Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
To confirm that early delivery of adjuvant inhibited priming due to a failure to capture and
present cell-associated antigen, we utilized the adoptively transferred CFSE-labeled OT-I
system. Administration of poly I:C one day after i.d. immunization completely blocked OT-I
proliferation and IFNγ production (Figure 38). If instead we waited until three days post-
immunization to administer the poly I:C, this blockade was no longer observed and, in fact, a
greater percentage of OT-I showed maximal cell division and effector function (Figure 38,
arrow). Based on these results, we initiated a study to further detail the mechanisms
underlying these differential effects of adjuvant on cross-priming efficacy depending on the
timing of adjuvant delivery.
Figure 38. Opposite effects of adjuvant depending on the timing of administration. Mice were
immunized i.d. with 5x10 5 K bm1 mOva splenocytes. On day 1 or day 3 post-immunization, 50µg of
poly I:C or PBS was injected i.v. On day 3 post-immunization, 5x10 6 CD45.1 + CFSE-labeled OT-I
splenocytes were transferred i.v. Three days later the draining lymph node was harvested and the
dilution of CFSE staining of OT-I was determined, represented by histograms. Intracellular staining
for IFNγ was performed at the same time, shown in the corresponding FACS plots. CD3 + CD8 +
CD45.1 + cells were gated for the analysis shown. Data are representative of three independent
experiments. NI, non-immunized mice.
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