Voie d'immunisation et séquence d'administration de l ... - TEL
Voie d'immunisation et séquence d'administration de l ... - TEL Voie d'immunisation et séquence d'administration de l ... - TEL
tel-00827710, version 1 - 29 May 2013 Fig.1. Schematic representation of purified biotinylated MHC Class I molecule (left) and multimer (right) (adapted from Klenerman et al. (9)). 228
tel-00827710, version 1 - 29 May 2013 Fig.2. Schematic representation of the enrichment procedure. Starting from cellular suspension, cells are stained with PE-labeled multimer, then incubated with antiPE-microbeads before loading on a MACS column. Flow through is the Depleted fraction. By removing the column, you then have access to your Enriched fraction, containing increased numbers of multimer-positive cells. Page 229 of 256
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tel-00827710, version 1 - 29 May 2013<br />
Fig.2. Schematic representation of the enrichment procedure. Starting from cellular suspension, cells are stained<br />
with PE-labeled multimer, then incubated with antiPE-microbeads before loading on a MACS column. Flow<br />
through is the Depl<strong>et</strong>ed fraction. By removing the column, you then have access to your Enriched fraction,<br />
containing increased numbers of multimer-positive cells.<br />
Page 229 of 256