Voie d'immunisation et séquence d'administration de l ... - TEL

tel-00827710, version 1 - 29 May 2013
data in an infectious model. Mice were immunized i.d. or i.v. with K bm1 mOva splenocytes and
challenged 1 month later with Ova-expressing Listeria monocytogenes. Unfortunately we
were not able to repeat the data obtained previously in the rechallenge with splenocytes (data
not shown). This is most likely due to relatively small differences between the two routes of
immunization as compared to the strength of Listeria infection.
Figure 26. Robustness of intradermal immunization results in better memory. (A-C) Mice were
immunized i.d. or i.v. with 5x10 5 K bm1 mOva splenocytes. One month post-immunization, mice were
challenged i.v. with 5x10 5 K bm1 mOva splenocytes. On day 4 of the secondary response, mice were restimulated
in vivo by injecting CpG and SIINFEKL, as described above. Fifteen lymph nodes and the
spleen were harvested and K b -SIINFEKL tetramer-based enrichment combined with intracellular IFNγ
staining was performed. The experimental scheme is shown (A). The absolute number of tetramerpositive
cells is reported (B); and the percentage of IFNγ-producing cells among the population of
tetramer-positive cells was determined (C). Data points indicate a single mouse. Results are
representative of 2 independent experiments. NI, non-immunized animals during secondary
restimulation. p-values were calculated using a Mann-Whitney test.
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