenditures on aquaculture development and the effects on
addressing food security and household welfare.
Ø Aquaculture development will be viewed not only as improving food production and rural
employment but also food security of households through improved nutrition.
Schedule:
Data collection and collation July - December 2013
Data Analysis January - September 2014
Write up and Reporting October - September 2015
References:
Béné, C. and Heck, S. (2005). “Fish and food security in Africa.” NAGA, World fish Centre
Quarterly report, 28: 8-13.
Bhujel, R.C., Shrestha, K., Pant, J and Buranrom, S. (2008). “Ethnic women in aquaculture in
Nepal”. Development, 51: 259-264.
Bueno, P.B. (2009). “Indicators of sustainable small scale aquaculture development” In: Bondad-
Reantaso M.G. and Prein, M. (eds). Measuring the contribution of small scale
aquaculture: an assessment. FAO Fisheries and Aquaculture Technical Paper, No. 534,
pp 145-160.
Chirwa, E.W. and Ngalawa, H.P. (2008). Determinants of Child Nutrition in Malawi, South
African Journal of Economics 76, 628-640.
De Silva, S.S. and Davy, E.B. (2010). “Aquaculture success in Asia: Contributing to sustained
development and poverty alleviation” In: De Silva, S. and Davy, F.B (eds) Success
stories in Asian aquaculture. Springer, International Development Research Centre pp 1-
14.
Jahan, K. M-e., Ahmed, M. and Beltom, B. (2010). “The impacts of aquaculture development on
food security: lessons from Bangladesh”. Aquaculture Research, 41:481-495.
Lazard, J., Baruthio, A., Mathe, S., Rey-Valette, H., Chia, E., Clement, O., Aubin, J., Morissens,
P., Mikolasek, O., Legendre, M., Levang, P., Blancheton, J. and Rene, F. (2010).
“Aquaculture system diversity and sustainable development: fish farms and their
representation”. Aquatic Living Resources, 23: 187-198.
Madise, N.J., Matthews, Z. and Margetts, B. (1999). Heterogeneity of child nutritional status
between households: A comparison of six sub-saharan African countries, Population
Studies 53, 331-343.
Quinn, V., Chiligo, M. and Gittinger, J.P. (1990). Malnutrition, household income and food
security in rural Malawi, Health Policy and Planning 5, 139-148.
Sahn, D.E. and Alderman, H. (1997). On the Determinants of Nutrition in Mozambique: The
Importance of Age-Specific Effects, World Development 25, 577-588.
Satia (2011). Regional review on status and trends in aquaculture development in Sub-Saharan
Africa – 2010. FAO, Rome 214p.
Tharakan, C.T. and Suchindran, C.M. (1999). Determinants of child malnutrition-An
intervention model for Botswana, Nutrition Research 19, 843-860.
URT. (1997). National Fisheries sector policy and strategy statement. Ministry of Natural
resources and Tourism, Dar es Salaam, Tanzania.
5

Investigation #2
Title: Development of a Cell-Phone Based Seafood Market Information System (SMIS) in
Ghana: Application to Tilapia
Topic Area: Marketing, Economic Risk Assessment, and Trade (MER)
Investigation: Study/Activity
Lead PI: Kwamena Quagrainie, Purdue University, West Lafayette, IN, USA
HC PI: Stephen Amisah, Kwame Nkrumah University of Science & Technology,
Kumasi, Ghana
Collaborator: Alloysius Attah, FarmerLine, Ayeduase Street, Kumasi, Ghana.
Objectives:
3. Develop an electronic system for tilapia market information exchange.
4. Develop a phone-based market information sharing platform for fish producers,
fishermen, seafood marketers, and consumers.
5. Train fish farmers, fishermen, women fish processors, markets and traders on the use of
the market information system developed under 1 & 2 to enhance trade and profitability.
Significance:
An analysis of tilapia value chain in Ghana under a previous AquaFish CRSP project revealed
challenges in the flow of information along the value chain, especially information relating to
tilapia supply, demand and prices. For small-scale fish producers and artisanal fishermen, readily
available market information on prices and demand for tilapia at different fish markets will help
inform production and harvesting decisions. Minimizing the information gaps along the tilapia
value chain will greatly improve efficiencies in tilapia marketing and the value chain as a whole.
There is a need therefore to develop a marketing information system for tilapia in Ghana. A
market information system involves processes to generate, store, analyze, and disseminate
marketing information on a regular basis. A similar system is in use in Indonesia (InfoFish, 2010,
2008) and Kenya (KMFRI, 2010). In Indonesia, the Fish Marketing Information System provides
a platform for a transparent and fair fish trading and improves market access for fish products
from Banda Aceh to regional markets (InfoFish, 2010).
A seafood marketing information system (SMIS) in Ghana will be useful for efficient
operation of the tilapia value chain. Opportunities for improved communication and increased
information flow along the tilapia value chain exist in Ghana through the use of mobile phone
technology. Mobile phone penetration in Ghana is 94%, making this an ideal channel linking fish
producers and artisanal fishermen with markets. Mobile-based service opens these
communication pathways and allows market data and information to be programed and can be
easily accessed by users from a mobile device via voice and/or short messaging service (SMS)
anytime. These services could result in increases in fish quality and yield as well as an increase
in incomes of farmers and traders. An efficient market information system via the mobile
technology has an important role to play in improving aquaculture productivity and value chain
efficiency in Ghana. A marketing information system for tilapia in Ghana will results in a more
organized tilapia market data collection, the storage of important tilapia market data, better
coordinated marketing intelligence information, and access to market information to make
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usiness decisions. It will also assist in building capacity to improve the skill of stakeholders on
fish marketing.
Methodology:
The first step will involve identifying various points of tilapia fish supply and demand in Greater
Accra, Eastern, and Ashanti regions in Ghana. A visit to selected supply and demand centers will
help to identify important variables for which data and information will be collected, e.g.,
quantity, prices, trends, etc. These will be collected from the various stakeholders including fish
producers, artisanal fishermen, middlemen, fish marketers and retailers. Some key individuals or
point persons will be identified at these locations to collect the data and information.
The services of a programming company, Farmerline will be used to program an
electronic information system into which the data and information collected from the supply and
demand centers will be transmitted, creating a database/platform. The system will be set up in a
form that can easily be accessed by users from a mobile device via voice and/or SMS anytime
(Figure 1).
Figure 1: A Schematic Diagram of Seafood Marketing Information System (SMIS)
Partners and Content Providers
• AquaFish Innovation Lab
Personnel
• Ministry of Fisheries
• Fisheries Commission
• Water Research Institute
• Fish Farmers & Fishermen
• Seafood Traders & Retailers
• Other stakeholders
SEAFOOD MARKETING INFORMATION SYSTEM
Data &
Information
Input
Marketing
Information
Exchange
Platform
Targeted features of the system include the ability to query for particular kinds of information;
communicate with large numbers of users; and messages received in native languages. Users will
dial or text to a number and will receive messages through either voice or SMS on key market
information in the database. Queries can be made for market information at particular locations.
The SMIS will be web-based and will also provide fish market information on-line in addition to
via voice/SMS to stakeholders.
Detailed statistics can be obtained on the number of users and how messages are
received. The system will also include a tool that allows the conduct of immediate or
longitudinal surveys with users. The survey tool will include recorded questions and users would
answer by simply pressing buttons on their phone, and their responses are recorded immediately.
The new technology will be tested with fish farmers, fishermen and seafood traders.
There will be two training programs on how to use mobile phones to relay/receive information
on prices and other market data to/from the platform. Fish farmers and fishermen will be trained
separately from the fish traders. The later training will target women seafood traders.
Deliverables:
1. A database of tilapia quantities, prices and other market information
7
Voice / SMS
Query &
Information
Flow
Users / Stakeholders
• Farmers &
Fishermen
• Seafood Traders &
Retailers
• Others

2. An electronic platform for tilapia market information sharing for producers, marketers,
and consumers
3. Enhanced tilapia trade activities
Beneficiaries:
Fish farmers, fishermen, fish processors, seafood traders and retailers, policy makers, academia,
etc.
Quantifiable Benefits:
1. Detailed statistics on stakeholder use of SMIS.
2. One-stop shop for organized market data on tilapia. This will provide resources for the
development of marketing plans and strategies as well as inform policy.
3. Access to sufficient market information and market intelligence that are needed for informed
decisions.
4. Database assembled over a period of time will be available for any quantitative analysis of
the tilapia market in Ghana.
Future Plans:
SMIS will become a pay-per-use system because of airtime minutes. Pricing arrangements will
be made with mobile phone companies. Prices will take into account the cost of system
maintenance, secured server services, audio recording in local languages and programming
support. In the long-run, the system should be self-supporting.
Schedule:
a) Identify points of fish supply and demand
b) Identify selected variables for which data and
information will be collected, e.g., quantity, prices,
July - December 2013
trends, etc
c) Identify key individuals to transmit data and
information to the database
Programming the electronic information platform January - July 2014
a) Data and information collection from the supply and
demand points to a data center.
b) Data and information programming into the system
May 2014 – September 20115
and set up in a form that can be easily accessed by
users from a mobile device via voice and/or SMS
anytime.
Testing of the Seafood Market Information System
October – December 2014
(SMIS) & fine-tuning the platform
Training fish farmers & artisanal fishermen in the use of
February 2015
SMIS
Training women fish processors, markets and traders in
March 2015
the use of SMIS
Launch and Reporting April - September 2015
8

References:
InfoFish. 2010. Workshop on Fish Marketing Information System in Indonesia. InfoFish -
Fishing Technology Digest for Asia-Pacific, Issue 70, April-June 2010 pp. 6.
InfoFish. 2008. SMS-Based Fish Marketing Information System Launched. InfoFish
International, Vol 5, pp. 52.
Kenya Marine and Fisheries Research Institute – KMFRI. 2010. Enhanced Fish Market
Information System. Aquanews - KMFRI newsletter, Vol 1, Issue 1. April 2010, pp 7.
9

Investigation #3
Title: Value chain analysis of farmed Nile tilapia (Oreochromis niloticus) and African catfish
(Clarias gariepinus) in Tanzania
Topic: Marketing, Economic Risk Assessment, and Trade (MER)
Type of investigation: A study
INVESTIGATORS AND INSTITUTIONS:
Lead PI: Kwamena Quagrainie – Purdue University
HC PI: Sebastian W. Chenyambuga – Sokoine University of Agriculture, Tanzania
Collaborators Nazael Madalla – Sokoine University of Agriculture, Tanzania
Elibariki Emmanuel Msuya – Sokoine University of Agriculture, Tanzania
Objectives
The overall goal of this study is to characterize and identify areas of improvement in value chain
of Nile tilapia and African catfish in Tanzania. Specific objectives are:
1. To identify and map various actors currently involved in Nile Tilapia and African catfish
value chain and their functions;
2. To analyze marketing margins of the different sub-sectors of Nile Tilapia and African catfish
value-chain as the produces move from producers downstream the value chain;
3. To identify the key constraints and problems affecting different actors in the value-chain;
4. To build capacity and organize fish farmers into registered groups.
Significance of the study:
In Tanzania Fish farming is currently being emphasized as an alternative to capture fisheries due
to decline in wild stock from natural water bodies. The emphasis of the national fisheries policy
(URT, 1997) is on a semi-intensive integrated mode of fish culture, focusing on Nile tilapia. The
Nile tilapia is given first priority due to its better characteristics that include fast growth, short
food chain, efficient conversion of food, high fecundity (which provides opportunity for
distribution of fingerlings from farmer to farmer), tolerance to a wide range of environmental
conditions, and good product quality (Hussain et al., 2000; Neves et al., 2008). Another species
that is given priority is the African catfish (Clarias gariepinus). The African catfish is either
cultured on pure stand or in polyculture with Nile tilapia. In the polyculture system the African
catfish is used as a predatory fish species that eats the surplus fry, hence, controls undesirable
tilapia recruitment in ponds and permits better growth of the adult Tilapia population. At the
moment aquaculture is dominated by freshwater fish farming in which small-scale farmers
practice both extensive and semi-intensive fish farming. It is estimated that the fisheries sector
contribute 1.6% of the national GDP and about 27% of the animal protein consumed in the
country. Unfortunately, the country statistics do not distinguish between aquaculture and wild
capture as the source of fish. It is therefore, difficult to draw firm conclusions about the
proportion of total fish consumed or traded from aquaculture.
In Tanzania the demand for products from aquaculture is predicted to increase due to
population growth, expected economic development and changes in eating habits coupled with
decline in capture fisheries. This provides opportunities for improvement of fish production and
commercialisation of the smallholder production system. Thus, there is a need to improve fish
production from aquaculture to complement the capture fisheries. Before embarking on
10

improvement of productivity of fish farming, it is important to assess the production performance
of the species cultured and economic profitability of fish farming enterprises in order to ascertain
its usefulness for poverty alleviation. Information on the productivity and profitability of smallscale
aquaculture in Tanzania is lacking. Moreover, there is no adequate information on the
contribution of fish farming to household income and food security, and the national economy in
general. The contribution of aquaculture to people’s livelihoods is a key indicator for describing
the success story of an aquaculture production system. If aquaculture is to be improved and
expanded to meet the demand of fish by the growing population, considering the declining trend
of the capture fisheries from oceans and inland water sources, it is necessary to document its
contribution to human welfare and analyze each step (production, processing, distribution and
delivery to final consumers) in relation to the preceding steps and the steps that follow.
Value chain analysis is the key entry point to poverty alleviation and achieving pro-poor
outcomes. It is usually aimed at increasing the total amount and value of products that the poor
can sell in the value chain (Hempel, 2010). This, in turn, results in higher absolute incomes for
the poor as well as for the other actors in the value chain. The other objective of value chain
analysis is to sustain the share of the poor in the sector or increase the margins per product, so
that the poor do not only gain more absolute income but also relative income compared to the
other actors in the value chain (Berg et al, 2008). Furthermore, value chain is used as an
analytical tool for understanding the policy environment which enable efficient allocation of
resources within the domestic economy to maximize value, prevent post-harvest losses, and
ensure effective management is in place to promote sustainable utilization of the resources.
Value chain analysis is done by mapping the actors participating in the production, distribution,
marketing and sales of particular product (or products). The mapping involves assessment of the
characteristics of actors, profits and costs structures, flow of goods throughout the chain,
employment characteristics and the destination and volumes of domestic and foreign sales,
identifying the distribution of benefits of actors in the chain, the role of upgrading and
governance within the chain. In Tanzania little is known about the value chain of cultured Nile
Tilapia and African catfish due to fact that no thorough study has been conducted on the subject
matter, thus making the government put little effort to promote fish farming for poverty
alleviation.
This study will employ value chain analysis (VCA) methodology. This is a holistic
approach which, unlike conversional methods, assesses all key actors and their functions in the
subsector from input supply, production, and postharvest to marketing. The study will involve
collection of relevant data from all key players involved in generation and flow of product from
the producers to ultimate destination and flow of value in the opposite direction. This will
provide information that will help to identify policy issues that may be hindering or enhancing
the functioning of the chain and also the areas that need improvement in the chain. In this way
aquaculture production can be improved and contribute to poverty alleviation and food security
effectively.
The proposed study intends to determine market channels of farmed fish and constraints
facing fish farming in rural areas. In addition, the study will examine the contribution of smallscale
aquaculture enterprises to income and food security of households in rural areas. This
information will help in developing appropriate improvement programmes aiming at improving
the productivity of fish farming in rural areas. This study will provide baseline information on
the importance of aquaculture to small-scale farmers and the country’s economy and will form
the basis for formulation of aquaculture development project and policy. Moreover, the findings
from the proposed study are important to the stakeholders and farmers for making proper
decision with regards to investment in various sub-sectors of aquaculture.
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Anticipated Benefits:
The beneficiaries of this study will be various stakeholders involved in aquaculture, including
fish farmers, processors, traders, consumers and policy markers. The expected outputs are as
follows:-
1. Main actors and their functions and areas of improvement in the Nile tilapia and African
catfish identified
2. Information on profitability of the different segments in the value chain of Nile tilapia and
African catfish made available
3. Constraints and opportunities for the different segments in the value chain of Nile tilapia and
African catfish identified
4. Solutions to overcome the barriers currently facing the various actors and to sustain and
improve market participation by small-scale fish farmers proposed.
5. Women’s and men’s roles as well as differences in the constraints and opportunities for
women and men identified and strategies to increase women participation in the different
segments in the value chain of Nile tilapia and African catfish proposed.
6. Six fish farmers association formed and registered at district level.
Research Design or Activity Plan
The study will use purposive sampling to select six districts in the country where Nile tilapia and
African catfish farming is practiced. Four villages per district will be randomly selected from the
list of villages in which fish farming is practiced. The population for the research will be smallscale
tilapia and catfish farmers, processors, traders and consumers in the selected villages. In
each village a list of households involved in fish farming will be obtained from the village
government and village extension officers. Ten small-scale fish farmers, five fish processors,
five fish traders and 10 fish consumers per village will be randomly selected to participate in the
study. Hence, the total sample size will be 240 fish farmers, 120 processors, 120 traders and 240
consumers. This study will be conducted in three (out of seven) agro-ecological zones (i.e.
southern highlands, northern highlands and coastal zones) in Tanzania. In each zone two districts
in which fish farming is predominantly practiced will be purposely selected. In each district four
villages will be selected based on the intensity of aquaculture activities. Hence, the study will be
conducted in 24 villages located in three agro-ecological zones of the country.
Methodology
Value chain analysis of farmed Nile Tilapia and African catfish
A value chain analysis (VCA) will be carried out to identify all actors involved in the chain of
farmed Nile tilapia and African catfish, the linkages between them, and the activities within each
link. Focus group discussions with key informants (i.e. fish farmers, extension officers, traders,
retailers and restaurant owners) will be performed to collect information on key players in the
value chain of Nile tilapia and their roles, how do tilapia and catfish products, information and
money flow through the supply chain, type of activities and services carried out at each level in
the supply chain and the factors that influence the performance in the value chain. Information
will also be collected on market demand, buyer requirements, quality standards, and local,
regional and national governance structures influencing the actors in the value chain. A
household survey will be carried out to collect information on household characteristics and
main economic activities. Apart from socio-economic characteristics information to be collected
from fish farmers will include fish farming practices, species cultured, quantity produced,
available markets and price and institutional supports offered to fish farmers. Information from
12

processors will include source of fish, means of fish handling and processing, added values due
to processing, transport and transportation facilities to markets and costs and revenues for their
products and problems encountered. Traders are expected to give information on source and
type of fish sold, trading facilities and information, available markets and costs and revenues of
their products. Consumers will be asked to provide information on their species and product
preferences and product standards required. In addition to value chain analysis, the study will
assess differences in women’s and men’s roles along the value chain..
Analyzing marketing margins of the different sub-sectors of Nile Tilapia and African
catfish value-chain
A household survey will be carried out to collected marketing financial data from each of the key
value chain actors i.e. producers, processors and retailers). For the fish farmers, marketing costs
are the production costs which include costs of feeds, fertilizers, veterinary drugs, labour
(family/hired), repair and maintenance of ponds. For the processors marketing costs are the
transportation, primary processing and packaging costs. For traders the marketing costs are the
transportation and all secondary processing and packaging costs. For the retailers who sell to end
consumers the marketing costs are the transportation and advertisement costs and costs related to
retail space/room utilisation.
Training and formation of fish farmers’ groups
Following the preliminary survey a training workshop on improved fish farming and best
management practices in aquaculture will be organized by the research team in cooperation with
the District Fisheries offices. In each district, the workshop will involve the fish farmers who
participated in the survey, village leaders, village extension workers and other key informants
from the targeted villages. The farmers will receive training on (i) pond construction (ii) pond
fertilization (iv) Fish feeds and feeding (v) fingerling production (vi) fish harvesting (vii) record
keeping and (viii) entrepreneurship and running of associations. The training will be conducted
at times and locations convenient for villagers participation. The training workshop will be
conducted for three days in each village. At the end of the workshop, the farmers in each village
will be asked to form fish farmers’ association, which will be responsible for input acquisition
and marketing of fish products as well as overseeing the development of the project. The project
anticipates having 40% women’s participation in the farmers association as well as in beneficiary
training and capacity building activities. The project will assist the farmers to register their
association.
a. Schedule of activities (1 July 2013 – 30 September 2015)
Activities
Q1
2013/2014
Q2 Q3 Q4 Q1
2014/2015
Q2 Q3 Q4
Objective 1:
Activity 1.1: Development and pre-testing of
questionnaire for household survey and
checklist for focus group discussion
Activity 1.2: Data collection through a
household survey
Activity 1.3: Data collection through focus
group discussion
13

Activities 2013/2014 2014/2015
Activity 1.4: Data analysis and report
writing
Objective 2:
Activity 2.1: Development and pre-testing of
questionnaire
Activity 2.2: Data collection through a
household survey
Activity 2.3: Data analysis and report
writing
Objective 3:
Activity 3.1: Development and pre-testing of
questionnaire
Activity 3.2: Data collection through a
household survey
Activity 3.3: Data analysis and report
writing
Objective 4:
Activity 4.1: Training of fish farmers
Activity 4.2: Sensitization of farmers to form
associations
Activity 4.3: Formation of fish farmers
groups
Activity 6.4: Registering fish farmers groups
at district level
FINAL REPORT WRITING
Literature cited
Berg, M., Boomsma, M., Cucco, I., Janssen, N. (2008). Making Value Chain Work better for the
poor. A toolbook for practitionners of value Chain Analysis. DFID, UK. Version 2.
Hempel, E. (2010). Value Chain Analysis in the Fisheries sector in Africa.
http://www.fao.org/fileadmin/user_upload/fisheries/docs/Value_Chain_Analysis_Report_FI
NAL_hempel.doc. Downloaded on 10 th April 2012.
Hussain M.G., Kohinoor A.H.M., Islam M.S., Mahata S.C., Ali M.Z., Tanu M.B., Hossain M.A.
and Mazid M.A. (2000). Genetic evaluation of GIFT and existing strains of Nile Tilapia,
Oreochromis niloticus L.,under on-station and on-farm conditions in Bangladesh. Asian
Fisheries Science 13: 117-126
Neves P. R., Ribeiro R. P., Vargas L., Natali M.R.M., Maehana K. R. and Marengoni N.G.
(2008). Evaluation of the Performance of Two Strains of Nile Tilapia (Oreochromis
Niloticus) In Mixed Raising Systems. Brazilian Archives of Biology and Technology 51 (3):
531 – 538
URT. (1997). National Fisheries sector policy and strategy statement. Ministry of Natural
resources and Tourism, Dar es Salaam, Tanzania.
14

Investigation 4
Title: Spat collection and nursery methods for shellfish culture by women.
Type of investigation: Experiment
Topic area: Human Nutrition and Human Health Impacts of Aquaculture (HHI)
INVESTIGATORS AND INSTITUTIONS:
US co-PI: Maria Haws, Pacific Aquaculture and Coastal Resources Center, University of
Hawaii-Hilo
Host Country PI: Narriman Jiddawi, Institute of Marine Sciences (IMS), University of Dar es
Salaam, Zanzibar, Tanzania
Host Country Co-PI: Julius Francis, Western Indian Ocean Marine Sciences Association
(WIOMSA), Zanzibar, Tanzania
Objectives:
This work builds on eight years of efforts to develop a small-scale shellfish culture industry in
Zanzibar to increase food security and family income with women being the primary
participants. Specifically, this work will address one of the primary obstacles to further
development of the small shellfish farms-how to obtain stock in a sustainable manner for the
farms. Spat collection is one of the most sustainable and cost-effective methods to obtain stock
for shellfish farms, hence methods will be tested to determine the best materials and timing for
spat collectors, and test nursery methods to rear the collected spat. Women will also be provided
training in other shellfish farming methods beyond the nursery stage.
Significance:
Women have been leaders in coastal aquaculture in East Africa being the first to culture
seaweed, which has become the major type of coastal aquaculture. The shellfish farming
development efforts started eight years ago were modeled on the seaweed farming efforts, both
of which were supported by the research and extension efforts of IMS and WIOMSA. Shellfish
farming was a nature activity for women to adopt since they were already familiar with many
bivalve species due to their reef-gleaning activities. Initially women began shellfish farming in a
low intensity fashion by placing smaller specimens of the bivalves they collected in small,
fenced-in enclosures in the intertidal area to allow these to grow to eating size. The purpose of
the “fences”, made of short stakes, was not primarily to contain the bivalves, but rather to
designate the area claimed by the woman farmer and to prevent intertidal users from treading on
the enclosed bivalves. Subsequently they found that the stakes provided good substrates for spat
collection, as high numbers of spat naturally attached to the stakes. These specimens were added
to the “farmed” bivalves in the enclosed area. Women typically gather, consume and sale nearly
every bivalve species found on the intertidal flats (~15 common species), including two species
of pearl oysters, Pinctada margaritifera and Pteria penguin (Jiddawi 2008). The latter two
15

species have been utilized for half-pearl production on a limited basis on Zanzibar and Pemba
Islands. Hence, unlike single species industries which target specific species for spat collection
and hence encounter issues with high rates of collection of undesirable species, shellfish farmers
in Zanzibar have the luxury of being able to utilize nearly all bivalve species collected on any
spat collector. Of course, developing methods which would optimize collection of the higher
value species such as oysters and pearl oysters would be most advantageous.
Collection directly from the reef and intertidal areas and grow-out of small specimens is not
necessarily the most sustainable method of obtaining stock for shellfish farms. Moreover, it does
not allow for scaling-up farms. The women shellfish farmers in Zanzibar are at the point where
scaling up would be possible if greater numbers of juvenile bivalves were available. Previous
pilot spat collection studies helped identify several areas where spat fall may be high enough to
support shellfish farms. This work will build on the preliminary efforts to conduct a one-year
trial to confirm that these locations are adequate in terms of spat settlement rates and to elucidate
annual patterns of spat settlement.
Quantified Anticipated Benefits:
Quantifiable benefits will include: amount of spat collected, number of students and technicians
trained, development of feasible spat collection methods, increased availability of information
and increased interest in culture of native species.
Metrics:
Number of institutions directly or indirectly benefiting from the training: 6
Number of individual participants in extension and technical training: estimated at 60
Number of communities benefiting from training: 8
Number of private businesses (including cooperatives and women’s groups) benefiting from
improved extension services: 10
Students involved: 2
Training modules produced: 1
CRSP newsletter articles: 1
Peer-reviewed journal article: 1
Activity Plan
Pilot studies in 2009-2010 assisted in locating several sites near the villages of Bwelo and
Nyamanzi on the Fumba Pennisula of Zanzibar where spat settlement rates on artificial collectors
was relatively high. Bewelo and Nyamanzi were among eight villages participating in previous
bivalve and pearl culture development work and their residents were among the most active in
the participatory research. Approximately 200 women on the Fumba Pennisula have participated
or benefitted from past aquaculture development efforts. Similar experiments were also conduct
near Tanga on the Eastern Coast of Tanzania and showed promising results.
16

Spat collection experiments will be conducted by establishing submerged longlines in two areas
and deploying 50 spat collectors every other month over a one year period. Three different spat
collection materials will also be tested. This will allow researchers to determine the best time of
year to deploy collectors and which material results in the highest level of spat settlement. The
latter is more complex than it may appear as spat collection for pearl oysters in the Pacific
suggest that while many materials appear to be suitable in terms of the initial spat collection rate,
some types may result in juveniles detaching themselves or being more vulnerable to predation.
Collectors will be inspected two months after deployment to obtain an estimate of the number of
juvenile bivalves which have attached and to identify these by species. Four months after
deployment, the collectors will be removed from the water and all adhering bivalves counted and
identified. The juveniles produced in this manner will be used in the nursery experiments. Two
types of cages will be tested to determine which results in the highest survival and growth of
juvenile bivalves. Data on water quality (temperature, salinity, turbidity) will be collected at each
experimental site.
Women from the two communities will participate in all aspects of this work and will be trained
in the technical details of spat collection and nursery rearing.
Statistical Analysis: Analyses will be performed using the Statistical Package for the Social
Sciences Version 10.1 (SPSS 10.1). Data on spat collection rates and survival will be tested by
month and by the type of the collection material used. Data from the nursery trial will be tested
to determine which cage type may result in higher survival and growth. In all cases, significant
results will be followed by a comparison of means using the Least Significant Difference (LSD)
Test. Normality and homogeneity of variance tests will be performed on raw data. Sample
distributions violating assumptions will be log-transformed before analysis. Data, expressed as
percentages, will be arc sine-transformed before analysis. All differences will be regarded as
significant at P < 0.05.
Schedule: work will start in July 2013 with establishment of the long-lines and procurement of
other materials. Spat collectors will be deployed in August 2013 and continued through July
2014. Nursery trials will begin four months after the first juveniles are removed from the
collectors; this is expected to occur in December 2013 or January 2014.
Student involvement: Two graduate students will be involved in this work.
Literature Cited
Crawford, B.C., M.D. Herrera, N. Hernandez, C. Rivas-LeClair, N. Jiddawi, M. Semba, M.C.
Haws. 2010. Small scale fisheries management: lessons from cockle harvesters in
Nicaragua and Tanzania. Coastal Management Journal. 38:195-215.
17

Ellis, S. and M. Haws. 1999. Producing pearls using the black-lip pearl oyster (Pinctada
margaritifera). Center for Tropical and Subtropical Aquaculture (CTSA). Honolulu, HI.
Haws, M.C., B. Crawford, S.C. Ellis, N. Jiddawi, A. Mmochi, E. Gaxiola-Camacho, G.
Rodriguez-Dominguez, G. Rodriguez, J. Francis, C. Rivas-LeClair, A. Saborio-Coze, N.
Hernandez, E. Sandoval, K. Dabrowski, M.C. Portella and M. Jaroszewska. 2010.
Aquaculture research and development as an entry-point and contributor to natural
resources and coastal management. Coastal Management Journal: 38: 238-261.
Jiddawi, N. 2008. Pearl farming in Zanzibar. SPC Pearl Farming Bulletin. May 2008. Secretariat
of the Pacific Community. Accessed at:
http://www.spc.int/DigitalLibrary/Doc/FAME/InfoBull/POIB/18/POIB18_18_Jiddawy.pdf
Jiddawi, N.S. 2012. The artisanal fisheries and other marine resources in Chwaka Bay. In:
People, Nature and Research in Chwaka Bay, Zanzibar, Tanzania. de La Torre-Castro and
Lyimo, T.J. (eds.). page 213-212. WIOMSA. Zanzibar Town, Tanzania.
Msuya, F.E., M.S. Shalli, K. Sullivan, B. Crawford, J. Tobey and A.J. Mmochi. 2007. A
Comparative Economic Analysis of Two Seaweed Farming Methods in Tanzania. Coastal
Resources Center, University of Rhode Island for USAID. Accessed at:
http://pdf.usaid.gov/pdf_docs/PNADK663.pdf
18

Investigation 5
Title: Coastal women’s shellfish aquaculture development workshop.
Type of investigation: Activity
Topic area: Human Nutrition and Human Health Impacts of Aquaculture (HHI)
INVESTIGATORS AND INSTITUTIONS:
US co-PI: Maria Haws, Pacific Aquaculture and Coastal Resources Center, University of
Hawaii-Hilo
Host Country PI: Narriman Jiddawi, Institute of Marine Sciences (IMS), University of Dar es
Salaam, Zanzibar, Tanzania
Host Country Co-PI: Julius Francis, Western Indian Ocean Marine Sciences Association
(WIOMSA), Zanzibar, Tanzania
Objectives: This workshop will bring together participants from coastal villages and technical
assistance providers together to: 1) provide training in bivalve shellfish culture methods; 2) share
results of the participatory research conducted as part of Investigation 1 and previous research;
and 3) develop an action plan for further development of the small-scale bivalve industry. The
majority of the participants in this workshop will be women and girls since they are the primary
participants in shellfish farming.
Significance: This workshop will provide an opportunity to provide training and a venue to
discuss research and training efforts provided as of the date of the workshop. Moreover, it will
bring stakeholders together to develop an action plan to guide future efforts.
Quantified Anticipated Benefits:
Anticipated benefits include: training in shellfish culture methods, development of new
partnerships and agreements and provide a venue for stakeholder participation and input.
Target groups for this work include: Women living in eight coastal villages on the Fumba
Pennisula of Zanzibar. It should be noted that this will also serve as a model effort for other areas
of Zanzibar, mainland Tanzania, Pemba Island (site of a WWF-sponsored pearl culture project)
and other areas of East Africa.
Quantifiable benefits will include:
Metrics:
Workshop participants will include community members (25), government agency
representatives (6), village heads (2), district fisheries officers (2), private sector representatives
(2), journalists (2), local NGO’s (2).
19

The findings and action plan from the workshop will also be distributed regionally through the
Western Indian Ocean Region through WIOMSA’s website (www.wiomsa.org). WIOMSA
provides services to eight member countries in the region and reaches hundreds of regular
website visitors on an international basis. The WIOMSA site has several venues to reach
stakeholders including a blog, mariculture bulletin board and Face Book site.
Activity Plan: the workshop will be primarily focused on women shellfish farmers and technical
assistance providers. A two day workshop will be held during which the following topics will be
addressed: 1) provide training in bivalve shellfish culture methods; 2) share results of the
participatory research conducted as part of Investigation 1 and previous research; and 3) develop
an action plan for further development of the small-scale bivalve industry. Furthermore, since
climate change impacts have already affected the other primary mariculture activity in East
Africa (seaweed farming), a preliminary plan for adaptation to climate change impacts will be
included on the workshop agenda.
Schedule: the workshop will be held in Year 2, most likely in May or June 2014.
Student Involvement: Two students will be directly involved in the planning and execution of
the workshop. Traditionally many other students from IMS attend these types of workshops.
20

Investigation #6
Title: Identifying local strains of Oreochromis niloticus that are adapted to future climate
conditions
Investigation Type: Experiment
Topic Area: Climate Change Adaptation: Indigenous Species Development
Principal Investigators
Lead PI: Emmanuel A. Frimpong, Department of Fish and Wildlife Conservation Virginia
Polytechnic Institute & State University, Blacksburg, VA
HC PI: Stephen Amisah, Department of Fisheries and Watershed Management, Kwame
Nkrumah University of Science & Technology, Ghana
HC co-PI: Gifty Anane-Taabeah, Department of Fisheries and Watershed Management,
Kwame Nkrumah University of Science & Technology, Ghana
Objectives
1. Conduct a comprehensive review, a meta-analysis, and synthesis of the peer-reviewed
literature on Oreochromis niloticus, with respect to various strains and their adaptive range
for temperature, dissolved oxygen (DO), and salinity.
2. Conduct laboratory experiments to test the tolerance of the Volta strain and three wild
populations of O. niloticus to increased temperature, decreased DO, and increased salinity.
3. Determine size distribution, sex ratios, and length-fecundity relationships and characterize
ambient water-quality (temperature, DO, salinity) of O. niloticus in its native habitat along
the latitudinal gradient from southern to northern Ghana in the Volta basin.
4. Genetically characterize wild populations of O. niloticus along the latitudinal gradient of the
Volta Lake.
5. Develop a predictive distribution model (i.e., zoogeography) for O. niloticus in West Africa
and accurately delineate the extremes and boundaries of the species’ native range.
Hypotheses for Experimental Studies:
Hypothesis 1: The Volta/Akosombo strain of O. niloticus grows faster compared to the average
wild strain in Ghana under current climate conditions, but the Volta strain is less tolerant of high
temperature, low dissolved oxygen, and high salinity.
Hypothesis 2: Northern populations of O. niloticus in West Africa are more tolerant of high
temperature, low dissolved oxygen, and high salinity than southern populations; and the northern
populations have a higher optimum temperature for growth.
Significance
The world’s climate is changing directionally and these changes will or are already having severe
consequences for fisheries and aquaculture and food security, especially in tropical developing
countries (Handisyde et al. 2005; Ficke et al. 2007; Leung & Bates 2013). Among impacts in
aquatic systems expected to worsen over time include increased temperature and decreased DO,
increased salinization of underground water and intrusion of salt water from sea level rise, and
increased incidence of disease outbreaks in culture systems (Handisyde et al. 2005; Ficke et al.
2007; Williams & Rota 2010; Leung & Bates 2013). Recommended solutions emphasize
adaptations and interventions that are based as much as possible on local practices and traditions,
e.g., developing tolerant strains of existing aquaculture species and enhancing the resilience of
communities, ecosystems, and traditional culture techniques (Williams & Rota 2010).
21

