Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
splenocytes are targets for NK cells, as they completely lack MHC-I molecule surface
expression, this is not the case for H-2K bm1 splenocytes on which the defective MHC is still
expressed (Figure 16C). To note, the K bm1 molecule differs from the K b molecule by seven
nucleotides, resulting in only three different amino acids differences (Schulze et al., 1983).
Figure 16. Characterization of K bm1 mOva splenocytes. (A) WT, K b-/- or K bm1 mOva splenocytes
were stained for K b using a monoclonal antibody, clone AF6-88.5. (B) The same splenocytes were
incubated with SIINFEKL peptide for 1 hour and then whashed and stained with an anti-K b -
SIINFEKL antibody. (C) C57BL/6 mice were injected with anti-NK1.1 antibody or the isotype control
the day prior to, and the day of immunization. Mice were immunized with a mixture of β2m -/- , K bm1
and WT Ova-expressing splenocytes labeled with 3 different concentrations of CFSE. 16 hours postimmunization,
the spleen was harvested and the proportions of injected cells still present was
evaluated. (C) This experiment was performed by H. Saklani.
Using this model, we were first interested in examining the impact of the route of
immunization on CD8 + T cell cross-priming. Several injection routes have been used in a
variety of experimental research models and, when we compared datas based on the different
routes of injection, we observed some striking differences in the induction of effective
immune responses. For example, the injection of male splenocytes, depleted for CD11c + cells
to avoid direct presentation, into female recipients was able to trigger efficient cross-priming
directed against male antigen only if they were delivered i.d.; no response was observed if the
cells were injected i.v. (Figure 17).
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