Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
the Ova-specific T cells were producing IFNγ after i.v. injection; whereas 50-70% of the cells
were effector CD8 + T cells at the peak of the i.d. response (Figure 24B). Representative
FACS plots highlight that not only did we achieve a higher percentage of IFNγ-producing
cells with i.d. injection, but also, on a per cell basis, many of the effector T cells were making
10-fold more cytokine as compared to those isolated after i.v. immunization (Figure 24C).
This was also evident using a population-based analysis – as shown, the geometric mean
fluorescent intensity (MFI) of tetramer-positive cells was significantly higher in the i.d.
condition on days 9-15 (Figure 24D). While intradermal immunization is delayed, it cross-
primes CD8 + T cells with greater effector function.
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