Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
Figure 50. The differential effects of adjuvant impact protection against Listeria. Mice were
immunized i.d. with 5x10 5 K bm1 mOva splenocytes. Poly I:C was administered either the day of
immunization or 3 days later. On day 9 post-immunization, mice were challenged with 5x10 5 CFU of
Ova-expressing Listeria. Two days later, the spleen (A) and the liver (B) were harvested and bacterial
load per organ was determined. NI, non-immunized mice. Dotted lines correspond to median CFU in
the absence of poly I:C. Mann-Whitney test p-values were calculated, comparing immunization
condition to the NI control.
In this chapter, we have demonstrated that the optimal timing for adjuvant delivery is
critically dependent on the route of immunization. Our data strongly suggest that this effect is
due to the different kinetics of antigen uptake and presentation upon i.d. or i.v. antigen
delivery. Specifically, the timing of antigen capture and T cell engagement has a profound
impact on the appropriate timing for adjuvant treatment. For optimal vaccination, there is
interest and need to optimize the coordination between innate and adaptive immune
responses; our data suggest that a careful optimization of adjuvant and antigen administration
will be required.
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