Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
response, despite the substantial evidence that the optimal use of these components will
significantly boost the immune response (Wilson et al., 2006).
In this section, I have reviewed the many parameters that can influence the outcome of an
immune response. These factors must all be carefully considered and examined when
developing new vaccines. To efficiently address this, models that represent physiologic
conditions in humans are needed, as well as the development of technologies and tools that
will allow for the detailed examination of a physiological immune response, even at low
levels. Indeed, as currently studied, some parameters that influence the extent of an immune
response, such as adjuvant dose, T cell precursor frequency and route of administration are
artificially modified in mouse models. While this can facilitate the study of the immune
response and limit the variability in the results, it was just reviewed here how these
manipulations can impact the outcome of the response. Thus, it is imperative to keep these
parameters as close as possible to human physiology. In order to study the immune response
in these “physiologic” conditions, new techniques are continually being developed, such as
the tetramer-based enrichment assay. As each technique presents its own limitations, the
difficulty lies in choosing the compromise between the physiologic relevance of the model
and the ability to study the T cell response with the tools available.
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