Nile tilapia (O. niloticus, or ‘tilapia’) is the most widely cultured species of fish in Africa
and counted on for future food security. If this species succumbs to climate change, it will be a
devastating blow to aquaculture development on the continent. Traditionally, strain selection and
breeding has targeted a few traits, primarily fast growth (e.g., GIFT and its derivatives) and other
desirable traits are secondary. But planning for climate change presents a different challenge;
cultured strains have to survive the climate and then grow. Temperature and DO tolerance have
not been primary traits for selection because the species is considered tolerant. There has been
research on hybridization of O. niloticus with its salinity-tolerant confamiliar species, e.g., O.
mossambicus and Sarotherodon galilaeus (Kamal & Mair 2005; Yan & Wang 2010) and
introduction of marine species DNA into gonads of O. niloticus (El-Zaeem et al. 2011), the goal
being to develop more strains that can survive and grow better in high salinity (El-Sayed 2006).
But introduction of genetically modified strains of O. niloticus into the native range would be
opposed because of the threat of loss of pure wild populations. As an example, the use of GIFT
strain in Africa for commercial purpose is still being debated after more than two decades of its
development. On the other hand, the selection and development of better strains locally is
encouraged. On this principle, the Volta strain of O. niloticus has been developed in Ghana and
there are wide reports of promising performance (Dewedar 2013).
Optimal temperature for survival and growth of O. niloticus has been studied under a
variety of conditions. Most studies found an optimum of 26-30
22
o C for growth, FCR and/or
survival (Likongwe et al. 1996; Al-Asgah & Ali 1997; Baras et al. 2001; Azaza et al. 2008; El-
Sayed & Kawanna 2008; Drummond et al. 2009; Xie et al. 2011). Perhaps, more intriguing is the
variation observed in the optimum and the reduced growth and increased mortality past the
optimum, well before the upper lethal temperature (Preceding references). Strain and acclimation
conditions account for some observed variation, but what has not been studied well is whether
optimum and lethal temperatures vary within the species. These studies often assume implicitly
that the physiological adaptations of the species are the same for all populations and individuals,
and that phenotypic plasticity explains observed variation in tolerances. But it is well-known in
fishes that geographic cline in traits occur. For example, the mummichog Fundulus heteroclitus
distributed along the east coast of the United States shows a latitudinal cline in temperature and
oxygen use adaptation, with underlying genotypic variation in the allelic isozymes of lactate
dehydrogenase (LDH-B) that affect ATP levels (Place 1983). Individual variation in salt
tolerance in O. niloticus has also been studied at the molecular level, although not in the context
of latitudinal clines (Rengmark et al. 2007).
Sub-Saharan Africa pond aquaculture is heavily dependent on natural ambient DO as
aeration is rare to non-existent and mostly unnecessary at moderate stocking densities. Tolerance
of O. niloticus to low DO should be understood in the context of minimum DO required for
acceptable survival, growth, and reproduction. Under recirculating conditions, O. aureus had
better FCR with intermediate (3.75±0.12 ppm), compared to low and high DOs (Papoutsoglou &
Tziha 1996). DO levels do not only influence feed intake in O. niloticus, but it also affects
growth, size at maturity, gonadosomatic index (GSI), egg size, and absolute fecundity (Kolding
et al. 2008; Tran-Duy et al. 2008). There are well known dependencies among temperature and
solubility of salt and oxygen, and plastic response of fish to one physicochemical variable also
depends on the level of other variables, in addition to interaction of genetics with environmental
factors (Charo-Karisa et al. 2006; Schofield et al. 2011). A quick search of Web of Science and
Aquatic Sciences and Fisheries Abstracts databases for the period 1970-2012 revealed more than
1,100 peer-reviewed publications on O. niloticus and and confamiliar species that focus on some
aspect of growth. However, on close examination, West Africa native strains of O. niloticus are
grossly under-represented in these studies. Most studies are from Egypt or otherwise from

outside of the continent. Identification of better-adapted populations of O. niloticus, and degree
of adaptation to temperature, DO, and salinity will require synthesis of existing knowledge on
the species, a combination of field and laboratory studies, including basic genetic descriptions,
and linkage of distribution with biophysical data.
Species exhibit their most extreme adaptations at the tail ends of their range and in
response to environmental gradients (e.g., Place 1983). In West Africa natural climate varies
from humid forest to dry savanna and desert as you move from the coast (low latitude) to interior
(high latitude). The Volta basin, which spans the entire length of Ghana into Burkina Faso, aligns
with this gradient. Reported occurrences (Paugy et al. 2003) indicate that O. niloticus range
crosses much of the climate gradient. Populations in hot, drought-prone areas are predictably
better adapted to high temperature, low DO, and high salinity. The salinity prediction is less
intuitive but not when you consider that aquatic systems subject to high evaporation tend to have
higher salinities, as drying concentrates dissolved solids. Thus, the predicted climate change
scenarios in the southern parts of Ghana where most aquaculture is concentrated is very similar
to current conditions in the north, near the upper limit of known O. niloticus West African range.
A more accurate zoogeographic model for O. niloticus would be useful as a tool to identify the
best adapted populations and also delineate the species’ range from the numerous cichlid species
(more than 40 in West Africa, Paugy et al. 2003) that are undoubtedly confused with O.
niloticus, especially by small-scale farmers who still rely significantly on wild brood and seed to
stock their ponds. Identifying these populations and their adaptations will guide future breeding
programs that will have to consider climate, and take advantage of individual variation within
populations to select for desired traits in addition to fast growth.
Anticipated Benefits
• At least one peer-reviewed manuscript synthesizing current knowledge on O. niloticus with
respect to physiological adaptive range of the species and how this information can be used
to inform climate change adaptation strategies.
• A minimum of three distinct wild populations of O. niloticus and the Akosombo strain
characterized in terms of tolerance to temperature, dissolved oxygen, and salinity.
• Length-fecundity relationships developed for of three wild populations of O. niloticus.
• A DNA library created for at least three wild populations of O. niloticus in Ghana.
• An accurate fine-grained West African distribution map of O. niloticus created to help
identify extreme and likely better-adapted populations of O. niloticus for future climate.
Study Design/Activity Plan
Location
Study will be conducted in Ghana in the Volta basin. Ghana is ideal for this study because O
niloticus is native throughout most of the nation’s geographic boundaries and span the three
biogeographic regions (forest, semi-deciduous, and savanna) over 5-6 degrees of latitude.
Methods
The following five components of the work plan follow the five objectives previously listed:
A. A comprehensive literature review and a meta-analysis and synthesis of the peer-reviewed
and other credible literature on O. niloticus performance under varying water physicochemistry
will focus on which of these factors and corresponding adaptations will have the
most significant effect on survival and growth under future climate. Data will be extracted
from published studies and standard statistical meta-analysis techniques will be employed.
23

The overarching goal will be to synthesize what we already know that is useful in guiding
climate adaptation strategies.
B. Experiments will be conducted to test the tolerance of the Volta strain and three wild
populations (tentatively ‘strains’) of O. niloticus to increased temperature, decreased DO, and
increased salinity. Fingerlings (~5g) from a trusted commercial or government hatchery
(Volta strain) and three wild populations of O. niloticus from the south humid forest, semideciduous,
and savanna (near the northern border of Ghana) bioregions. Reconnaissance
surveys of potential field sites will be conducted to verify the existence of thriving
populations of O niloticus. Wild fingerlings will come from sites established for field studies.
Fish will be reared in aquarium tanks with recirculated water at appropriate stocking
densities. Each of the three factors will be varied at two levels: The low DO will be achieved
by absence of aeration while the high (control) level will be achieved by aeration of the
source water (e.g., Tran-Duy et al. 2008). Temperature will be regulated by heater/chillers.
The control temperature will be maintained at 28
24
o C and the high at 32 o C. The control level of
salinity will be approximate 0ppm (i.e., no salt added) and the high salinity will be created by
adding appropriate quantity of sodium chloride to raise salinity to 15ppm. Dry runs will be
conducted months prior to the experiment to perfect the temperature, DO, salinity settings
within the narrow ranges desired. In addition, during the actual experiment water quality will
be monitored at the inlet at least three times a day and the levels of these three variables will
be adjusted immediately if needed. Prior to experiments all fish will be acclimated at the
control conditions. Detailed experimental design will depend on the amount of fingerling
obtainable from the field, and the degree to which reproduction timing is synchronized
among the different populations in the study region. A factorial design is desired but in the
possible event of wild populations reproducing at significantly different times a split-plot
design will be considered. Each treatment combination will have 3 replicates to result in
4(populations) X 3(factors) X 2(levels) by 3(replicates) = 72 experimental units. Experiments
will run for approximately 60-70 days, i.e., before fish reach maturity and growth will be
measured weekly on a subsample of fish. Survival will be determined by subtracting
mortalities. Fish will be fed high-quality commercial floating feed to apparent satiation and
amount of feed taken by each experimental unit will be documented to determine FCR.
C. Size distribution, sex ratios, and length-fecundity relationships of O. niloticus and ambient
water-quality (temperature, DO, salinity) in its native habitat along the latitudinal gradient
will be studied through standard field sampling using seine and other gear as locally
applicable and based on river size considerations. Water quality meters will be used to
measure physico-chemical variables in situ. Field studies will cover a period of at least 6
months with sampling once a month to capture reproductive season and to determine the best
time to collect wild fingerlings for experimental studies. Specimen for laboratory analysis
will be preserved first in 10% formalin and then in 70% ethanol and prepared for analysis
following standard ichthyologic techniques.
D. To genetically characterize populations of O. niloticus used for experimental studies and wild
populations along the latitudinal gradient of the tributaries of the Volta Lake, current PCRbased
techniques for DNA fingerprinting will be employed. Genetic samples will be taken in
the field and preserved in alcohol (i.e., no formalin fixation) to keep the integrity of samples
high. Samples will be shipped to Virginia Tech University for DNA analysis.
E. To develop a predictive distribution model for O. niloticus in West Africa and accurately
delineate the extremes and boundaries of the species’ native range, occurrence records for O.
niloticus will be obtained from published sources (e.g., Paugy et al. 2003; Fishbase
www.fishbase.org). Environmental data such as temperature, elevation, and annual rainfall

averages will be obtained from online GIS databases (e.g., www.madmappers.com). Some
environmental data, especially the more accurate and climate-relevant may be available at a
cost. Standard ‘niche’ modeling (e.g., Zambrano et al. 2006) and other improved techniques
will be employed. Such a map can also be used to examine how the natural distribution of the
species will change under future climate scenarios.
Schedule: Start date: July 1, 2013 End date: September 30, 2015
Activity/Month
J-S
2013
O-D
2013
25
J-M
2014
A-J
2014
J-S
2014
O-D
2014
J-M
2015
A-J
2015
Literature review & meta-analysis x x x x
Field Reconnaissance x
Field studies x x x x x
Laboratory analysis of field data x x x x x x
Laboratory dry runs x
Experiments x x x x
Genetic analysis x x
Acquisition of distribution data x x x
Distribution modeling x x x x
Data analysis and reporting x x x X
J-S
2015
Literature Cited
Al-Asgah, N.A., and A. Ali. 1997. Growth performance and body composition of Oreochromis
niloticus reared at different water temperatures. Annales De Zootechnie 46:331-338.
Azaza, M.S., M.N. Dhraief, et al. 2008. Effects of water temperature on growth and sex ratio of
juvenile Nile tilapia Oreochromis niloticus (Linnaeus) reared in geothermal waters in
southern Tunisia. Journal of Thermal Biology 33: 98-105.
Baras, E., B. Jacobs, et al. 2001. Effect of water temperature on survival, growth and phenotypic
sex of mixed (XX-XY) progenies of Nile tilapia Oreochromis niloticus. Aquaculture
192:187-199.
Charo-Karisa, H., H. Komen, et al. 2006. Genetic and environmental factors affecting growth of
Nile tilapia (Oreochromis niloticus) juveniles: Modelling spatial correlations between
hapas. Aquaculture 255:586-596.
Dewedar, R. 2013. Fast-growing fish variety could benefit Egypt and West Africa. The Science
and Development Network, http://www.scidev.net/en/agriculture-and-
environment/fisheries/news/fast-growing-fish-variety-could-benefit-egypt-and-westafrica-.html
(Retrieved 4/24/2013).
El-Sayed, A.-F.M. 2006. Tilapia culture in salt water: environmental requirements, nutritional
implications, and economic potentials. En: Editors Suarez, E.L.C. et al. Advances en
Nutricion Acuicola VIII. Symposium Internacional de Nutricion Acuicola. 15-17
November. Universidad Autonoma de Nuevo Leon, Monterey, Nuevo Leon, Mexico.
El-Sayed, A.-F. M., and M. Kawanna. 2008. Optimum water temperature boosts the growth
performance of Nile tilapia Oreochromis niloticus fry reared in a recycling system.
Aquaculture Research 39:670-672.
Drummond, C. D., L. D. Solis Murgas, et al. 2009. Growth and survival of tilapia Oreochromis
niloticus (Linnaeus, 1758) submitted to different temperatures during the process of sex
reversal. Ciencia E Agrotecnologia 33:895-902.

El-Zaeem, S. Y., M. M. M. Ahmed, et al. 2011. Production of salinity tolerant Nile tilapia,
Oreochromis niloticus through traditional and modern breeding methods: II. Application
of genetically modified breeding by introducing foreign DNA into fish gonads. African
Journal of Biotechnology 10:684-695.
Ficke, A.D., C.A. Myrick, et al. 2007. Potential impacts of global climate change on freshwater
fisheries. Reviews in Fish Biology and Fisheries 17:581-613.
Handisyde, N.T., L.G. Ross, et al. 2005. The effects of climate change on world aquaculture: A
global perspective. DFID, London, UK.
Kamal, H.A.M.M., and G.C. Mair. 2005. Salinity tolerance in superior genotypes of tilapia,
Oreochromis niloticus, Oreochromis mossambicus and their hybrids. Aquaculture
247:189-201.
Kolding, J., L. Haug, et al. 2008. Effect of ambient oxygen on growth and reproduction in Nile
tilapia Oreochromis niloticus. Canadian Journal of Fisheries and Aquatic Sciences
65:1413-1424.
Leung, T.L.F., and A.E. Bates. 2013. More rapid and severe disease outbreaks for aquaculture at
the tropics: implications for food security. Journal of Applied Ecology 50:215-222.
Likongwe, J. S., T. D. Stecko, et al. 1996. Combined effects of water temperature and salinity on
growth and feed utilization of juvenile Nile tilapia Oreochromis niloticus (Linneaus).
Aquaculture 146: 37-46.
Pandit, N.P., and M. Nakamura. 2010. Effect of high temperature on survival, growth, and feed
conversion ratio of Nile tilapia, Oreochromis niloticus. Our Nature 8:219-224.
Papoutsoglou, S. E. and G. Tziha. 1996. Blue tilapia Oreochromis aureus growth rate in relation
to dissolved oxygen concentration under recirculated water conditions. Aquacultural
Engineering 15:181-192.
Place, A.R. 1983. Isozymes. Current Topics in Biological and Medical Research 10(Genetics and
Evolution):147-170.
Paugy, D., C. Leveque, and G. G. Teugels. 2003. The fresh and brackish water fishes of West
Africa, Volume II. IRD Editions, Publications Scientifiques du Museum, MRAC.,
Belgium.
Rengmark, A. H., A. Slettan, et al. 2007. Identification and mapping of genes associated with
salt tolerance in tilapia. Journal of Fish Biology 71:409-422.
Schofield, P. J., M. S. Peterson, et al. 2011. Survival, growth and reproduction of non-indigenous
Nile tilapia, Oreochromis niloticus (Linnaeus 1758). I. Physiological capabilities in
various temperatures and salinities. Marine and Freshwater Research 62:439-449.
Tran-Duy, A., J. W. Schrama, et al. 2008. Effects of oxygen concentration and body weight on
maximum feed intake, growth and hematological parameters of Nile tilapia, Oreochromis
niloticus. Aquaculture 275:152-162.
Williams, L., and A. Rota. 2010. Impact of climate change on fisheries and aquaculture in the
developing world and opportunities for adaptation. International Fund for Agricultural
Development, Rome, Italy.
Xie, S., K. Zheng, et al. 2011. Effect of water temperature on energy budget of Nile tilapia,
Oreochromis niloticus. Aquaculture Nutrition 17:E683-E690.
Yan, B. A. and Z. H. Wang (2010). Growth, salinity tolerance and microsatellite analysis of the
F-2 reciprocal hybrids of Oreochromis niloticus x Sarotherodon galilaeus at different
salinities. Aquaculture Research 41:e336-e344.
Zambrano, L., E. Martinez-Meyer, et al. 2006. Invasive potential of common carp Cyprinus
carpio and Nile tilapia Oreochromis niloticus in American freshwater systems. Canadian
Journal of Fisheries and Aquatic Sciences 63:1903-1910.
26

Investigation #7
Title: Assessing Ghana’s national aquaculture training needs and capabilities and
climate change preparedness in the light of the national aquaculture development
plan
Investigation Type: Study
Topic Area: Policy Development
Principal Investigators
Lead PI: Emmanuel A. Frimpong, Department of Fish and Wildlife Conservation Virginia
Polytechnic Institute & State University, Blacksburg, VA
HC PI: Stephen Amisah, Department of Fisheries and Watershed Management, Kwame
Nkrumah University of Science & Technology, Ghana
HC co-PI: Gifty Anane-Taabeah, Department of Fisheries and Watershed Management,
Kwame Nkrumah University of Science & Technology, Ghana
Objectives
1. Assess aquaculture training needs and capabilities of Ghana against the aquaculture
growth targets set in the Ghana National Aquaculture Development Plan (GNADP)
2. Monitor and evaluate the new Kwame Nkrumah University of Science and Technology
(KNUST) BSc. Aquaculture and Water Resources program
3. Assess the climate change preparedness of Ghana’s aquaculture sector based on the
GNADP
4. Generate baseline information for monitoring and evaluation of the GNADP and the
KNUST Aquaculture and Water Resources program.
Significance
With the preponderance of evidence that the global climate is changing, it could safely be
asserted that anything that is done in management, planning, or policy that assumes a static
climate is unlikely to succeed. Unfortunately, many developing countries have not kept pace
with the need to evolve existing policies or develop new policies to adapt to change. Agriculture
has only become a significant part of the climate change policy discourse in Ghana in the last 2-3
years (Sarpong & Anyidoho 2012). The aquaculture and fisheries sector in Ghana, in spite of its
enormous potential, currently contributes only about 10% of the 30% of GDP contributed by
agriculture. Thus the aquaculture sector, itself currently a small part of fish production in Ghana,
is proportionally less of a focus in country-level policies and planning toward climate change
adaptation.
In the absence of reliable local needs assessments and articulated national strategies and
dedicated resources for aquaculture development in sub-Saharan African countries, research in
these countries tended to be donor-driven (Egna et al. 1997). Sarpong and Anyidoho (2012) note
a variety of donor-sponsored projects in Ghana on climate change since the mid-1990s but
focused almost exclusively on the energy, forestry, and the environment sectors. Aquaculture in
Ghana has enjoyed significant support from the USAID through the ACRSP and AquaFish
CRSP programs in the past 8 years or so, but along the lines of most donor-funded projects it has
been a continuing struggle to reconcile USAID objectives with national priorities and collaborate
27

with the Ministry; this made even more difficult as the Fisheries Commission underwent many
erratic structural and name changes during the period. The ACRSP/AquaFish CRSP research and
development activities in Ghana has not previously focused on climate change, but the overall
research portfolio has made significant inroads in contributing to development, notably in
capacity building. The KNUST department of Fisheries and Watershed Management which has
been the primary host-country collaborating institution in Ghana has seen the most direct benefit
of ACRSP/AquaFish capacity building, which has culminated in a new BSc. degree program in
Aquaculture and Water Resources, started in September 2012. The regional offices of the
Fisheries Commission have also benefitted from training and improvements in extension, as are
almost 500 farmers who have been trained through the ACRSP/AquaFish programs.
In March 2012, a long awaited Ghana National Aquaculture Development Plan (GNADP; FC
2012) was unveiled by the FAO and the Ministry of Food and Agriculture. The GNADP sets out
strategies to provide the capacity for fish farmers to increase production from the current
20,000MT/year to 100,000MT/year by 2016 and, thereby, increase the market share of
commercially farmed fish from $28,440,000/year to $362,000,000/year. The clock has been
ticking since unveiling this document toward the realization of these arguably lofty goals;
however change, if any has occurred, is difficult to see at this point.
A number of assumptions are embedded in the projected growth in production. A lot is expected
of local institutions such as KNUST in the area of education and training, extension, research,
and partnerships (GNADP sections 2.3-2.6). Climate change is mentioned only once as an
environmental and social concern: “Government would research on climate change and its
effects on aquaculture” (GNADP section 2.9). Neither was there a climate change research
agenda or was the plan developed with a major expectation that the climate would be changing
through the implementation of the plan and beyond. In fact, in a recent World Fish Center-led
scenario development workshop for the fisheries and aquaculture sector in Ghana, Senegal, and
Mauritius, one of the poignant participant feedbacks was a lack of representation of sector
personnel from the governments of these countries (Badjeck et al. 2010).
The need here is to capture the contribution of AquaFish CRSP to aquaculture development in
Ghana while engaging and sensitizing the sector actors to climate issues and helping shape the
agenda for a need-driven research responsive to climate change. In particular, this investigation
is designed to contribute to both formative (process) and summative (outcome) evaluations
(Kahan 2008; Kumar & Eriksson 2011) of the AquaFish programs in Ghana. The priority is the
KNUST BSc. Aquaculture and Water Resources curriculum, and its potential to contribute to
achieving the goals of the. Another question of interest is the responsiveness of the GNADP to
climate change.
Anticipated Benefits
• A clear and accessible policy-relevant document on aquaculture training needs at all levels
and the contribution of AquaFish to aquaculture development in Ghana.
• Informed and targeted recommendations to policy makers and the private sector on
opportunities for aquaculture adaptation to climate change in Ghana.
• A review and recommendations on the KNUST BSc. Aquaculture and Water Resources
curriculum and how KNUST could address some of the training needs in the sector.
• Baseline data on development targets set in the GNADP
28

• At least 30 fisheries officers trained to improve extension skills and awareness of climate
change and aquaculture development issues.
Study Design/Activity Plan
Location
Study will be conducted in Ghana with active participation of KNUST and the Ministry of
Fisheries and Aquaculture Development.
Methods
This study will adopt the outcome mapping framework of evaluation (Earl et al. 2001; Nyangaga
& Schaeffer 2011), wherein the emphasis of evaluation is on changes in behavior, relationships,
actions, and activities in the people (e.g., scientists, students, farmers, government officials),
groups (e.g., private sector actors in the aquaculture value-chain), and organizations (fisheries
departments, universities) that AquaFish works with directly. These people, groups, and
organizations are called boundary partners, representing the groups with whom our program
anticipates opportunities for influence (Earl et al. 2001). This methodology will enable AquaFish
to specifically articulate who we are targeting, what changes we expect to see, how we will
accomplish those changes, and how we measure the change. The approach minimizes the need to
deal with the complexities of attempting to quantify unique over all contributions of AquaFish to
development of the sector, where a multitude of development projects are also active and overall
impacts of any one organization is confounded. The methods will enable us to link our actions
with desired outcomes without necessarily assuming cause-effect, or with no need to claim full
credit for success.
Outcome mapping uses facilitated, participatory workshops involving as much as possible the
full range of boundary partners where there is opportunity for learning and reflection as part of
the evaluation process. The workshop approach (Earl et al. 2001) is adaptable to the Ghana
situation and serves the additional purposes of ongoing interaction with Ghana stakeholders
(boundary partners) which have proved particularly effective in both outreach and data collection
(e.g., questionnaire administration) in ongoing AquaFish work in Ghana. Worksheets will be
adapted from Earl et al. (2001) and used in a series of three three-day workshops to be held over
a period of 2 years. Consultation with stakeholders and desk studies will take place during the
months between these workshops. Baseline data will be gathered through these consultations and
desk studies. The 3 stages (Intentional Design, Outcome and Performance Monitoring, &
Evaluation Planning) and 12 steps in outcome mapping as treated by Earl et al. (2001) will be
followed. A sample output from the first workshop focused on intentional design is shown in an
appendix at the end of this investigation narrative (Appendix 1). The data generated will include
both quantitative and qualitative types and will be submitted to standard statistical analysis for
reporting and learning.
Schedule: Start date: July 1, 2013 End date: September 30, 2015
Activity/Month
J-S
2013
O-D
2013
29
J-M
2014
A-J
2014
J-S
2014
O-D
2014
Workshop 1 x
Workshop 2 x
Workshop 3 x
Consultations & Desk studies x x x x
J-M
2015
A-J
2015
J-S
2015

Data Analysis and Reporting x x x
Literature cited
Badjeck M-C., R.E. Katikiro, et al. 2011. Envisioning 2050: Climate change, aquaculture and
fisheries in West Africa. Dakar, Senegal 14-16 th April 2010. Workshop Report No. 2011-
09. World Fish/ZMT, Penang/Bremen.
Earl, F., F. Carden, et al. 2001. Outcome mapping: building learning and reflection into
development programs. International Development Research Center, Ottawa, Canada.
Egna, H.S., C.E. Boyd, et al. 1997. Introduction. Pages 1-18 in H.S. Egna and C.E. Boyd,
editors. Dynamics of Pond Aquaculture. CRC Press, Boca Raton.
Fisheries Commission (FC). 2012. Ghana National Aquaculture Development Plan (GNADP).
Ministry of Food and Agriculture, Accra, Ghana.
Kahan, B. 2008. Excerpts from review of evaluation frameworks. Saskatchewan Ministry of
Education, Saskatchewan, Canada.
Kumar, K., and J. Eriksson. 2011. A meta-evaluation of foreign assistance evaluations. Office of
the Director of US Foreign Assistance, Department of State, Washington, DC.
Nyangaga, J. and H. Schaeffer. 2011. A complementary approach to developing progress
markers. Outcome Mapping Learning Community, Ottawa, Canada.
Sarpong, D.B., and N.A. Anyidoho. 2012. Climate change and agricultural policy processes in
Ghana. Working Paper 045, Future Agricultures, Accra, Ghana.
Appendix 1: Sample Outcome Mapping design workshop output (from Earl et al. 2001)
30

Investigation #8
Proposal Title: Enhancing the nutritional value of tilapia for human health
Investigation Type: Study/Experiment
Topic Area: Sustainable feed technology and nutrient input systems
b. Principal Investigators
US PI: Rebecca Lochmann, University of Arkansas at Pine Bluff
HC-PI: Nelson W. Agbo, Department of Fisheries & Watershed Management Kwame
Nkrumah University of Science & Technology, Kumasi, Ghana
HC-coPI: Regina Edziyie, Department of Fisheries & Watershed Management Kwame
Nkrumah University of Science & Technology, Kumasi, Ghana
c. Objectives
1. Assess the availability and distribution of potential fish feed ingredients containing n-3 fatty
acids for use in Nile tilapia diets in Ghana.
2. Determine the proximate composition and fatty acid composition of potential feed
ingredients, and recommend specific inclusion rates for diets to be tested in feeding trials
with Nile tilapia in Ghana.
3. Analyze the proximate and fatty acid composition of experimental diets and fish from
feeding trials in Ghana.
4. Calculate the cost-of-gain of diets fed to fish in feeding trials in Ghana.
d. Significance
Fish production in Ghana is intensifying, necessitating the development of
appropriate complete and supplementary diets for use in semi-intensive and intensive Nile tilapia
production. The use of fish meal at high levels in fish feeds is not feasible in Ghana because of
its high price and limited supply. In contrast, a large number of oilseed and cereal by-products
are available. However, information on alternative sources of ingredients for fish feeds is limited,
and only a few investigations have been made (Nelson and Wallace, 1998; Hecht, 2007). In
concert with the effort to develop diets for tilapia, it is important to consider product quality in
terms of the nutritional content of the fish that will be produced, and the effect of these nutrients
on the health of consumers.
The suitability of feed ingredients for commercial production of both fish and feed are based on
nutrient content, economics and availability (Lochmann and Phillips 1995). The influence of
dietary ingredients on product quality is also important, and the lipid composition of the fish is
relatively easy to manipulate through the diet. Known human health benefits associated with n-3
highly unsaturated fatty acids (HUFAs, such as 20:5n-3 and 22:6n-3) include the reduction of
cardiovascular disease, arthritis, atherosclerosis, diabetes, and cancer (Horrocks and Yeo 1999;
Arterburn et al. 2006; Simopoulos 2008). There is increasing awareness of the health benefits of
different dietary lipids in the US (Wall et al. 2010), and this knowledge is spreading globally.
Both n-3 and n-6 fatty acids are essential nutrients for people, but there is a striking imbalance in
the intake of n-3 and n-6 fatty acids in the US and many developing nations. Intake of n-6 fats far
exceeds that of n-3 fats for many people, due to the widespread use of plant oils and terrestrial
31

animal fats that contain more n-6 than n-3 fatty acids (Trushenski and Lochmann 2009). Thus,
traditional diets can lead to marginal to severe deficiencies of n-3 fatty acids and a variety of
associated health problems (Connor 2000).
Fish are the most common source of n-3 HUFAs in most human diets (Tocher 2003).
Consumption of fatty fish such as salmon can prevent human deficiencies of n-3 fatty acids.
However, in most African countries the preferred fish are tilapia species. Cultured tilapia are low
in n-3 fatty acids due to minimal use of marine fish meals and oils in their diets (Trushenski et al.
2009). In the US, there are even published reports on the "harmful" effects of tilapia
consumption due to its high n-6 fatty acid content (Weaver et al. 2008) Since exclusion of
marine fish meals and oils from cultured fish diets is an environmentally sound practice,
alternative means of enhancing the n-3 content of farmed tilapia must be identified. The
challenge is to identify n-3 sources that will support fish performance and enhance the quality of
farmed tilapia for human health while maintaining production profitability. Fortunately, tilapia
can elongate and desaturate 18:3n-3 found in plant oils to form n-3 HUFAs such as 20:5n-3 and
22:6n-3, so inclusion of preformed HUFAs is not necessary for the general performance or
health of the fish (NRC 2011).
The ability to produce tilapia enriched with n-3 acids as a functional food could be a key factor
in mitigating widespread health problems associated with essential fatty acid deficiencies.
Potential feed ingredients used to enhance n-3 fatty acids in the fish must not only contain
substantial amounts of n-3 fatty acids, but also be locally available, cost-effective and palatable.
Some plant ingredients contain high concentrations of 18:3n-3 (Mckevith 2005), which can be
converted to n-3 HUFA by tilapia (Olsen et al. 1990). For instance, plants found in Ghana such
as Leucaena leucocephala and Moringa oleifera contain more than 30% 18:3n-3 in their lipids.
There is some information on the feeding value of Leucaena and Moringa in Nile tilapia
(Adeparusi and Agbede 2005; Madalla 2005), but more information is needed to optimize the
inclusion levels of these leaf meals in diets to obtain both profitable production and improved
product quality (i.e., enhanced content of n-3 fatty acids). A recent study at the University of
Arkansas at Pine Bluff (Kasiga 2012) showed that leaf meals made from these plants could be
substituted for up to 30% of the protein in soybean meal in diets of Nile tilapia without reducing
fish performance. The leaf-meal diets also significantly increased the concentration of total n-3
fatty acids and n-3 HUFAS in the fish. These preliminary results should be expanded to include
other variables. For example, the basal diet included some fish oil because the primary focus of
the study was on use of the leaf meals as protein sources. It would be preferable to test the leaf
meals in diets without any fish meal or oil, as most tilapia diets used in Ghana do not contain
these products. Also, Kasiga (2012) did not grow the fish to market size, so diets containing
these leaf meals need to be tested in fish for a full growing season, and the fatty acid composition
and taste of the market-size fish should also be assessed. In addition to the leaf meals, a survey
should also be conducted to identify any other potential feed ingredients available in Ghana that
would enhance the n-3 fatty acid content of the fish and meet the other criteria discussed
previously.
Aside from whole plant ingredients, it is possible to add isolated lipid sources to the diets also,
such as flaxseed oil. This oil contains more than 50% 18:3n-3, and does not inhibit tilapia growth
(Karapanagiotidis et al. 2007). There is a commercial producer of flaxseed oil in Cameroon,
which currently sells the oil for $350/MT. This might be close enough for Ghana to consider
using it in tilapia diets. It is also possible to maximize the retention of the desirable n-3 LC-
32

PUFAs in fish by supplying most of their dietary fat as saturated fat such as coconut oil
(Trushenski et al. 2009). This oil is widely available in tropical regions and could be used in
combination with an n-3 lipid like flaxseed oil to optimize the n-3 content of the fish while
keeping diet cost as low as possible. This strategy has not been tested in tilapia using diets
without fish meal and oil, so further verification of the strategy using diets without marine
products is needed.
e. Quantified Anticipated Benefits
The study will produce information useful for practical diet development for Nile tilapia in
Ghana that will result in production of a fish with a healthier lipid profile (enriched in n-3 fatty
acids) compared to conventionally grown fish. The study will also emphasize the importance of
using environmentally sustainable and cost-effective ingredients to accomplish the nutrient
enhancement of tilapia.
Production of healthier tilapia is part of a larger strategy to improve the nutritional status of
people in developing countries such as Ghana. The information will be summarized in lay
publications suitable for distribution at workshops or posting on websites. The information will
also be prepared for publication in peer-reviewed journals.
Quantifiable: The number of suitable ingredients identified and characterized in the survey as
having good potential to enhance n-3 fatty acids in tilapia (target = 5 minimum); number of lay
publications produced (at least one); number of journal articles produced (at least one).
f. Research Design/Activity Plan
1. Location
This study will be conducted in Ashanti and Brong Ahafo regions of Ghana and the feed trial in
the FRNR facilities in the KNUST, as described in the HC proposal. UAPB will assist in the
survey to identify new feed ingredients, and will analyze the proximate composition and fatty
acid composition of the ingredients. Following analysis, UAPB will advise HC personnel on
inclusion levels of the most promising ingredients to include in feeding trials. After diet
formulation at KNUST, UAPB will also analyze the proximate and fatty acid composition of the
finished diets. Following each feeding trial, UAPB will analyze samples of fish for proximate
and fatty acid composition.
2. Methods
Proximate analysis: Dry matter and ash will be determined by drying the sample at 135 o C for 2
h, and burning the sample at 600 o C for 3 h, respectively (AOAC 1995). Crude fiber (of feed
ingredients and diets only) will be determined according to Ankom filter bag technique (AOCS
2005; Ankom 200 fiber analyzer, Ankom Technology Corp., Fairport, New York). Crude protein
will be analyzed with the Macro-Kjeldahl method of total nitrogen analysis and the protein
content in samples determined by multiplying the nitrogen values by 6.25. Total lipid will be
determined using chloroform/methanol (Folch et al. 1957). The nitrogen free extract (NFE), a
measure of soluble carbohydrates, will be calculated by subtracting the percentage protein, lipid,
ash, fiber and moisture from 100. Lipid extracts from the diet ingredients, diets and fish will be
used for fatty acid analysis. Ten mL of the lipid extracts will be evaporated under nitrogen and
then trans-esterified with 14 % boron trifluoride. The resulting fatty acid methyl esters (FAMEs)
will be analyzed (Morrison and Smith 1964) using a flame ionization gas chromatograph with
helium as the carrier gas. The FAMEs will be separated on a fused silica capillary column (15 m
x 0.25 mm internal diameter). The injection volume will be 1 µl, with an injector and detector
33

temperature of 250°C and 315°C, respectively. The column temperature will be held initially at
100°C for 10 min, increased to 160°C at a rate of 15°C/min and held for 4 min, then increased to
250°C at a rate of 2.5°C/min. The FAMEs will be identified and quantified by comparing the
retention time and peak area to those of serially diluted mixtures of reference standards. After the
feeding trials and all analyses, the cost-of-gain will be calculated by determining the cost of the
amount of feed (kg) required to produce 1 kg of tilapia. The costs of the diets and feed
conversion data will be used to generate cost-of-gain.
g. Schedule
Start date: The study will begin July 1, 2013, and end September 30, 2015.
h. Literature Cited
Adeparusi, E. O., and J. O. Agbede. 2005. Evaluation of Leucaena and Gliricidia leaf protein
concentrate as supplements to bambara groundnut Vignas subterranean in the diet of
Oreochromis niloticus. Aquaculture Nutrition 12:335-342.
AOAC (Association of Official Analytical Chemists). 1995. Official methods of analysis.
Arlington, Virginia.
AOAC (Association of Official Analytical Chemists). 2005. Official methods of analysis.
Arlington, Virginia.
Arterburn, L.M., E.B. Hall, and H. Oken. 2006. Distribution, interconversion, and dose response
of n-3 fatty acids in humans. American Journal of Clinical Nutrition
83(Supplement):1467S-1476S.
Connor, W. E. 2000. Importance of n-3 fatty acids in health and disease. American Journal of
American Journal of Clinical Nutrition 71:171-175
Folch, J., M. Lees, and G. H. Sloane-Stanley. 1957. A simple method for the isolation and
purification of total lipids from animal tissues. Journal of Biological Chemistry 226:497-
509.
Horrocks, L.A., and Y.K. Yeo. 1999. Health benefits of docosahexaenoic acid (DHA).
Pharmacological Research 40:211-225.
Hecht, T. (2007) Review of feeds and fertilizers for sustainable aquaculture development in sub-
Saharan Africa. In: Hasan, M.R., Hecht, T., De Silva, S.S. and Tacon, A.G.J., (Eds.)
Study and analysis of feeds and fertilizers for sustainable aquaculture development, pp.
77-109. Rome: FAO.
Karapanagiotidis, I.T., Bell, M.V., Little, D.C., Yakupitiyage, A., 2007. Replacement of dietary
fish oils by alpha-linolenic acid-rich oils lowers omega-3 content in tilapia flesh. Lipids
42:547–559.
Kasiga, T. 2012. Evaluation of Moringa oleifera and Leucaena leucocephala leaf meals as
alternative protein sources in diets of Nile tilapia. M.S. Thesis. University of Arkansas at
Pine Bluff.
Madalla, N. 2008. Novel feed ingredients for Nile tilapia Oreochromis niloticus L. Doctoral
dissertation, Institute of Aquaculture, University of Stirling, Scotland.
Mckevith, B. 2005. Nutritional aspects of oil seeds. British Nutrition Foundation Bulletin 30:13-
26.
Morrison, W. R., and L. M. Smith. 1964. Preparation of fatty acid methyl esters and dimethyl
esters and dimethylacetals from lipids with boron fluoride-methanol. Journal of Lipid
Research 5:600-608.
34

