Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
A
B
Fig.5. Gating strategy and Enrichment. (A) Example of the gating strategy applied to an Influenza A-Matrix 158-66
enriched human sample. SSC-A low /SSC-W low initial gating permit to exclude doublets, then Dump vs. CD3 contour
plot permits to isolate viable pure CD3 for further analysis. Upper line shows evaluation of background on a
Multimer-PE vs CD8 contour plot still gated on the total CD3 population. Bottom line illustrates CD3 + CD8 +
selection, then finally evaluation of Multimer-PE + cells percentages within CD8 + T cells. (B) Left plots: PBMCs
from a healthy donor have been incubated with CMV, Flu or HCV MHCI multimers, then enriched as described in
the protocol. Right plots : C57BL/6 mice were immunized intradermally, either with male (HY model) or
K bm1 mOva (Ova model) splenocytes. On day 11, the spleen and lymph nodes were harvested and enrichment was
performed as described in the protocol, using D b -UTY (HY model) or K b -SIINFEKL (Ova model) multimers.
232