Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
Figure 37. Optimal timing of adjuvant delivery is dependent on the route of immunization. (A,B)
Mice were immunized i.d. or i.v. with 5x10 5 K bm1 mOva splenocytes and received 100µg of poly I:C at
the indicated time points. For mice immunized i.v., they received poly I:C i.v. either: 1 day before
immunization, the day of immunization combined with antigen, 5h, or 1 day post-immunization. The
spleen and 15 macroscopic lymph nodes were harvested on day 7, which corresponds to the peak of
the CD8 + T cell response. K b -SIINFEKL tetramer-based enrichment was performed and the absolute
numbers of tetramer-positive CD8 + T cells is reported (A). For mice immunized i.d., they received
poly I:C at the same time points and one additional group was added, 3 days post-immunization. Poly
I:C was administered i.v., except for the mice injected on day 0 with poly I:C formulated with the
antigen. Analysis was performed on day 9 post-immunization, again corresponding with peak CD8 + T
cell response (B). p-values were calculated using a Mann-Whitney test, comparing in a two-way test,
adjuvant condition to no poly I:C treatment. Dotted lines correspond to the median number of
responding cells in the absence of poly I:C. NI, non-immunized mice are shown to indicate baseline
responses.
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