Nelson, E. and Wallace, P. (1998) Report on a survey of agroindustrial byproducts in Northern
Ghana. Aquatic Research Institute Technical Report (Available from WRI, Ghana).
NRC (2011) Nutrient Requirements of Fish. National Research Council. Washington D.C.:
National Academy Press.
Simopoulos, A. 2008. The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular
disease and other chronic diseases. Experimental Biology and Medicine 233:674-688.
Tocher, D. R. 2003. Metabolism and functions of lipids and fatty acids in teleost fish. Reviews in
Fisheries Science 11:107-184.
Trushenski, J.T., J. Boesenberg, and C.C. Kohler. 2009. Influence of grow-out feed fatty acid
composition on finishing success in Nile tilapia. North American Journal of Aquaculture
71:242-251.
Trushenski, J., and R. Lochmann. 2009. Potential, implications and solutions regarding the use of
rendered animal fats in aquafeeds. American Journal of Animal and Veterinary Sciences
4(4):108-128.
Wall, R., R.P. Ross, G.F. Fitzgerald, and C. Stanton. 2010. Fatty acids from fish: the antiinflammatory
potential of long-chain omega-3 fatty acids. Nutrition Reviews 68)5):280-
289.
Weaver, K.L., P. Ivester, J.A. Chilton, M.D. Wilson, P. Pandey, and F.H. Chilton. 2008. The
content of favorable and unfavorable polyunsaturated fatty acids found in commonly
eaten fish. Journal of the American Dietetic Association 108(7):1178-1185.
35

Outreach and Dissemination Plan
Outreach and dissemination strategies will focus on enhancing human and structural capacity in
expanded markets and trade, particularly for women, expanding market opportunities through
market intelligence and information sharing; and the production of quality food to ensure longterm
sustainability of aquaculture in Ghana and Kenya. Project outreach activities will
incorporate the active engagement of stakeholders (including fish farmers, fish traders and
retailers, policy makers, NGOs, and environmental advocacy groups).
Dissemination efforts will include developing working partnerships with government
institutions, and non-governmental agencies to reach as far and wide as possible. The
development of value chain in Tanzania, for example, will involve organizing fish farmers into
"study" groups for case-study assignments with middlemen, fish traders, and other retailers.
Knowledge generated from the various investigations will be disseminated through
formal and informal presentations at the local level training programs, regional, and national
conferences and seminars, farmer's education and extension education meetings, and through
publications. Publications would include research articles in peer-reviewed journals, and
extension publications in local languages. Training in the use of a Seafood Market information
System in Ghana will enhance tilapia trade activities among stakeholders, particularly fish
farmers, fishermen, fish processors, seafood traders and retailers.
To engage policy makers, politicians will be invited to organized seminars and
workshops, and also taken on tours to project sites. To keep the USAID mission in the respective
countries properly informed, this project will adopt the Aquaculture CRSP Ambassador program.
Visiting US PIs and host country PIs will visit USAID mission offices for discussions on
potential areas of collaboration on AquaFish CRSP projects. All reports and published materials
from this project will be made available to USAID mission offices, government offices, nongovernmental
agencies, and the respective universities and agencies that have a stake in
aquaculture and natural resources. Host countries will also promote AquaFish project activities
on various campuses and highlight accomplishments to administrators.
The focus of outreach and dissemination strategies will not only be in-country, but across
the region among Ghana, Kenya, Uganda and Tanzania. Cross country visits by HC PIs will be
encouraged including arrangements of joint sponsorship of and participation in training programs
and involvement in cross-country research through research supervision. The expertise of HC PIs
will be brought into collaborative arrangements depending on the needs and objectives of each
country.
36

Gender Inclusivity Strategy
Women constitute a significant portion of people involved in fish marketing in Ghana and
Tanzania. Women play the role of middlemen, fish vendors, and restaurants owners. Men, on the
other hand constitute a greater proportion of fish farmers. The culture in Ghana and Tanzania is
that, men usually do the farming and the women do the marketing. Consequently, this proposal
seek to provide equal opportunities for women because a directed involvement of women is one
of the keys to advancing economic and social development not only in aquaculture but on a
holistic household and family economy. The intent of this project is to ensure maximum
participation of women in the training, educational activities, and opportunities proposed herein.
Female fisheries Officers, who serve as aquaculture extension agents, fish farmers, course
instructors, and students will be actively recruit to participate in this project. We have
demonstrated this inclusivity in the past through the selection of participants in the AquaFish
CRSP short courses we have offered as well as in the selection of students supported in graduate
programs. We shall not apologize for deliberately going out of our way to select clusters that
have more women representation in this project. Past experience shows that similar projects have
had more impact when women are represented and are provided with opportunities to advance
themselves.
37

Project Level Monitoring and Evaluation
38

Project Level Monitoring and Evaluation
The project aligns with AquaFish Innovation Lab commitment to sustainably reduce global
hunger and poverty. This project includes strategies for improving the livelihoods of smallholder
farmers and fishers, fish traders and retailers, as well as strategies to enhance growth in the
aquaculture sector and improve the nutritional status of food and humans. This project involves
various stakeholders but emphasis is being place on gender equality, focusing on maximum
participation of females.
An aspect of this project is the focus on utilizing local solutions to develop nutritious fish
feeds, native shellfish hatchery technologies, native fish species, and implementing efficient
value chain performance and market information sharing. The development of a Seafood Market
Information System in Ghana will help to expanded markets and trade, particularly for women,
which ultimately will help to improve incomes.
Various indicators will be utilized to monitor and assess the impact of this project. This
will follow the Feed the Future Monitoring System (FTFMS), which includes the number of
individuals who receive short-term and long-term assistance from the project the aquaculture
sector productivity and food security training; number of new technologies or management
practices under research; number of new technologies or management practices under field
testing; and number of new technologies or management practices made available for transfer
and adoption. The number of private for-profit enterprises, producers organizations, water users
associations, women`s groups, trade and business associations, and community-based
organizations that applied findings from this project will also be measured.
The target is that, by September 2015, 550 individuals who have received short-term and
long-term assistance from the project on aquaculture productivity and food security training; 6
technologies and/or management practices would have been researched and made available for
transfer and adoption; and 10 private for-profit enterprises, producers organizations, water users
associations, women`s groups, trade and business associations, and community-based
organizations would have applied findings from this project.
39

Host Country Research Location Considerations
GHANA
Ghana has moved significantly over the past two decades towards a stable democracy, with 3 to
4 major political parties. There is however the traditional system, where every village and town
has its chief and a council of elders. The 22 million inhabitants speak about 60 different
languages but the major ones are Akan, Ga, Ewe, Dagbani, Fante and Hansa. English is the
official language and is widely spoken throughout the country. Travel through Ghana is fairly
easy because of good access roads across the country. Intercity travel is easy by private
commercial transportation as well as the governmental owned transport company. Intra city
travel is easy with numerous taxis and private commercial mini buses or small trucks.
Ghana promotes international commerce and trade and has a relatively simple tariff
structure, comprising a low rate of 0-5% for primary products, capital goods, and some basic
consumer goods, 10% for raw materials and intermediate inputs, as well as some consumer
goods, and 25% for final consumer goods. However, there are a number of programs under
which imports can be exempted from import duties and manufacturers can apply for permission
to import raw materials and intermediate inputs at concessionary duty rates.
Ghana has recognized the importance of aquaculture to help offset the nation’s deficit of
about 400,000 metric tons of the country’s fish requirements. Consequently, the government is
aggressively pursuing an aquaculture development policy to make fish farming a profitable
business venture and not as a hobby. The government is adopting strategies to address
constraints such as quality fish seed and feed, inadequate extension services and support, and
marketing. The government has increased staff levels and provided training to improve expertise
and skills to assist fish farmers with extension services. Small-scale farming dominates the
industry producing various species of tilapia such as Oreochromis niloticus, Tilapia zillii,
Sarotherodon galilaeus and Hemichromis fasciatus, Heterotis niloticus and the catfishes (Clarias
gariepinus and Heterobranchus bidorsalis). The government is also actively supporting an FAO
project in the Volta Basin to develop fast-growing tilapia specie, the Genetically Improved
Farmed Tilapia (GIFT) that would make fish farming commercially and economically attractive.
GIFT takes about four to six months to mature for harvesting.
The Ministry of Fisheries (MOF) is the lead agency with administrative control of
aquaculture responsible for planning and development in the aquaculture sub-sector. The MOF
does not offer credit facilities to fish farmers but promotes the formation of fish farmers
associations (FFAs). MOF support the growth of the FFAs by providing each FFA a D6
bulldozer on 7-year credit for the construction of ponds. The FFAs are responsible for lobbying
on behalf of fish farmers, organizing purchase of inputs such as seeds and credit, and arranging
markets for farmers’ harvests. The Water Research Institute (WRI) of the Council for Scientific
and Industrial Research (CSIR) is responsible for aquaculture research in Ghana. The WRI is the
major aquaculture research institution in the country, although the universities - Renewable
Natural Resources Institute at Kwame Nkrumah University of Science and Technology
(KNUST), Department of Fisheries and Oceanography at University of Ghana (UG), and the
Department of Fisheries and Aquatic Sciences at University of Cape Coast (UCC) also conduct
some aquaculture research. These three universities and one agricultural college offer training in
aquaculture as well.
40

TANZANIA
Tanzania used to be ruled by only one party, the Chama Cha Mapinduzi (CCM) but the one party
state ended in 1992 when amendments were made to the constitution and a number of laws were
enacted to permit and regulate the formation and operations of more political parties. The
country currently has twelve registered political parties, in addition to the ruling party (CCM).
Tanzania has more than 126 tribes and each ethnic group has its own language. Swahili is the
official national language and is used for official business as well as inter-ethnic
communications. However, English is taught in schools and is used for some official businesses
and is used as a medium of instruction in the universities. Transportation network in Tanzania is
satisfactory, mainly road transport, and rail. The quality of the road network is limited with
paved highways confined to the north-eastern, central-eastern, and south-western regions of the
country, including Morogoro, the AquaFish project site. Intercity travel is by private commercial
transportation, mainly mini buses and trucks. Intra city travel occurs with taxis and private
commercial mini buses, the “matatu.”
The major health problems in Tanzania are communicable diseases, particularly HIV
AIDS and obstetric complications while malaria is the leading parasitic disease. Other major
parasitic diseases include hookworms, bilharzia, trypanosomiasis and intestinal worms. Tanzania
has some modern medical services provided by various governmental and non-governmental
institutions such as Christian missions (private nonprofit agencies), and a relatively small
number of private for profit practitioners.
The Tanzania government adopted its first market-oriented national trade policy in
February 2003 and implemented trade reforms resulting in substantial reduction in its average
tariff protection. Tanzania is a member of the Southern African Development Community
(SADC), the East African Community (EAC), the Cross Border Initiative, and is considering
reentering the Common Market for Eastern and Southern Africa (COMESA) from which it
withdrew in 2000.
Aquaculture in Tanzania is dominated by small-scale freshwater fish farming. Farming
practices include both extensive and semi-intensive fish farming but majority are extensive small
fish ponds integrated with other agricultural activities such as crop and animal production. Fish
production activities are found mainly in Ruvuma, Iringa, Mbeya and Kilimanjaro. Several
species are cultured, but mainly dominated by tilapia (Oreochromis niloticus). Other species with
potential for use in aquaculture include the milkfish (Chanos chanos), the flathead grey mullet
(Mugil cephalus), the North African catfish (Clarias gariepinus), and shellfish such as shrimp,
molluscs, crabs, oysters and mussels. Various species of seaweed is farmed in Tanzania, mainly
in coastal areas along the coastline from Tanga in the north to Mtwara in the south, and in the
islands of Mafia and Zanzibar.
The Fisheries Division is the governmental agency responsible for the formulation,
implementation, and enforcement of aquaculture policy and legislation. The Division conducts
numerous workshops to create awareness in the communities on sustainable aquaculture, and
provides low interest loans and a three-year tax-free period for investors in commercial
aquaculture through the National Investment Center (NIC). Several institutions are involved in
fisheries research, education and training. The Tanzania Fisheries Research Institute (TAFIMA)
has overall responsibility for all the research on fisheries; the Faculty of Aquatic Sciences and
Technology (FAST) at the University of Dar Es Salaam and the Sokoine University of
Agriculture (SUA) are both responsible for carrying out research and training on fisheries. Other
government Fisheries Centres and Institutes are involved in training as well.
41

Purdue Budget
Lead Principal Investigator
Budget Period: Year 1
From (mm/dd/yy):
7/1/2013
Kwamena Quagrainie
To (mm/dd/yy):
9/30/2013
Budget Categories US Cost US Cost-share HC/Regional Cost HC Matching
Personnel Costs
Contr ibution
Contr ibution
Salary $ 5,082 $ 3,123 $ 4,797
Benefits/Fringe $ 472 $ 483 $ 393
Subtotal $ 5,554 $ 3,606 $ 5,190 $ -
Supplies and Services
(Itemized S& S)
Subcontract to US Inst #1 -
$ 258
Virginia Polytechnic Institute &
State University
Subcontract to US Inst #2 -
$ 37,338 $ 5,724 $ 15,000 $ 5,830
University of Arkansas at Pine
Bluff
$ 19,993 $ 14,083 $ 9,993 $ 8,250
Subcontract to US Inst #3 -
University of Hawaii at Hilo
Subcontract to HC Inst #1 -
$ 15,332 $ 11,563 $ 4,416 $ -
Kwame Nkrumah University of
Science & Technology, Ghana
Subcontract to HC Inst #2 -
$ 15,648 $ - $ 15,648 $ 5,697
Sokoine University of
Agriculture, Tanzania
$ 5,098 $ - $ 5,098 $ 8,160
Subtotal $ 93,667 $ 31,370 $ 50,155 $ 27,937
Travel
Domestic $ 1,388
International $ 6,000
Subtotal $ 7,388
Equipment $ -
Tuition $ 2,691
BUDGET FORM
AquaFish Innovation Lab
Single-Year US Lead Partner Institution Proposal Budget
Proposal Title Institution
Aquaculture Production and Human Health, Nutrition, and
Food Supply in Ghana and Tanzania Purdue University
Total Direct Cost $ 109,300 $ 34,976 $ 55,345
Indirect Costs
Standard Indirect $ 4,752 $ 1,298
Indirect on Subcontracts $ 7,355 $ -
Subtotal $ 12,107 $ 1,298
Total $ 121,407 $ 36,274 $ 55,345 $ 27,937
30% 46% 23%
Additional US Non-Federal Cost
Share from US Inst #1 - Virginia
Polytechnic Institute & State
University
Additional US Non-Federal Cost
Share from US Inst #2 - University of
Arkansas at Pine Bluff
Additional US Non-Federal Cost
Share from US Inst #3 - University of
Hawaii at Hilo
GRAND TOTAL $ 121,407 $ 36,274 $ 55,345 $
27,937
41

Lead Principal Investigator
Budget Period: Year 2
From (mm/dd/yy):
10/1/2013
Kwamena Quagrainie
To (mm/dd/yy):
9/30/2014
Budget Categories US Cost US Cost-share HC/Regional Cost HC Matching
Personnel Costs
Contr ibution
Contr ibution
Salary $ 20,437 $ 12,565 $ 19,282
Benefits/Fringe $ 1,901 $ 1,945 $ 1,581
Subtotal $ 22,338 $ 14,510 $ 20,863 $ -
Supplies and Services
(Itemized S& S)
Subcontract to US Inst #1 -
$ 2,431
Virginia Polytechnic Institute &
State University
Subcontract to US Inst #2 -
$ 106,959 $ 20,351 $ 47,500 $ 12,265
University of Arkansas at Pine
Bluff
$ 51,838 $ 23,083 $ 31,838 $ 11,550
Subcontract to US Inst #3 -
University of Hawaii at Hilo
Subcontract to HC Inst #1 -
$ 99,776 $ 11,563 $ 60,637 $ 24,723
Kwame Nkrumah University of
Science & Technology, Ghana
Subcontract to HC Inst #2 -
$ 30,349 $ - $ 30,349 $ 10,297
Sokoine University of
Agriculture, Tanzania
$ 22,451 $ - $ 22,451 $ 8,160
Subtotal $ 313,804 $ 54,997 $ 192,775 $ 66,995
Travel
Domestic $ -
International $ 27,230
Subtotal $ 27,230
Equipment $ -
Tuition $ 10,893
BUDGET FORM
AquaFish Innovation Lab
Single-Year US Lead Partner Institution Proposal Budget
Proposal Title Institution
Aquaculture Production and Human Health, Nutrition, and
Food Supply in Ghana and Tanzania Purdue University
Total Direct Cost $ 374,265 $ 69,507 $ 213,638
Indirect Costs
Standard Indirect $ 18,720 $ 5,224
Indirect on Subcontracts $ 10,645 $ -
Subtotal $ 29,365 $ 5,224
Total $ 403,630 $ 74,731 $ 213,638 $ 66,995
19% 53% 17%
Additional US Non-Federal Cost
Share from US Inst #1 - Virginia
Polytechnic Institute & State
University
Additional US Non-Federal Cost
Share from US Inst #2 - University of
Arkansas at Pine Bluff
Additional US Non-Federal Cost
Share from US Inst #3 - University of
Hawaii at Hilo
GRAND TOTAL $ 403,630 $ 74,731 $ 213,638 $ 66,995
42

Lead Principal Investigator
Budget Period: Year 3
From (mm/dd/yy):
10/1/2014
Kwamena Quagrainie
To (mm/dd/yy):
9/30/2015
Budget Categories US Cost US Cost-share HC/Regional Cost HC Matching
Personnel Costs
Contr ibution
Contribution
Salary $ 20,850 $ 8,160 $ 19,668
Benefits/Fringe $ 1,940 $ 1,611 $ 1,613
Subtotal $ 22,790 $ 9,771 $ 21,280 $ -
Supplies and Services
(Itemized S& S)
Subcontract to US Inst #1 -
$ 3,132
Virginia Polytechnic Institute &
State University
Subcontract to US Inst #2 -
$ 93,261 $ 21,438 $ 35,500 $ 2,620
University of Arkansas at Pine
Bluff
$ 28,170 $ 8,083 $ 18,170 $ 11,550
Subcontract to US Inst #3 -
University of Hawaii at Hilo
Subcontract to HC Inst #1 -
$ - $ - $ - $ -
Kwame Nkrumah University of
Science & Technology, Ghana
Subcontract to HC Inst #2 -
$ 14,499 $ - $ 14,499 $ 10,297
Sokoine University of
Agriculture, Tanzania
$ 22,451 $ - $ 22,451 $ 8,160
Subtotal $ 161,513 $ 29,521 $ 90,620 $ 32,627
Travel
Domestic $ -
International $ 14,865
Subtotal $ 14,865
Equipment $ -
Tuition $ 11,111
BUDGET FORM
AquaFish Innovation Lab
Single-Year US Lead Partner Institution Proposal Budget
Proposal Title Institution
Aquaculture Production and Human Health, Nutrition, and
Food Supply in Ghana and Tanzania Purdue University
Total Direct Cost $ 210,279 $ 39,292 $ 111,900
Indirect Costs
Standard Indirect $ 14,684 $ 3,518
Indirect on Subcontracts $ - $ -
Subtotal $ 14,684 $ 3,518
Total $ 224,963 $ 42,810 $ 111,900 $ 32,627
19% 50% 15%
Additional US Non-Federal Cost
Share from US Inst #1 - Virginia
Polytechnic Institute & State
University
Additional US Non-Federal Cost
Share from US Inst #2 - University of
Arkansas at Pine Bluff
Additional US Non-Federal Cost
Share from US Inst #3 - University of
Hawaii at Hilo
43

Lead Principal Investigator
Budget Period: Year 3
From (mm/dd/yy):
10/1/2014
Kwamena Quagrainie
To (mm/dd/yy):
9/30/2015
Budget Categories US Cost US Cost-share HC/Regional Cost HC Matching
Personnel Costs
Contr ibution
Contribution
Salary $ 20,850 $ 8,160 $ 19,668
Benefits/Fringe $ 1,940 $ 1,611 $ 1,613
Subtotal $ 22,790 $ 9,771 $ 21,280 $ -
Supplies and Services
(Itemized S& S)
Subcontract to US Inst #1 -
$ 3,132
Virginia Polytechnic Institute &
State University
Subcontract to US Inst #2 -
$ 93,261 $ 21,438 $ 35,500 $ 2,620
University of Arkansas at Pine
Bluff
$ 28,170 $ 8,083 $ 18,170 $ 11,550
Subcontract to US Inst #3 -
University of Hawaii at Hilo
Subcontract to HC Inst #1 -
$ - $ - $ - $ -
Kwame Nkrumah University of
Science & Technology, Ghana
Subcontract to HC Inst #2 -
$ 14,499 $ - $ 14,499 $ 10,297
Sokoine University of
Agriculture, Tanzania
$ 22,451 $ - $ 22,451 $ 8,160
Subtotal $ 161,513 $ 29,521 $ 90,620 $ 32,627
Travel
Domestic $ -
International $ 14,865
Subtotal $ 14,865
Equipment $ -
Tuition $ 11,111
BUDGET FORM
AquaFish Innovation Lab
Single-Year US Lead Partner Institution Proposal Budget
Proposal Title Institution
Aquaculture Production and Human Health, Nutrition, and
Food Supply in Ghana and Tanzania Purdue University
Total Direct Cost $ 210,279 $ 39,292 $ 111,900
Indirect Costs
Standard Indirect $ 14,684 $ 3,518
Indirect on Subcontracts $ - $ -
Subtotal $ 14,684 $ 3,518
Total $ 224,963 $ 42,810 $ 111,900 $ 32,627
19% 50% 15%
Additional US Non-Federal Cost
Share from US Inst #1 - Virginia
Polytechnic Institute & State
University
Additional US Non-Federal Cost
Share from US Inst #2 - University of
Arkansas at Pine Bluff
Additional US Non-Federal Cost
Share from US Inst #3 - University of
Hawaii at Hilo
GRAND TOTAL $ 224,963 $ 42,810 $ 111,900 $
32,627
44

BUDGET FORM
AquaFish Innovation Lab
Combined-Year US Lead Partner Institution Proposal Budget
Proposal Title
Institution
Aquaculture Production and Human Health, Nutrition, and
Food Supply in Ghana and Tanzania
Purdue University
Lead Principal Investigator
Budget Period: Combined Years
From (mm/dd/yy):
7/1/2013
Kwamena Quagrainie
To (mm/dd/yy):
9/30/2015
Budget Categories US Cost US Cost-share HC/Regional Cost HC Matching
Personnel Costs
Contr ibution
Contr ibution
Salary $ 46,369 $ 23,848 $ 43,746 $ -
Benefits/Fringe $ 4,313 $ 4,039 $ 3,587 $ -
Subtotal $ 50,682 $ 27,887 $ 47,333 $ -
Supplies and Services
(Itemized S& S)
Subcontract to US Inst #1 -
$ 5,821 $ - $ - $ -
Virginia Polytechnic Institute &
State University
Subcontract to US Inst #2 -
$ 237,558 $ 47,513 $ 98,000 $ 20,715
University of Arkansas at Pine
Bluff
$ 100,001 $ 45,249 $ 60,001 $ 31,350
Subcontract to US Inst #3 -
University of Hawaii at Hilo
Subcontract to HC Inst #1 -
$ 115,108 $ 23,126 $ 65,053 $ 24,723
Kwame Nkrumah University of
Science & Technology, Ghana
Subcontract to HC Inst #2 -
$ 60,496 $ - $ 60,496 $ 26,291
Sokoine University of
Agriculture, Tanzania
$ 50,000 $ - $ 50,000 $ 24,480
Subtotal $ 568,984 $ 115,888 $ 333,550 $ 127,559
Travel
Domestic $ 1,388 $ - $ - $ -
International $ 48,095 $ - $ - $ -
Subtotal $ 49,483 $ - $ - $ -
Equipment $ - $ - $ - $ -
Tuition $ 24,695 $ - $ - $ -
Total Direct Cost $ 693,844 $ 143,775 $ 380,883 $ 127,559
Indirect Costs
Standard Indirect $ 38,156 $ 10,039 $ - $ -
Indirect on Subcontracts $ 18,000 $ - $ - $ -
Subtotal $ 56,156 $ 10,039 $ - $ -
Total $ 750,000 $ 153,814 $ 380,883 $ 127,559
21% 51% 17%
Additional US Non-Federal Cost
Share from US Inst #1 - Virginia
Polytechnic Institute & State
University
Additional US Non-Federal Cost
Share from US Inst #2 - University of
Arkansas at Pine Bluff
Additional US Non-Federal Cost
Share from US Inst #3 - University of
Hawaii at Hilo
GRAND TOTAL $ 750,000 $ 153,814 $ 380,883 $
127,559
45

Year 1 (7/1/13 - 9/30/13)
1. Personnel, Benefits, and Cost Share
BUDGET JUSTIFICATION FORM
FOR US LEAD PARTNER INSTITUTIONS - PURDUE UNIVERSITY
1a. Salary Information
Name Title Annual
Salary
Benefits Rate Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Kwamena
Quagrainie
Lead PI 94,860 27.7% 0.01 285 79 0.07 1,164 322
Graduate
Assistant
MS student 38,372 8.2% 0.5 4,796 393
Graduate
Assistant
MS student 38,372 8.2% 0.25 1,959 161
Total 5,081 472 3,123 483
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
1c. Cost Share
7% of the Lead PI’s time and 25% of one graduate student assistantship with benefits valued at $3,606 is
used as cost share for year 1.
2. Services and Supplies
Communications (phone calls, faxes, mailings) $50
Materials and supplies $100
MS student medical insurance $108
Subcontract to Virginia Polytechnic Institute & State University $37,338
Subcontract to University of Arkansas at Pine Bluff $19,993
Subcontract to University of Hawaii at Hilo $15,332
Subcontract to Kwame Nkrumah University, Ghana $15,648
Subcontract to Sokoine University of Agriculture, Tanzania $5,098
Total $93,667
3. Travel
Domestic Travel
Date July 2013 Name Kwamena Quagrainie Reason for travel: Pre-Synthesis
Workshop and Orientation Meeting
Departure: Indianapolis, IN Arrival: Washington, DC
Meals and Lodging @ $256/day for 3 days $768
Airfare $500
Miscellaneous (Ground transport, parking, etc.) $120
Sub Total $1,388
International Travel
46

Date August 2013 Name Kwamena Quagrainie Reason for travel: Africa Regional
AquaFish Innovation Lab Planning Meeting
Departure: Indianapolis, IN Arrival: Accra, Ghana
Meals and Lodging @ $ 250/day for 8 days $2,000
Airfare $3,000
Miscellaneous (Ground transport, parking, visa, etc.) $1,000
Sub Total $6,000
TOTAL TRAVEL $7,388
4. Tuition
Tuition Remission: $2,691
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
36% On-campus $4,752
Rate of Indirect on Amount per
Number of Subcontracts Total Amount
Subcontracts
Subcontract
36% $20,430 2 $7,355
6. HC/Regional Cost: $56,255, which is 51% of Total Direct Cost and 46% of Total Project Cost.
47

Year 2 (10/1/13 - 9/30/14)
1. Personnel, Benefits, and Cost Share
BUDGET JUSTIFICATION FORM
FOR US LEAD PARTNER INSTITUTIONS - PURDUE UNIVERSITY
1a. Salary Information
Name Title Annual
Salary
Benefits Rate Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Kwamena
Quagrainie
Lead PI 96,757 27.7% 0.02 1,155 320 0.07 4,688 1,299
Graduate
Assistant
MS student 38,564 8.2% 0.5 19,282 1,581
Graduate
Assistant
MS student 38,564 8.2% 0.25 7,877 646
Total 20,437 1,901 12,565 1,945
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
1c. Cost Share
7% of the Lead PI’s time and 25% of one graduate student assistantship with benefits valued at $14,510 is
used as cost share for year 2.
2. Services and Supplies
Communications (phone calls, faxes, mailings) $200
Materials and supplies $800
Printing and publication $1,000
MS student medical insurance $431
Subcontract to Virginia Polytechnic Institute & State University $106,959
Subcontract to University of Arkansas at Pine Bluff $51,838
Subcontract to University of Hawaii at Hilo $99,776
Subcontract to Kwame Nkrumah University, Ghana $30,349
Subcontract to Sokoine University of Agriculture, Tanzania $22,451
Total $313,804
3. Travel
International Travel
Date July 2014 Name Kwamena Quagrainie Reason for travel: Research
Departure: Indianapolis, IN USA Arrival: Accra, Ghana & Dar es Salaam, Tanzania
Ghana - Meals and Lodging @ $250/day for 10 days $2,500
Tanzania - Meals and Lodging @ $273 /day for 10 days $2,730
Airfare $3,000
Ground transport, parking, etc. $800
Miscellaneous (visa, departure tax, etc.) $200
Sub Total $9,230
48

Date June 2014 Name Graduate Student Reason for travel: Data
Collection
Departure: Indianapolis, IN USA Arrival: Dar es Salaam, Tanzania
Tanzania - Meals and Lodging @ $ 200/day for 60 days $12,000
Airfare $4,000
Ground transport, etc. $1,500
Miscellaneous (visa, departure tax, etc.) $500
Sub Total $18,000
TOTAL TRAVEL $27,230
4. Tuition
Tuition Remission: $10,893
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
36% On-campus $18,720
Rate of Indirect on Amount per
Number of Subcontracts Total Amount
Subcontracts
Subcontract
36% $25,000 2 $10,645
6. HC/Regional Cost: $217,878, which is 58% of Total Direct Cost and 54% of Total Project Cost.
49

Year 3 (10/1/14 - 9/30/15)
BUDGET JUSTIFICATION FORM
FOR US LEAD PARTNER INSTITUTIONS - PURDUE UNIVERSITY
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Benefits Rate Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Kwamena
Quagrainie
Lead PI 98,692 27.7% 0.02 1,663 461 0.07 4,829. 1,338
Graduate
Assistant
MS student 39,335 8.2% 0.5 19,668 1,613
Graduate
Assistant
MS student 39,335 8.2% 0.25 3,331 273
Total 21,331 2,073 8,260 1,611
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
1c. Cost Share
7% of the Lead PI’s time and 25% of one graduate student assistantship with benefits valued at $9,871 is
used as cost share for year 3.
2. Services and Supplies
Communications (phone calls, faxes, mailings) $100
Materials and supplies $100
Printing and publication $2,500
MS student medical insurance $432
Subcontract to Virginia Polytechnic Institute & State University $93,261
Subcontract to University of Arkansas at Pine Bluff $28,170
Subcontract to University of Hawaii at Hilo $0
Subcontract to Kwame Nkrumah University, Ghana $14,499
Subcontract to Sokoine University of Agriculture, Tanzania $22,451
Total $161,513
3. Travel
International Travel
Date May 2015
Travelers: Kwamena Quagrainie & Graduate Assistant Reason for travel: International Annual
Conference of World Aquaculture Society
Departure: Indianapolis, IN Arrival: Jeju Island, South Korea
Meals and Lodging @ $ 250/day for 8 days $2,000
Airfare $3,500
Registration $500
Miscellaneous (Ground transport, parking, visa, etc.) $1,000
Sub Total $7,000
50

Date June 2015 Name Kwamena Quagrainie Reason for travel: Research
Departure: Indianapolis, IN USA Arrival: Accra, Ghana & Dar es Salaam, Tanzania
Ghana - Meals and Lodging @ $250/day for 10 days $2,500
Tanzania - Meals and Lodging @ $273 /day for 5 days $1,365
Airfare $3,000
Ground transport, parking, etc. $800
Miscellaneous (visa, departure tax, etc.) $200
Sub Total $7,865
TOTAL TRAVEL $14,865
4. Tuition
Tuition Remission: $11,111
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
36% On-campus
Rate of Indirect on
Subcontracts
Amount per
Subcontract
51
Number of Subcontracts Total Amount
$36% $0 $0
6. HC/Regional Cost: $111,900, which is 53% of Total Direct Cost and 50% of Total Project Cost.

