Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
den Haan et al., 2000). Following poly I:C injection, we observed a striking decrease in the
total number of splenic CD8α + DCs (Figure 43A). Analysis of the remaining cells indicated
that activation markers CD86 and MHC-II are upregulated within 15h post-injection,
indicating that maturation is a rapid process (Figure 43B). In contrast to the spleen, DC
numbers in lymph nodes increased after poly I:C injection; and again the cells demonstrated a
mature phenotype within 1 day of poly I:C administration (Figure 43B). Clearly, poly I:C
impacted survival and/or migration of DCs in the lymphoid organs. This effect may be
responsible for the inhibition of cross-priming if it results in the loss of DCs available at the
site of injection, prepared to take up antigen. This rapid DC maturation could also contribute
to the inhibition of subsequent priming by downregulating antigen engulfment, which is a
characteristic feature of immature DCs.
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