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Voie d'immunisation et séquence d'administration de l ... - TEL

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tel-00827710, version 1 - 29 May 2013<br />

Figure 20. Combination of t<strong>et</strong>ramer-based enrichment with intracellular staining. (A) The 2<br />

protocols were <strong>de</strong>veloped as <strong>de</strong>scribed. (B) C57BL/6 mice were immunized i.d. with 5x10 5 K bm1 mOva<br />

splenocytes. On day 12, spleen and lymph no<strong>de</strong>s were harvested and an enrichment combined with<br />

intracellular staining for IFNγ (protocol 1) was performed. CD8 + T cells are shown.<br />

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