Purdue Sub to UAPB
Proposal Title
Enhancing the nutritional value of tilapia
Institution
for human health University of Arkansas at Pine Bluff
Year 1, Year 2,
Subcontracting Principal Investigator Budget Period: or Year 3 Year 1
From (mm/dd/yy): 7/1/2013
Rebecca Lochmann
To (mm/dd/yy): 9/30/2013
Budget Categories US Cost US Cost-share HC/Regional HC Matching
Personnel Costs
Contr ibution Cost Contribution
Salary $ 4,500 $ 1,640
Benefits/Fringe $ 675 $ 443
Subtotal $ 5,175 $ 2,083
Supplies and Services
S& S
Subcontract to
Kwame Nkrumah
$ 1,825
University of Science
and Technology,
Ghana
$ 9,993 $ 9,993 $ 8,250
Subtotal $ 11,818
Travel
Domestic $ 1,000
International $ 2,000
Subtotal $ 3,000
Equipment
Tuition
Total Direct Cost $ 19,993
Indirect Costs
Standard Indirect $ - $ 6,000
Indirect on
$ 6,000
Subcontracts
Subtotal $ 12,000
Total $ 19,993 $ 14,083 $ 9,993 $ 8,250
Additional US Non-
Federal Cost Share from
US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Single-Year Subcontracting US I nstitution Budget
GRAND TOTAL $ 19,993 $ 14,083 $ 9,993 $ 8,250
52

Proposal Title
Enhancing the nutritional value of tilapia
Institution
for human health University of Arkansas at Pine Bluff
Year 1, Year 2,
Subcontracting Principal Investigator Budget Period: or Year 3 Year 2
From (mm/dd/yy): 10/1/2013
Rebecca Lochmann
To (mm/dd/yy): 9/30/2014
Budget Categories US Cost US Cost-share HC/Regional HC Matching
Personnel Costs
Contr ibution Cost Contr ibution
Salary $ 9,000 $ 1,640
Benefits/Fringe $ 1,350 $ 443
Subtotal $ 10,350 $ 2,083
Supplies and Services
S& S
Subcontract to
Kwame Nkrumah
$ 8,650
University of Science
and Technology,
Ghana
$ 31,838 $ 31,838 $ 11,550
Subtotal $ 40,488
Travel
Domestic $ 1,000
International
Subtotal $ 1,000
Equipment
Tuition
Total Direct Cost $ 51,838
Indirect Costs
Standard Indirect $ - $ 12,000
Indirect on
$ 9,000
Subcontracts
Subtotal $ 51,838 $ 21,000
Total $ 51,838 $ 23,083 $ 31,838 $ 11,550
Additional US Non-
Federal Cost Share from
US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Single-Year Subcontracting US I nstitution Budget
GRAND TOTAL $ 51,838 $ 23,083 $ 31,838 $ 11,550
53

Proposal Title
Enhancing the nutritional value of tilapia
Institution
for human health University of Arkansas at Pine Bluff
Year 1, Year 2,
Subcontracting Principal Investigator Budget Period: or Year 3 Year 3
From (mm/dd/yy): 10/1/2014
Rebecca Lochmann
To (mm/dd/yy): 9/30/2015
Budget Categories US Cost US Cost-share HC/Regional HC Matching
Personnel Costs
Contr ibution Cost Contr ibution
Salary $ 4,500 $ 1,640
Benefits/Fringe $ 675 $ 443
Subtotal $ 5,175 $ 2,083
Supplies and Services
S& S
Subcontract to
Kwame Nkrumah
$ 3,825
University of Science
and Technology,
Ghana
$ 18,170 $ 18,170 $ 11,550
Subtotal $ 21,995
Travel
Domestic $ 1,000
International
Subtotal $ 1,000
Equipment
Tuition
Total Direct Cost $ 28,170
Indirect Costs
Standard Indirect $ - $ 6,000
Indirect on
Subcontracts
Subtotal $ 28,170 $ 6,000
Total $ 28,170 $ 8,083 $ 18,170 $ 11,550
Additional US Non-
Federal Cost Share from
US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Single-Year Subcontracting US I nstitution Budget
GRAND TOTAL $ 28,170 $ 8,083 $ 18,170 $ 11,550
54

Proposal Title
Enhancing the nutritional value of
Institution
tilapia for human health University of Arkansas at Pine Bluff
Subcontracting Principal Investigator
Budget Period: Combined Years
From (mm/dd/yy): 7/1/2013
Rebecca Lochmann
To (mm/dd/yy): 9/30/2015
Budget Categories US Cost US Cost-share HC/Regional
Personnel Costs
Contribution Cost
Salary $ 18,000 $ 4,920
Benefits/Fringe $ 2,700 $ 1,329
Subtotal $ 20,700 $ 6,249
55
HC Matching
Contr ibution
Supplies and Services
S& S
$ 14,300
Subcontract to HC
or other US Inst #1
$ 60,001 $ 60,001 $ 31,350
Subtotal $ 95,000
Travel
Domestic $ 3,000
International $ 2,000
Subtotal $ 5,000
Equipment
Tuition
Total Direct Cost $ 100,001
Indirect Costs
Standard Indirect $ 24,000
Indirect on
$ 15,000
Subcontracts
Subtotal $ 100,001 $ 45,249
Total $ 100,001 $ 45,249 $ 60,001 $ 31,350
Additional US Non-
Federal Cost Share from
US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Combined-Year Subcontracting US I nstitution Budget
GRAND TOTAL $ 100,001 $ 45,249 $ 60,001 $ 31,350

AQUAFISH INNOVATION LAB
BUDGET JUSTIFICATION INSTRUCTIONS & FORM
FOR SUBCONTRACTING US INSTITUTIONS – UNIVERSITY OF ARKANSAS AT PINE BLUFF
Specify Year 1 (3 months)
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Tom
Kasiga
Lab
technicia
n
$12.76 /
hour
Benefits
Rate
Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefit
s
15% N/
A
4500 675
Rebecca
Lochmann
Professor $82,000 27% 0.02 1640 443
Total 4500 675 0.02 1640 443
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
1c. Cost Share
Describe non-federal cost-share not outlined above under Salary Information.
US non-federal cost share is $6000, which (when combined with salary cost-share) is at least 20% of the total,
unless otherwise specified by the Lead. HC match is $8250, unless otherwise specified by the Lead. Overall
proposal costs show the US and HC match totals at least 40% of the requested direct costs.
2. Services and Supplies
Diet ingredients and chemicals for nutrition analysis $1200
Shipping charges US to Ghana $625
Subcontract to KNUST $9993
Total $11,818
3. Travel
Domestic Travel
Date July Name Rebecca Lochmann Reason for travel: Pre-synthesis meeting
Departure Location: Little Rock Arrival Location: Washington DC
Meals and Lodging @ $50 /day for 4 days $ 200
Airfare and ground transportation $ 800
Registration $
TOTAL $ 1000
International Travel
Date TBA Name Rebecca Lochmann Reason for travel: Ghana PI meeting
Departure Location: Little Rock Arrival Location: Ghana
Meals and Lodging @ $50 /day for 6 days $ 300
Airfare $1700
Airport transport, parking, etc. $
Miscellaneous (departure tax, etc.) $
TOTAL $ 2000
56

4. Tuition
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
Unrecovered indirect cost on UAPB On-campus- rate is 60% of $6000
part of funds is used as matching direct costs
Rate of Indirect on Amount per
Number of Subcontracts Total Amount
Subcontracts
Subcontract
60% $6,000 1 $6,000
6. HC/Regional Cost $9,993
57

AQUAFISH INNOVATION LAB
BUDGET JUSTIFICATION INSTRUCTIONS & FORM
FOR SUBCONTRACTING US INSTITUTIONS – UNIVERSITY OF ARKANSAS AT PINE BLUFF
Specify Year 2
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual Benefits Project Funds Cost Share Funds
Salary Rate
FTE Salary Benefits FTE Salary Benefit
s
Tom Lab $12.76 / 15% N/ 9000 1350
Kasiga technician hour
A
Rebecca
Lochmann
Professor $82,000 27% 0.02 1640 443
Total 9000 1350 0.02 1640 443
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
1c. Cost Share
Describe non-federal cost-share not outlined above under Salary Information.
US non-federal cost share is $12000, which (when combined with salary cost-share) is at least 20% of the total,
unless otherwise specified by the Lead. HC match is $11,550, unless otherwise specified by the Lead. Overall
proposal costs show the US and HC match totals at least 40% of the requested direct costs.
2. Services and Supplies
Diet ingredients and chemicals for nutrition analysis $2200
Glassware and aquaria replacement $3000
Shipping charges US to Ghana, & within the US $1625
Fish and water quality reagents $1825
Subcontract to KNUST $31,838
Total $40,488
3. Travel
Domestic Travel
Date February Name Rebecca Lochmann Reason for travel: Aquaculture America
Departure Location: Little Rock Arrival Location: Seattle
Meals and Lodging @ $50 /day for 5 days $ 250
Airfare and ground transportation $ 500
Registration $ 250
TOTAL $ 1000
International Travel
Date Name Reason for travel:
Departure Location: Arrival Location:
Meals and Lodging @ $ /day for days $
Airfare $
Airport transport, parking, etc. $
58

Miscellaneous (departure tax, etc.) $
TOTAL $
4. Tuition
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
Unrecovered indirect cost on UAPB On-campus- rate is 60% of $12,000
part of funds is used as matching direct costs
Rate of Indirect on Amount per
Number of Subcontracts Total Amount
Subcontracts
Subcontract
60% $9000 1 $9000
6. HC/Regional Cost $31,838
59

AQUAFISH INNOVATION LAB
BUDGET JUSTIFICATION INSTRUCTIONS & FORM
FOR SUBCONTRACTING US INSTITUTIONS – UNIVERSITY OF ARKANSAS AT PINE BLUFF
Specify Year 3
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual Benefits Project Funds Cost Share Funds
Salary Rate
FTE Salary Benefits FTE Salary Benefit
s
Tom Lab $12.76 15% N/A 4500 675
Kasiga technician /hour
Rebecca
Lochmann
Professor $82,000 27% 0.02 1640 443
Total 4500 675 0.02 1640 443
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
1c. Cost Share
Describe non-federal cost-share not outlined above under Salary Information.
US non-federal cost share is $6000, which (when combined with salary cost-share) is at least 20% of the total,
unless otherwise specified by the Lead. HC match is $11,550, unless otherwise specified by the Lead. Overall
proposal costs show the US and HC match totals at least 40% of the requested direct costs.
2. Services and Supplies
Diet ingredients and chemicals for nutrition analysis $1200
Shipping charges US to Ghana & within US $625
Fish and water quality reagents $2000
Subcontract to KNUST $18,170
Total $ 21,995
3. Travel
Domestic Travel
Date July Name Rebecca Lochmann Reason for travel: Aquaculture America
Departure Location: Little Rock Arrival Location: New Orleans
Meals and Lodging @ $ 50 /day for 6 days $ 300
Airfare and ground transportation $ 300
Registration $ 600
TOTAL $ 1000
International Travel
Date Name Reason for travel:
Departure Location: Arrival Location:
Meals and Lodging @ $ /day for days $
Airfare $
Airport transport, parking, etc. $
Miscellaneous (departure tax, etc.) $
TOTAL $
60

4. Tuition
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
Unrecovered indirect cost on UAPB On-campus- rate is 60% of $6000
part of funds is used as matching direct costs
Rate of Indirect on
Subcontracts
Not requested
Amount per
Subcontract
6. HC/Regional Cost $18,170
61
Number of Subcontracts Total Amount

Purdue Sub to UHH
Proposal Title
Aquaculture Production and Health-
Institution
Ghana and Tanzania University of Hawaii Hilo
Year 1, Year 2,
Subcontracting Principal Investigator Budget Period: or Year 3
From (7/1/13):
(select one)
Maria Haws
To (9/30/13): Year 1
Budget Categories US Cost US Cost-share HC/Regional HC Matching
Personnel Costs
Contr ibution Cost Contribution
Salary $ 3,500 $ 6,800
Benefits/Fringe $ 1,547 $ 2,788
Subtotal $ 5,047 $ 9,588
Supplies and Services
S& S $ -
Subcontract to HC or
other US Inst #1
$ 4,416 $ 3,874 $ -
Subtotal $ 4,416 $ 3,874 $ -
Travel
Domestic
International $ 3,250
Subtotal $ 3,250
Equipment
Tuition
Total Direct Cost $ 12,713 $ 9,588 $ 3,874 $ -
Indirect Costs
Standard Indirect $ 1,709 $ 1,975
Indirect on
Subcontracts
$ 798 $ 542
Subtotal $ 2,507 $ 1,975 $ 542
Total $ 15,221 $ 11,563 $ 4,416 $ -
Additional US Non-
Federal Cost Share from
US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Single-Year Subcontracting US I nstitution Budget
GRAND TOTAL $ 15,221 $ 11,563 $ 4,416 $ -
62

Proposal Title
Aquaculture Production and Health-
Institution
Ghana and Tanzania University of Hawaii Hilo
Year 1, Year 2,
Subcontracting Principal Investigator Budget Period: or Year 3
From (10/1/13):
(select one)
Maria Haws
To (9/30/14): Year 2
Budget Categories US Cost US Cost-share HC/Regional HC Matching
Personnel Costs
Contr ibution Cost Contribution
Salary $ 15,500 $ 6,800
Benefits/Fringe $ 2,687 $ 2,788
Subtotal $ 18,187 $ 9,588
Supplies and Services
S& S $ 5,000
Subcontract to HC or
other US Inst #1
$ 60,637 $ 53,190 $ 24,723
Subtotal $ 65,637 $ 53,190 $ 24,723
Travel
Domestic
International $ 5,750
Subtotal $ 5,750
Equipment
Tuition
Total Direct Cost $ 89,574 $ 9,588 $ 53,190 $ 24,723
Indirect Costs
Standard Indirect $ 5,961 $ 1,975
Indirect on
Subcontracts
$ 4,240 $ 7,447
Subtotal $ 10,201 $ 1,975 $ 7,447
Total $ 99,776 $ 11,563 $ 60,637 $ 24,723
Additional US Non-
Federal Cost Share from
US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Single-Year Subcontracting US I nstitution Budget
GRAND TOTAL $ 99,776 $ 11,563 $ 60,637 $ 24,723
64

Proposal Title
Aquaculture Production and Health-
Institution
Ghana and Tanzania University of Hawaii Hilo
Year 1, Year 2, or
Subcontracting Principal Investigator Budget Period: Year 3
From (10/1/12):
(select one)
Maria Haws
To (9/30/14): Cumulative
Budget Categories US Cost US Cost-share HC/Regional HC Matching
Personnel Costs
Contr ibution Cost Contr ibution
Salary $ 19,000 $ 13,600
Benefits/Fringe $ 4,234 $ 5,576
Subtotal $ 23,234 $ 19,176
Supplies and Services
S& S $ 5,000
Subcontract to HC -
WIOMSA
$ 65,053
$ 57,064 $ 24,723
Subtotal $ 70,053 $ 57,064 $ 24,723
Travel
Domestic
International $ 9,000
Subtotal $ 9,000
Equipment
Tuition
Total Direct Cost $ 102,287 $ 19,176 $ 57,064 $ 24,723
Indirect Costs
Standard Indirect $ 7,671 $ 3,950
Indirect on
Subcontracts
$ 5,150
$ -
$ 7,989
Subtotal $ 12,821 $ 3,950 $ 7,989
Total $ 115,108 $ 23,126 $ 65,053 $ 24,723
Additional US Non-
Federal Cost Share from
US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Cumulative Subcontracting US I nstitution Budget
GRAND TOTAL $ 115,108 $ 23,126 $ 65,053 $ 24,723
66

AQUAFISH INNOVATION LAB
BUDGET JUSTIFICATION INSTRUCTIONS & FORM
FOR SUBCONTRACTING US INSTITUTIONS – UNIVERSITY OF HAWAII AT HILO
Year 1
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Maria
Haws
Assoc.
Professor
84,000 44.21%
regular
41% for
match
Benefits Rate Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
0.04
16
3500 1547 0.08 6800 2788
Total 3500 1547 6800 2788
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
TBA Female TBA M.S.
1c. Cost Share
Cost share from salary and fringe benefits is $9588. Indirect charges on this at a rate of 20.6% are: $1975, for a
total cost share of $11,563 for Year 1.
US non-federal cost share is $11,563, which is at least 20% of the total, unless otherwise specified by the Lead. HC
match is $0 in year 1, unless otherwise specified by the Lead. Project averages are 20% UHH and 20% WIOMSA.
Overall proposal costs show the US and HC match totals at least 40% of the requested direct costs.
2. Services and Supplies ($0)
3. Travel ($3250)
Domestic Travel-none
Date Name Reason for travel:
Departure Location: Arrival Location:
Meals and Lodging @ $ /day for days $
Airfare and ground transportation $
Registration $
TOTAL $
International Travel
Date TBA
Name Maria Haws
Reason for travel: provide technical assistance and/or report on project finding
Departure Location: Hilo, HI
Arrival Location: Ghana, Washington DC or otherwise specified by CRSP ME
Meals and Lodging @ $300 /day for 6.6 days $2000
Airfare $1250
Airport transport, parking, etc. $
Miscellaneous (departure tax, etc.) $
TOTAL $3250
67

4. Tuition
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
20.6% on $8,297 of direct costs Off campus $11,563
Rate of Indirect on
Subcontracts
Amount per
Subcontract
68
Number of Subcontracts Total Amount
6. HC/Regional Cost
HC cost is for subcontract to WIOMSA for $4416 and for indirect on this for $910 for a total of $5,326.

AQUAFISH INNOVATION LAB
BUDGET JUSTIFICATION INSTRUCTIONS & FORM
FOR SUBCONTRACTING US INSTITUTIONS – UNIVERSITY OF HAWAII AT HILO
Year 2
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Maria
Haws
Assoc.
Professor
Benefits Rate Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
84,000 44.21% 0.04 3500 1547 0.08 6800 2788
regular
41% for
match
16
12,000 9.5% 1.0 12000 1140
TBA Graduate
student
Total 15500 2687 6800 2788
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
TBA Female TBA M.S.
1c. Cost Share
Cost share from salary and fringe benefits is $9588. Indirect charges on this at a rate of 20.6% are: $1975, for a total
cost share of $11,563 for Year 2 from UHH.
US non-federal cost share is $11,563, which is at least 20% of the total, unless otherwise specified by the Lead. HC
match is $24,724 in year 1, unless otherwise specified by the Lead. Project averages are 20% UHH and 20%
WIOMSA. Overall proposal costs show the US and HC match totals at least 40% of the requested direct costs.
2. Services and Supplies ($5,000)
3. Travel ($5,750)
Domestic Travel-none
Date Name Reason for travel:
Departure Location: Arrival Location:
Meals and Lodging @ $ /day for days $
Airfare and ground transportation $
Registration $
TOTAL $
International Travel (5,750)
Date TBA
Name Maria Haws
Reason for travel: provide technical assistance and/or report on project finding
Departure Location: Hilo, HI
Arrival Location: Zanzibar, Tanzania
Meals and Lodging @ $250 /day for 7 days $1750
Airfare $3500
Airport transport, parking, etc. $250
Miscellaneous (departure tax, etc.) $250
TOTAL $5,750
69

4. Tuition
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
20.6% on $28,937 of direct UHH costs Off campus $10,201
Rate of Indirect on
Subcontracts
20.5%
Note: indirect is charged
only on first $25,000 of
subcontracts
6. HC/Regional Cost
HC cost is for subcontract to WIOMSA for $60,637.
Amount per
Subcontract
Number of Subcontracts Total Amount
$4,240 1 4,240
70

Purdue Sub to VT
Seect Proposal gTitle bette adapted st a s o
species and evaluating Ghana's
at e Institution
aquaculture development plan for the
changing climate Virginia Polytechnic Institute & State University
Subcontracting Principal Investigator Budget Period: Year 1
From (mm/dd/yy): 7/1/2013
Emmanuel Frimpong
To (mm/dd/yy): 9/30/2013
Budget Categories US Cost US Cost-share HC/Regional
Contr ibution Cost
71
HC Matching
Contr ibution
Personnel Costs
Salary $ 11,240 $ 3,515 $ 5,830
Benefits/Fringe $ 893 $ 1,028
Subtotal $ 12,133 $ 4,543 $ 5,830
Supplies and Services $ -
S& S $ -
Subcontract to
KNUST
$ 15,000 $ 15,000
Subtotal $ 15,000 $ 15,000
Travel
Domestic $ -
International $ 2,500
Subtotal $ 2,500
Equipment $ -
Tuition $ -
Total Direct Cost $ 29,633 $ 4,543 $ 15,000 $ 5,830
Indirect Costs
Standard Indirect $ 3,805 $ 1,181
Indirect on
Subcontracts
$ 3,900
Subtotal $ 7,705 $ 1,181 $ -
Total $ 37,338 $ 5,724 $ 15,000 $ 5,830
Additional US Non-
Federal Cost Share from
US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Single-Year Subcontracting US I nstitution Budget
GRAND TOTAL $ 37,338 $ 5,724 $ 15,000 $ 5,830

Selecting Proposal Title better adapted strains of native Institution
species and evaluating Ghana's
aquaculture development plan for the
changing climate Virginia Polytechnic Institute & State University
Subcontracting Principal Investigator Budget Period: Year 2
From (mm/dd/yy): 10/1/2013
Emmanuel Frimpong
To (mm/dd/yy): 9/30/2014
Budget Categories US Cost US Cost-share HC/Regional
Contr ibution Cost
72
HC Matching
Contr ibution
Personnel Costs
Salary $ 32,710 $ 5,436 $ 12,265
Benefits/Fringe $ 2,716 $ 1,590
Subtotal $ 35,426 $ 7,026 $ 12,265
Supplies and Services $ 1,000
S& S $ 1,000
Subcontract to
KNUST
$ 47,500 $ 47,500
Subtotal $ 48,500 $ 47,500
Travel
Domestic $ 3,200
International $ 5,500
Subtotal $ 8,700
Equipment $ -
Tuition $ - $ 11,498
Total Direct Cost $ 92,626 $ 18,524 $ 47,500 $ 12,265
Indirect Costs
Standard Indirect $ 11,733 $ 1,827
Indirect on
Subcontracts
$ 2,600
Subtotal $ 14,333 $ 1,827 $ -
Total $ 106,959 $ 20,351 $ 47,500 $ 12,265
Additional US Non-
Federal Cost Share from
US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Single-Year Subcontracting US I nstitution Budget
GRAND TOTAL $ 106,959 $ 20,351 $ 47,500 $ 12,265

Selecting Proposal Title better adapted strains of native Institution
species and evaluating Ghana's
aquaculture development plan for the
changing climate Virginia Polytechnic Institute & State University
Subcontracting Principal Investigator Budget Period: Year 3
From (mm/dd/yy): 10/1/2014
Emmanuel Frimpong
To (mm/dd/yy): 9/30/2015
Budget Categories US Cost US Cost-share HC/Regional
Contr ibution Cost
73
HC Matching
Contr ibution
Personnel Costs
Salary $ 34,018 $ 5,680 $ 2,620
Benefits/Fringe $ 2,824 $ 1,661
Subtotal $ 36,842 $ 7,341 $ 2,620
Supplies and Services $ 1,000
S& S $ 1,000
Subcontract to
KNUST
$ 35,500 $ 35,500
Subtotal $ 36,500 $ 35,500
Travel
Domestic $ 3,500
International $ 4,500
Subtotal $ 8,000
Equipment $ -
Tuition $ - $ 12,188
Total Direct Cost $ 81,342 $ 19,529 $ 35,500 $ 2,620
Indirect Costs
Standard Indirect $ 11,919 $ 1,909
Indirect on
Subcontracts
$ -
Subtotal $ 11,919 $ 1,909 $ -
Total $ 93,261 $ 21,438 $ 35,500 $ 2,620
Additional US Non-
Federal Cost Share from
US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Single-Year Subcontracting US I nstitution Budget
GRAND TOTAL $ 93,261 $ 21,438 $ 35,500 $ 2,620

Subcontracting Principal Investigator Budget Period: Combined Years
From (mm/dd/yy): 7/1/2013
Emmanuel Frimpong
To (mm/dd/yy): 9/30/2015
Budget Categories US Cost US Cost-share HC/Regional
Contr ibution Cost
74
HC Matching
Contr ibution
Personnel Costs
Salary $ 77,968 $ 14,631 $ 20,715
Benefits/Fringe $ 6,433 $ 4,279 $ -
Subtotal $ 84,401 $ 18,910 $ 20,715
Supplies and Services $ 2,000
S& S $ 2,000
Subcontract to KNUST $ 98,000 $ 98,000
Subtotal $ 100,000 $ 98,000
Travel
Domestic $ 6,700
International $ 12,500
Subtotal $ 19,200
Equipment $ -
Tuition $ - $ 23,686
Total Direct Cost $ 203,601 $ 42,596
Indirect Costs
Standard Indirect $ 27,456 $ 4,917
Indirect on
$ 6,500
Subcontracts
Subtotal $ 33,956 $ 4,917
Total $ 237,557 $ 47,513 $ 98,000 $ 20,715
Additional US Non-Federal
Cost Share from US Inst #1
BUDGET FORM
AquaFish Innovation Lab
Combined-Year Subcontracting US I nstitution Budget
Proposal Title Institution
Selecting better-adapted strains of native
species and evaluating Ghana's aquaculture
development plan for the changing climate Virginia Polytechnic Institute & State University
GRAND TOTAL $ 237,557 $ 47,513 $ 98,000 $ 20,715

Specify Year 1 (3 months)
AQUAFISH INNOVATION LAB
BUDGET JUSTIFICATION FORM FOR SUBCONTRACTING US INSTITUTIONS
VIRGINIA TECH
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual Salary Benefits
Rate
Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Emmanuel Associate $75,320 (AY) 29.25% 0.11 8,369 649 0.05 3,515 1,028
Frimpong
Professor
Iris Fynn GRA $24,006 (CY) 8.50% 0.125 2,871 244 0 0 0
Total $11,240 $893 $3,515 $1,028
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
Iris Fynn Female Ghana MS
1c. Cost Share
5% of US PI salary and fringe valued at $4,543 is used as a match.
2. Services and Supplies
3. Travel
Domestic Travel
International Travel
Date: July Name: Emmanuel Frimpong
Reason for travel: Africa Regional meeting/CRSP council Meeting/First Evaluation Workshop/
& reconnaissance field surveys
Departure Location: Roanoke, VA Arrival Location: Accra, Ghana
Meals and Lodging @ $ 167 /day for 15 days $ 2,500
Airfare $ (covered under a separate budget)
Airport transport, parking, etc. $ (covered under a separate budget)
Miscellaneous (departure tax, etc.) $ (covered under a separate budget)
TOTAL $ 2,500
4. Tuition
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
26%
Rate of Indirect on
Subcontracts
26% on first 25% of $98,000
subcontract
Amount per
Subcontract
$3,900
Off-campus
6. HC/Regional Cost $15,000
75
$7,705
Number of Subcontracts Total Amount
1
$3,900

Specify Year 2
BUDGET JUSTIFICATION FORM FOR SUBCONTRACTING US INSTITUTIONS
VIRGINIA TECH
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual Salary Benefits
Rate
Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Emmanuel Associate $75,320 (AY) 29.25% 0.11 8,704 675 0.07 5,436 1,590
Frimpong
Professor
Iris Fynn GRA $24,006 (CY) 8.50% 1 24,006 2,041 0 0 0
Total $32,710 $2,716 $5,436 $1,590
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
Iris Fynn Female Ghana MS
1c. Cost Share
7% of US PI salary and fringe valued at $7,026 is used as a match. In addition, one academic year tuition of
$11,498 is waived for graduate student and unrecovered indirect on cost-shared funds ($1,827) for a total of
$20,351 is used as a match in year 2.
2. Services and Supplies
$1,000 is requested for miscellaneous supplies such as waders that the Virginia Tech crew uses in the field
in Ghana.
3. Travel
Domestic Travel #1
Date: February 2014 Name: Emmanuel Frimpong
Reason for travel: Presentation of research and participation in Aquafish Program Meeting at
WAS, Seattle
Departure Location: Roanoke, VA Arrival Location: Seattle, Washington
Registration $ 400
Meals and Lodging @ $200/day for 4 days $ 800
Airfare $ 500
Airport transport, parking, etc. $ 0
Miscellaneous (departure tax, etc.) $ 0
Sub-total $1,700
Domestic Travel #2
Date: February 2014 Name: Iris Fynn
Reason for travel: Presentation of research
Departure Location: Roanoke, VA Arrival Location: Seattle, Washington
Registration $ 200
Meals and Lodging @ $200/day for 4 days $ 800
Airfare $ 500
Airport transport, parking, etc. $ 0
Miscellaneous (departure tax, etc.) $ 0
Sub-total $1,500
International Travel
76

Date: July 2014 Name: Emmanuel Frimpong
Reason for travel: Lead Second Evaluation Workshop and Training, visit field sites and
participate in aspects of field work
Departure Location: Roanoke, VA Arrival Location: Accra, Ghana
Meals and Lodging @ $ 167 /day for 16 days $ 2,667
Airfare $ 2,500
Airport transport, parking, etc. $ 200
Miscellaneous (departure tax, etc.) $ 133
Sub-total $5,500
TOTAL $8,700
4. Tuition
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
26%
Rate of Indirect on
Subcontracts
26% on first 25% of $98,000
subcontract
Amount per
Subcontract
$2,600
Off-campus
6. HC/Regional Cost $47,500
77
$14,333
Number of Subcontracts Total Amount
1
$2,600

Specify Year 3
BUDGET JUSTIFICATION FORM FOR SUBCONTRACTING US INSTITUTIONS
VIRGINIA TECH
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual Salary Benefits
Rate
Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Emmanuel
Frimpong
Associate Professor $75,320 (AY) 29.25% 0.11 9,052 702 0.07 5,680 1,661
Iris Fynn GRA $24,006 (CY) 8.50% 1 24,996 2,122 0 0 0
Total $34,018 $2,824 $5,680 $1,661
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
Iris Fynn Female Ghana MS
1c. Cost Share
7% of US PI salary and fringe valued at $7,341 is used as a match. In addition, one academic year tuition of
$12,188 is waived for graduate student and unrecovered indirect on cost-shared funds ($1,909) for a total of
$21,438 is used as a match in year 3.
2. Services and Supplies
$1,000 is requested for miscellaneous supplies such as waders that the Virginia Tech crew uses in the field
in Ghana.
3. Travel
Domestic Travel #1
Date: February 2015 Name: Emmanuel Frimpong
Reason for travel: Presentation of research at WAS, New Orleans
Departure Location: Roanoke, VA Arrival Location: New Orleans, Louisiana
Registration $ 450
Meals and Lodging @ $200/day for 4 days $ 800
Airfare $ 400
Airport transport, parking, etc. $ 100
Miscellaneous (departure tax, etc.) $ 100
Sub-total $1,850
Domestic Travel #2
Date: February 2015 Name: Iris Fynn
Reason for travel: Presentation of research at WAS, New Orleans
Departure Location: Roanoke, VA Arrival Location: Seattle, Washington
Registration $ 250
Meals and Lodging @ $200/day for 4 days $ 800
Airfare $ 400
Airport transport, parking, etc. $ 100
Miscellaneous (departure tax, etc.) $ 100
Sub-total $1,650
78

International Travel
Date: July 2015 Name: Emmanuel Frimpong
Reason for travel: Lead Third Evaluation Workshop and Training, Conduct project wrap-up
activities
Departure Location: Roanoke, VA Arrival Location: Accra, Ghana
Meals and Lodging @ $ 167 /day for 16 days $ 2,171
Airfare $ 2,200
Airport transport, parking, etc. $ 100
Miscellaneous (departure tax, etc.) $ 33
Sub-total $4,500
TOTAL $8,000
4. Tuition
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
26%
Rate of Indirect on
Subcontracts
26% on first 25% of $98,000
subcontract
Amount per
Subcontract
Off-campus
6. HC/Regional Cost $35,500
$0
79
$11,919
Number of Subcontracts Total Amount
1
$0

Purdue Sub to KNUST
Proposal Title HC Institution
Development of a Cell-Phone Based Seafood Market Kwame nkrumah University of Science and
Information System (SM IS) in Ghana: A pplication to Tilapia Technology, Kumasi
HC Principal Investigator
Budget Period: Year 1
From (mm/dd/yy): 7/1/2013
Steve A misah
To (mm/dd/yy): 9/30/2013
Budget Categories Host Country Cost
HC Contribution
Personnel Costs
Salary $ 48
Benefits/Fringe $ -
Subtotal $ 48
Supplies and Services
S& S $ 10,500
Subtotal $ 10,500
Travel
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Domestic $ 2,000
International $ 3,100
Subtotal $ 5,100
Equipment $ -
Tuition $ -
Total Direct Cost $ 15,648
Indirect Costs $ -
Standard Indirect $ -
Subtotal $ -
Total $ 15,648
80
$ 4,158
$ 1,039
$ 5,197
$ 500
$ 5,697
$ -
$ -
$ -
$ 5,697

Proposal Title HC Institution
Development of a Cell-Phone Based Seafood Market Kwame nkrumah University of Science and
Information System (SM IS) in Ghana: A pplication to Tilapia Technology, Kumasi
HC Principal Investigator
Budget Period: Year 2
From (mm/dd/yy): 10/1/2013
Steve A misah
To (mm/dd/yy): 9/30/2014
Budget Categories Host Country Cost
HC Contribution
Personnel Costs
Salary $ 4,479
Benefits/Fringe $ 520
Subtotal $ 4,999
Supplies and Services
S& S $ 19,500
Subtotal $ 19,500
Travel
Domestic $ 600
International $ 5,250
Subtotal $ 5,850
Equipment $ -
Tuition $ -
Total Direct Cost $ 30,349
Indirect Costs $ -
Subtotal
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Standard Indirect $ -
Total $ 30,349
81
$ 7,758
$ 1,039
$ 8,797
$ 1,500
$ 10,297
$ 10,297

Proposal Title HC Institution
Development of a Cell-Phone Based Seafood Market Kwame nkrumah University of Science and
Information System (SM IS) in Ghana: A pplication to Tilapia Technology, Kumasi
HC Principal Investigator
Budget Period: Year 3
From (mm/dd/yy): 10/1/2014
Steve A misah
To (mm/dd/yy): 9/30/2015
Budget Categories Host Country Cost
HC Contribution
Personnel Costs
Salary $ 4,479
Benefits/Fringe $ 520
Subtotal $ 4,999
Supplies and Services
S& S $ 9,500
Subtotal $ 9,500
Travel
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Domestic $ -
International $ -
Subtotal $ -
Equipment $ -
Tuition $ -
Total Direct Cost $ 14,499
Indirect Costs $ -
Standard Indirect $ -
Subtotal $ -
Total $ 14,499
82
$ 7,758
$ 1,039
$ 8,797
$ 1,500
$ 10,297
$ 10,297

Proposal Title HC Institution
Development of a Cell-Phone Based Seafood Market Kwame nkrumah University of Science and
Information System (SM IS) in Ghana: A pplication to Tilapia Technology, Kumasi
HC Principal Investigator
Budget Period: Year 1 - 3
From (mm/dd/yy): 7/1/2013
Steve A misah
To (mm/dd/yy): 9/30/2015
Budget Categories Host Country Cost
HC Contribution
Personnel Costs
Salary $ 9,006
Benefits/Fringe $ 1,040
Subtotal $ 10,046
Supplies and Services
S& S $ 39,500
Subtotal $ 39,500
Travel
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Domestic $ 2,600
International $ 8,350
Subtotal $ 10,950
Equipment $ -
Tuition $ -
Total Direct Cost $ 60,496
Indirect Costs $ -
Standard Indirect $ -
Subtotal $ -
Total $ 60,496
83
$ 19,674
$ 3,117
$ 22,791
$ 3,500
$ 26,291
$ 26,291

BUDGET JUSTIFICATION FORM FOR HOST COUNTRY
INSTITUTIONS
Kwame Nkrumah University of Science and Technology (KNUST)
Year 1 (3 months)
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Annual Benefits
Name Title Salary Rate Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Stephen Amisah
(PI)
Associate
Professor
$41,579 25% 0.1 4,158 1,039
Yaa Tiwaa
Amoah (Miss)
GRA $4800
0 0.01
$48 $0
0 0
Total $48 $0 4,158 $1,039
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
Yaa Tiwaa Amoah (Miss) F Ghanaian MPhil
1c. Matching contributions
KNUST will provide supporting 10% of PI time valued at $5,197; Waiver of MPhil student tuition
valued at $500 for Year 1. For a total of $5,697
2. Services and Supplies
Stationery, printing accessories, duplication $500
Programing for Seafood Market information System - FarmerLine $10,000
Subtotal $10,500
3. Travel
Domestic Travel
Estimated domestic travel expenditure of $2,000 include travels of research staff and students
to study locations in Ghana for interviews and administration of the research; travels to
aquaculture production facilities, market centers, etc. These estimates also include meals and
lodging.
International Travel
Date: August 2013 Name: Stephen Amisah: Accra- Washington DC: Reason for travel
:Attend AquaFish HC &USPI meetings $2,000
Meals and Lodging @ $250/day for 4 days $1,000
Airport transport, parking, etc. $100
Miscellaneous (departure tax, etc.) $
Subtotal $3,100
4. Tuition
GRAND TOTAL $15,648
84

5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
10% 10% 0
6. HC/Regional Cost $15,648 and a match of $5,697 (representing 36.4% of cost)
85

BUDGET JUSTIFICATION FORM FOR HOST COUNTRY
INSTITUTIONS
Kwame Nkrumah University of Science and Technology (KNUST)
YEAR 2 01 OCT 2013-Sept 30, 2014
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title
Annual
Salary
Benefits
Rate Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Stephen Amisah
(PI)
Associate
Professor
$41,579 25% 0.05 $2,079 $520 0.1 4,158 1,039
Obirikorang Nana
Kwasi
Research
Assistant
$18,000 0 0 0 0.2 $3,600 $0
Yaa Tiwaa
Amoah (Miss)
GRA $4800 0 0.5 $2,400 $0
0 0
Total $4,479 $520 $7,758 $1,039
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
Yaa Tiwaa Amoah (Miss) F Ghanaian MPhil
1c. Matching contributions
KNUST will provide 10% of PI time valued at $5,197, 20% of Research Assistant time valued at $3,600;
Waiver of MPhil student tuition valued at $1,500 for a total match of $10,297 for Year 2.
2. Services and Supplies
Workshop and conference facilities & communication for training programs $4,500
Programing for Seafood Market information System - FarmerLine $15,000
Subtotal $19,500
3. Travel
Domestic Travel
Estimated domestic travel of $600 is related to any outstanding trips in field studies and PI
travels in country.
International Travel
Date: Feb. 27 - March 4 2014 Name: Stephen Amisah: Reason: Attend WAS/AquaFish
CRSP conferences
Accra-Seattle,-Accra Arrival: Feb 2014 $2,500
Meals and Lodging @ $250/day for 7 days $1,750
Airport transport, parking, etc. $ 250
Miscellaneous (visa, departure tax, etc.) $ 150
86

Conference registration $ 600
Subtotal $5,250
4. Tuition:
GRAND TOTAL 30,349
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
10% 10%
6. HC/Regional Cost $30,349 and a match of $10,297 (representing 33.9% of cost)
87

BUDGET JUSTIFICATION FORM FOR HOST COUNTRY
INSTITUTIONS
Kwame Nkrumah University of Science and Technology (KNUST)
YEAR 3: Oct. 2014-Sept 30, 2015
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Annual Benefits
Name Title Salary Rate Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Stephen Amisah
(PI)
Associate
Professor
$41,579 25% 0.05 $2,079 $520 0.1 4,158 1,039
Obirikorang Nana K Research
Assistant
$18,000 0 0 0 0.2 $3,600 $0
Yaa Tiwaa Amoah
(Miss)
GRA $4800 0 0.5 $2,400 $0
0 0
Total $4,479 $520 $7,758 $1,039
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
Yaa Tiwaa Amoah (Miss) F Ghanaian MPhil
1c. Matching contributions
KNUST will provide 10% of PI time valued at $5,197, 20% of Research Assistant time valued at $3,600;
Waiver of MPhil student tuition valued at $1,500 for a total match of $10,297 for Year 2.
2. Services and Supplies
Workshop and conference facilities & communication for training programs $4,500
Testing of Seafood Market information System - FarmerLine $5,000
Subtotal $9,500
3. Travel
Domestic Travel
International Travel
4. Tuition: Part tuition for 1 MPhil student $
GRAND TOTAL 14,499
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
10% 10%
6. HC/Regional Cost $14,499 and a match of $10,297 (representing 71.0% of cost).
88

