Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
Figure 57. Combination and sequence of administration of different approaches for cancer
therapy. From the known actions of the different therapeutic agents, we propose a model to choose
the optimal timing of delivery. Arrows and lines represent the optimal time window for each treatment
depending on the step it is supposed to act.
Although, we chose to describe how to apply our data and model to the development of anti-
cancer therapy, the same strategies may be applied to other diseases where different
approaches and combinations might be required to optimize current patient treatment.
IV. CONCLUSION
In conclusion, we have used a model of cell-associated antigen to perform a thorough, careful
anaysis of the CD8 + T cell response after cross-presentation. The development of the
tetramer-based enrichment strategy and its combination with other approaches such as
intracellular cytokine staining or immunoscope allowed us to perform an in-depth study of the
kinetics, phenotype and functionality of the endogenous CD8 + T cell response.
We applied these strategies to study first the impact of the route of immunization on CD8 + T
cell cross-priming. We compared the efficiency and effectiveness of local versus systemic
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