Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
Figure 30. Single mouse analysis confirms that route of immunization does not influence T cell
diversity. Mice were immunized i.d. (A) or i.v. (B) with 5x10 5 K bm1 mOva splenocytes. On day 9, 15
macroscopic lymph nodes and the spleen were harvested and a K b -SIINFEKL tetramer-based
enrichment was performed for each mouse. CD8 + K b -SIINFEKL tetramer-positive cells were sorted.
Immunoscope was performed on cells sorted from individual mice (3,000- 5,000 cells sorted per
mouse) to define the length of the CDR3 loop in the 24 Vβ families. (A, B) Each color represents a
distinct Vβ family. (C) The total number of peaks detected in all Vβ profiles was enumerated and
represented. Data points indicate a single mouse. Statistical analysis comparing ID and IV was
assessed by Mann-Whitney test. NI, non-immunized mice, shown to indicate baseline diversity of
TCR.
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