Voie d'immunisation et séquence d'administration de l ... - TEL
Voie d'immunisation et séquence d'administration de l ... - TEL Voie d'immunisation et séquence d'administration de l ... - TEL
tel-00827710, version 1 - 29 May 2013 Fig.3. Flow cytometry based-HLA typing (Human). PBMCs are prepared as usual, then incubated with either isotype or anti-HLA antibody of interest titrated to the optimal concentration. Histograms represent data obtained from one HLA-A2 positive (left) and one HLA-A2 negative (right) blood donors. 230
tel-00827710, version 1 - 29 May 2013 Fig.4. Representative example of single and double multimer staining. PBMCs from one healthy donor were stained with Influenza A-Matrix158-66 MHCI multimer labeled either in PE (left), APC (middle), or both (right). Plots are gated into global CD8 + population using the gating strategy described in Section 3.5-Figure 5A. Page 231 of 256
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tel-00827710, version 1 - 29 May 2013<br />
Fig.4. Representative example of single and double multimer staining. PBMCs from one healthy donor were<br />
stained with Influenza A-Matrix158-66 MHCI multimer labeled either in PE (left), APC (middle), or both<br />
(right). Plots are gated into global CD8 + population using the gating strategy <strong>de</strong>scribed in Section 3.5-Figure<br />
5A.<br />
Page 231 of 256
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