Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
I. MICE
A. Mouse strains
Table 7. Mouse strains used
B. Bone-Marrow chimeras
6 week-old mice to be used as BM-chimeras hosts were irradiated (Cesium source). The
appropriate duration of treatment was calculated based on the time necessary to receive an
irradiation dose of 1250rad. Mice used as bone marrow donors were all less than 6 months of
age. Donor mice were euthanized and bones were harvested. Bone marrow cell suspensions
were prepared in PBS and at least 5 million total leukocytes were transferred i.v. per mouse.
Six weeks later, the extent of chimerism was determined in the blood by evaluating the
staining for the congenic markers CD45.1 and CD45.2 by flow cytometry.
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