Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
Figure 22. The requirements for efficient cross-priming may be skewed by high T cell precursor
frequency. 10 3 or 10 6 CD45.1 OT-I splenocytes were transferred into CD45.2 recipients prior to
immunization. Use of congenic markers allowed simultaneous assessment of transferred and
endogenous Ova-specific T cells (schematic representation). Mice were immunized with 5x10 5
K bm1 mOva splenocytes. On day 5 post-immunization, enrichment was performed using both K b -
SIINFEKL tetramer and CD45.1 antibody to distinguish endogenous tetramer-positive cells and OT-I.
Live CD3 + CD8 + DUMP - cells are shown. The upper region highlights the transferred OT-I and the
lower region marks the endogenous Ova-reactiva CD8 + T cells. Absolute cell numbers are indicated
for the respective cell populations. Plots were selected from an experiment with three mice per group.
Data are representative of three independent experiments.
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