Purdue Sub to SUA
Proposal Title:
Value chain analysis of farmed Nile tilapia
HC Institution:
(Oreochromis niloticus) and African catfish (Clarias Sokoine University of Agriculture
HC Principal Investigator
Budget Period: Year 1
Sebastian W. Chenyambuga From (mm/dd/yy): 1 July, 2013
To (mm/dd/yy): 30 September, 2013
Budget Categories Host Country Cost
HC Contribution
Personnel Costs
Salary $ 800
Benefits/Fringe $ -
Subtotal $ 800
Supplies and Services
S& S $ 548
Subtotal $ 548
Travel
Domestic $ 1,350
International $ 2,400
Subtotal $ 3,750
Equipment
Tuition $ -
Total Direct Cost $ 5,098
Indirect Costs
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Standard Indirect $ -
Subtotal $ -
Total $ 5,098 $ 8,160
89
$ 8,160
$ -
$ 8,160
$ -

(Oreochromis niloticus) and African catfish (Clarias Sokoine University of Agriculture
HC Principal Investigator
Budget Period: Year 2
Sebastian W. Chenyambuga From (mm/dd/yy): 1 October, 2013
To (mm/dd/yy): 30 September, 2014
Budget Categories Host Country Cost
Personnel Costs
Salary $ 4,800
Benefits/Fringe $ -
Subtotal $ 4,800
Supplies and Services
S& S $ 4,221
Subtotal $ 4,221
Travel
Domestic $ 6,750
International $ 5,180
Subtotal $ 11,930
Equipment
Tuition $ 1,500
Total Direct Cost $ 22,451
Indirect Costs
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title: HC Institution:
Value chain analysis of farmed Nile tilapia
Standard Indirect $ -
Subtotal $ -
Total $ 22,451
90
HC Contribution
$ 8,160
$ -
$ 8,160
$ 8,160

HC Principal Investigator
Budget Period: Year 3
Sebastian W. Chenyambuga From (mm/dd/yy): 1 October, 2014
To (mm/dd/yy): 30 September, 2015
Budget Categories Host Country Cost
HC Contribution
Personnel Costs
Salary $ 4,800
Benefits/Fringe $ -
Subtotal $ 4,800
Supplies and Services
S& S $ 4,221
Subtotal $ 4,221
Travel
Domestic $ 6,750
International $ 5,180
Subtotal $ 11,930
Equipment
Tuition $ 1,500
Total Direct Cost $ 22,451
Indirect Costs
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title:Value chain analysis of farmed Nile tilapHC Institution: Sokoine University of Agriculture
Standard Indirect $ -
Subtotal $ -
Total $ 22,451
91
$ 8,160
$ -
$ 8,160
$ -
$ 8,160

BUDGET FORM
AquaFish Innovation Lab
Proposal Title:
Combined-Year HOST COUNTRY Institution Budget
HC Institution:
Value chain analysis of farmed Nile tilapia (Oreochromis
niloticus) and African catfish (Clarias gariepinus) in Tanzania Sokoine University of Agriculture
HC Principal Investigator
Budget Period: Combined Years
Sebastian Chenyambuga From (mm/dd/yy): 1-Jul-13
To (mm/dd/yy): 30-Sep-15
Budget Categories Host Country Cost
HC Contribution
Personnel Costs
Salary $ 10,400
Benefits/Fringe $ -
Subtotal $ 10,400
Supplies and Services
S&S $ 8,990
Subtotal $ 8,990
Travel
Domestic $ 14,850
International $ 12,760
Subtotal $ 27,610
Equipment $ -
Tuition $ 3,000
Total Direct Cost $ 50,000
Indirect Costs
Standard Indirect $ -
Subtotal $ -
Total $ 50,000 $ 26,480
92
$ 24,480
$ -
$ 24,480
$ -
$ 2,000

BUDGET JUSTIFICATION FORM FOR HOST COUNTRY INSTITUTIONS
SOKOINE UNIVERSITY OF AGRICULTURE (SUA)
Specify Year 1 (3 months)
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Benefits
Rate
93
Project Funds Cost Share Funds
FTE Salary* Benefits FTE Salary Benefits
Sebastian
Chenyambuga
HC PI 20,400 0 0.03 600 0 0.2 4,080 0
Nazael Madalla HC co-PI 20,400 0 0.2 4,080 0
Tausi Ally Graduate
student
2,400 0 0.08 200 0 0 0 0
Total 5300 800 8,160
1b. Student Information
Name Gender Nationality Degree sought
Tausi Ally Female Tanzanian MSC
1c. Matching Contributions
22% of HC PI Salary valued at US$ 8,160 is used for match.
2. Services and supplies
Various stationery and office supplies US$ 85
Survey materials US$463
TOTAL US$548
3. TRAVEL
Domestic travel
Date: August 2013: Name: Sebastian Chenyambuga and Nazael madalla
Location: Morogoro and Coastal regions,Tanzania
Car fuel 500 litres @ US$1.5 = US$ 750
Accommodation and meals 10 days @ US$ 60 = 600
Total = US$ 1350
International travel
Date: July 2013
Reason for travel: To attend pre-synthesis workshop and inception meeting
Departure location: Dar es Salaam, Tanzania Arrival location: Accra, Ghana
Air fare: US$1150
Accommodation and meals: 5 days @ US$ 200 = US$ 1000

Local transportation in Tanzania: US$ 130
Ground transportation in Ghana: 120
Total = US$ 2400
4. Tuition
5. Indirect costs
Standard indirect cost (Institutional charge is 10% of the total cost) Waived
94

Specify Year 2
BUDGET JUSTIFICATION FORM FOR HOST COUNTRY INSTITUTIONS
SOKOINE UNIVERSITY OF AGRICULTURE (SUA)
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Sebastian
Chenyambuga
Nazael
Madalla
Benefits
Rate
95
Project Funds Cost Share Funds
FTE Salary* Benefits FTE Salary Benefits
HC PI 20,400 0 0.12 2400 0 0.2 4,080 0
HC co-PI 20,400 0.2 4,080
Tausi Ally Graduate
student
2400 0 0.5 1200 0 0 0 0
TBD Graduate
student
2400 0.5 1200
Total 22,800 4800 8,160
1b. Student Information
Name Gender Nationality Degree sought
Tausi Ally
TBD
Female Tanzanian MSC
1c. Matching Contributions
22% of HC PIs valued at US$ 8,160 will be used as match.
2. Services and supplies
Various stationery and office supplies US$ 300
Workshop Expenses US$ 1880
Survey Expenses US$2,041
TOTAL US$4,221
3. TRAVEL
Domestic travel
Date: November – December 2013: Name: Sebastian Chenyambuga and Nazael madalla
Location: Mbeya and Arusha ,Tanzania
Car fuel 900 litres @ US$1.5 = US$ 1350
Accommodation and meals 18 days @ US$ 60 = 5400
Total = US$ 6750

International travel
Date: February 2014
Reason for travel: To attend WAS conference and AquaFish Innovative Lab meeting
Departure location: Dar es Salaam, Tanzania Arrival location: Seattle, USA
Air fare: US$3500
Accommodation and meals: 5 days @ US$ 250 = US$ 1250
Local transportation in Tanzania: US$ 130
Ground transportation in US: 300
Total = US$ 5180
4. Tuition US$ 1500
5. Indirect costs
Standard indirect cost (Institutional charge is 10% of the total cost) Waived
96

Specify Year 3
BUDGET JUSTIFICATION FORM FOR HOST COUNTRY INSTITUTIONS
SOKOINE UNIVERSITY OF AGRICULTURE (SUA)
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Benefits
Rate
97
Project Funds Cost Share Funds
FTE Salary* Benefits FTE Salary Benefits
Sebastian
Chenyambuga
HC PI 20,400 0 0.12 2400 0 0.2 4,080 0
Nazael Madalla HC co-PI 20,400 0.2 4,080
Tausi Ally Graduate
student
2400 0 0.5 1200 0 0 0 0
TBD Graduate
student
2400 0.5 1200
Total 22,800 4800 8,160
1b. Student Information
Name Gender Nationality Degree sought
Tausi Ally
TBD
Female Tanzanian MSC
1c. Matching Contributions
22% of HC PIs valued at US$ 8,160 will be used as match.
2. Services and supplies
Workshop Expenses US$2,180
Survey Expenses US$2,041
TOTAL US$4,221
3. TRAVEL
Domestic travel
Date: November – December 2013: Name: Sebastian Chenyambuga and Nazael madalla
Location: Mbeya and Arusha ,Tanzania
Car fuel 900 litres @ US$1.5 = US$ 1350
Accommodation and meals 18 days @ US$ 60 = 5400
Total = US$ 6750
International travel
Date: February 2014

Reason for travel: To attend WAS conference and AquaFish Innovative Lab meeting
Departure location: Dar es Salaam, Tanzania Arrival location: Seattle, USA
Air fare: US$3500
Accommodation and meals: 5 days @ US$ 250 = US$ 1250
Local transportation in Tanzania: US$ 130
Ground transportation in US: 300
Total = US$ 5180
4. Tuition US$ 1500
5. Indirect costs
Standard indirect cost (Institutional charge is 10% of the total cost) Waived
98

UAPB Sub to KNUST
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title HC Institution
Enhancing Tilapia diets to improve source of n-3 Kwamw Nkrumah univerisyt of Science and
fatty acids to humans in Ghana
Technology, Ghana
HC Principal Investigator
Budget Period: Year 1
Regina Edziyie From (mm/dd/yy): 7/2/2013
To (mm/dd/yy): 9/30/2013
Budget Categories
Personnel Costs
Host Country Cost
HC Contribution
Salary $ 4,800 $ 7,200
Benefits/Fringe $ 600 $ 300
Subtotal $ 5,400 $ 7,500
Supplies and Services
S& S $ 2,380
Subtotal $ 2,380
Travel
Domestic $ 850
International $ -
Subtotal $ 850
Equipment $ -
Tuition $ 500
Total Direct Cost $ 9,130
Indirect Costs $ 0
Standard Indirect $ 863
Subtotal $ 863
Total $ 9,993
99
$ 7,500
$ -
$ 750
$ 750
$ 8,250

BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title HC Institution
Enhancing Tilapia diets to improve source of n-3 Kwamw Nkrumah univerisyt of Science and
fatty acids to humans in Ghana
Technology, Ghana
HC Principal Investigator
Budget Period: Year 2
Regina Edziyie From (mm/dd/yy): 10/2/2013
To (mm/dd/yy): 9/30/2014
Budget Categories
Personnel Costs
Host Country Cost
HC Contribution
Salary $ 7,200 $ 7,200
Benefits/Fringe $ 1,200 $ 300
Subtotal $ 8,400 $ 7,500
Supplies and Services
S& S $ 7,025
Subtotal $ 7,025
Travel
Domestic $ 1,000
International $ 10,700
Subtotal $ 11,700
Equipment
Tuition $ 2,000
Total Direct Cost $ 29,125
Indirect Costs $ 0
Standard Indirect $ 2,713
Subtotal $ 2,713
Total $ 31,838
100
$ 3,000
$ 10,500
$ 1,050
$ 11,550

BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title HC Institution
Enhancing Tilapia diets to improve source of n-3 Kwamw Nkrumah univerisyt of Science and
fatty acids to humans in Ghana
Technology, Ghana
HC Principal Investigator
Budget Period: Year 3
Regina Edziyie From (mm/dd/yy): 10/1/2014
To (mm/dd/yy): 9/30/2015
Budget Categories
Personnel Costs
Host Country Cost
HC Contribution
Salary $ 7,200 $ 7,200
Benefits/Fringe $ 1,200 $ 300
Subtotal $ 8,400 $ 7,500
Supplies and Services
S& S $ 5,200
Subtotal $ 5,200
Travel
Domestic $ 1,100
International $ -
Subtotal $ 1,100
Equipment
Tuition $ 2,000
Total Direct Cost $ 16,700
Indirect Costs
Standard Indirect $ 1,470
Subtotal $ 1,470
Total $ 18,170
101
$ 3,000
$ 10,500
$ 1,050
$ 11,550

BUDGET FORM
AquaFish Innovation Lab
Combined-Year HOST COUNTRY I nstitution Budget
Proposal Title HC Institution
Enhancing Tilapia diets to improve source of n-3 fatty Kwamw Nkrumah univerisyt of Science and
acids to humans in Ghana
Technology, Ghana
HC Principal Investigator
Budget Period: Combined Years
Regina Edziyie From (mm/dd/yy): 7/1/2013
To (mm/dd/yy): 9/30/2015
Budget Categories
Personnel Costs
Host Country Cost
HC Contribution
Salary $ 19,200 $ 21,600
Benefits/Fringe $ 3,000 $ 900
Subtotal $ 22,200 $ 22,500
Supplies and Services
S& S $ 14,605
Subtotal $ 14,605
Travel
Domestic $ 2,950
International $ 10,700
Subtotal $ 13,650
Equipment $ -
Tuition $ 4,500
Total Direct Cost $ 54,955
Indirect Costs
Standard Indirect $ 5,046
Subtotal $ 5,046
Total $ 60,001
102
$ 6,000
$ 6,000
$ 28,500
$ 2,850
$ 31,350

Specify Year 1 (3 months)
BUDGET JUSTIFICATION FORM FOR HOST COUNTRY INSTITUTIONS
KWAME NKRUMAH UNIVERSITY OF SCIENCE AND TECHNOLOGY (KNUST)
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Regina Esi
Edziyie
Nelson Winston
Agbo
Collins Duodo
TBD*
HC PI $24,00
0
HC
$30,00
Investigator
0
Graduate
student
$4800
Research $1200
Associate
0
Benefits
Rate
Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefit
s
25% 0.05 1200 300 0.05 $1200 300
25% 0.04 1200 300
103
0.5 2400 -
- - - - 0.5 $6000 -
Total 4800 600 7200 300
* National service scheme personnel as research assistants
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
Collins Duodo Male Ghanaian M.Phil Aquaculture
-
-
1c. Matching Contributions
5% of PI salary and 50% of a Research Associate salary with fringe plus indirect cost valued at $8250 is used as
match for first year.
2. Services and Supplies
Cost of locally available n-3 materials and control fish -- $780
Cost of packaging and shipping materials and control fish to UAPB for analysis -- $1200
Communication -- $400
Total -- 2380
3. Travel
Domestic Travel
Date: August -September 2013 Name: Regina Edziyie and Collins Duodo Reason for
travel: Purchase of local sources from different markets around the country and Shipment of
materials
Departure Location: Kumasi Arrival Location: Kumasi (for shipment) ,
Variable ( for local n-3 materials)
Meals and Lodging @ $30/day for 10 days $ 300
Local transportation $ 550
TOTAL $ 850
International Travel
4. Tuition
Tuition $500
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
10% both $863

6. HC/Regional Cost $9,993
104

Specify Year 2
BUDGET JUSTIFICATION FORM FOR HOST COUNTRY INSTITUTIONS
KWAME NKRUMAH UNIVERSITY OF SCIENCE AND TECHNOLOGY (KNUST)
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Benefits
Rate
105
Project Funds Cost Share Funds
FTE Salary Benefits FTE Salar
y
Benefits
Regina Esi
Edziyie
HC PI $24,000 25% 0.1 2400 600 0.05 $1200 300
Nelson Investigato $30,000 25% 0.08 2400 600
Winston
Agbo
r
Collins Graduate $4800 0.5 2400 -
Duodo student
TBD* Research
Associate
$12000 - - - - 0.5 $6000 -
Total $7200 1200 $7200 300
* National service scheme personnel as research assistants
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
Collins Duodo Male Ghanaian M.Phil Aquaculture
1c. Matching Contributions
5% of PI salary and 50% of a Research Associate salary with fringe valued at $7500 with fringe; use of ponds /
harpers and other on-farm facilities including cost of pumping water valued at $3000. The total match for Year 2
with indirect is $11,550.
2. Services and Supplies
Cost of fingerlings and feed -- $3000
Cost of servicing water quality equipment and reagents -- $3000
Harper repair -- $25
Communication -- $800
Publication -- $200
Total -- 7025
3. Travel
Domestic Travel
Date: 2013 Name: Regina Edziyie and Collins Duodo Reason for travel:
Purchase of fingerlings,
Departure Location: Kumasi Arrival Location: Akosombo (fingerlings) ,
Accra (feed)
Meals and Lodging @ $200 /day for 3 days total $ 600
Local transportation ($250 for live transport of fish) $ 400
TOTAL $ 1000
International Travel

Date: Febraury 2015 Names: Nelson W. Agbo / Regina Edziyie
Reason for travel: Presentation of partial results and participation in Aquafish Annual
Meeting at Seattle 2015
Departure Location: Accra Arrival Location: Seattle
Meals and Lodging @ $200/day for 5 days X 2 $2000
Airfare X 2 $5000
Airport transport, parking, etc. $100
Miscellaneous (departure tax, etc.) $100
Sub-total $ 7200
Date: September 2013 Name: Collins Duodo
Reason for travel: Fatty acid analysis and training at UAPB
Departure Location: Accra Arrival Location: Little Rock
Meals and Lodging @ $100/day for 13.5 days $ 1350
Airfare $2000
Airport transport, parking, etc. $50
Miscellaneous (departure tax, etc.) $100
Sub-total $ 3500
TOTAL $10,700
4. Tuition
Tuition $2000
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
0.10 both $2713
6. HC/Regional Cost $31,838
106

Specify Year 3
BUDGET JUSTIFICATION FORM FOR HOST COUNTRY INSTITUTIONS
KWAME NKRUMAH UNIVERSITY OF SCIENCE AND TECHNOLOGY (KNUST)
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Benefits
Rate
107
Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefit
s
Regina Esi
Edziyie
HC PI $24,000 25% 0.1 2400 600 0.05 $1200 300
Nelson Investigato $30,000 25% 0.08 2400 600
Winston Agbo r
Collins Duodo Graduate
student
$4800 0.5 2400 -
TBD* Research
Associate
$12000 - - - - 0.50 $6000 -
Total $7200 1200 $7200 300
* National service scheme personnel as research assistants
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
Collins Duodo Male Ghanaian M.Phil Aquaculture
1c. Matching Contributions
5% of PI salary and 50% of a Research Associate salary with fringe valued at $7500 with fringe; use of ponds /
harpers and other on-farm facilities including cost of pumping water valued at $3000. The total match for Year 2
with indirect is $11,550.
2. Services and Supplies
Cost of fingerlings and feed -- $3250
Cost of servicing water quality equipment and reagents -- $200
Replacement of harpers -- $500
Pond maintenance and walkway repair -- $150
Communication -- $800
Publication -- $300
Total -- 5200
3. Travel
Domestic Travel
Date: 2013 Name: Nelson Agbo and Collins Duodo
Reason for travel: Purchase of fingerlings, transport feed
Departure Location: Kumasi Arrival Location: Akosombo (fingerlings) ,
Accra (feed)
Meals and Lodging @ $200 /day for 3 days total $ 600
Local transportation ($250 for live transport of fish) $ 500
TOTAL $ 1100

4. Tuition
Tuition $2000
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
0.10 both $1470
6. HC/Regional Cost $18,170
108

UHH Sub to WIOMSA
Proposal Title HC Institution
Institute of Marine Sciences, University of Dar es
Coastal Women’s Shellfish Aquaculture Development Salaam
Year 1, Year 2, or Year 3
HC Principal Investigator
Budget Period:
6/30/2009
(select one)
Narriman Jiddawi
To (9/30/13): Year 1
Budget Categories Host Country Cost
HC Contribution
Personnel Costs
Salary $ 2,850
Benefits/Fringe $ 524
Subtotal $ 3,374
Supplies and Services
S&S $ 500
Subtotal $ 500
Travel
Domestic
International
Subtotal $ -
Equipment $ -
Tuition $ -
Total Direct Cost $ 3,874
Indirect Costs
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Standard Indirect $ 542
Subtotal $ 542
Total $ 4,416
109
$ -
$ -
$ -
$ -
$ -
$ -
$ -
$ -

Proposal Title HC Institution
Institute of Marine Sciences, University of Dar es
Coastal Women’s Shellfish Aquaculture Development Salaam
Year 1, Year 2, or Year 3
HC Principal Investigator
Budget Period:
9/30/2009
(select one)
Narriman Jiddawi
To (9/30/14): Year 2
Budget Categories Host Country Cost
HC Contribution
Personnel Costs
Salary $ 14,550
Benefits/Fringe $ 3,075
Subtotal $ 17,625
Supplies and Services
S& S $ 17,365
Subtotal $ 17,365
Travel
Domestic $ 8,700
International $ 9,000
Subtotal $ 17,700
Equipment $ -
Tuition $ 500
Total Direct Cost $ 53,190 $ 21,687
Indirect Costs
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Standard Indirect $ 7,447 $ 3,036
Subtotal $ 7,447 $ 3,036
Total $ 60,637
110
$ 7,750
$ 1,937
$ 9,687
$ 12,000
$ 12,000
$ -
$ -
$ -
$ 24,723

Proposal Title HC Institution
Institute of Marine Sciences, University of Dar
Coastal Women’s Shellfish Aquaculture Development es Salaam
HC Principal Investigator
Budget Period: Combined Years
Narriman Jiddawi
From (7/1/13): YR1-YR2
To (9/30/14):
Budget Categories Host Country Cost
Personnel Costs
Salary $ 17,400
Benefits/Fringe $ 3,599
Subtotal $ 20,999
Supplies and Services
S& S $ 17,865
Subtotal $ 17,865
Travel
Domestic $ 8,700
International $ 9,000
Subtotal $ 17,700
Equipment $ -
Tuition $ 500
Total Direct Cost $ 57,064
Indirect Costs
BUDGET FORM
AquaFish Innovation Lab
Combined-Year HOST COUNTRY Institution Budget
Standard Indirect $ 7,989
Subtotal $ 7,989
Total $ 65,053
111
HC Contribution
$ 7,750
$ 1,937
$ 9,687
$ 12,000
$ 12,000
$ 21,687
$ 3,036
$ 3,036
$ 24,723

Year 1 WIOMSA
AQUAFISH INNOVATION LAB
BUDGET JUSTIFICATION INSTRUCTIONS AND FORM
FOR HOST COUNTRY INSTITUTIONS - WIOMSA
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Benefits Rate Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Narriman Professor 33,600 25% 0.12 2100 525
Jiddawe
5
TBA Graduate 6,000 25% 0.12 750 0
student
5
Total 2850 525
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
TBA Female Tanzanian M.S.
1c. Matching Contributions
All required matching is provided in the Year 2 budget.
2. Services and Supplies
Fund requested include $500 to purchase spat collection materials (rope, line, shade cloth).
3. Travel
Domestic Travel
Date Name Reason for travel:
Departure Location: Arrival Location:
Meals and Lodging @ $ /day for days $
Airfare and ground transportation $
Registration $
TOTAL $
International Travel
Date Name Reason for travel:
Departure Location: Arrival Location:
Meals and Lodging @ $ /day for days $
Airfare $
Airport transport, parking, etc. $
Miscellaneous (departure tax, etc.) $
TOTAL $
4. Tuition
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
14% of $3,874 of direct costs On-campus $542
6. HC/Regional Cost
All costs are HC costs.
112

Year 2 WIOMSA
AQUAFISH INNOVATION LAB
BUDGET JUSTIFICATION INSTRUCTIONS AND FORM
FOR HOST COUNTRY INSTITUTIONS - WIOMSA
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Benefits Rate Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Narriman Professor 33,600 25% 0.10 6300 1575 0.104 1750 438
Jiddawe
4
TBA Graduate
student
6,000 25% 0.25 2250 0.25 3000 750
TBA Project 12,000 25% 0.37 6000 1500 0.25 3000 750
Assistant
5
Total 14550 3075 7750 1938
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
TBA Female Tanzanian M.S.
1c. Matching Contributions ($24,723)
Matching contributions are:
1.25 months salary equivalent from Dr. Narriman Jiddawe for $1750 plus 25% fringe benefits.
3 months salary equivalent from the Project Assistant for $3000 plus 25% fringe benefits.
6 months salary equivalent from the graduate student salary for $3000 plus 25% fringe benefits.
Matching contributions from salaries and fringe total $9688.
WIOMSA will provide meeting and working space for project personnel and community members for
$1000 per month for twelve months for a total of $12000.
Total direct match costs are $21688. Indirect costs on this (@14%) are $3036. Total match is therefore 24,
723.
2. Services and Supplies ($17,365)
Fund requested include:
Materials for shellfish culture and spat collection trials: $5080
Conference facilities fees (45 persons, $35/person/day, for two 2 day workshops): $6300
Workshop materials, $350 per workshop for two workshops: $700
Quarterly meetings (4 per year) in 2 villages, conference fees $300, materials $75: $3000
Fees for facilitators (2 people), $100 per day/person, 2 workshops: $400
Office supplies: $1885
3. Travel ($17,700)
Domestic Travel
Date: throughout the year
Name: Narriman Jiddawe or Project Assistant
Reason for travel: extension, this is three trips per month for a total of 36 visits per year
Departure Location: Zanzibar
Arrival Location: Zanzibar villages
Meals and Lodging @ $ 50/day for 36 days $1800
Airfare and ground transportation @$50 per trip $1800
Registration $
113

TOTAL $3600
Date through out the year Name Graduate student
Reason for travel: extension each trip will be a one week stay at a village, for 15 trips
Departure Location: Zanzibar
Arrival Location: Zanzibar villages
Meals and Lodging @ $ 50 /week for 15 weeks $ 750
Airfare and ground transportation, @$50/trip $ 750
Registration $
TOTAL $1500
Date: TBA Name: Workshop participants (45 people)
Reason for travel: attend women’s workshop and village meetings (2 day meetings)
Departure Location: Zanzibar
Arrival Location: Zanzibar
Meals and Lodging @ $ 20 /day for 2 days, 45 people $1800
Airfare and ground transportation: $40/person, 45 persons $1800
Registration $
TOTAL $3600
International Travel
Date: March 2014
Name Narriman Jiddawe and Graduate student
Reason for travel: present research findings at WAS meeting
Departure Location: Zanzibar
Arrival Location: Seattle, WA
Meals and Lodging @ $250 /day for 6 days, 2 people $3000
Airfare, 2 pax @$3000 each $6000
Airport transport, parking, etc. $
Miscellaneous (departure tax, etc.) $
TOTAL $9000
4. Tuition
$500 for graduate student
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
14% of $53,190 of direct costs On-campus $7,447
6. HC/Regional Cost
All costs are HC costs.
114

VT Sub to KNUST
BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title HC Institution
Selecting better-adapted strains of native species
and evaluating Ghana's aquaculture development
plan for the changing climate
HC Principal Investigator
Budget Period: Year 1
Steve Amisah From (mm/dd/yy): 7/1/2013
To (mm/dd/yy): 9/30/2013
Budget Categories
Personnel Costs
Host Country Cost
HC Contribution
Salary $ 5,350 $ 5,830
Benefits/Fringe $ - $ -
Subtotal $ 5,350 $ 5,830
Supplies and Services
S& S $ 8,150
Subtotal $ 8,150
Travel
Domestic $ 1,500
International $ -
Subtotal $ 1,500
Equipment $ -
Tuition $ -
Total Direct Cost $ 15,000
Indirect Costs $ -
Standard Indirect $ -
Subtotal $ -
Total $ 15,000
Kwamw Nkrumah University of Science and
Technology, Ghana
115
$ 5,830
$ -
$ -
$ -
$ 5,830

BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title HC Institution
Selecting better-adapted strains of native species
and evaluating Ghana's aquaculture development
plan for the changing climate
HC Principal Investigator
Budget Period: Year 2
Steve Amisah From (mm/dd/yy): 10/1/2013
To (mm/dd/yy): 9/30/2014
Budget Categories
Personnel Costs
Host Country Cost
HC Contribution
Salary $ 13,690 $ 11,150
Benefits/Fringe $ - $ -
Subtotal $ 13,690 $ 11,150
Supplies and Services
S& S $ 23,910
Subtotal $ 23,910
Travel
Domestic $ 4,000
International $ 3,900
Subtotal $ 7,900
Equipment
Tuition $ 2,000
Total Direct Cost $ 47,500
Indirect Costs $ -
Standard Indirect $ -
Subtotal $ -
Total $ 47,500
Kwamw Nkrumah University of Science and
Technology, Ghana
116
$ -
$ 11,150
$ 1,115
$ 12,265

BUDGET FORM
AquaFish Innovation Lab
Single-Year HOST COUNTRY I nstitution Budget
Proposal Title HC Institution
Selecting better-adapted strains of native species
and evaluating Ghana's aquaculture development
plan for the changing climate
HC Principal Investigator
Budget Period: Year 3
Steve Amisah From (mm/dd/yy): 10/1/2014
To (mm/dd/yy): 9/30/2015
Budget Categories
Personnel Costs
Host Country Cost
HC Contribution
Salary $ 10,490 $ 2,620
Benefits/Fringe $ - $ -
Subtotal $ 10,490 $ 2,620
Supplies and Services
S& S $ 18,010
Subtotal $ 18,010
Travel
Domestic $ 2,500
International $ 3,500
Subtotal $ 6,000
Equipment
Tuition $ 1,000
Total Direct Cost $ 35,500
Indirect Costs
Standard Indirect $ -
Subtotal $ -
Total $ 35,500
Kwamw Nkrumah University of Science and
Technology, Ghana
117
$ -
$ 2,620
$ -
$ 2,620

BUDGET FORM
AquaFish Innovation Lab
Combined-Year HOST COUNTRY I nstitution Budget
Proposal Title HC Institution
Selecting better adapted strains of native species and
evaluating Ghana's aquaculture development plan for
the changing climate
HC Principal Investigator
Budget Period: Combined Years
Steve Amisah From (mm/dd/yy): 7/1/2013
To (mm/dd/yy): 9/30/2015
Budget Categories
Personnel Costs
Host Country Cost
HC Contribution
Salary $ 29,530 $ 19,600
Benefits/Fringe $ - $ -
Subtotal $ 29,530 $ 19,600
Supplies and Services
S& S $ 50,070
Subtotal $ 50,070
Travel
Domestic $ 8,000
International $ 7,400
Subtotal $ 15,400
Equipment $ -
Tuition $ 3,000
Total Direct Cost $ 98,000
Indirect Costs
Standard Indirect $ -
Subtotal $ -
Total $ 98,000
118
Kwamw Nkrumah University of Science and
Technology, Ghana
$ -
$ -
$ 19,600
$ -
$ 19,600

Specify Year 1 (3 months)
BUDGET JUSTIFICATION FORM FOR HOST COUNTRY INSTITUTIONS
KWAME NKRUMAH UNIVERSITY OF SCIENCE AND TECHNOLOGY (KNUST)
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Benefits
Rate
119
Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Steve Amisah HC PI $47,000 n/a 0.05 2,350 0 0.05 2,350 0
Gifty Anane-
Taabeah
HC
Investigator
$20,000
n/a 0.10 2,000 0 0.10 2,000 0
TBD
Graduate
student
$4,800
n/a 0.00 0 0 0.10 480 0
TBD
Research
Assistant
$10,000
n/a 0.10 1,000 0 0.10 1,000 0
Total $5,350 0 $5,830 0
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
TDB TDB Ghanaian MPhil Aquaculture
-
1c. Matching Contributions
5% of HC PI salary, 10% each of HC co-PI/Investigator, Research Assistant, and Graduate Student stipends
valued at $5830 is used as match for year 1.
2. Services and Supplies
First Evaluation Workshop:
Facility rental for 3 days $ 600
Hostel lodging for 30 participants and resource persons for 3 nights $ 3,600
Meals for workshop participants $ 1,890
Transportation for participants $ 1,560
Miscellaneous workshop supplies $ 500
Total -- $ 8,150
3. Travel
Domestic Travel:
$1,500 is budgeted for domestic travel of project personnel for field reconnaissance surveys to identify
tributaries of the Volta Lake with thriving populations of O. niloticus and select field site. Cost covers
university vehicle rentals and fuel for 1-2 weeks.
International Travel:
4. Tuition
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
0 both 0
6. HC/Regional Cost $15,000

Specify Year 2
BUDGET JUSTIFICATION FORM FOR HOST COUNTRY INSTITUTIONS
KWAME NKRUMAH UNIVERSITY OF SCIENCE AND TECHNOLOGY (KNUST)
1. Personnel, Benefits, and Cost Share
Name Title Annual
Salary
1a. Salary Information
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
TDB TDB Ghanaian MPhil Aquaculture
-
1c. Matching Contributions
7% of HC PI salary, 10% each of HC co-PI/Investigator salary, 50% Graduate Student time, and 20% Research
Assistant time valued at $11,150 is used as match for year 2.
2. Services and Supplies
Materials and Supplies for Field Studies
Maintenance of water quality meters and reagent refills --$1,000
Field gear (e.g., seine) replacement and maintenance --$1,200
Field stationery, storage jars, etc. -- $ 500
Materials and Supplies for Lab Experiments and Laboratory works
Fingerlings of the Volta strain of O. niloticus from commercial hatchery --$1,500
Feed --$1,500
Installation of additional glass tanks, plumbing retrofitting, and maintenance --$4,000
Backup power generator rental and fuel for lab experiments --$2,500
Aerators, Aquarium pumps, Heater/Chillers --$4,500
Formalin, Alcohol, and other expendable supplies --$ 800
Second Evaluation & Training Workshop:
Facility rental for 3 days $ 600
Hostel lodging for 30 participants and resource persons for 3 nights $ 3,600
Meals for workshop participants $ 1,890
Transportation for participants $ 1,560
Miscellaneous workshop supplies $ 500
Total -- $ 8,150
TOTAL $23,910
3. Travel
Domestic Travel:
Benefit
s Rate
120
Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Steve Amisah HC PI $47,000 n/a 0.07 3,290 0 0.05 2,350 0
Gifty Anane-
Taabeah
HC
Investigat
or
$20,000
n/a 0.15 3,000 0 0.10 2,000 0
TBD
Graduate
student
$4,800
n/a 0.50 2,400 0 0.50 4,800 0
TBD
Research
Assistant
$10,000
n/a 0.50 5,000 0 0.20 2,000 0
Total $13,690 0 $11,150 0

$4,000 is budgeted for recurring domestic travels of project personnel for field sampling at sites in the
Volta basin. Cost covers university vehicle rentals and fuel. Additional recurring travels by public
transportation will be undertaken by personnel to conduct desk studies at regional offices.
International Travel:
Date: February 2014 Name: (Steve Amisah or Gifty Anane-Taabeah)
Reason for travel: Presentation of research and participation in Aquafish program Meeting at
WAS, Seattle
Departure Location: Accra Arrival Location: Seattle, WA
Registration $ 300
Meals and Lodging @ $200/day for 6 days $1200
Airfare $2100
Airport transport, parking, etc. $ 200
Miscellaneous (departure tax, etc.) $ 100
Sub-total $3,900
TOTAL $7,900
4. Tuition
$2,000
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
0 both 0
6. HC/Regional Cost $47,500
121

Specify Year 3
BUDGET JUSTIFICATION FORM FOR HOST COUNTRY INSTITUTIONS
KWAME NKRUMAH UNIVERSITY OF SCIENCE AND TECHNOLOGY (KNUST)
1. Personnel, Benefits, and Cost Share
1a. Salary Information
Name Title Annual
Salary
Benefits
Rate
122
Project Funds Cost Share Funds
FTE Salary Benefits FTE Salary Benefits
Steve Amisah HC PI $47,000 n/a 0.07 3,290 0 0.04 1,180 0
Gifty Anane-
Taabeah
HC
Investigator
$20,000
n/a 0.15 3,000 0 0.037 740 0
TBD
Graduate
student
$4,800
n/a 0.25 1,200 0 0.00 0 0
TBD
Research
Assistant
$10,000
n/a 0.30 3,000 0 0.00 0 0
Total $10,490 0 $2,620 0
1b. Student Information (you will be asked to complete a student profile form upon award)
Name Gender Nationality Degree Sought
TDB TDB Ghanaian MPhil Aquaculture
-
1c. Matching Contributions
4% of HC PI salary, and 3.7% of HC co-PI/Investigator salary valued at $2,620 is used as match for year 3.
2. Services and Supplies
Materials and Supplies for Field Studies
Maintenance of water quality meters and reagent refills --$ 300
Field gear (e.g., seine) replacement and maintenance --$ 300
Materials and Supplies for Lab Experiments and Laboratory works
Fingerlings of the Volta strain of O. niloticus from commercial hatchery --$1,000
Feed --$1,000
Lab Installation maintenance --$2,000
Backup power generator rental and fuel for lab experiments --$1,000
Formalin, Alcohol, and other expendable supplies --$ 410
Genetic Analysis --$ 5,000
Third Evaluation & Training Workshop:
Facility rental for 3 days $ 600
Hostel lodging for 20 participants and resource persons for 3 nights $ 2,600
Meals for workshop participants $ 1,790
Transportation for participants $ 1,510
Miscellaneous workshop supplies $ 500
Total -- $ 7,000
TOTAL $18,010
3. Travel
Domestic Travel:
$2,500 is budgeted for recurring domestic travels of project personnel for field sampling at sites in the
Volta basin. Cost covers university vehicle rentals and fuel. Additional recurring travels by public
transportation will be undertaken by personnel to conduct desk studies at regional offices.

