Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
1. Introduction
Mature CD8 + T lymphocytes bear αβ T cell receptors (TCR) that are specific for a
major histocompatibility complex (MHC) class I molecule bound to a unique peptide. A
major goal in the study of adaptive immune responses is to understand the developmental
progression of antigen-specific T cells from naive precursors to activated effector cells and
long-lived memory cells [1,2]. Prior to 1996, limiting dilution analysis was the standard
method for estimating the frequency of antigen specific T cells. The major limitation of this
approach is the requirement for exogenous stimulation and expansion, introducing potential
bias and significant inter-assay variability. Notably, cloning efficiency is typically