Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
OT-I T cells and endogenous Ova-specific T cells. As shown, only the i.v. immunization
resulted in the early priming of Ova-specific T cells. Representative plots are shown,
indicating that both the OT-I and the endogenous T cells behaved similarly, and that
responses were comparable to those observed in animals that had not received OT-I (Figure
22). Analysis of later time points supported the conclusion that priming is delayed when mice
are immunized via the i.d. route (data not shown). Furthermore, we demonstrated that T cell
precursor frequency influences the kinetics of priming. Transfer of 10 6 OT-I prior to
immunization, in contrast to low transfer conditions, resulted in the robust and rapid
expansion of Ova-specific T cells in both i.v. and i.d. conditions (Figure 22). Also evident,
the transferred cells outcompeted the endogenous repertoire. These data indicate that there
exists a qualitative difference between i.v. and i.d. immunization, which is masked when
using adoptive transfer of high numbers of monoclonal T cells. This highlights also the
necessity of new techniques such as tetramer-based enrichment to address this kind of
questions.
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