International Travel:
Date: February 2015 Name: (Steve Amisah or Gifty Anane-Taabeah)
Reason for travel: Presentation of research at WAS, New Orleans
Departure Location: Accra Arrival Location: New Orleans, Louisiana
Registration $ 300
Meals and Lodging @ $200/day for 6 days $ 900
Airfare $2100
Airport transport, parking, etc. $ 100
Miscellaneous (departure tax, etc.) $ 100
Sub-total $3,500
TOTAL $6,000
4. Tuition
$1,000
5. Indirect Costs
Rate of Regular Indirect On-/Off-campus Amount
0 both 0
6. HC/Regional Cost $35,500
123

AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
LEVERAGED SUPPORT
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT
The following information must be submitted: Type of leveraged funding (travel, external grant from
another agency, USAID buy-in from a Mission, University support in excess of committed cost share
or match), amount, source, other details as are available. Use additional sheets as necessary.
ANTICIPATED LEVERAGING FROM PROPOSED WORK
The following information must be submitted: Leveraged funding anticipated for the next two years.
Use additional sheets as necessary.
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title
and Lead PI:
PI submitting this form:
Other Agencies this proposal has
been/will be submitted to:
Aquaculture Production and Human Health, Nutrition, and Food Supply in
Ghana and Tanzania
Kwamena Quagrainie
124
Kwamena Quagrainie
None

Current/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title:
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: NOAA
Total Award Amount: 1,244,564 Total Award Period: 02/01/2010 -01/31/2014
Location of Project:
Illinois & Indiana, USA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
33%
Project Title:
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: NOAA
Total Award Amount: 219,259 Total Award Period: 10/01/2010 -01/31/2014
Location of Project:
Illinois & Indiana, USA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
2%
Project Title:
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: USAID
Total Award Amount: 194,822 Total Award Period: 06/03/2011 -09/30/2013
Location of Project:
Ghana, Kenya & Tanzania
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
2%
Project Title:
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: USDA
Total Award Amount: 4,000 Total Award Period: 09/01/2011 -08/31/2013
Location of Project:
Indiana, USA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
2%
125

Project Title:
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: Indiana Soybean Alliance
Total Award Amount: 5,290 Total Award Period: 06/01/2012 -05/31/2013
Location of Project:
Indiana, USA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
2%
Project Title:
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: USDA
Total Award Amount: 2,150 Total Award Period: 09/01/2013 - 08/31/2015
Location of Project:
Indiana, USA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
2%
Project Title:
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: NOAA
Total Award Amount: 300,000 Total Award Period: 10/01/2013 - 09/30/2015
Location of Project:
Indiana & Kentucky, USA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
2%
126

AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
LEVERAGED SUPPORT
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT
The following information must be submitted: Type of leveraged funding (travel, external grant from
another agency, USAID buy-in from a Mission, University support in excess of committed cost share
or match), amount, source, other details as are available. Use additional sheets as necessary.
Research Associate supported on non-CRSP funds (USDA Evans-Allen) helped support CRSP
studies at UAPB ($5000).
ANTICIPATED LEVERAGING FROM PROPOSED WORK
The following information must be submitted: Leveraged funding anticipated for the next two years.
Use additional sheets as necessary.
Additional travel funding from state funds (up to $2000) will be used to supplement CRSP funds.
Research Associate and Grad. Student supported on non-CRSP funds (USDA Evans Allen, AR corn
and sorghum board - up to $7000) will help support CRSP studies at UAPB.
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title
and Lead PI:
PI submitting this form:
Other Agencies this proposal has
been/will be submitted to:
Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania (Kwamena Quagrainie)
None
127
Rebecca Lochmann

Current/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title: Optimizing the finishing period and dietary inclusion level of CLA-soybean oil to produce CLA-enhanced channel
catfish as a functional food,
Project Status: ( X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: Arkansas Soybean Promotion Board
Total Award Amount: $26,400 Total Award Period: 04/01/2012 – 03/31/2013
Location of Project: UAPB
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
5%
Project Title: Minimizing use of fishmeal in hybrid striped bass (HSB) diets using non-GMO soybeans selectively bred for use in
aquafeeds.
Project Status: (X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: Arkansas Soybean Promotion Board
Total Award Amount: $34,153 Total Award Period: 04/01/2012 – 03/31/2013
Location of Project: UAPB and SNARC
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
5% each, 2 co-PIs
Project Title: Improving Catfish Broodstock Management by Manipulating Diet, Stocking Densities and Sex Ratios
Project Status: (X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: USDA Southern Regional Aquaculture Center
Total Award Amount: $128,385 Total Award Period: 01/01/2012 – 12/31/2014
Location of Project: UAPB, TAMU, Mississippi State
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
5 - 10% per co-PI.
____________________________________________________________________________________________________________
Project Title: The Effects of Diets with Corn Distillers Dried Grains or Sorghum Distillers Dried Grains on
Catfish Performance, Processing Traits, and Production Profitability
Project Status: (X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: Arkansas Corn and Grain Sorghum Promotion Board
Total Award Amount: $26,000 Total Award Period: April 1, 2013 - March 31, 2014
Location of Project: UAPB
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project): 5%
128

AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
MARIA HAWS
LEVERAGED SUPPORT
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT
Previous work conducted by UHH in Zanzibar received funding from the USAID SUCCESS
program.
ANTICIPATED LEVERAGING FROM PROPOSED WORK
None
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title Aquaculture Production and Human Health in Ghana and Tanzania
and Lead PI:
Kwamena Quagrainie
PI submitting this form:
Other Agencies this proposal has
been/will be submitted to:
none
129
Maria Haws

Current/Pending Research Projects – Maria Haws
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title: Expanding and Diversifying Near-shore Mariculture in Hawaii and the U.S.
Affiliated Pacific Islands Through Resolution of Regulatory, Technical and Biological
Impediments
Project Status: ( X) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: Sea Grant
Total Award Amount: $291,000 Total Award Period:10/12 to 8/14
Location of Project: Hawaii
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
0.08
Project Title: Establishing bivalve farming in Hawai`i.
Project Status: (X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: USDA
Total Award Amount: 75,000 Total Award Period: 10/12 to 8/14
Location of Project: Hawaii
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
0.05
03
Project Title:
Project Status: (X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency:
Total Award Amount: Total Award Period:
Location of Project: Hawaii
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Project Title: Human Health and Aquaculture. Human health and aquaculture: AquaFish
CRSP, OSU. Phase IV
Project Status: (X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: AquaFish CRSP
Total Award Amount: 450,000 Total Award Period: 1/12 to 1/13.
Location of Project: Mexico, Nicaragua
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
0.05
130

Project Title: Microalgae for Biofuels & Fish Feeds.
Project Status: (X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: USDA
Total Award Amount: $99,834 Total Award Period: 1/12 to 12/12
Location of Project: Hawaii
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
0.05
Project Title: Culturing native species of macroalgae in Hawai‘i and the U.S. Affiliated
Pacific Islands
Project Status: (X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: USDA
Total Award Amount: 36,030 Total Award Period: 10/11 to 9/13.
Location of Project: Hawaii and Micronesia
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
0.05
2613
131

AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
LEVERAGED SUPPORT
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT
The following information must be submitted: Type of leveraged funding (travel, external grant from
another agency, USAID buy-in from a Mission, University support in excess of committed cost share
or match), amount, source, other details as are available. Use additional sheets as necessary.
ANTICIPATED LEVERAGING FROM PROPOSED WORK
The following information must be submitted: Leveraged funding anticipated for the next two years.
Use additional sheets as necessary.
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title
and Lead PI
PI submitting this form:
Other Agencies this proposal has
been/will be submitted to:
Aquaculture Production and Human Health, Nutrition, and Food Supply in
Ghana and Tanzania (Kwamena Quagrainie)
132
Prof. Steve Amisah
Current/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title:
Title: Development of a M.Sc. curriculum that uses Problem Based Learning (PBL) and blended
learning approaches in teaching and learning to build capacity and link universities, aquaculture
community, industry and civil society
Project Status: ( ) Current ( X ) Pending ( ) Submission Planned in near Future
Funding Agency: DANIDA (Building Stronger Universities: BSU)
Total Award Amount: USD 104, 967 Total Award Period: Sept: 2013-2014
Location of Project:KNUST, Kumasi, GHANA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Prof. Steve Amisah FTE (Lead PI: 0.10)

Project Title: Strategic Research Cooperation Projects 2013, Phase 2
Enhancing Sustainable Tilapia Culture in Ghana; Value Chain
Project Status: ( x) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: DANIDA
Total Award Amount:USD: 1,800 Total Award Period: March-July 2013
Location of Project: Ghana, KNUST
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Prof. Steve Amisah Lead PI: FTE 0.05
032613
133

AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
LEVERAGED SUPPORT
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT
The following information must be submitted: Type of leveraged funding (travel, external grant from
another agency, USAID buy-in from a Mission, University support in excess of committed cost share
or match), amount, source, other details as are available. Use additional sheets as necessary.
Three Research Assistants supported on non-CRSP funds (Ghana Government) helped support CRSP
workshops and studies at KNUST ($12,000).
ANTICIPATED LEVERAGING FROM PROPOSED WORK
The following information must be submitted: Leveraged funding anticipated for the next two years.
Use additional sheets as necessary.
Two Research Assistants and supported on non-CRSP funds (Ghana Government) will help support
CRSP studies at KNUST (18,000).
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title
and Lead PI:
PI submitting this form:
Other Agencies this proposal has
been/will be submitted to:
Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania (Kwamena Quagrainie)
None
134
Regina Edziyie

Current/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title: Enhancing Sustainable Tilapia Culture in Ghana
Project Status: ( ) Current ( X ) Pending (Stage II) ( ) Submission Planned in near Future
Funding Agency: Danish International Development Agency (DANIDA)
Total Award Amount: $1,000,000 Total Award Period: 01/2014 - 08/2017
Location of Project: KNUST
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
4 Co-PIs, 5%
Project Title: Building capacity and linkages among aquaculture stakeholders in Ghana through problem based learning and
blended learning approaches
Project Status: ( ) Current ( X ) Pending (Stage II) ( ) Submission Planned in near Future
Funding Agency: DANIDA
Total Award Amount: $105,000 Total Award Period: 01/2014 - 08/2015
Location of Project: KNUST
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
4 Co-PIs , 5%
Project Title: Water quality monitoring for improved aquaculture productivity
Project Status: ( ) Current ( X ) Pending ( ) Submission Planned in near Future
Funding Agency: Danish International Development Agency (DANIDA)
Total Award Amount: $12,000 Total Award Period: 01/2013 - 08/2014
Location of Project: KNUST
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
5%
135

AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
LEVERAGED SUPPORT
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT
The following information must be submitted: Type of leveraged funding (travel, external grant from
another agency, USAID buy-in from a Mission, University support in excess of committed cost share
or match), amount, source, other details as are available. Use additional sheets as necessary.
Three Research Assistants supported on non-CRSP funds (Ghana Government) helped support CRSP
workshops and studies at KNUST ($12,000).
ANTICIPATED LEVERAGING FROM PROPOSED WORK
The following information must be submitted: Leveraged funding anticipated for the next two years.
Use additional sheets as necessary.
Two Research Assistants and supported on non-CRSP funds (Ghana Government) will help support
CRSP studies at KNUST (18,000).
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title
and Lead PI:
PI submitting this form:
Other Agencies this proposal has
been/will be submitted to:
Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania (Kwamena Quagrainie)
None
136
R Nelson Winston Agbo

Current/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title: Enhancing Sustainable Tilapia Culture in Ghana
Project Status: ( ) Current ( X ) Pending (Stage II) ( ) Submission Planned in near Future
Funding Agency: Danish International Development Agency (DANIDA)
Total Award Amount: $1,000,000 Total Award Period: 01/2014 - 08/2017
Location of Project: KNUST
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
4 Co-PIs, 5%
Project Title: Building capacity and linkages among aquaculture stakeholders in Ghana through problem based learning and
blended learning approaches
Project Status: ( ) Current ( X ) Pending (Stage II) ( ) Submission Planned in near Future
Funding Agency: DANIDA
Total Award Amount: $105,000 Total Award Period: 01/2014 - 08/2015
Location of Project: KNUST
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
4 Co-PIs , 5%
Project Title: Water quality monitoring for improved aquaculture productivity
Project Status: ( ) Current ( X ) Pending ( ) Submission Planned in near Future
Funding Agency: Danish International Development Agency (DANIDA)
Total Award Amount: $12,000 Total Award Period: 01/2013 - 08/2014
Location of Project: KNUST
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
5%
137

.
AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
LEVERAGED SUPPORT
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT: NONE
ANTICIPATED LEVERAGING FROM PROPOSED WORK: NONE
•
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
Lead PI: Kwamena Quagrainie
PI submitting this form: Sebastian Chenyambuga
Other Agencies this proposal has
been/will be submitted to:
None
138

Current/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency:
Total Award Amount: Total Award Period:
Location of Project:
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Project Title:
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency:
Total Award Amount: Total Award Period:
Location of Project:
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
032613
139

.
AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
LEVERAGED SUPPORT
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT: NONE
ANTICIPATED LEVERAGING FROM PROPOSED WORK: NONE
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
Lead PI: Kwamena Quagrainie
PI submitting this form: Alloysius Attah
Other Agencies this proposal has
been/will be submitted to:
None
Current/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency:
Total Award Amount: Total Award Period:
Location of Project:
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
140

Project Title:
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency:
Total Award Amount: Total Award Period:
Location of Project:
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
032613
141

.
AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
LEVERAGED SUPPORT
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT: NONE
ANTICIPATED LEVERAGING FROM PROPOSED WORK: NONE
•
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
Lead PI: Kwamena Quagrainie
Co-Host Country PI
submitting this form:
Other Agencies this proposal has
been/will be submitted to:
None
142
Nazael Amos Madalla

Current/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency:
Total Award Amount: Total Award Period:
Location of Project:
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Project Title:
Project Status: ( ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency:
Total Award Amount: Total Award Period:
Location of Project:
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
032613
143

AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
CURRENT/PENDING RESEARCH PROJECTS
Disclosure Information Provided for:
Proposal Title: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
Lead PI: Kwamena Quagrainie
Co-Host Country PI
submitting this form:
Other Agencies this proposal has
been/will be submitted to:
None
144
Narriman Jiddawe
Current/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title: Enhancing sustainability of bivalves in Zanzibar through bivalve culture.
Project Status: ( X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: MCNIGHT
Total Award Amount: $40,000 / year Total Award Period: 4 years
Location of Project: Dar es salaam and Zanzibar
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
To provide training to communities on entrepreneurship related to bivalve farming
Best practices in Hatchery management
Experts from Oyster creek company and Woodshole University-came to provide the necessary trainings
and techniques for managing the hatchery etc in Zanzibar
Project Title: Investigation of the retail trade in marine curios of Tanzania. Tanzania has been produced
Project Status: ( X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: MASMA/WIOMSA
Total Award Amount: $55000 FOR IMS Total Award Period: 3 years
Location of Project: ZANZIBAR , KENYA, MADAGASCAR
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Determine the types and amount of shells collected by women in in the community / Location of
Markets for these shells / Determine areas where collection is done including the status of the
habitats and provide recommendation for management

AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
LEVERAGED SUPPORT
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT
The following information must be submitted: Type of leveraged funding (travel, external grant from
another agency, USAID buy-in from a Mission, University support in excess of committed cost share
or match), amount, source, other details as are available. Use additional sheets as necessary.
Virginia Tech provided assistantships and tuition waivers for HC students in excess of
required cost-share (~20% in excess of required cost-share)
Yaw, Ansah – HC student received the Borlaug LEAP fellowship for $20,000 that supported
two travels to Ghana for field work, in-country transportation for field work, outreach
activities, and a computer purchase for dissertation research.
Gifty Anane-Taabeah received $10,000 in PEO International Women’s Fellowship that
supported academic fees, tuition, and course materials while working on her AquaFish CRSP
research
ANTICIPATED LEVERAGING FROM PROPOSED WORK
The following information must be submitted: Leveraged funding anticipated for the next two years.
Use additional sheets as necessary.
BIOGEOGRAPHIC DISTRIBUTION MODELING OF OREOCHROMIS NILOTICUS WILL BENEFIT FROM
USGS SPONSORED SPECIES DISTRIBUTION MODELING PROJECT BEING UNDERTAKEN IN DR.
EMMANUEL FRIMPONG’S LAB. ANTICIPATED BENEFITS TO PROPOSED PROJECT INCLUDE
ENHANCED DATA ACQUISITION CAPABILITIES, GIS STAFF TIME, AND ACCESS TO HIGH-
PERFORMANCE COMPUTING EQUIPMENT.
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title
and Lead PI:
PI submitting this form:
Other Agencies this proposal has
been/will be submitted to: None
Aquaculture Production and Human Health, Nutrition, and Food Supply in
Ghana and Tanzania, PI Kwamena K. Quagrainie
145
Emmanuel A. Frimpong

Current/Pending Research Projects
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title: Testing the effect of context on a nest association mutualism of stream fishes in a
comparative-advantage biological market framework
Project Status: ( x ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: National Science Foundation (Division of Environmental Biology)
Total Award Amount: $206,549 Total Award Period: 2 years (5 months remaining)
Location of Project: Virginia, USA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Emmanuel Frimpong is the only PI; FTE committed is 0.11/year
Project Title: Data consolidation and development of an improved web-accessible public database on
traits and distribution of North American freshwater fishes
Project Status: ( x ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: US Geological Survey (Biological Informatics Division)
Total Award Amount: $197,103 Total Award Period: 2 years (3 months remaining)
Location of Project: Virginia, USA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Emmanuel Frimpong is the only PI; FTE committed is 0.11/year
Project Title: Preliminary application of a framework for modeling habitat suitability and distribution
of stream fishes with field-testing
Project Status: ( x ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: US Geological Survey (Core Science & Analytics Division – Aquatic GAP program)
Total Award Amount: $50,400 Total Award Period: 1 year (2 months remaining)
Location of Project: Virginia, USA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Emmanuel Frimpong is the only PI; FTE committed is 0.05/year
Project Title: Development of assessment tools for evaluating stream and watershed responses to
climate change along thermal gradients
Project Status: ( x ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: USDA Forest Service
Total Award Amount: $70,000 Total Award Period: 3 years (5 months remaining)
Location of Project: Virginia & Appalachian Mountain Regions, USA
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Paul Angermeier (Lead) & Emmanuel Frimpong are co-PIs; Frimpong FTE committed is 0.11/year
146

Project Title: Refining stream fish distribution models regionally for a nationally-consistent aquatic
gap analysis
Project Status: ( ) Current ( x ) Pending (invited) ( ) Submission Planned in near Future
Funding Agency: US Geological Survey (Core Science & Analytics – Aquatic GAP program)
Total Award Amount: $167,000 Total Award Period: 2 years
Location of Project: Virginia (Study area is conterminous USA)
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Emmanuel Frimpong is the only PI; FTE committed is 0.11/year
Project Title: Data consolidation and development of an improved web-accessible public database on
traits and distribution of North American freshwater fishes
Project Status: ( ) Current ( x ) Pending (invited continuation) ( ) Submission Planned in near Future
Funding Agency: US Geological Survey (Core Science & Analytics – Aquatic GAP program)
Total Award Amount: $61,000 Total Award Period: 1 year
Location of Project:
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
Emmanuel Frimpong (Lead) & Zhiwu Xie are co-PIs; Frimpong FTE committed is 0.04/year
Project Title: Climate change in arid to wet African and Asian delta ecosystems: water to policy –
planning for communities’ mitigating impacts on agriculture, fisheries, and health
Project Status: ( ) Current ( x ) Pending (concept note submitted) ( ) Submission Planned in near Future
Funding Agency: Collaborative Adaptation Research Initiative in Africa and Asia (CARIAA, Canada)
Total Award Amount: CAN$ 15,000,000 Total Award Period: 6 years
Location of Project: Ghana, Botswana, & Bangladesh
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
FTE Commitments not yet determined. This is a multi-stage application currently at the concept
note screening stage. Virginia Tech is lead consortium institution with co-PIs Emmanuel
Frimpong, Kathleen Alexander, and George Norton. McGill University (John Gyakum - PI) is
collaborating.
Project Title: Making markets work for the poor in the Nepal aquaculture sector
Project Status: ( ) Current ( x ) Pending ( ) Submission Planned in near Future
Funding Agency: SAMARTH – UKAID
Total Award Amount: $ 720,347 Total Award Period: 2 years
Location of Project: Nepal
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
FTE Commitments not yet determined. This application was submitted in collaboration with
DEVTEC Nepal. Virginia Tech PIs are Emmanuel Frimpong, Eric Hallerman, George Norton,
and Tom Hammet. Purdue University (Kwamena Quagrainie – PI) is collaborating.
032613
147

AQUAFISH INNOVATION LAB
PROPOSAL LEVERAGING STATEMENT AND PENDING FUNDS DISCLOSURE FORM
LEVERAGING ACTIVITIES TO DATE UNDER YOUR CURRENT PROJECT
Previous work conducted by CRC, UHH, IMS and WIOMSA in Zanzibar received funding from the
USAID SUCCESS program.
ANTICIPATED LEVERAGING FROM PROPOSED WORK
None
•
PENDING FUNDS
ADDITIONAL RESEARCH ACTIVITIES
The following information must be submitted by each US and HC Principal Investigator mentioned in
the proposal. Use additional sheets as necessary.
Disclosure Information Provided for:
Proposal Title Aquaculture Production and Human Health in Ghana and Tanzania
and Lead PI:
Kwamena Quagrainie
PI submitting this form:
Other Agencies this proposal has
been/will be submitted to:
None
148
JULIUS FRANCIS
Current/Pending Research Projects – Julius Francis
Please provide this information for each research project currently supported through non-AquaFish
funds as well as for each project that has been or will be submitted in the near future for non-
AquaFish funding.
Project Title: Sustainable Coastal Communities and Ecosystems (SUCCESS) Programme
Project Status: ( X) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: USAID
Total Award Amount: $ 300 000 Total Award Period:2009 to 2014
Location of Project: Western Indian Ocean region
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
0.08

Project Title: PWANI Project
Project Status: (X ) Current ( ) Pending ( ) Submission Planned in near Future
Funding Agency: USAID
Total Award Amount: 230 000 Total Award Period: 2011 to 2014
Location of Project: Zanzibar
FTE Committed to the Project (Please name and detail the FTE commitment of each Co-PI on this project):
0.05
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159

Kwamena K. Quagrainie
Purdue University, West Lafayette, IN 47907-2056
Tel: (765) 494 4200 / Fax: (765) 494 9176
Email: kquagrai@purdue.edu
EDUCATION
Ph.D. in Agricultural Economics, 2000, University of Alberta, Edmonton, Canada
MSc. in Agricultural Economics, 1995, University of Alberta, Edmonton, Canada
BSc. in Agriculture, 1982, University of Science and Technology, Ghana
PROFESSIONAL EMPLOYMENT
2005 to present: - Aquaculture Marketing Director & Extension Specialist, Purdue University and
Illinois-Indiana Sea Grant College Program, West Lafayette, IN
2001 to 2005: - Assistant Professor – Aquaculture Marketing, University of Arkansas at Pine
Bluff, Pine Bluff, AR
REFEREED PUBLICATIONS
BOOK
Engle, C.R. and Quagrainie, K.K. (2006). The Aquaculture Marketing Handbook. Blackwell
Publishing, Ames, Iowa.
REFEREED JOURNAL ARTICLES
1. Ndanga, L.Z.B., K.K. Quagrainie and J.H. Dennis. “Economically Feasible Options for
Increased Women Participation in Kenyan Aquaculture Value Chain.” Aquaculture, accepted
2. He, C., K.K. Quagrainie, and H.H. Wang. “Determinants of Shrimp Importation into the USA:
An Application of an Augmented Gravity Model.” Journal of Chinese Economics and
Business Studies, accepted.
3. Quagrainie, K.K., K.G. Hughes and A. Xing,. “ Delineating Shoppers of Live Seafood in the
Midwestern United States.” Aquaculture Economics & Management, 15 (3) 2011: 155-165
4. Quagrainie, K.K., A. Xing, and K.G. Hughes. “Factors Influencing the Purchase of Live
Seafood in the North Central Region of the United States.” Marine Resource Economics, 26
(1) 2011: 59-74
5. Quagrainie, K.K., C.C. Ngugi, and S. Amisah. “Analysis of the use of credit facilities by smallscale
fish farmers in Kenya.” Aquaculture International, 18 (3) 2010: 393-402
6. Quagrainie, K.K., S. Amisah, and C.C. Ngugi. “Aquaculture Information Sources for Small-
Scale Fish Farmers: The Case of Ghana.” Aquaculture Research, Vol. 40, 2009: 1516-1522.
7. Amisah, S., D. Adjei-Boateng, K. A. Obirikorang and K.K. Quagrainie. “Effects of clam size on
heavy metal accumulation in whole soft tissues of Galatea paradoxa (born, 1778) from the
Volta estuary, Ghana.” International Journal of Fisheries and Aquaculture, 1(2) 2009: 014-
021.
8. Amisah, S. A.B. Gyampoh, P. Sarfo-Mensah, and K.K. Quagrainie. “Livelihood trends in
Response to Climate Change in Forest Fringe Communities of the Offin Basin in Ghana.” J.
Appl. Sci. Environ. Manage. 13(2) 2009: 5 – 15.
9. Adjei-Boateng, D., S. Amisah, and K.K. Quagrainie. “Bacteriological contamination of the
freshwater clam (Galatea paradoxa/born 1778) from the Volta estuary, Ghana.” African
Journal of Microbiology Research, 3(7) 2009: 396-399.
160

10. Kumar, G., Quagrainie, K.K., and Engle, C. “Factors that Influence Frequency of Purchase of
Catfish by U.S. Households in Selected Cities.” Aquaculture Economics and Management,
12(4) 2008: 252-267.
11. Quagrainie, K.K., Hart, S.D., Brown, P.B. “An Exploratory Study of Farmers' View on
Aquaculture Development in Indiana” Journal of Extension, 46(2) 2008. Online Article #
2RIB4.
12. Quagrainie, K.K., Hart, S.D., Brown, P.B. “Consumer Acceptance of Locally Grown Food: The
Case of Indiana Aquaculture Products” Aquaculture Economics and Management, 12(1)
2008:54–70
13. Kaliba, A. R., C.C. Ngugi, J. Mackambo and K.K. Quagrainie. “Economic Profitability of Nile
Tilapia Production in Kenya.” Aquaculture Research, 38(11) 2007: 1129-1136.
14. Kaliba, A. R., S. Amisah, L. Kumah and K.K. Quagrainie. “Economic Analysis of Nile Tilapia
Production in Ghana.” Quarterly Journal of International Agriculture, 46(2) 2007: 105-117.
15. Quagrainie, K.K., T.H. Kuethe, and Engle, C.R. “Arkansas Catfish Farmers and Marketing
Contracts: Some Insights Into Motivations” Aquaculture Economics and Management, 11(1)
2007: 39-51.
16. Neira, I. and Quagrainie, K.K. “Analysis of Risk Behavior in the U.S. Farm-raised Catfish
Market” Marine Resource Economics, 21(4) 2007: 433-443
17. Kaliba, A.R., Osewe, K.O., Senkondo, E.M., Mnembuka, B.V., and Quagrainie, K.K. Economic
Analysis of Nile Tilapia (Oreochromis niliticus) Production in Tanzania. Journal of the World
Aquaculture Society, 37(4) 2006:464-473.
18. Quagrainie, K.K. “IQF Catfish Retail Pack: A Study of Consumers' Willingness to Pay.”
International Food and Agribusiness Management Review, 9(2) 2006: 75-97.
19. Quagrainie, K.K. “Analysis of U.S. Catfish Fillet Market Share Using a Flexible Logistic
Model.” Marine Resource Economics, 21(1) 2006: 33-45.
20. Quagrainie, K.K. and Engle, C.R. “A Latent Class Model for Analyzing Preferences for
Catfish” Aquaculture Economics and Management, 10(1) 2006: 1-14.
21. Wiese, N. Engle, C.R., Green, B. and Quagrainie, K.K. “Reducing Catfish farm Losses Due to
Dockages Assessed by Processing Plants.” Journal of the World Aquaculture Society, 37(1)
2006:60-73.
22. Quagrainie, K.K. and Wiese, N. “Evaluation of Alternative Market Channels for Farm-Raised
Catfish” Journal of Marketing Channels, 13(3) 2006: 51-61
23. McCluskey, J.J. and Quagrainie, K.K. “Measurement of Industry Conduct with a Latent
Structure” Journal of Applied Econometrics 19(7) November/December 2004: 887-897.
24. Quagrainie, K.K. “A Dynamic Almost Ideal Demand Model for US Catfish.” Aquaculture
Economics and Management, 7(5/6) 2003: 263-271
25. Quagrainie, K.K., Unterschultz, J.R., Veeman, M.M., and Jeffrey, S. R. “Testing for Processor
Market Power in the Markets for Cattle and Hogs in Canada” Canadian Journal of
Agricultural Economics, Vol. 51 (3) November 2003: 397-411.
26. Neira, I., Engle, C.R. and Quagrainie, K.K. “Potential Restaurant Markets for Farm-Raised
Tilapia in Nicaragua” Aquaculture Economics and Management, 7(3/4) 2003: 231-247
161

Rebecca Thoms Lochmann
Education
Louisiana State University Zoology BS 1981
Florida Institute of Technol. Marine Biology MS
1988
Texas A&M University Wildlife & Fish. Sci. PhD 1992
Employment
2004-present: Professor, Department of Aquaculture & Fisheries, University of
Arkansas
at Pine Bluff, Pine Bluff, AR
1996-2004: Associate Professor, Department of Aquaculture & Fisheries, University of
Arkansas at Pine Bluff, Pine Bluff, AR
1993-1996: Assistant Professor, Department of Aquaculture & Fisheries, University of
Arkansas at Pine Bluff, Pine Bluff, AR
Scientific & Professional Organizations
World Aquaculture Society
Catfish Farmers of Arkansas
Arkansas Bait and Ornamental Fish Grower’s Association
Sigma Xi
Phi Kappa Phi
Research Overview: Applied and theoretical aspects of aquatic animal nutrition;
emphasis on channel catfish, small cyprinids, and largemouth bass.
Current/Recent Research: Alternative protein and lipid sources in diets of catfish,
largemouth bass, and hybrid striped bass; use of native leaf meals in diets of Nile
tilapia, manipulating diets to improve seafood product quality for human consumers,
prebiotics in small cyprinids and channel catfish, and developing husbandry and
broodstock nutrition protocols to support inland production of Atlantic croaker fry.
Publications (last 5 years)
Faukner, J., S.D. Rawles, A. Proctor, T.D. sink, R. Chen, H. Phillips, and R.T. Lochmann.
2013. The effects of diets containing standard soybean oil, soybean oil enhanced
with conjugated linoleic acids, menhaden fish oil, or an algal docosahexaenoic
acid extract on channel catfish performance, body composition, sensory
evaluation and storage characteristics. North American Journal of Aquaculture
75:252-265.
Lochmann, R. S. Islam, H. Phillips, Z. Adam, and J. Everette. 2013. Effects of dietary
sweet-potato-leaf meal on the growth, non-specific immune responses, total
phenols, and antioxidant capacity in channel catfish Ictalurus punctatus. Accepted
Aug. 10, 2012. Journal of the Science of Food and Agriculture 93(6):1365-1369.
Lochmann, R., C. Engle, G. Kumar, M.H. Li, J.L. Avery, B.G. Bosworth, and C.S. Tucker.
2012. Multi-batch catfish production and economic analysis using alternative
162

(low-cost) diets with corn gluten feed and traditional diets with meat and bone
meal. Aquaculture 366-367:34-39.
Pugliese, N., D. Heikes, C. Engle, B. Bosworth, and R. Lochmann. 2012. Evaluation of
the economics of multi-batch channel catfish Ictalurus punctatus production fed
an industry standard and three alternative diets. Journal of Applied Aquaculture
24:271–282.
Lochmann, R., and H. Phillips. 2012. Effects of diets with 28- or 32% protein and
meatand bone meal or corn gluten feed on performance of golden shiner in
pools. North American Journal of Aquaculture. 74(4):457-462.
Lochmann, R.T., T.D. Sink, and H. Phillips. 2011. Effects of dietary lipid concentration
and a dairy/yeast prebiotic on growth, body composition, and survival of
stressed goldfish challenged with Flavobacterium columnare. North American
Journal of Aquaculture 73:239-247.
Sink, T.D., R.J. Strange, and R.T. Lochmann. 2010. Hatchery methods and natural,
hormone-implant-induced, and synchronized spawning of captive Atlantic
croaker (Micropogonias undulatus). Aquaculture 307: 35-43.
Lochmann, R., T.D. Sink, H. Phillips, and R. Chen. 2010. Evaluation of a dietary
dairy/yeast prebiotic in juvenile golden shiner Notemigonus crysoleucas in ponds.
North American Journal of Aquaculture 72:164-171.
SINK, T.D., R.T. LOCHMANN, C. POHLENZ, A. BUENTELLO, AND DELBERT GATLIN III. 2010.
EFFECTS OF DIETARY PROTEIN SOURCE AND PROTEIN-LIPID SOURCE INTERACTION ON
CHANNEL CATFISH (ICTALURUS PUNCTATUS) EGG BIOCHEMICAL COMPOSITION, EGG
PRODUCTION AND QUALITY, AND FRY HATCHING PERCENTAGE AND PERFORMANCE.
AQUACULTURE 298:251-259.
Sink, T.D., R. Lochmann, and N. Kinsey. 2010. Growth and survival of channel catfish
Ictalurus punctatus fry fed diets with 36 or 45% total protein and all-plant or
animal-protein sources. Journal of the World Aquaculture Society 40:124-129 .
Trushenski, J., and R. Lochmann. 2009. Potential, implications and solutions regarding the
use of rendered animal fats in aquafeeds. American Journal of Animal and
Veterinary Sciences 4(4):108-128.
Li, P., B. Ray, D.M. Gatlin, III, T. Sink, R. Chen, and R. Lochmann. 2009. Effect of handling
and transport on cortisol response and nutrient mobilization of golden shiner
Notemigonus crysoleucas. Journal of the World Aquaculture Society 40: 803-809.
Lochmann, R. R. Chen, F. Chu-Koo, W. Camargo, C. Kohler, and K. Casper. 2009.
Effects of carbohydrate-rich alternative feedstuffs on growth, survival, body
composition, hematology, and non-specific immune response of black pacu,
Colossoma macropomum, and red pacu, Piaractus brachypomus. Journal of the World
Aquaculture Society 40:33-44.
163

MARIA C. HAWS, Ph.D.
Director, Pearl Research and Training Program
Assistant Professor of Aquaculture
Pacific Aquaculture and Coastal Resources Center
University of Hawaii-Hilo / Hilo, Hawaii 96720
Ph. (808) 933-9460 Fax (808) 974-7674 Email: haws@aol.com
E D U C A T I O N
Ph.D. in Wildlife and Fisheries Management, Texas A&M University.
Dissertation topic: Mortality and biochemical correlates during metamorphosis of
the Pacific oyster (Crassostreagigas) and Eastern Oyster (Crassostrea virginica). 1988-
1993.
Bachelor of Science in Biology, Reed College, Portland, OR. 1980-1985.
E M P L O Y M E N T A N D E X P E R I E N C E
Assistant and Associate Professor of Aquaculture/Director of Pearl Research Training
Program/Sea Grant Extension Specialist. January 1, 2009 to present. Pacific Aquaculture
and Coastal Resources Center/University of Hawaii-Hilo and University of Hawaii Sea
Grant Program. Duties: teach aquaculture courses, operate PACRC bivalve hatchery,
conduct extension in Hawaii, U.S. Affiliated Islands and manage international programs.
Conducts research and outreach on climate change adaptation.
Director of Pearl Research Training Program/Sea Grant Extension Specialist/Associate Professor
of Aquaculture. August 1999 to December 2008. Pacific Aquaculture and Coastal
Resources Center/University of Hawaii-Hilo and University of Hawaii Sea Grant Program.
Duties: program development and administration of international pearl research and
training program at University of Hawai’i Hilo. Lead PACRC Coastal Management efforts
in the Pacific Islands. Program emphasizes technical assistance for mariculture and ICZM,
capacity building, institutional strengthening and training/educational efforts.
Coastal Ecologist and Mariculture Policy Specialist. September 1996 to 2009. Coastal Resources
Center, University of Rhode Island.
Director of Operations. January 1996 to October 1999. BioPacific Development, Inc. Oakland,
CA
Regional Aquaculture Extension Agent. May 1996 to August 1996. Center for Tropical and
Subtropical Aquaculture.
Chief of Party and Senior Scientist. July 1994 to August 1995.Cook Island Black-lip Pearl
Culture Project (USAID/RDA International, Inc.).
Pearl Oyster Hatchery Specialist and Senior Scientist. November 1993 to July 1994. Cook
Island Black-lip Pearl Culture Project (USAID/RDA International, Inc.).
SELECTED RECENT PUBLICATIONS AND EXTENSION MATERIALS
Martinez-Cordero, J.F., Q.S.W. Fong and M.C. Haws. 2010. Oyster preferences in Sinaloa,
Mexico. Would buyers pay more for quality, availability? Global Aquaculture Advocate.
November/December, 2010.
Haws, M.C., B. Crawford, S.C. Ellis, N. Jiddawi, A. Mmochi, E. Gaxiola-Camacho, G.
Rodriguez-Dominguez, G. Rodriguez, J. Francis, C. Rivas-LeClair, A. Saborio-Coze, N.
Hernandez, E. Sandoval, K. Dabrowski, M.C. Portella and M. Jaroszewska. 2010
(accepted). Aquaculture research and development as an entry-point and contributor to
natural resources and coastal management. Coastal Management Journal.
Crawford, B.C., M.D. Herrera, N. Hernandez, C. Rivas-LeClair, N. Jiddawi, M. Semba, M.C.
164

Haws. 2010 (accepted). Small Scale Fisheries Management: Lessons from Cockle
Harvesters in Nicaragua and Tanzania. Coastal Management Journal.
Fong, S. W., F.C. Cordero-Martinez and M.C. Haws. 2009. Marketing Extension and Outreach
in Sinaloa, Mexico: A Preliminary Analysis of Preferences for Oysters. Marine Resource
Economics: 24:89-95.
Haws, M.C., E. Ochoa-Moreno and A.A. Rojas Umana. 2008. (eds.). Human Health and
Aquaculture: three case studies of the relationships between human health, the environment
and aquaculture in coastal communities of Sinaloa, Mexico. Pond Dynamics/Aquaculture
Collaborative Research Support Program, Oregon State University. 157 pp.
Haws, M.C. and R. Howerton. 2009. A Shellfish in every pot…everywhere. Ka Pili Ka`i.
University of Hawaii Sea Grant College Program. Vol. 30, No. 3. Fall 2008.
Haws, M.C. and S.C. Ellis. 2009. Recent advances in aquaculture in Micronesia. Ka Pili Ka`i.
University of Hawaii Sea Grant College Program. Vol. 30, No. 3. Fall 2008.
Chang, F., G. Li, M. Haws and T. Niu. 2007. Element concentrations in shell of Pinctada
margaritifera from French Polynesia and evaluation for using as a food supplement. Food
Chemistry 104:1171-1176.
Haws, M.C. 2007. (editor). Assessment of Natural Resources Management Needs for Coastal
and Littoral Marine Ecosystems of the U.S.- Affiliated Pacific Islands: American Samoa,
Guam, Commonwealth of the Northern Marianas, Republic of the Marshall Islands,
Federated States of Micronesia and the Republic of Palau. Technical Report for U.S.
Geological Survey Pacific Island Ecosystems Research Center (PIERC).
Haws, M.C., S.C. Ellis and E.P. Ellis. 2007. Producing half-pearls (Mabe). Western Indian
Ocean Marine Science Association. Zanzibar, Tanzania. 15 pp.
Haws, M.C. and J. Supan. 2007. Edible bivalve culture in Hawai'i, bridging the past, present and
future: a white paper. Pacific Aquaculture and Coastal Resources Center, University of
Hawai'i Hilo.
Haws, M.C., D. Svoboda, S.C. Ellis and J. Kwock. 2007. Marketing and sales skills for
producers of natural products in Hawai`i and the Western Pacific: A training tool box.
Pacific Aquaculture and Coastal Resources Center, University of Hawai'i Hilo. 430 pp.
Other publications:
Principal Author and team member with International Resources Group (IRG) to research and
draft the “State of the Climate Change Vulnerability and Adaption in Selected Pacific
Island Nations”. Published by IRG and the United States Agency for International
Development (USAID), Washington, D.C. February 2012.
Team member with International Resources Group (IRG) to research and draft the, “Asia-Pacific
Regional Climate Change Needs Assessment Report: Published by IRG and the United
States Agency for International Development (USAID), Washington, DC. February 2010.
Team Member with International Resources Group (IRG) to research and draft the “Regional
Strategy for Addressing Climate Change Impacts in Coastal and Marine Areas of the
Western Indian Ocean Region”. Commissioned by the Western Indian Ocean Marine
Sciences Association (WIOMSA) and sponsored by the Swedish International
Development Cooperation Agency (SIDA). March 2011.
165

Alloysius Attah
P.O.Box GP 13380, Accra.
Email: alloysius@farmerline.org, Mobile: +233 (0)249 230 794
I am a passionate, motivated and strategic attractor. I naturally draw synergies between people,
resources, and outcomes and am able to weave them together to drive opportunities. This is evident
in my involvement in building Farmerline while completing my undergraduate degree at the Kwame
Nkrumah University of Science and Technology.
Education
2008 – 2012 Kwame Nkrumah University of Science and Technology, Kumasi,
Ghana
BSc Natural Resource Management
Results: Second Class Honours
Research Title: “Aquafeed Finder: A mobile and web application that provides information
on fish feed/ingredient to Fish farmers in Ghana”
2004 – 2008 Awudome Secondary School, Tsito, Ghana
Agriculture Science
Work Experience
December 2011 – Farmerline Ltd
Co-Founder/CEO
Managed seven (7) months of research and development with a budget of
US$5,000.00, prepared and gave presentations and materials, planned meetings,
trips and special events and supervised three team members.
Recruited and worked with six advisory board members remotely.
Raised US $10,000 from Indigo Trust to support a six month pilot of Farmerline
with 2,000 fish farmers in Ghana
Helped designed and developed the Farmerline platform
Managed the company’s web presence (regular website, twitter and Facebook
updates)
Designing a six month pilot with appropriate impact management plan
Collaborated with Voto Mobile Ltd to develop a voice messaging platform
(go.votomobile.org)
August 2012 -- iCottage Networks, Kumasi, Ghana (icottagenetworks.com)
Front End Developer
I coordinate with the designers and developers to build
Bulk Messaging Platform(txtunlimited.org)
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Banking Applications
Church Management Systems
December 2010 – Alloyworld Ltd, Ghana
Founder/Board Chairman
Founded Alloyworld Ltd (a multimedia company dedicated to producing
photographs and videos on the university campus) whilst in the university. The
company is now managed by a group of University Students from the Kwame
Nkrumah University of Science and Technology.
Volunteering
2011 – to date mFriday.org, Kumasi, Ghana(mFriday is a community of enthusiastic
people who love to learn and develop mobile and web applications.)
Mentor/Member
Organizing pitching sessions for over 200 members of mFriday
Lead several mobile application development sessions for members of mFriday
Contributed to ad campaigns for the mFriday at conferences, on blogs, Facebook
and twitter
Review reports, articles and news on ICTs for Development
Actively involved in networking for the mFriday at conferences and events
Achievements/
Awards
TEDxAccra Speaker
World Summit Youth Award Winner
Global Innovation through Science and Technology Winner
Apps4africa Climate Change Competition Winner
Accra Startup Weekend Winner
MIT-AITI Business Pitching Competition Mentor
Named as one of the Top 50 Africans out of a billion in 2011
Vice Chancellors’ Most Innovative and entrepreneurial Student, KNUST
2011/2012
167

Prof. Stephen AMISAH, PhD
Dean, Faculty of Renewable Natural Resources
Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Telephone: 00 233 3220 60381 (Office) / 00 233 27 740 2633 (Cellular)
E-mail address: steveamisah1@yahoo.co.uk
UNIVERSITIES ATTENDED (with dates)
1977-1981 University of Cape Coast - BSc (Hons) Zoology; Dip. Ed
1984-1986 University of Science & Technology, Kumasi - MPhil (Applied Microbiology)
1988—1989 University of Buckingham, UK - MSc (Fisheries Biology)
1994-1998 University of Hull, Hull, UK - PhD (Fisheries Studies)
EMPLOYMENT HISTORY
Current Position: Associate Professor and Dean of Faculty
Feb. 1990 Appointed Lecturer in Fisheries and Watershed Management, UST
Oct. 2001 Present Promoted to the rank of Senior Lecturer, KNUST
Aug. 2004-July 2006 Dean, Faculty of Renewable Natural Resources, KNUST
2007- 2008 Head, Dept. of Fisheries and Watershed Management, KNUST
Oct.2009 Promoted to the Rank of Associate Professor
2012-Present Elected and Appointed Dean, Faculty of Renewable Natural Resources
RESEARCH INTERESTS
I am interested in investigating the effects of anthropogenic perturbations on aquatic ecosystems and heavy metal
pollution in aquatic ecosystems. I am also interested in working on degraded watersheds and impact of climate
change on watersheds and rural livelihoods. My current researches have focussed primarily on effluent discharges
from aquaculture production units and effects on receiving waters and on economic profitability of fish farming in sub-
Saharan Africa. I have also researched on feeding and ecology of fishes in aquaculture.
Details of Research projects Conducted
i. Economic analysis of Fish production in ponds in Ghana (2003-2005). This research explored the
opportunities and constraints for aquaculture production in Ghana and examined Benefit/Cost analysis and
profitability of some of the most frequently cultured species. (Funded by AquaFish CRSP-USAID)
ii. Use of Computable General Equilibrium (CGE) Models to estimate effects of aquaculture on poverty
reduction in Sub-Saharan Africa (2003-2006). This research was based on collaborative data and
contributions from Ghana, Kenya and Tanzania. Funded by AquaFish CRSP and USAID in collaboration
with Virginia Tech & State University and Purdue University, USA)
iii. Food and feeding ecology of some potential aquaculture fish species of the meteoritic Crater Lake (Lake
Bosumtwi) in Ghana (2006-2007). This sought to explore the ecology and feeding habits of potential
aquaculture species whose exploitation pattern in the Lake Bosumtwi cannot be sustained at current rates
iv. Growth studies of the African catfish, Clarias gariepinus, under varying feed and culture conditions
(2006-2007). This was a combination of several studies, which included examining alternative plant protein
sources and various periphyton substrates for growth of Clarias gariepinus.
v. The use of Choice modelling techniques and probit analysis to determine Aquaculture information flow
in Ghana and credit use in Kenya (2006-2008). This study examined how aquaculture information is
disseminated to the fish farmer and how credit, when available to the fish farmer, is used in Ghana and Kenya.
(Funded by AquaFish CRSP-USAID)
vi. Impact of Climate change on watersheds and implications for their dependent communities (2004-2008).
The effect of climate change over several decades was examined and time series of data were analysed to
elucidate trends and also impacts on catchment communities.
vii. Ecological studies of the Volta estuary clam, Galatea paradoxa, in Ghana (2006-2009).
Whole soft tissues of Galatea paradoxa remain a delicacy in the Volta estuary and lower catchment areas. The
study examined the ecology of the clam and anthropogenic impacts on the organism. The organism has the
tendency to accumulate heavy metals in its tissues, which may be hazardous to consumers. The organism was
168

examined for heavy metal content in whole soft tissues. (Funded by the International Foundation for Science
(IFS). Bacteriological examinations were also conducted on clams at Ada and Aveglo in the Volta estuary to
assess the extent of contamination and safety for human consumption. (Funded by IFS)
SCIENTIFIC PUBLICATIONS
1. S. Amisah, K. A. Obirikorang and D. Adjei-Boateng (2011). Bioaccumulation of Heavy metals in the Volta
Clam, Galatea paradoxa (Born, 1778) in relation to their geoaccumulation in benthic sediments of the Volta
Estuary, Ghana. Water Quality, Exposure and Health 2: 147-156.
2. D. Adjei-Boateng, K. A. Obirikorang, S. Amisah, H. A. Madkour • F. A. Otchere (2011). Relationship Between
Gonad Maturation and Heavy MetalAccumulation in the Clam, Galatea paradoxa (Born 1778)from the Volta
Estuary, Ghana
3. Obirikorang, K.A., Adjei-Boateng, D Madkour, H.A., Otchere, F.A. and Amisah, S. Estuary (2013). Length- weight
relationship of the freshwater clam, Galatea paradoxa from the Volta).Journal of Biological Sciences 16(4) 185-
189)
4. Obirikorang, K.A., Madkour, H.A., Amisah, S., Adjei-Boateng, D. and Otchere, F.A. (2011). Heavy Metal
Accumulation (Mn, Zn, Fe and Hg) in the Surface Sediments of the Volta Estuary, Ghana. Australian Journal of
Basic and Applied Sciences, 5(9): 564-570.
5. Adjei-Boateng, D. Obirikorang, K. A. and Amisah, S. (2010). Bioaccumulation of heavy metals in the tissues of
the Clam Galatea paradoxa and sediments from the Volta estuary, Ghana. Int. J. Environ. Res 4(3): 533-540.
6. K. A. Obirikorang, S. Amisah, D. Adjei-Boateng, H. A. Madkour, F. A. Otchere (2010) Mercury Accumulation in
the Clam, Galatea paradoxa (Born 1778) at the Volta Estuary, Ghana. Bull Environ Contam Toxicol 85:497–501.
7. K. K. Quagraine, Charles C. Ngugi, Stephen Amisah (2010). Analysis of the use of credit facilities by small
scale fish farmers in Kenya. Aquaculture International. 18:393-402.
8. D. Adjei-Boateng, B. Owusu Appiah and S. Amisah (2010). Effect of sandy and muddy substrates on the growth
and survival of the freshwater clam Galatea paradoxa (Born, 1778). Aquaculture Research 1-5. doi:
10.1111/j.1365-2109.2009.02464.x
9. S. Amisah, D. Adjei-Boateng, K. A. Obirikorang and K. K. Quagrainie (2009). Effects of clam size on heavy
metal accumulation in whole soft tissues of Galatea paradoxa (Born, 1778) from the Volta estuary, Ghana.
International Journal of Fisheries and Aquaculture 12, 014-021.
10. Amisah, S, Gyampoh, B. A, Sarfo-Mensah, P. and Quagrainie, K. K. (2009). Livelihood trends in response to
Climate Change in Forest fringe communities of the Offin basin in Ghana. J. Appl. Sci. Envrion. Manage 13 (2),
5-15.
11. Amisah, S. Oteng, M. A; Ofori, J. K. (2009).Growth performance of the African catfish, Clarias gariepinus, fed
varying inclusion levels of Leucaena leucocephala leaf meal (2009). J. Appl. Sci. Envrion. Manage 13 (1), 21-26.
12. K. A. Obirikorang, D. Adjei-Boateng, S. Amisah (2009). Consumption of the clam, Galatea paradoxa (Born
1778) in Ghana: Human Health implications with reference to heavy metals. Water Quality, Exposure and Health
1: 191-201
13. Adjei-Boateng, Daniel, Amisah Stephen, and Quagrainie (2009). Bacteriological contamination of the
freshwater clam Galatea paradoxa (Born, 1778) from the Volta estuary, Ghana. African Journal of Microbiology
Research 3 (7), 396-399.
14. Kwamena K. Quagrainie, Stephen Amisah & Charles Ngugi (2009). Aquaculture information sources for small
scale fish farmers: the case of Ghana. Aquaculture Research, 40, 1516-1522.
15. B. A. Gyampoh, S. Amisah. M. Idinoba and J. Nkem (2009). Using traditional knowledge to cope with climate
change in rural Ghana. Unasylva 231/232, Vol. 60, 70-74
16. Amisah, S. and Agbo, N. W. (2008). An investigation into the food and feeding ecology of a potential
aquaculture candidate, Sarotherodon galilaeus multifasciatus, in a meteoritic crater lake in Ghana. J. Appl. Sci.
Envrion. Manage 12 (3), 15-18.
169

Regina E. Edziyie
Lecturer, Dept of Fisheries and watershed Mgmt,
Faculty of Renewable Natural Resource (FRNR)
Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Telephone: +233 572921090
E-mail address: edziyie@yahoo.co.uk
EDUCATION
Ph.D. 2011 Environmental Sciences, University of North Texas (UNT), Denton, TX
M.S. 2009 Aquaculture/Fisheries, Univ. of Arkansas (UAPB), Pine Bluff, AR
M.S. 2004 Applied Geography (Water Resources), UNT, Denton, TX
BSc. 2000 Natural Resources Mgt. Kwame Nkrumah Univ. of Sc. & Tech. (KNUST), Ghana
PROFESSIONAL EMPLOYMENT
Present: Lecturer, Dept. of Fisheries and Watershed Management, FRNR, KNUST, Ghana
2011-2012 Post Doctoral Fellow at UNT (Science Education Research): writing grants and
Research in Environmental Education and implementation of programs and projects
2011 – 2012 Adjunct Faculty, Earth Science, Columbia Southern University, Alabama
(Online).
2011 Adjunct Faculty, Environmental Science, Kaplan College Dallas, Texas (Adult classes).
2011 Instructor, Environmental Science lecture, University of North Texas, Denton, Texas.
2004 – 2010 Instructor, Environmental Science, & Contemporary Biology laboratory, UNT,
TX.
RESEARCH INTERESTS
Increasing aquaculture productivity in Ghana
Water quality and plankton communities in pond aquaculture and other culture systems
Aquatic ecology and toxicology in natural systems: Water, biota, (algae, clams and fish) and
sediments
Science education and student mentoring
PROFESSIONAL SERVICE
Co-moderator, resource person, attendee, Women in Aquaculture, AquaFish Collaborative
Research Support Program, Workshop Programme 8-9th January 2013, International
Centre for Innovative Learning Kwame Nkrumah University of Science and Technology,
Kumasi & Akosombo, Ghana
Co-organizer and attendee, Research Experiences for Teachers(RET) Workshop, National
Science Foundation RET site University of North Texas(UNT), 5th November, 2011.
Discovery Park, UNT, Denton, Texas.
Participant, Building Stronger Universities, GEP Strategizing Outreach for Sustainable
Agriculture and Aquatic Products in Ghana, 25th March, 2013, International Centre for
Innovative Learning, Kumasi, Ghana
Guest Speaker:
Arkansas State University, 05- 07, 2004. Effect of propanil on water quality & plankton
in aquaculture ponds.
University of North Texas, Denton; Sediment Toxicology Class, October 29, 2009. Fate,
transport and effects of triclosan and triclocarban in aquatic ecosystems.
University of Arkansas at Pine Bluff. September 06, 2011. Antimicrobials in waste-water
receiving streams.
170

Member, Organizing Committee Regional SETAC meeting, 2005.
Member, Student Activities Committee US Aquaculture Society, 2006.
Member, Search Committee for GIS Faculty Department of Geography, UNT.
AWARDS
2009- Best TA award, Environmental Science
2007- 2009 – Beth Baird Scholar, Biology Department, UNT
2007-2nd Place Poster Award (co-author) National SETAC Meeting.
2006/2007 - USO Award, UNT
2005 - Best Poster Award (co-author) Regional SETAC Meeting.
2005/2006 - Multicultural Scholastic Award.
2004 - Best Abstract, US Aquaculture Society.
2000 - C.P. Morrice Best Student & Project Awards, Fisheries Dept. KNUST
PUBLICATIONS
1. Perschabcher, P. and R. Edziyie, G. M. Ludwig. 2 012. Row Crop Herbicide Drift Effects
on water bodies and aquaculture. In Herbicides/ Book 3. Ed. M.N. A. E. Hassanem. Intech
Publishers.
2. Gopinath, N.C., Peter, P., M. Venables, B. J. Edziyie, R. E., Constantine, L. A., and D. B.
Huggett. 2011. Tissue-specific uptake and bioconcentration of the oral contraceptive
norethindrone in two freshwater fishes. Arch Environ Contam Toxicol .DOI 10.1007/s00244-
011-9691-x (published online on June 28, 2011).
3. Ludwig, G.M., Perschbacher, P. W., and Edziyie, R. 2010. The effect of the dye Aquashade
on water quality, phytoplankton, zooplankton, and sunshine bass fingerling production in
fertilized culture ponds. Journal of World Aquaculture Society,41(S1):40-48
4. Perschbacher, P. W., G.M. Ludwig and Edziyie, R. 2008. Effects of atrazine drift on
production pond plankton and water quality using experimental mesocosms. Journal of
World Aquaculture Society, 39(1):126-130
5. Coogan, M. A., Edziyie, R.E., La Point, T. W. and Venables, B.J.2007. Algal
bioaccumulation of triclocarban, triclosan and methyl triclosan in a North Texas Wastewater
Treatment Plant. Chemosphere, 67(10):1911-1918.
6. Neal, J.W., S. Shreay, N.E. Heitman, M.C. Leao, K. R. Winningham, R.E. Edziyie and C.
Jackson. 2004. Hybrid striped bass in urban ponds: a pilot study on angler demographics,
attitudes and fishing success. Proc. Annual Conf. Southeast. Assoc., Fish & Wildlife Agencies
58: 130-138.
MEETINGS
Over 20 oral/poster presentations at American and World Aquaculture conferences, Association
of American Geographers Association, Sigma Xi meeting, Regional and National Society of
Environmental Toxicology and Chemistry meetings, American Fisheries meeting etc.
171

Dr. Nelson Winston Agbo
Dept. of Fisheries & Watershed Mgt., FRNR, KNUST, Kumasi, Ghana
Telephone:+233 248220311;
E-mail: nelagbo@yahoo.co.uk; nelagbo@gmail.com
Nationality: Ghanaian
Tertiary Education
Stirling University, Scotland, UK, 2004-2008. PhD Aquaculture
Kaliningrad State Technical University, Kaliningrad, Russia, 1988-1994. MSc.
Water Bioresources & Aquaculture.
Employment History
Head, Department of Fisheries & Watershed Management, Faculty of Renewable
Natural Resources, Kwame Nkrumah University of Science & Technology,
Kumasi. 2012-Present.
Demonstrator; Institute of Aquaculture, University of Stirling. Tutorials and
demonstration to undergraduate students, 2004-2006.
Lecturer; College of Renewable Natural Resources, Sunyani. Part-time: Lecturing
Aquaculture & Fisheries Management, 2000- 2004.
Lecturer; Faculty of Renewable Natural Resources, Kwame Nkrumah University
of Science & Technology, Kumasi. 1996-Present.
Other Experiences
HC Co-PI; Aquafish Collaborative Research Support Program (CRSP),
‘Experimental pond unit assessment of AquaFish CRSP research sites in Ghana’
2012.
Research Officer, Wastewater-fed aquaculture production for sustainable
sanitation in Ghana, collaboration with International Water Management
Institute (IWMI), 2011-2012.
HC PI; Aquafish Collaborative Research Support Program (CRSP), ‘Development
and diversification of species for aquaculture’ 2009-present.
Coordinator Ghana, Sustainable Aquaculture Research Networks in Sub Saharan
Africa (SARNISSA).
Research Officer; Fish seed production and feed formulation and development,
Agriculture Sub-sector Improvement Programme (AgSSIP), Ghana, 2002-2004.
Resource scientist; for Friends of the Earth (FOE), Lake Bosomtwi Fishery: The
threat to biodiversity and livelihoods in the Bosomtwi Basin, 2003
Recent Publications
Adjei-Boateng D., Essel K.M. and Agbo N.W. (2012) Growth and survival of the
Freshwater Clam, Galatea paradoxa (Born 1778) cultured on different substrata at
the Volta estuary, Ghana. Aquaculture Research, 43: 1480-1486
Tenkorang A., Yeboah-Agyepong M., Buamah R., Agbo N., Chauhdry R. and
172

Murray A. (2012) Promoting sustainable sanitation through wastewater-fed
aquaculture: a case study from Ghana. Water International, DOI:
10.1080/02508060.2012.733669
Agbo N. W, Adjei-Boateng D. and Jauncey K. (2011). The potential of groundnut
(Arachis hypogaea L.) by-products as alternative protein sources in the diet of Nile
tilapia (Oreochromis niloticus). Journal of Applied Aquaculture, 23:4, 367-378.
Agbo N.W., Amisah S, Frimpong E. and Quagrainie K. 2011. Development and
Diversification of indigenous species for Aquaculture in Ghana. Aquanews 26 (3):
summer-Fall.
Agbo N.W., Madalla N. and Jauncey K. (2011) Effects of dietary cottonseed meal
protein levels on growth and feed utilization of Nile tilapia, Oreochromis niloticus
L. Journal of Applied Science and Environmental Management. 15 (2): 235-239.
Anane-Taabeah G., Frimpong E.A., Amisah S. and Agbo N.W. (2011) Constraints
and opportunities in cage aquaculture in Ghana. In: Liu Liping and Kevin
Fitzsimmons (Eds.) Proceedings of the Ninth International Symposium on Tilapia in
Aquaculture (ISTA 9). 21-24 April 2011. Shanghai, pp. 158 – 165.
Madalla N., Agbo N.W. and Jauncey K. (2010) Evaluation of aqueous extracted
moringa leaf meal as a protein source for Nile Tilapia juveniles. Tanzania Journal
of Agricultural Sciences. 10 (1): 40-49.
Agbo N. W., Madalla N. and Jauncey K. (2009) The biochemical composition of
some Ghanaian oilseed by-products and their digestibility by Nile tilapia,
Oreochromis niloticus L. Journal of the Ghana Science Association. 11 (2): 136-148.
Agbo N. W. (2009) Development of small-scale commercial fish farm in the
Ashanti Region of Ghana: A case study of Mr. Poku Gyinaye of “Bosomtwi
Integrated Aqualife Village”. In: Aquaculture Compendium. Wallingford, UK:
CAB International.
Amisah S. and Agbo N. W. (2008). An investigation into the food and feeding
ecology of a potential aquaculture candidate, Sarotherodon galilaeus
multifasciatus, in a meteoritic crater lake in Ghana. Journal of Applied Science and
Environmental Management. 12 (3) 15-18
Current Research Interest
Aquaculture systems
Aquaculture nutrition studies on the use of plant products as source of protein
Development and diversification of species for aquaculture
173

Name Date of
birth
Sebastian 15
Wilson
Chenyambuga
th April
1965
Sebastian Wilson Chenyambuga
Place of
birth
Magu,
Tanzania
EDUCATION QUALIFICATIONS
Nationality Marital Religion Language
status
spoken
Tanzanian Married Christian Swahili
and
English
Place Year Certificate obtained
Sokoine University of
Agriculture (SUA), P.O.Box
3000, Morogoro, Tanzania
1997 - 2002 PhD (Animal breeding
and Genetics)
SUA 1992 - 1994 M.Sc .Agriculture
(Animal Production)
SUA 1989 - 1991 B.Sc. Agriculture
SHORT COURSES/WORKSHOPS
(i) Genome Analysis and Molecular Genome Analysis at the Department of Animal Science,
Wageningen Agricultural University, The Netherlands, January – March 1998.
(ii) Methodologies for on-farm surveys and characterisation of Animal Genetic Resources,
ILRI, Addis Ababa, Ethiopia, June 1 – 6, 1998.
(iii) Bioinformatics, Egerton University, Kenya, September 6 – 11, 2004.
(iv) Policy and Risk Assessment and Management of Genetically Modified Crops, Nairobi,
Kenya, January 25 - 29, 2005.
(v) Capacity building for sustainable use of animal genetic resources in developing countries,
ILRI, Addis Ababa, Ethiopia 3 rd – 21 st November 2008
(vi) Establishment of National and Regional Livestock gene banks for ASARECA region, ILRI,
Addis Ababa, Ethiopia, 13 th – 17 th December 2010.
EMPLOYMENT RECORD
Year 1991 - 1995 1995 - 1997 2000 - 2003 2003 - 2006 2006 - 2010 2010
todate
-
Occupation Livestock Research Assistant Lecturer Senior Associate
Research
Officer
Assistant Lecturer
Lecturer Professor
Employer Ministry of Sokoine Sokoine Sokoine Sokoine Sokoine
Agriculture University University University University University
of
of
of
of
of
Agriculture Agriculture Agriculture Agriculture Agriculture
PUBLICATIONS FOR THE LAST FOUR YEARS
Chenyambuga S W, Waiswa C, Saimo M, Ngumi P and Gwakisa P S (2010). Knowledge and
perceptions of traditional livestock keepers on tick-borne diseases and sero-prevalence
of Theileria parva around Lake Victoria Basin. Livestock Research for Rural
Development. Volume 22, Article # 137 . Retrieved August 16, 2010, from
http://www.lrrd.org/lrrd22/7/chen22137.htm
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Chenyambuga S.W., M.M. Kibodya, B.V. Mnembuka, K.O. Osewe, R. Lochmann and K.
Quagrainie (2010). Use of Moringa oleifera and Leucaena leucocephala to improve cost
efficiencies in tilapia (Oreochromis niloticus ) feed. Presented at World AQUACULTURE
2010, Town and Country Convention Centre, San Diego, USA, 2 nd – 5 th March 2010.
Karimuribo E D, Chenyambuga S W, Makene V W and Mathias S (2011). Characteristics and
production constraints of rural-based small-scale pig farming in Iringa region, Tanzania.
Livestock Research for Rural Development. Volume 23, Article #172. Retrieved August 5,
2011, from http://www.lrrd.org/lrrd23/8/Kari23172.htm
Ernest M. Eblate, Kusiluka J. Lughano, Chenyambuga W. Sebastian, Msoffe L. Peter, Røed H.
Knut (2011). Polymorphic microsatellite markers for genetic studies of African antelope
species. African Journal of Biotechnology 10, 11817-11820
Chenyambuga S.W., Buru J., Mnembuka B.V., Madalla, N., Lochmann R. and Quagrainie K. (2011).
Comparative growth performance of Nile tilapia (Oreochromis niloticus)grown under
mixed-sex, monosex and polyculture systems in small-scale ponds in Tanzania. Presented
at the 9 th Asian Fisheries and Aquaculture Forus (9AFAF), Shanghai Ocean University,
Shanghai, China, 21 – 25 th April 2011.
Chenyambuga S W, Komwihangilo D M and Jackson M (2012). Production performance and
desirable traits of Small East African goats in semi-arid areas of Central Tanzania.
Livestock Research for Rural Development. Volume 24, Article #118. Retrieved July 3, 2012,
from http://www.lrrd.org/lrrd24/7/chen24118.htm
Stella Bitanyi, Marit Nesje, Lughano JM Kusiluka, Sebastian W Chenyambuga and Bjørn P
Kaltenborn (2012). Awareness and perceptions of local people about wildlife hunting in
western Serengeti communities. Tropical Conservation Science Vol. 5(2):208-224. Available
online: www.tropicalconservationscience.org
P.L. Mwambene, A.M. Katule and S.W. Chenyambuga and P.A.A. Mwakilembe. (2012). Fipa
cattle in the south-western highlands of Tanzania: socio-economic roles, traditional
management practices and production constraints. Animal Genetic Resources 51: 1 – 14.
Available on CJO 2012 doi:10.1017/s2078633612000112.
http://journals.cambridge.org/abstract_s2078633612000112.
http://journals.cambridge.org/abstract_s2078633612000124.
P.L. Mwambene, A.M. Katule and S.W. Chenyambuga and P.A.A. Mwakilembe. (2012). Fipa
cattle in the south-western highlands of Tanzania: morphometric and physical
characteristics. Animal Genetic Resources 51: 15 – 29. Available on CJO 2012
doi:10.1017/s2078633612000136.
http://journals.cambridge.org/abstract_s2078633612000136.
P.L. Mwambene, A.M. Katule and S.W. Chenyambuga, Y. Plante and P.A.A. Mwakilembe.
(2012). Fipa cattle in the south-western highlands of Tanzania: molecular
characterization. Animal Genetic Resources 51: 31 – 43. Available on CJO 2012
doi:10.1017/s2078633612000392.
http://journals.cambridge.org/abstract_s2078633612000392.
Chenyambuga S.W., Madalla N. and Mnembuka B.V. (2012) Management and value chain of
Nile tilapia cultured in ponds of small-scale farmers in Morogoro region, Tanzania.
Presented at the International Institute of Fisheries Economics and Trade (IIFET)
conference, Kilimanjaro Hotel, Dar es Salaam, Tanzania, 16 – 20 th July 2012.
Omary D. Kitojo, Hegi Shighulu, Nazael Madalla, Berno V. Mnembuka, Sebastian W.
Chenyambuga. (2012). Assessment of feeding strategies based on Moringa oleifera leaf meal
and Sunflower seed cake as sources of protein in diets of Nile tilapia (Oreochromis nilotics).
Presented at the 35 th Annual Scientific Conference of Tanzania Society of Animal
Production , Olasit Garden, Arusha, Tanzania, 23 rd to 26 th October 2012
175

Nazael Amos Madalla
PERSONAL HISTORY
Surname: Madalla
Other given names: Nazael Amos
Date of Birth: 2 nd December 1971
Nationality: Tanzanian
Employer: Department of Animal Science and Production,
Sokoine University of Agriculture
P.O. Box 3004, Chuo Kikuu, Morogoro, Tanzania.
Position: Senior Lecturer
Telephone: +255 769 220 212/ +255 719 489 171
E-mail: nmadalla@suanet.ac.tz OR nmadalla@gmail.com
ACADEMIC QUALIFICATION:
PhD (Aquaculture Nutrition), 2004 – 2008, University of Stirling, UK
M.Sc. (Aquaculture), 1999 – 2001, Ghent University, Belgium.
B.Sc. (Animal Science), 1994 – 1998, Sokoine University of Agriculture, Tanzania.
PUBLICATIONS
Agbo, N.W., Madalla, N. & Jauncey, K. (2011) Effects of dietary cottonseed meal
protein levels on growth and feed utilization of Nile tilapia, Oreochromis niloticus
L. Journal of Applied Sciences and Environmental Management 15 (2) 235 – 239
Madalla, N., Wille, M. & Sorgeloos, P. (2010) Effects of dietary nucleotides on growth rate
and disease resistance of crustaceans using axenic Artemia culture tests. Tanzania
Journal of Agricultural Sciences, Volume 10, Number 2, 32-39.
Madalla N., Agbo, N.W. and Jauncey K. (2010) Evaluation of Aqueous Extracted
Moringa Leaf Meal as A Protein Source for Nile Tilapia Juveniles. Submitted to
Tanzania Journal of Agricultural Sciences, Volume 10, Number 2, 40-49.
Agbo N. W., Madalla N. and Jauncey K. (2009) The biochemical composition of some
Ghanaian oilseed by-products and their digestibility by Nile tilapia, Oreochromis
niloticus L. Journal of the Ghana Science Association (in press)
Mutayoba, S. K., N. Madalla & A. D. Maeda – Machangu. (2005). Effect of Different
Management Practices on Incidences of Coccidiosis and Performance of Broiler
Chicken. Tanzania Veterinary Journal (In Press)
Sarwatt, S. V., Milang'ha, M. S., Lekule, F. P. & Madalla, N. (2004). Moringa oleifera and
cottonseed cake as supplements for smallholder dairy cows fed Napier grass
Livestock Research for Rural Development, Volume 16, Number 6, June 2004
Chenyambuga S.W., Madalla N. and Mnembuka B.V. (2012) Management and value
chain of Nile tilapia cultured in ponds of small-scale farmers in Morogoro region,
Tanzania. Presented at the International Institute of Fisheries Economics and Trade
(IIFET) conference, Kilimanjaro Hotel, Dar es Salaam, Tanzania, 16 – 20th July
2012.
Shigulu, H., Madalla, N., Mnembuka, B.V. & Chenyambuga, S.W. (2011) Effect of
Substituting Sunflower Seed Cake Meal and Soybean Meal with Moringa Leaf Meal
in Oreochromis niloticus diets. Paper presented to 34 th TSAP conference held at
Olasit Garden, Arusha Tanzania from 25 th to 27 th October 2011
Agbo, N. W., Madalla, N. and Jauncey, K. (2009). Biochemical composition of some
oilseed by-products and their digestibility by Nile tilapia (Oreochromis niloticus L.).
26 th Biennial Conference of Ghana Science Association, Cape Coast University,
176

Cape Coast, August 4 – 9
Madalla, N., Wille, M. & Sorgeloos, P. (2005). Beneficial Effects of Nucleotides in
Crustaceans: Axenic Artemia Culture as a Test Case. In Hendry, C.I., Van Stappen,
G., Wille, M., & Sorgeloos, P. (eds). Proceedings of Larvi ’05 – Fish and Shellfish
Larviculture Symposium held on 5 – 8 th September 2005, Ghent University,
Belgium. pp 302 – 305.
B. Mnembuka, N. Madalla, E. Mtinda, T. Msogoya, A. Luzi – Kihupi, F. Rwambali, O.
Kajitanus, & E. Senkondo. (2004). Assessment of potentials and constraints of
integrated aquaculture in selected areas of Tanzania, presented to 31st TSAP
conference held on 5th to 7th October 2004, Kilimanjaro, Tanzania.
S.K. Mutayoba, N. Madalla & A. D. Maeda – Machangu (2000). Effect of Different
Management Practices on Incidences of Coccidiosis and Performance of Broiler
Chicken. Proceedings of 3 rd All Africa Conference of The Egyptian Society of
Animal Production, Alexandria, Egypt, 6th – 9th November 2000 pp 431 – 435.
Mnembuka, B.V., N. Madalla, E. Mtinda, T. Msogoya, A. Luzi – Kihupi, F. Rwambali, O.
Kajitanus, & E. Senkondo. (2004). Baseline report for a project on promotion of
integrated aquaculture with agriculture for improved livelihoods of rural
communities submitted to Future Opportunities in Agricultural Learning (FOCAL)
programme
Katule A.M., B. V. Mnembuka, N. Madalla, H. Lamtane & R. Mnubi (2010). Ufugaji
Mseto wa Samaki. Kimehaririwa na Kurwijila, L.R., E. K. Batamuzi & D. M.
Gimbi. Kimetolewa na Programmu ya PANTIL, SUA. ISBN 978 9987 6409 42
RESEARCH PROJECTS
Development of Tilapia and Catfish Feeds for Sustainable Aquaculture Development in
Tanzania funded by Government of Tanzania through COSTECH from 2011-2013.
Principal Investigator
Improving the Productivity of Nile tilapia through Selective Breeding and Mass Production of
Fingerlings funded by Norwegian Government through EPINAV program 2011-2013.
Research Team Member
Development of Appropriate Hatchery Technology for African Catfish in Tanzania funded by
Government of Tanzania through COSTECH 2011-2013. Research Team Member
Enhancing the Profitability of Small Aquaculture Operations in Ghana, Kenya, and Tanzania
funded by USAID through Aquafish CRSP 2011 – 2013. Research Team Member
Impact of Climate Change on Mangrove Ecosystems and Associated Fishery Resources along
the Tanzanian Coast funded by Norwegian Government through CCIAM Programme
2010 – 2014. Research Team Member
Promotion of Integrated Aquaculture with Agriculture for Improved Livelihoods of Rural
Communities funded by Norwegian Government through FOCAL 2003 – 2011. Research
Team Member
177

GIFTY ANANE-TAABEAH
Curriculum Vitae
Current address
P. O. Box NG 337,
243 864 931
Phones: (+233) 269 628 241; (+233)
Nungua, Accra E-mail: giftya85@vt.edu
Ghana Alternative e-mail: ananetaabeah@gmail.com
Research Interests
Fish Ecology, Freshwater Ecology, Ichthyology, Taxonomy and Systematics of
Freshwater Fishes,
Ecological Modeling, Environmental Assessment, and Ecological and Socio-
Economic Aspects of
Aquaculture
Education
M.Sc. (May, 2012) Fisheries and Wildlife Sciences: Virginia Polytechnic Institute & State
University (Virginia Tech).
MS thesis title: Harnessing the opportunities and overcoming constraints to
widespread adoption of cage aquaculture in Ghana.
B.Sc. (June, 2008) Natural Resources Management: Kwame Nkrumah University of
Science
and Technology (KNUST), Kumasi, Ghana.
BSc thesis title: Effect of different diets on the organoleptic qualities of cooked
Nile Tilapia, Oreochromis niloticus (Linn.).
M.Sc. Curriculum Highlights
Ichthyology, Fish Ecology, Stream Ecology, Social Science Research Methods,
Statistics in Research I & II, Natural Resources and Environmental Economics, Linear
Modeling on Ecological Data, Advanced Human Dimensions of Fisheries and Wildlife,
Fluvial Landscape Ecology
Employments
June 2012 – Present: Research Associate, Dept. of Fisheries & Watershed
Management, KNUST.
Jan. 2010 – May 2012: Graduate Research Assistant Dept. of Fish & Wildlife
Conservation, Virginia
Tech
Sep. 2008 – Dec. 2009: Teaching and Research Assistant Dept. of Fisheries &
Watershed
Management, KNUST
Internships and Trainings
June - July 2006: Student Intern, Water Research Institute, Fisheries Sector, Accra,
Ghana.
178

June - July 2008: Aquaculture Entrepreneurship Trainee, Ministry of Fisheries in
collaboration with
Kumah Farm Complex, Kumasi, Ghana
ACRSP/AquaFish Research Experience
June 2012 – Dec. 2012: Host Country co-PI on the project, “Assessment of the tilapia
value chain in Ghana.”
Jan 2009 – Dec 2009: Field crew member on the project, “Characterization of pond
effluents and biological and physicochemical assessment of receiving waters in Ghana.”
Sep 2008 – Dec 2009: Field crew member on the project, “Fish supply chain and group
marking development in Ghana.”
Awards and Highlights
Philanthropic Education Organization (PEO) International Women Peace
Scholarship (2010/2011 Academic year disbursed through Virginia
Tech University)
Graduate Student Profile: AquaNews (Bulletin of the USAID AquaFish CRSP)
Summer 2010
http://aquafishcrsp.oregonstate.edu/Documents/News%20&%20Events/Aquanew
s%20Summer%20final_102510.pdf
Professional Associations
American Fisheries Society (AFS), Virginia Tech Chapter (2010 -2012)
World Aquaculture Society (WAS, since 2010)
North American Aquaculture Society (since 2010)
Conferences and Workshops Attended
February 2013: Triennial Meeting of the World Aquaculture Society (Aquaculture
2013). Nashville, TN, USA. Presenter.
January 2013: Women’s Training Program for Commercial Aquaculture. KNUST,
Kumasi, and ARDEC, Akosombo, Ghana. Co-organizer and Trainer.
July 2012: Biennial Conference of the International Institute of Fisheries and
Economics & Trade (IIFET 2012) Dar es Salaam, Tanzania. Presenter.
July 2012: Workshop on Value Chain Analysis for Aquatic Products. Zanzibar
Grand Palace Hotel, Zanzibar, Tanzania. Attendee.
February – March 2012: Aquaculture America 2012, Las Vegas, NV, USA.
Presenter.
March 2012: 28 th Annual Graduate Student Assembly (GSA)
Research symposium. Blacksburg, VA, USA. Presenter.
July 2010: Experimental Design and Analysis for Aquaculture Professionals.
KNUST, Kumasi, Ghana. Co-organizer.
November 2009: Aquaculture and Environment Workshop. KNUST, Kumasi,
Ghana. Co-organizer.
179

EMMANUEL A. FRIMPONG
Institution Major Degree &Year (s)
University of Science & Technology, Ghana Natural Resources Management BS-1997
University of Arkansas at Pine Bluff Aquaculture and Fisheries MS-2001
Virginia Polytech. Inst. & State University Statistics MS-2009
Purdue University Resource Policy/Fisheries & Aquatic Sci. PhD-2005
Virginia Polytech. Inst. & State University Fish Ecology & Bioassessment-Postdoc. 8/2005-8/2006
Appointments
Apr. 2013 – Present Associate Professor
Virginia Polytech. Inst. & State University, Dept. of Fish & Wildlife Conservation
Dec. 2007 – Mar. 2013 Assistant Professor
Virginia Polytech. Inst. & State University, Dept. of Fish & Wildlife Conservation
Aug. 2006 – Dec. 2007 Research Scientist
Virginia Polytech. Inst. & State University, Dept. of Fisheries & Wildlife Sciences
Aug. 2005 – Aug. 2006 Postdoctoral Associate
Virginia Polytech. Inst. & State University, Dept. of Fisheries & Wildlife Sciences
Peer-Reviewed Publications in the Last 4 Years:
Peoples, B. K., and E. A. Frimpong. (In press). Evidence of mutual benefits of nest association among
freshwater cyprinids and implications for conservation. Aquatic Conservation.
Ansah, Y. B., E. A. Frimpong, and S. Amisah. (In press). Characterization of potential pond effluents and
physicochemical and microbial assessment of effluent-receiving streams in central Ghana. African
Journal of Aquatic Science.
Stich, D. S., V. Dicenzo, E. A. Frimpong, Y. Jiao, and B. R. Murphy. 2013. Growth and population size of
grass carp incrementally stocked for hydrilla control. North American Journal of Fisheries
Management 33:14-25
Ansah, Y. B., E. A. Frimpong, and S. Amisah. 2012. Biological assessment of aquaculture effects on
effluent-receiving streams in Ghana using structural and functional composition of fish and
macroinvertebrate assemblages. Environmental Management 50:166-180.
Frimpong, E. A., and M. Henebry. 2012. Short-term effects of formalin and ethanol fixation and
preservation techniques on weight and size of fish eggs. Transactions of the American Fisheries
Society. 141:1472-1479
Pendleton, R. M., J. J. Pritt, B. K. Peoples, and E. A. Frimpong. 2012. The strength of Nocomis nest
association contributes to patterns of rarity and commonness among New River, Virginia cyprinids.
American Midland Naturalist 168:202-217
Peoples, B. K., and E. A. Frimpong. 2012. A snapshot analysis of age distributions of fishes in urban and
forested Virginia streams. Urban Ecosystems 15:927-937
McManamay, R. A., D. J. Orth, C. A. Dollof, and E. A. Frimpong. 2012. Regional frameworks applied to
hydrology: can landscape-based frameworks capture the hydrologic variability? River Research
and Applications 28:1325-1339.
McManamay, R. A., D. J. Orth, C. A. Dollof, and E. A. Frimpong. 2011. A regional classification of
unregulated stream flows: spatial resolution and hierarchical frameworks. River Research and
Applications 26:1-26.
180

Alexander, K. A., J. K. Blackburn, and E. A. Frimpong. 2011. Buffalo and Maslow’s hammer. Frontiers in
Ecology and the Environment 9:302-303 + web appendix.
Peoples, B. K., and E. A. Frimpong. 2011. Among-pass, interregional, and single- versus multiple-season
comparisons of detection probabilities of stream fishes. Transactions of the American Fisheries
Society 140:67-83
Peoples, B. K., M. B. Tainer, and E. A. Frimpong. 2010. Bluehead chub nesting activity: a potential
mechanism of population persistence in degraded stream habitats. Environmental Biology of
Fishes 90:379-391
Frimpong, E. A., and P. L. Angermeier. 2010. Comparative utility of selected frameworks for regionalizing
fish-based bioassessments across the United Sates. Transactions of the American Fisheries
Society 139:1872-1895.
Frimpong, E. A., and P. L. Angermeier. 2010. Trait-based approaches in the analysis of stream fish
communities. Pages 109-136 in K. B. Gido, and D. A. Jackson, editors. Community ecology of
stream fishes: concepts, approaches, and techniques. American Fisheries Society Symposium 73,
Bethesda, Maryland.
Pritt, J. J., and E. A. Frimpong. 2010. Quantitative determination of rarity of freshwater fishes and
implications for imperiled-species designations. Conservation Biology 24:1249-1258.
Frimpong, E. A., and P. L. Angermeier. 2009. FishTraits: a database of ecological and life-history traits of
freshwater fishes of the United States. Fisheries 34:487-495.
Haas, C., E. Frimpong, and S. Karpanty. 2009. Ecosystems and Ecosystem- based management. Pages
106-142 in Innovating for Sustainable Agriculture and Natural Resources Management. SANREM
CRSP, Soil and Water Conservation Society, Ankeny.
Selected Active Grants
$206,549: National Science Foundation (Division of Environmental Biology), August 2011- September
2013- Testing the effect of context on a nest association mutualism of stream fishes in a
comparative-advantage biological market framework. Research Experience for Undergraduates
(REU) supplement included. PI
$205,250: USAID (AquaFish CRSP Associate Award- Oregon States University), October 2010-September
2013- Enhancing the profitability of small aquaculture operations in Ghana, Kenya, and Tanzania
through improved resource management and environmental Best Management Practices. PI
$197,103: USGS (Biological Informatics), August 2011-July 2013- Data consolidation and development of
an improved web-accessible public database on traits and distribution of North American
Freshwater Fishes. PI
$50,400: USGS (Aquatic GAP Analysis Program/Core Science and Analytics), July 2012- June 2013.
Preliminary application of a framework for modeling habitat suitability and distribution of stream
fishes with field-testing. PI
Professional memberships
American Fisheries Society (Parent Society & Virginia Tech Chapter),
Ecological Society of America,
Society for Conservation Biology,
Southeastern Fishes Council,
United States Aquaculture Society,
Washington Evaluators,
World Aquaculture Society
181

AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Please attach additional pages as needed.
Title of Proposal:
______________________________________________________________________________
Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
______________________________________________________________________________
For Lead Project PI (name):
Kwamena Quagrainie
______________________________________________________________________________
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending
publications and submissions
All collaborators on research projects within the past four years, including current
and planned collaborations
All thesis or postdoctoral advisees/advisors within the past four years
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
Emmanuel A. Frimpong Virginia Tech
Rebecca Lochmann University of Arkansas at Pine Bluff
Carole Engle University of Arkansas at Pine Bluff
Ganesh Kumar University of Arkansas at Pine Bluff
Maria Haws University of Hawaii at Hilo
Stephen Amisah Kwame Nkrumah university of Science and Technology
Nelson W. Agbo Kwame Nkrumah university of Science and Technology
Regina Edziyie Kwame Nkrumah university of Science and Technology
Sebastian Chenyambuga Sokoine University of Agriculture
Nazael Madalla Sokoine University of Agriculture
Hieromin A. Lamtane Sokoine University of Agriculture
Fulgence J. Mishili Sokoine University of Agriculture
Holly Wang Purdue University
Jennifer Dennis Purdue University
Paul Brown Purdue University
Robert Rode Purdue University
Steve Hart Soy in Aquaculture
Aloyce Kaliba Southern Unviersity
182

James Unterschultz University of Alberta
Michele Veeman University of Alberta
Scot Jeffrey University of Alberta
183

AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Please attach additional pages as needed.
Title of Proposal: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana
and Tanzania
______________________________________________________________________________
For Lead Project PI (name):
Kwamena Quagrainie)
______________________________________________________________________________
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending
publications and submissions
All collaborators on research projects within the past four years, including current
and planned collaborations
All thesis or postdoctoral advisees/advisors within the past four years
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
Please complete a separate list for each HC PI and subcontracting PIs (not necessary for co-PIs)
HC or subcontracting PI (name):
Rebecca Lochmann
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending publications
and submissions
Todd Sink, Texas A&M University
Delbert M. Gatlin, III, Texas A&M University
Menghe Li, Mississippi State
Allen Davis, Auburn University
Jesse Trushenski, Southern Illinois University, Carbondale
Steven Rawles, USDA/ARS/SNARC
Craig Tucker, USDA/ARS/Catfish Genetics Unit/Stoneville, MS
Jim Avery, Director, Southern Regional Aquaculture Center, MS
Carole Engle, UAPB
Brian Bosworth, Mississippi State University
184

Menghe Li, Mississippi State University
Andrew Proctor, University of Arkansas - Fayetteville
Shahidul Islam, UAPB
All collaborators on research projects within the past four years, including current and
planned collaborations
Todd Sink, Texas A&M University
Delbert M. Gatlin, III, Texas A&M University
Menghe Li, Mississippi State
Allen Davis, Auburn University
Jesse Trushenski, Southern Illinois University, Carbondale
Steven Rawles, USDA/ARS/SNARC
Craig Tucker, USDA/ARS/Catfish Genetics Unit/Stoneville, MS
Jim Avery, Director, Southern Regional Aquaculture Center, MS
Carole Engle, UAPB
Brian Bosworth, Mississippi State University
Menghe Li, Mississippi State University
Andrew Proctor, University of Arkansas - Fayetteville
Shahidul Islam, UAPB
Rodrigo Ozorio, CIIMAR, Portugal
All thesis or postdoctoral advisees/advisors within the past four years
Michele Thompson
Tom Kasiga
Andrew Maina
Jimmy Faukner
Yathish Ramena
Baby Suja
Miles Thompson
Daryl Weldon
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
040113
None
185

AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Please attach additional pages as needed.
Title of Proposal: Aquaculture Production and Human Health, Nutrition, and Food Supply in
Ghana and Tanzania
For U.S. Co-PI
Maria Haws, University of Hawaii Hilo
Last name First name Organization
Anderson Glen IRG/Engility
Aviti Mmochi IMS
Balladares Eufrasia
Juan
UCA
Bravo Ramon UCA
Camacho Lorna UAS
Castro Kathy URI
Classen Stephan UHH
Cordero Francisco CIAD
Crawford Brian URI
Ellis Simon UHH
Fong Quentin Univ. of Alaska
Francis Julius WIOMSA
Garcia Ortega Armando UHH
Garriques Daren UHH
Gaxiola Eladio UAS
Hernandez Nelvia UCA
Hopkin Kevin UHH
Howerton Robert UH
Jean Pierre Michaud UHH
Jiddawe Narriman IMS
Kim Yoon IRG/Engility
LeClaire Carlos UCA
Leung Pingsun LSU
McDermind Karla UHH
Merrifield Mark UHM
Mickelson Aaron UHH
Monserrat Miguel UCA
Msuya Flower IMS
Nisbet David Goosepoint Oyster
Paynter Kennedy Univ. of Maryland
Rodriguez Gustavo UAS
Rodriguez Guillermo UAS
Ron Benny UHM
Singeo Singeru Land Grant
186

Supan John LSU
Torrel Elin URI
187

AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Please attach additional pages as needed.
Title of Proposal: Aquaculture Production and Human Health, Nutrition and Food Supply in
Ghana and Tanzania
For Lead Project PI (name):
Kwamena Quagrainie
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending
publications and submissions
All collaborators on research projects within the past four years, including current
and planned collaborations
All thesis or postdoctoral advisees/advisors within the past four years
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
Please complete a separate list for each HC PI and subcontracting PIs (not necessary for co-PIs)
HC or subcontracting PI (name):
Stephen Amisah, PhD
All co-authors on publications within the past four years, including pending publications
and submissions
Dr. Emmanuel Frimpong, Dr. Daniel Adjei Boateng, Dr Kwamena Quagrainie
All collaborators on research projects within the past four years, including current and
planned collaborations:
Purdue University, West Lafayette, Virginia Tech and State University, Blacksburg
All thesis or postdoctoral advisees/advisors within the past four years: Thesis Research
Advisees:
Mr. Kwame Akpaglo, MSc thesis, Mr. Karikari (WRI) PhD res
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years: Nil
188

AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Please attach additional pages as needed.
Title of Proposal: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
For Lead Project PI (name):
Kwamena Quagrainie
____________________________________________________________________________
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending
publications and submissions
All collaborators on research projects within the past four years, including current
and planned collaborations
All thesis or postdoctoral advisees/advisors within the past four years
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
For HC PI
Name: Sebastian Chenyambuga
All co-authors on publications within the past four years, including pending
publications and submissions
Name Address
C. Waiswa Department of Veterinary Medicine, Makerere University, P.O Box
7062 Kampala, Uganda
M. Saimo Department of Veterinary Medicine, Makerere University, P.O Box
7062 Kampala, Uganda
P. Ngumi Kenya Agricultural Research Institute (KARI), Muguga, P.O.Box
30148, Nairobi, Kenya
P. S. Gwakisa Department of Veterinary Microbiology and Parasitology, Sokoine
University of Agriculture, P.O.Box 3015, Morogoro, Tanzania
M.M. Kibodya Department of Animal Science and Production, Sokoine University
of Agriculture, P.O.Box 3004, Morogoro, Tanzania
B.V. Mnembuka Department of Animal Science and Production, Sokoine University
of Agriculture, P.O.Box 3004, Morogoro, Tanzania
N.A. Madalla Department of Animal Science and Production, Sokoine University
of Agriculture, P.O.Box 3004, Morogoro, Tanzania
E. D. Karimuribo Department of Veterinary Medicine and Public Health,
Sokoine University of Agriculture (SUA), P.O. Box 3021
Morogoro, Tanzania
189

V. W. Makene The Open University of Tanzania (OUT), Iringa Regional
Centre, P.O. Box 23409 Dar-es-Salaam, Tanzania
S. Mathias Dar es Salaam Institute of Technology (DIT), P.O. Box 2958
Dar es Salaam, Tanzania
E. M. Eblate Tanzania Wildlife Research Institute, P.O. Box 661, Arusha,
Tanzania
K. J. Lughano Department of Veterinary Medicine and Public Health,
Sokoine University of Agriculture (SUA), P.O. Box 3021
Morogoro, Tanzania
P.L. Msoffe Department of Veterinary Medicine and Public Health,
Sokoine University of Agriculture (SUA), P.O. Box 3021
Morogoro, Tanzania
R. H. Knut Department of Basic Sciences and Aquatic Medicine,
Norwegian School of Veterinary Science, N-0033, Oslo,
Norway
D.M. Komwihangilo National Livestock Research Institute, P.O. Box 202
Mpwapwa, Tanzania
M. Jackson National Livestock Research Institute, P.O. Box 202
Mpwapwa, Tanzania
S. Bitanyi Ministry of Livestock Development and Fisheries -
Veterinary Investigation Centre -
Temeke, P.O Box 9254 Dar-Es-Salaam, Tanzania
M. Nesje Department of Production Animal Clinical Science, Norwegian
School of Veterinary
Science, PO Box 8146 Dep., N – 0033 Oslo, Norway
B.P. Kaltenborn Norwegian Institute for Nature Research (NINA), Fakkelgården,
Storhove, N-2624
Lillehammer, Norway.
P.L. Mwambene Livestock Research Centre Uyole,
PO Box 6191, Mbeya, Tanzania.
A.M. Katule Department of Animal Science and Production, Sokoine University
of Agriculture, P.O.Box 3004, Morogoro, Tanzania
P.A.A. Mwakilembe Livestock Research Centre Uyole,
PO Box 6191, Mbeya, Tanzania.
Y. Plante Canadian Animal Genetic Resources Program, Agriculture and
Agri-Food Canada, 107 Science Place Saskatoon, Saskatchewan,
Canada
All collaborators on research projects within the past four years, including current
and planned collaborations
Name Address
Kwamena
Purdue University, College of Agriculture, Krannert building, 403
Quagrainie
W. State Street, West Lafayette, 1N 47907-2056, USA.
Rebecca Lochmann Aquaculture and Fisheries Center, University of Arkansas at Pine
Bluff, 1200 N. University Dr. Mail slot 4912, Pine Bluff, AR 71601,
USA.
190

Ian Mayer Norwegian School of Veterinary Science (NVH), N-0033 Oslo,
Norway.
Ingrid Olesen Norwegian Institute of Food, Fisheries and Aquaculture Research
(Nofima), N-1432 Aas. Norway.
Kurt Buchmann University of Copenhagen, Faculty of Health and Medical Sciences,
Stigbøjlen 7, DK-1870 Frederiksberg C, Denmark
Lene Møller Madsen Department of Science Education, University of Copenhagen,
Østre Voldgade 3
DK-1350 Copenhagen, Denmark
Eli Katunguka Makerere University, P.O. Box 7062, Kampala, Uganda
Rwakishaya
All thesis or postdoctoral advisees/advisors within the past four years
Name Address
Y. Msanga Ministry of Livestock and Fisheries Development, P Mandela
Road, Mifugo House, P. O. Box 9152,
Tanzania
S.M. Das
Central Veterinary Laboratory,
Nelson Mandela Road, Temeke,
P.O.Box 9254, Dar es Salaam, Tanzania
D. M.
Komwihangilo
National Livestock Research Institute, P.O. Box 202
Mpwapwa, Tanzania
Sendalo D.R. National Livestock Research Institute, P.O. Box 202
Mpwapwa, Tanzania
Raphael G. University of Nairobi, P.O. Box 30197 – 001000, Nairobi, Kenya.
Wahome
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
None
191

AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Please attach additional pages as needed.
Title of Proposal: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
For Lead Project PI (name):
Kwamena Quagrainie
____________________________________________________________________________
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending
publications and submissions
All collaborators on research projects within the past four years, including current
and planned collaborations
All thesis or postdoctoral advisees/advisors within the past four years
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
For Co-Host Country PI
Name: Nazael Amos Madalla
All co-authors on publications within the past four years, including pending
publications and submissions
Name Address
M.M. Kibodya Department of Animal Science and Production,
Sokoine University of Agriculture, P.O.Box 3004,
Morogoro, Tanzania
B.V. Mnembuka Department of Animal Science and Production,
Sokoine University of Agriculture, P.O.Box 3004,
Morogoro, Tanzania
S. W. Chenyambuga Department of Animal Science and Production,
Sokoine University of Agriculture, P.O.Box 3004,
Morogoro, Tanzania
H. A. Lamtane Department of Animal Science and Production,
Sokoine University of Agriculture, P.O.Box 3004,
Morogoro, Tanzania
N. W. Agbo Department of Fisheries and Watershed
Management, Kwame Nkrumah University of
192

Science and Technology
K. Jauncey Institute of Aquaculture, University of Stirling,
FK9 4LA, United Kingdom
P. Sorgeloos Laboratory of Aquaculture & Artemia Reference
Center, Faculty of Bioscience Engineering -
Department of Animal Production
Ghent University
M. Wille Laboratory of Aquaculture & Artemia Reference
Center, Faculty of Bioscience Engineering -
Department of Animal Production
Ghent University
All collaborators on research projects within the past four years, including current
and planned collaborations
Name Address
Kwamena Quagrainie Purdue University, College of Agriculture,
Krannert building, 403 W. State Street, West
Lafayette, 1N 47907-2056, USA.
Rebecca Lochmann Aquaculture and Fisheries Center, University of
Arkansas at Pine Bluff, 1200 N. University Dr.
Mail slot 4912, Pine Bluff, AR 71601, USA.
Ian Mayer Norwegian School of Veterinary Science (NVH),
N-0033 Oslo, Norway.
Ingrid Olesen Norwegian Institute of Food, Fisheries and
Aquaculture Research (Nofima), N-1432 Aas.
Norway.
Kurt Buchmann University of Copenhagen, Faculty of Health
and Medical Sciences, Stigbøjlen 7, DK-1870
Frederiksberg C, Denmark
Lene Møller Madsen Department of Science Education, University of
Copenhagen, Østre Voldgade 3
DK-1350 Copenhagen, Denmark
All thesis or postdoctoral advisees/advisors within the past four years
Name Address
K. Jauncey Institute of Aquaculture, University of Stirling,
FK9 4LA, United Kingdom
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
None
193

AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Please attach additional pages as needed.
Title of Proposal: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana
and Tanzania
______________________________________________________________________________
For Lead Project PI (name):
Kwamena Quagrainie)
______________________________________________________________________________
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending
publications and submissions
All collaborators on research projects within the past four years, including current
and planned collaborations
All thesis or postdoctoral advisees/advisors within the past four years
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
Please complete a separate list for each HC PI and subcontracting PIs (not necessary for co-PIs)
HC or subcontracting PI (name):
Regina Edziyie
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending publications
and submissions
Peter Perschbacher, University of Arkansas at Pine Bluff
Gerald Ludwig, USDA/ARS/ Stuggart, AR
Gopinath Nallani, University of North Texas
Peter Paulos, University of North Texas
Lisa Constatine, Pfizer/Environmental Science/Pharmacokinetics, Groton, CT.
Duane Huggett, University of North Texas
All collaborators on research projects within the past four years, including current and
planned collaborations
194

Peter Perschbacher, University of Arkansas at Pine Bluff
Gerald Ludwig, USDA/ARS/ Stuggart, AR
Gopinath Nallani, University of North Texas
Peter Paulos, University of North Texas
Lisa Constatine, Pfizer/Environmental Science/Pharmacokinetics, Groton, CT.
Duane Huggett, University of North Texas
Barney Venebles, University of North Texas
Ruthanne Thompson, University of North Texas
Thomas LaPoint, University of North Texas
Daniel Adjei Boateng, Kwame Nkrumah University of Science & Technology
All thesis or postdoctoral advisees/advisors within the past four years
Thomas LaPoint
Allen Burton, Jr.
Duane Huggett
Barney Venebles
Paul Hudak
Ruthanne Thompson
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
040113
None
195

AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Please attach additional pages as needed.
Title of Proposal: Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana
and Tanzania
______________________________________________________________________________
For Lead Project PI (name):
Kwamena Quagrainie)
______________________________________________________________________________
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending
publications and submissions
All collaborators on research projects within the past four years, including current
and planned collaborations
All thesis or postdoctoral advisees/advisors within the past four years
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
Please complete a separate list for each HC PI and subcontracting PIs (not necessary for co-PIs)
HC or subcontracting PI (name):
Nelson Winston Agbo
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending publications
and submissions
Steve Amisah, Kwame Nkrumah University of Science & Technology
Daniel Adjei-Boateng, Kwame Nkrumah University of Science & Technology
Kim Jauncey, Stirling University, UK
Nazael Madalla, Sokoine University of Agriculture, Tanzania
Emmanuel A. Frimpong, Virginia Polytechnic Institute and State University, Blacksburg
Gifty Anane-Taabeah, Virginia Polytechnic Institute and State University, Blacksburg
Kwamena Quagrainie, Purdue University
Richard Buamah, Kwame Nkrumah University of Science & Technology
Ashley Murray, International Water Management Institute
196

All collaborators on research projects within the past four years, including current and
planned collaborations
Steve Amisah, Kwame Nkrumah University of Science & Technology
Daniel Adjei-Boateng, Kwame Nkrumah University of Science & Technology
Emmanuel A. Frimpong, Virginia Polytechnic Institute and State University, Blacksburg
Kwamena Quagrainie, Purdue University
Ashley Murray, International Water Management Institute
William Leschen, Stirling University, UK
All thesis or postdoctoral advisees/advisors within the past four years
Kim Jauncey
Randolph H. Richards
Peter Akpaglo
Gjampo Benjamin
Kwasi Obirikorang
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
None
040113
197

Title of Proposal:
AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Please attach additional pages as needed.
Aquaculture Production and Human Health, Nutrition, and Food Supply in Ghana and
Tanzania
For Lead Project PI (name):
Kwamena K. Quagrainie
__________________________________________________________________________
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending
publications and submissions
All collaborators on research projects within the past four years, including current
and planned collaborations
All thesis or postdoctoral advisees/advisors within the past four years
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
Please complete a separate list for each HC PI and subcontracting PIs (not necessary for co-PIs)
HC or subcontracting PI (name):
Emmanuel A. Frimpong
The following information is needed to establish instances where a conflict-of-interest may exist
between PIs and prospective technical peer-reviewers and external program evaluators. For
each PI (Project Lead and other US & HC PI) please provide a list with the names of the
following people and associated institutions or organizations.
All co-authors on publications within the past four years, including pending
publications and submissions
All collaborators on research projects within the past four years, including current
and planned collaborations
All thesis or postdoctoral advisees/advisors within the past four years
All persons in your field with whom you have had a consulting/financial
arrangement/other conflict-of-interest within the past four years
040113
Collaborations and Other Affiliations
Coauthors
Agbo, Nelson Nkrumah University of Science and Technology, Ghana
198

Amisah, Steve Kwame Nkrumah University of Science and Technology, Ghana
Anane-Taabeah, Gifty Kwame Nkrumah University of Science and Technology, Ghana
Ansah, Yaw Virginia Polytechnic Institute & State University
Dicenzo, Vic Virginia Polytechnic Institute & State University
Delince, Guy Independent Aquaculture expert, Brussels, Belgium
Dollof, C. Andrew USDA Forest Service
Deweber, Jefferson Pennsylvania State University
Haas, Carola Virginia Polytechnic Institute & State University
Henebry, Michael Purdue University
Jiao, Yan Virginia Polytechnic Institute & State University
McManamay, Ryan Oakridge National Laboratory
Murphy, Brian Virginia Polytechnic Institute & State University
Namara, Regassa International Water Management Institute
Orth, Donald Virginia Polytechnic Institute & State University
Pendleton, Richard Illinois DNR
Peoples, Brandon Virginia Polytechnic Institute & State University
Pritt, Jeremy University of Toledo
Stich, Dan University of Maine
Tainer, Molly Virginia Polytechnic Institute & State University
Tettey, Ethel Kwame Nkrumah University of Science and Technology, Ghana
Collaborators
Alexander, Kathleen Virginia Polytechnic Institute & State University
Angermeier, Paul USGS Co-op Unit, Virginia Polytechnic Institute & State University
Gyakum, John McGill University, Canada
Infante, Dana Michigan State University
Orth, Donald Virginia Polytechnic Institute & State University
Ostroff, Andrea USGS, Aquatic Gap Analysis/Core Science & Analytics
Norton, George Virginia Polytechnic Institute & State University
Quagrainie, Kwamena Purdue University
Xie, Zhiwu Virginia Polytechnic Institute & State University
Graduate and Post-Doctoral Advisors
Paul L. Angermeier Virginia Polytechnic Institute & State University PostdocAdvisor
Dennis C. Le Master Purdue University (Retired), PhD Advisor
Trent M. Sutton University of Alaska at Fairbanks PhD co-Advisor
Steve E. Lochmann University of Arkansas at Pine Bluff MS Advisor
Thesis & Dissertation Advisees
Anane-Taabeah, Gifty – completed MS
Ansah, Yaw B. – completed MS; Current Doctoral
Buckwalter, Joseph – Current MS
Deweber, Jefferson T. – completed MS
Floyd Stephen – Current MS
Fynn, Iris – Current MS
Henebry, M. Lee – completed MS
Huang, Jian – Current Doctoral
Peoples, Brandon K. – completed MS; Current Doctoral
Pritt, Jeremy J. – completed MS
Watkins Steven – Current MS
Liang, Yu – Current MS/GIS Specialist
040113
199

AQUAFISH INNOVATION LAB PROPOSAL
CONFLICT-OF-INTEREST FORM
Please attach additional pages as needed.
Title of Proposal: Aquaculture Production and Human Health, Nutrition, and Food Supply in
Ghana and Tanzania
Last name First name Organization
Aviti Mmochi IMS
Dalhousie
Chircop Aldo University
University of
Carneiro, G. Lisbon
Crawford Brian URI
Ohio State
Dabrowski Konrad University
Ellis Simon UHH
Dominguez Guillermo UAS
Gaxiola Eladio UAS
University of
Grilo Catarina Lisbon
University of
Guerreiro Jose Lisbon
Haws Maria UHH
Hernandez Nelvia UCA
Nicolaus
Jaroszewska Marta Copernicus Univ.
Jiddawi Narriman IMS
LeClaire Carlos UCA
Mahongo Shigalla TAFIRI
Pacule Hermes CEPAM
São Paulo
Portella Maria State University
Ricci Glenn URI
Rodriguez Gustavo UAS
Sandoval Erick UCA
Sisitka Lawrence Rhodes University
Squillante Lesley URI
Tobey James URI
Torrel Elin URI
Van Der Elst Rudy ORI
For U.S. Co-PI
Julius Francis, WIOMSA
200

AQUAFISH INNOVATION LAB PROPOSAL
PURDUE UNIVERSITY PROJECT
LIST OF REVIEWERS
NAME CONTACT INFORMATION AREA OF EXPERTISE
Dr. Diego
Valderrama
Dr. Kennedy
Paynter
Dr. Christopher
Green
University of Florida
Office: 352-294-7678
dvalderrama@ufl.edu
University of Maryland
Office: 301-405-7684
Lab: 410-326-7259
paynter@mees.umd.edu
Louisiana State University
Office: 225-578-4131
CGreen@agcenter.lsu.edu
Dr. Brian Small Southern Illinois University
Carbondale
Office: 618-453-6015
bcsmall@siu.edu
Dr. Laura G. Tiu Ohio State University South
Centers
Office: 740-289-2071
tiu.2@osu.edu
201
Fisheries and Aquaculture
Economics; Economic
Development
Oyster biology and aquaculture
Applied Reproductive Fish
Physiology and Endocrinology
Fish physiology, Endocrinology
and Nutrition
Human and Community
Resource Development in
Aquaculture